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| 1 | PI3K/Akt signaling transduction pathway is involved in rat vascular smooth muscle cell proliferation induced by apelin-13显示文摘脉管的光滑的肌肉房间(VSMC ) 被将生物的活组织移植于培养基中培养方法从男 SpragueDawley 老鼠的胸的主动脉准备经由 3 kinase (PI3K )/Akt 发信号 transduction 小径由 apelin-13 导致了的 phosphoinositide 观察 VSMC 增长。PI3K, phospho-PI3K, phospho-Akt, ERK1/2, phospho-ERK1/2 和 cyclin D1 的表示被西方的污点分析检测。结果证明 apelin-13 在剂量依赖者和时间依赖者举止支持了 phospho-PI3K 和 phospho-Akt 的表示。PI3K 禁止者 LY294002 显著地减少了 phospho-PI3K, phospho-Akt, phospho-ERK1/2,和 cyclin D1 的表示由 apelin-13 导致了。Akt 禁止者 1701-1 显著地减少了 phospho-Akt, phospho-ERK1/2,和 cyclin D1 的表示由 apelin-13 刺激了。MTT 试金结果证明 PI3K 禁止者 LY294002 和 Akt 禁止者 1701-1 显著地禁止了 apelin-13 导致的 VSMC 增长。Apelin-13 通过表明 transduction 小径的 PI3K/Akt 支持了 VSMC 增长。 | Changhui Liu Tao Su Fang Li Lanfang Li Xuping Qin Weinan Pan Fen Feng Feng Chen Duanfang Liao Linxi Chen | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 41 |
| 2 | The role of autophagy in cardiac hypertrophy显示文摘Autophagy 从更低的优核质在自然被保存到哺乳动物并且是一重要自我吃, degradative processthat 贡献过剩材料的消除。心脏的肥大被过量 proteinsynthesis,增加的 cardiomyocyte 尺寸,和变厚的室的墙首先描绘并且是支持 arrhythmiaand 心失败的一个主要风险因素。在最近的年里, cardiomyocyte autophagy 被认为在控制 hypertrophicresponse 起一个作用。然而,在心脏的肥大的 cardiomyocyte autophagy 的有益或加重的角色仍然是在心脏的肥大的 cardiomyocyte autophagy 的准确机制进一步要求的 controversial.The 学习。在这评论,我们在心脏的肥大与 autophagy 联系的 summarizethe 争吵并且在心脏的肥大的 thedevelopment 提供卓见进 autophagy 的角色。我们断定未来研究应该强调在 autophagy andthe 之间的关系心脏的肥大的不同阶段,以及 autophagic 流动和选择 autophagy。Autophagy 将是为心脏的肥大的一个 potentialtherapeutic 目标。 | Lanfang Li Jin Xu Lu He Lijun Peng Qiaoqing Zhong Linxi Chen Zhisheng Jiang | 2016 | Acta Biochimica et Biophysica Sinica2016，48，6： | 30 |
| 3 | 14-3-3 mediates apeHn-13-induced enhancement of adhesion of monocytes to human umbilical vein endothelial cells显示文摘调查 apelin-13 是否导致了 THP-1 单核白血球(MC ) 对经由表明 transduction 小径和潜在的小说的 14-3-3 的 ECV304 人的脐的静脉 endothelial 房间(HUVEC ) 的粘附生理的功能和 apelin-APJ 的发信号的 transduction 小径, HUVEC ECV304 在 DMEM 和 MC THP-1 是有教养的在 RPMI 1640 是有教养的中等。单核白血球粘附和脉管的房间粘附 molecule-1 (VCAM-1 ) 和 14-3-3 的表示与单核白血球粘附试金和西方的污点分析被测量。数据证明 apelin-13 在一个集中依赖者和时间依赖者举止增加了 MC 的粘附到 HUVEC,它分别地在 1 M 和 12 h 到达了他们的山峰。同样, apelin-13 导致了 HUVEC 粘附分子的表示,在一个集中依赖者和时间依赖者举止, VCAM-1 分别地在 1 M 和 12 h 到达了他们的山峰。Apelin-13 在一个集中依赖者和时间依赖者举止导致了 14-3-3 的表示,它分别地在 1 M 和 5 min 到达了他们的山峰。而且,有势力 14-3-3 禁止者 difopein 显著地减少了 14-3-3 的表示,在 apelin-13 的 VCAM-1 刺激了 HUVEC,并且 difopein 显著地在对 HUVEC 的 MC 粘附的正式就职上禁止了 apelin-13 的效果。这些数据建议 14-3-3 调停了到由 Apelin-13 的 HUVEC 的 MC 的粘附的正式就职。 | Xin Li Xianhui Zhang Fang Li Linxi Chen Lanfang Li Xuping Qin Jing Gao Tao Su Yixin Zeng Duanfang Liao | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 21 |
