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| 1 | bHLH121 Functions as a Direct Link that Facilitates the Activation of FIT by bHLH IVc Transcription Factors for Maintaining Fe Homeostasis in Arabidopsis显示文摘Iron(Fe)deficiency is prevalent in plants grown in neutral or alkaline soil.Plants have evolved sophisticated mechanisms that regulate Fe homeostasis,ensuring survival.In Arabidopsis,FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a crucial regulator of Fe-deficiency response.FIT is activated indirectly by basic helix-loop-helix(bHLH)IVc transcription factors(TFs)under Fed eficiency;how ever,it remains unclear which protein(s)act as the linker to mediate the activation of FIT by bHLH IVc TFs.In this study,we characterize the functions of bHLH121 and demonstrate that it directly associates with the FIT promoter.We found that loss-of-function mutations of bHLH121 cause severe Fedeficiency symptoms,reduced Feaccum ulation,and disrupted expression of genes associated with Fehomeostasis.Genetic analysis showed that FIT is epistatic to bHLH121 and FIT overexpression partially rescues the bhlh121 mutant.Further investigations revealed that bHLH IVc TFs interact with and promote nuclear accumulation of bHLH121.We demonstrated that bHLH121 has DNA-binding activity and can bind the prom oters of the FIT and bHLHlb genes,but we did not find that it has either direct transcriptional activation or repression activity tow ard these genes.Meanw hile,we found that bHLH121 functions downstream of and is a direct target of bHLH IVc TFs,and its expression is induced by Fe deficiency in a bHLH IV c-dependent manner.Taken together,these results establish that bHLH121 functions together with bHLH IVc TFs to positively regulate the expression of FIT and thus plays a pivotal role in maintaining Fe homeostasis in Arabidopsis. | Rihua Lei Yang Li Yuerong Cai Chenyang Li Mengna Pu Chengkai Lu Yujie Yang Gang Liang | 2020 | Molecular Plant2020，13，4： | 14 |
| 2 | Microstructure and wear resistance of highchromium cast iron containing niobium显示文摘In the paper, the effect of niobium addition on the microstructure, mechanical properties and wear resistance of high chromium cast iron has been studied. The results show that the microstructure of the heattreated alloys is composed of M7C3 and M23C6 types primary carbide, eutectic carbide, secondary carbide and a matrix of martensite and retained austenite. NbC particles appear both inside and on the edge of the primary carbides. The hardness of the studied alloys maintains around 66 HRC, not significantly affected by the Nb content within the selected range of 0.48%-0.74%. The impact toughness of the alloys increases with increasing niobium content. The wear resistance of the specimens presents little variation in spite of the increase of Nb content under a light load of 40 N. However, when heavier loads of 70 and 100 N are applied, the wear resistance increases with increasing Nb content. | Zhang Zhiguo Yang Chengkai Zhang Peng Li Wei | 2014 | China Foundry2014，11，3： | 4 |
| 3 | Oryza sativa FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(OsFIT/OsbHLH156)interacts with OsIRO2 to regulate iron homeostasis显示文摘Iron(Fe)is indispensable for the growth and development of plants.It is well known that FER-LIKE FE DEFICIENCY-INDUCED TRANSCRIPTION FACTOR(FIT)is a key regulator of Fe uptake in Arabidopsis.Here,we identify the Oryza sativa FIT(also known as Osb HLH156)as the interacting partner of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2(OsIRO2)that is critical for regulating Fe uptake.The OsIRO2 protein is localized in the cytoplasm and nucleus,but OsFIT facilitates the accumulation of OsIRO2 in the nucleus.Loss-of-function mutations of OsFIT result in decreased Fe accumulation,severe Fe-deficiency symptoms,and disrupted expression of Feuptake genes.In contrast,OsFIT overexpression promotes Fe accumulation and the expression of Fe-uptake genes.Genetic analyses indicate that OsFIT and OsIRO2 function in the same genetic node.Further analyses suggest that OsFIT and OsIRO2 form a functional transcription activation complex to initiate the expression of Fe-uptake genes.Our findings provide a mechanism understanding of how rice maintains Fe homeostasis. | Gang Liang Huimin Zhang Yang Li Mengna Pu Yujie Yang Chenyang Li Chengkai Lu Peng Xu Diqiu Yu | 2020 | Journal of Integrative Plant Biology2020，62，5： | 4 |
