|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Aquaporin-8 expression is reduced in ileum and induced in colon of patients with ulcerative colitis显示文摘AIM: To study susceptibility genes which may play a potential role in the pathogenesis and etiology of inflammatory bowel disease (IBD). METHODS: To identify potential susceptibility genes we performed global gene expression profiling in patients with IBD and control specimens. For determination of an intrinsic gene expression profile in ulcerative colitis (UC) and Crohn’s disease (CD) compared to normal subjects, mucosal biopsies of non-inflamed regions of the colon and the terminal ileum were subjected to DNA microarray analysis. Real-time RT-PCR and immunohistochemistry were used for verification of selected regulated candidate genes and a genetic analysis was performed. RESULTS: We could show that aquaporin-8 (AQP8) mRNA and protein levels were significantly increased in the colon of UC patients compared to controls. Genetic analysis of the six exons and the promoter region of AQP8, however, revealed no mutations or polymorphisms in IBD patients. CONCLUSION: Our results suggest that upregulation of AQP8 in the colon of UC patients represents a secondary phenomenon which may, due to altered water exchange of the distal intestinal mucosa, disturb the physiologic colonic mucus barrier and thus lead to chronic infla- mmation and ulceration. | Alexandra Zahn Christoph Moehle Thomas Langmann Robert Ehehalt Frank Autschbach Wolfgang Stremmel Gerd Schmitz | 2007 | World Journal of Gastroenterology2007，13，11： | 13 |
| 2 | Ephrin-B2 is differentially expressed in the intestinal epithelium in Crohn's disease and contributes to accelerated epithelial wound healing in vitro显示文摘AIM: Eph receptor tyrosine kinases and their membrane bound receptor-like ligands, the ephrins, represent a bi-directional cell-cell contact signaling system that directs epithelial movements in development. The meaning of this system in the adult human gut is unknown. We investigated the Eph/ephrin mRNA expression in the intestinal epithelium of healthy controls and patients with inflammatory bowel disease (IBD).METHODS: mRNA expression profiles of all Eph/ephrin family members in normal small intestine and colon were established by real-time RT-PCR. In addition, differential expression in IBD was investigated by cDNA array technology, and validated by both real-time RT-PCR and immunohistochemistry. Potential effects of enhanced EphB/ephrin-B signaling were analyzed in an in vitro IEC-6 cell scratch wound model.RESULTS: Human adult intestinal mucosa exhibits a complex pattern of Eph receptors and ephrins. Beside the known prominent co-expression of EphA2 and ephrinA1,we found abundantly co-expressed EphB2 and ephrin-B1/2.Interestingly, cDNA array data, validated by real-time PCR and immunohistochemistry, showed upregulation of ephrin-B2 in both perilesional and lesional intestinal epithelial cells of IBD patients, suggesting a role in epithelial homeostasis. Stimulation of ephrin-B signaling in ephrinB1/2 expressing rat IEC-6-cells with recombinant EphB1Fc resulted in a significant dose-dependent acceleration of wound closure. Furthermore, fluorescence microscopy showed that EphB1-Fc induced coordinated migration of wound edge cells is associated with enhanced formation of lamellipodial protrusions into the wound, increased actin stress fiber assembly and production of laminin at the wound edge.CONCLUSION: EphB/ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in IBD. | Christian Hafner Stefanie Meyer Thomas Langmann Gerd Schmitz Frauke Bataille Ilja Hagen Bernd Becker Alexander Roesch Gerhard Rogler Michael Landthaler Thomas Vogt | 2005 | World Journal of Gastroenterology2005，11，26： | 9 |
| 3 | Serum bile acid profiling reflects enterohepatic detoxification state and intestinal barrier function in inlammatory bowel disease显示文摘AIM:To determine free and conjugated serum bile acid (BA) levels in in? ammatory bowel disease (IBD) subgroups with defi ned clinical manifestations.METHODS: Comprehensive serum BA profiling was performed in 358 IBD patients and 310 healthy controls by liquid chromatography coupled to electrospray ionization tandem mass spectrometry.RESULTS: Serum levels of hyodeoxycholic acid, the CYP3A4-mediated detoxification product of the secondary BA lithocholic acid (LCA), was increased significantly in Crohn's disease (CD) and ulcerative colitis (UC), while most other serum BA species were decreased significantly. Total BA, total BA conjugate, and total BA glycoconjugate levels were decreased only in CD, whereas total unconjugated BA levels were decreased only in UC. In UC patients with hepatobiliary manifestations, the conjugated primary BAs glycocholic acid, taurocholic acid, and glycochenodeoxycholic acid were as significantly increased as the secondary BAs LCA, ursodeoxycholic acid, and tauroursodeoxycholic acid compared to UC patients without hepatobiliary manifestations. Finally, we found that in ileocecal resected CD patients, the unconjugated primary BAs, cholic acid and chenode-oxycholic acid, were increased significantly compared to controls and patients without surgical interventions.CONCLUSION: Serum BA profiling in IBD patients that indicates impaired intestinal barrier function and increased detoxification is suitable for advanced diagnostic characterization and differentiation of IBD subgroups with defined clinical manifestations. | Carsten Gnewuch Gerhard Liebisch Thomas Langmann Benjamin Dieplinger Thomas Mueller Meinhard Haltmayer Hans Dieplinger Alexandra Zahn Wolfgang Stremmel Gerhard Rogler Gerd Schmitz | 2009 | World Journal of Gastroenterology2009，15，25： | 4 |