| 4 | Elevated CO2 differentially affects tobacco and rice defense against lepidopteran larvae via the jasmonic acid signaling pathway显示文摘Atmospheric CO_2 levels are rapidly increasing due to human activities. However, the effects of elevated CO_2(ECO_2) on plant defense against insects and the underlying mechanisms remain poorly understood. Here we show that ECO_2 increased the photosynthetic rates and the biomass of tobacco and rice plants, and the chewing lepidopteran insects Spodoptera litura and Mythimna separata gained less and more mass on tobacco and rice plants, respectively. Consistently, under ECO_2, the levels of jasmonic acid(JA), the main phytohormone controlling plant defense against these lepidopteran insects, as well as the main defense-related metabolites, were increased and decreased in insectdamaged tobacco and rice plants. Importantly, bioassaysand quantification of defense-related metabolites in tobacco and rice silenced in JA biosynthesis and perception indicate that ECO_2 changes plant resistance mainly by affecting the JA pathway. We further demonstrate that the defensive metabolites, but not total N or protein, are the main factors contributing to the altered defense levels under ECO_2. This study illustrates that ECO_2 changes the interplay between plants and insects, and we propose that crops should be studied for their resistance to the major pests under ECO_2 to predict the impact of ECO_2 on future agroecosystems. | Chengkai Lu Jinfeng qi Christian Hettenhausen Yunting Lei Jingxiong Zhang Mou Zhang Cuiping Zhang Juan Song Jing Li Guoyan Cao Saif ul Malook Jianqiang Wu | 2018 | Journal of Integrative Plant Biology2018，60，5： | 3 |
| 5 | Markerless human motion capture by Markov random field and dynamic graph cuts with color constraints显示文摘Currently,many vision-based motion capture systems require passive markers attached to key loca-tions on the human body. However,such systems are intrusive with limited application. The algorithm that we use for human motion capture in this paper is based on Markov random field (MRF) and dynamic graph cuts. It takes full account of the impact of 3D reconstruction error and integrates human motion capture and 3D reconstruction into MRF-MAP framework. For more accurate and robust performance,we extend our algorithm by incorporating color constraints into the pose estimation process. The ad-vantages of incorporating color constraints are demonstrated by experimental results on several video sequences. | LI Jia WAN ChengKai ZHANG DianYong MIAO ZhenJiang YUAN BaoZong | 2009 | Science in China(Series F)2009，52，2： | 2 |
| 6 | Structured Databases on the Web:Obervations and Implications显示文摘 | Chang K C C He Bin Li Chengkai | 2004 | ACM SIGMOD Record2004，33，3： | 1 |
| 7 | In situ TEM revealing the effects of dislocations on lithium-ion migration in transition metal dichalcogenides显示文摘The two-dimensional (2D) structure of layered transition metal dichalcogenides (TMDs) provides unusual physical properties [1,2]and chemical reactivity [3,4], which can be influenced by defects such as dislocations [5,6]. For example, dislocations can act as nucleation sites for the onset of deformation when subjected to stress [7]. | Ruiwen Shao Chengkai Yang Chen Yang Shulin Chen Weikang Dong Bairong Li Xiumei Ma Jing Lu Lixin Dong Peng Gao Dapeng Yu | 2021 | Journal of Energy Chemistry2021，30，7： | 1 |
| 8 | Structured Databases on the Web: Observations and Implications显示文摘 | Chang K C C He Bin Li Chengkai | 2004 | SIGMOD Record2004，33，3： | 1 |
| 9 | Structured database on the web:Observations and Implications显示文摘 | Chang K Chen-Chuan He Bin Li Chengkai | 2004 | SIGMOD Record2004，33，3： | 1 |
| 10 | Structured databases on the web显示文摘 | Kevin Chen-Chuan Chang Bin He Chengkai Li Mitesh Patel Zhen Zhang | 2004 | ACM SIGMOD Record2004，，3： | 1 |
| 11 | Structured databases on the Web: Observations and implementations 显示文摘 | Chang Kevin Chen-Chuan He Bin Li Chengkai | 2004 | ACM SIGMOD Record2004，33，3： | 1 |
| 12 | Structured Databases on the Web: Observations and Implications显示文摘 | Kevin C C He Bin Li Chengkai | 2004 | SIGMOD Record2004，33，3： | 1 |
| 13 | Structured databases on the web显示文摘 | Kevin Chen-Chuan Chang Bin He Chengkai Li Mitesh Patel Zhen Zhang | 2004 | ACM SIGMOD Record2004，，3： | 1 |
| 14 | Synthesis of Two Novel Additives and Study of Their Tribological Properties in Rapeseed Oil显示文摘二新奇的灰更少添加剂 - benzothiazole 衍生物包含硼和氯, OBC 和 BBC,被综合。在在油菜籽的添加剂上油的不同集体比率(RO ) 的 OBC 和 BBC 的 tribological 表演在一台四球的机器上被检验。油样品润滑的更低的钢球的穿的表面借助于扫描电子显微镜学(SEM ) 被分析。测试结果显示出那 OBC, BBC 在底有好溶解度上油,并且能有效地增加基础油的带负担的能力。包含 1.5 m% BBC 的油样品的最大的非抓住负担是 1117 N,它 2.3 倍于基础油的。OBC 和 BBC 能改进禁止性能和基础油的热稳定性的反穿和腐蚀,谁的起始的分解温度在 350 ° C 上面。然而,在不同集中的 OBC 和 BBC 能增加基础油的磨擦系数。油润滑的钢球的 SEM 形态学取样包含 1.5 m% 添加剂似乎比形成的基础油,和疤的更一致、光滑是很浅的。 | Zhou Maolin Li Fenfang Zeng Xiaojun Fan Chengkai | 2007 | China Petroleum Processing & Petrochemical Technology2007，9，3： | 1 |
| 15 | Struetured Databases on the Web: Observations and Implieations显示文摘 | Chang K C C He Bin Li Chengkai | 2004 | ACM SIGMOD Record2004，33，3： | 1 |
| 16 | Structured database on the web: Observations and Implications显示文摘 | Chang K Chen-Chuan He Bin Li Chengkai | 2004 | SIGMOD Record2004，33，3： | 1 |
| 17 | Initial exploration of tribological performance of novel triazine derivatives in water显示文摘 | Sheng Liping Li Fenfang Fan Chengkai | 2009 | Lubrication Science2009，21，： | 1 |
| 18 | Structured databases on the Web: observations and implications显示文摘 | Chang K C C He Bin Li Chengkai B | 2004 | ACM SIGMOD Record2004，33，3： | 1 |
| 19 | Initial exploration of tribological performance of novel triazine derivatives in water显示文摘 | SHENG LIP1NG LI FENFANG FAN CHENGKAI | 2009 | Lubrication Science2009，21，4： | 1 |
| 20 | Structured databases on the web: Observations and implications显示文摘 | Kevin Chenchuan He Bin Li Chengkai | 2004 | SIGMOD Record2004，33，3： | 1 |
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