| 4 | Lithocholic acid induction of the FGF19 promoter in intestinal cells is mediated by PXR显示文摘AIM: To study the effect of the toxic secondary bile acid lithocholic acid (LCA) on the expression of fibroblast growth factor 19 (FGF19) in intestinal cells and to characterize the pregnane-X-receptor (PXR) response of the FGF19 promoter region. METHODS: The intestinal cell line LS174T was stimulated with various concentrations of chenodeoxy-cholic acid and lithocholic acid for several time points. FGF19 mRNA levels were determined with quantitative realtime RT-PCR. FGF19 deletion promoter constructs were generated and the LCA response was analzyed in reporter assays. Co-transfections with PXR and RXR were carried out to study FGF19 regulation by these factors. RESULTS: LCA and CDCA strongly up-regulate FGF19 mRNA expression in LS174T cells in a time and dose dependent manner. Using reporter gene assays with several deletion constructs we found that the LCA responsive element in the human FGF19 promoter maps to the proximal regulatory region containing two poten-tial binding sites for PXR. Overexpression of PXR and its dimerization partner retinoid X receptor (RXR) and stimulation with LCA or the potent PXR ligand rifampicin leads to a signifi cant induction of FGF19 promoter activ-ity in intestinal cells. CONCLUSION: LCA induced feedback inhibition of bile acid synthesis in the liver is likely to be regulated by PXR inducing intestinal FGF19 expression. | Wolfgang Wistuba Carsten Gnewuch Gerhard Liebisch Gerd Schmitz Thomas Langmann | 2007 | World Journal of Gastroenterology2007，13，31： | 4 |
| 5 | Transpupillary thermotherapy for choroidal metastases显示文摘 | Ardjomand N Kucharczyc M Langmann G | 2001 | Ophthalmologica2001，215，： | 1 |
| 6 | Sta- ble Water Isotopes in Precipitation: A Regional Circula- tion Modeling Approach 显示文摘 | STURM K HOFFMANN G LANGMANN B | 2005 | Hydrology Process2005，19，： | 1 |
| 7 | Distribution and functional activity of P-glycoprotein and multidrug resistance-associated proteins in human brain microvascular endothelial cells in hippocampal sclerosis显示文摘 | KUBOTA H ISHIHARA H LANGMANN T | 2006 | Epilepsy Res2006，68，3： | 1 |
| 8 | A Si bipolar phase and frequency detector IC for clock extraction up to 8 Gb/s显示文摘 | Ansgar Pottb(a)cker Ulrich Langmann Hans-Ulrich Schreiber | 1992 | IEEE Journal of Solid-State Circuits1992，27，12： | 1 |
| 9 | Radiation sensitivity of visual and oeulomotor pathways显示文摘 | Leber KA Bergloff J Langmann G | 1995 | Ster- eotactic Funet Neurosurg1995，64，： | 1 |
| 10 | Molecular Clon-ing of the Human ATP-binding Cassette Transporter i (hABCI): Evidengce for Sterol-dependent Regulation in Macrophages 显示文摘 | Langmann T Klucken J Reil M | 1999 | Biochem Biophys Res Commun1999，257，： | 1 |
| 11 | A 1-G samplc/s 10-b full nyquist silicon bipolar track & hold IC显示文摘 | BAUMHEINRICH T PREGARDIER B LANGMANN U | 1997 | IEEE J of SSC1997，32，12： | 1 |
| 12 | Native LDL upregulation of ATP-binding cassette transporter-1 in human vascular endothelial cells显示文摘 | Liao H Langmann T Schmitz G | 2002 | Arterioscler Thromb Vasc Biol2002，22，1： | 1 |
| 13 | ZNF202 is inversely regulated with its target genes ABCA1 and apoE during macrophage differentiation and foam cell formation显示文摘 | Langmann T Schumacher C Morham SG | 2003 | J Lipid Res2003，44，5： | 1 |
| 14 | Role of ABCG1 and other ABCG family members in lipid metabolism显示文摘 | SCHMITZ G LANGMANN T HEIMERL S | 2001 | J Lipid Res2001，42，： | 1 |
| 15 | Adverse side effects with perfluorohexyloctane as a long-term tamponade agent in complicated vitreoretinal surgery显示文摘 | Schatz B El-Shabrawi Y Haas A Langmann G | 2004 | Retina2004，24，4： | 1 |
| 16 | Structure,function,and regulation of the ABCA1 gene product显示文摘 | Schmitz G Langmann T | 2001 | Curr Opin Lipidol2001，12，： | 1 |
| 17 | Radiation sensitivity of visual and oculomotor pathways显示文摘 | Leber K A Bergloff J Langmann G | 1995 | Stereotact Funct Neurosurg1995，64，1： | 1 |
| 18 | A 10-Gb/s CMOS Clock and Data Recovery Circuit with an Analog Phase Interpolator显示文摘 | Kreienkamp R Langmann U Zimmermann C | 2005 | IEEE Journal of Solid-State Circuits2005，40，3： | 1 |
| 19 | Mapping ATP-binding cassette transporter gene expression profiles in melanoeytes and melanoma cells显示文摘 | Heimerl S Bosserhoff AK Langmann T | 2007 | Melanoma Res2007，17，5： | 1 |
| 20 | Genome-wide expression profiling of the retinoschisinodeficient retina in early postnatal mouse development 显示文摘 | Gehrig A Langmann T Hoding F | 2007 | Invest Ophthalmol Vis Sei2007，48，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员