| 4 | Jagged-1/Notch3 signaling transduction pathway is involved in apelin-13-induced vascular smooth muscle cells proliferation显示文摘apelin/apelin 受体(APJ, apelinangiotensin 像受体 1 ) 系统是一最新 deorphanized G 联合蛋白质的受体系统。是重要规章的因素的 apelin 和 APJ 在心血管的系统被表示。我们的以前的研究显著地表明了那 apelin-13 刺激脉管的光滑的肌肉房间(VSMC ) 增长。在这份报纸,我们的数据建议表明 transduction 小径的 Jagged-1/Notch3 被支持 Cyclin D1 的表示涉及 apelin-13-induced VSMC 增长。结果显示 apelin-13 在集中依赖者和时间依赖者礼貌刺激 VSMC 和 Jagged-1 和 Notch3 的表示的增长。apelin-13 导致的 Jagged-1 和 Notch3 的增加的表示能被细胞外的调整信号的蛋白质 kinase (英皇家空军之阶级最低之兵) 废除封锁。PD98059 (英皇家空军之阶级最低之兵禁止者) 能禁止 apelin-13 导致的 Jagged-1/Notch3 的激活。用小介入 RNA 的 Notch3 的下面规定禁止 Cyclin D1 的表示并且阻止 apelin-13-induced VSMC 增长。在结论, Jagged-1/Notch3 发信号 transduction 小径涉及 apelin-13 导致的 VSMC 增长。 | Lifang Li Lanfang Li Feng Xie Zidong Zhang Yu Guo Guotao Tang Deguan Lv Qixuan Lu Linxi Chen Jian Li | 2013 | Acta Biochimica et Biophysica Sinica2013，45，10： | 18 |
| 5 | ERK1/2 mediates lung adenocarcinoma cell proliferation and autophagy induced by apelin-13显示文摘这研究的目的是在房间增长和肺腺癌的 autophagy 调查 apelin 的角色。在肺腺癌的 APJ 的在表示上被 immunohistochemistry 检测,当在肺癌症病人的血浆 apelin 水平被连接酶的 immunosorbent 试金测量时。我们的调查结果表明 apelin-13 显著地增加了 ERK1/2 的 phosphorylation, cyclin D1 的表示,联系微导管的蛋白质 1 轻链 3A/B (LC3A/B ) ,和 beclin1,并且证实 apelin-13 支持了 A549 房间增长并且经由 ERK1/2 发信号导致了 A549 房间 autophagy。而且,人的肺腺癌房间线 A549 的表面和光滑、光滑的 apelin-13 原因房间表面上有毛孔是在原子力量下面观察了显微镜学。这些结果建议 ERK1/2 发信号小径调停 apelin-13-induced 肺腺癌房间增长和 autophagy。在我们的试验性的条件下面,与 3-methyladenine 联系的 autophagy 不涉及房间增长。 | Li Yang Tao Su Deguan Lv FengXie Wei Liu Jiangang Cao Irshad Ali Sheikh Xuping Qin Lanfang Li Linxi Chen | 2014 | Acta Biochimica et Biophysica Sinica2014，46，2： | 15 |
| 6 | miR-503-3p promotes epithelialemesenchymal transition in breast cancer by directly targeting SMAD2 and E-cadherin显示文摘Although progress in clinical and basic research has significantly increased our understanding of breast cancer, little is known about the molecular mechanism underlying breast cancer metastasis. Identification of effective therapeutic targets to prevent breast cancer metastasis is urgently needed. The function of mi R-503-3p has been investigated in other cancers, but its role in breast cancer remains undefined.Here, we found that mi R-503-3p was overexpressed in breast cancer tissue and plasma compared with adjacent normal breast tissue and with plasma from healthy individuals. Moreover, we identified mi R-503-3p to be an oncogene of breast cancer cell proliferation, migration and invasion. Upregulation of mi R-503-3p in breast cancer cells inhibited expression of epithelialemesenchymal transition(EMT)-related protein SMAD2 and the epithelial marker protein E-cadherin by directly binding to their m RNA30 untranslated region, whereas increased expression of mesenchymal marker proteins, including vimentin and N-cadherin. Taken together, our findings support a critical role for mi R-503-3p in induction of breast cancer EMT and suggest that plasma mi R-503-3p may be a useful diagnostic biomarker for breast cancer. | Zitong Zhao Xinyi Fan Lanfang Jiang Zhongqiu Xu Liyan Xue Qimin Zhan Yongmei Song | 2017 | Journal of Genetics and Genomics2017，44，2： | 15 |
| 7 | NEK7: a novel promising therapy target for NLRP3-related inflammatory diseases显示文摘 | Jin Xu Liqun Lu Lanfang Li | 2016 | Acta Biochimica et Biophysica Sinica2016，48，10： | 12 |
| 8 | A static pressure sensitive receptor APJ promote H9c2 cardiomyocyte hypertrophy via PI3K-autophagy pathway显示文摘这研究被设计调查 APJ 受体是否在静态的导致压力的 cardiomyocyte hypertrophyand 充当一个传感器调查 PI3K-autophagy 小径的机制。左室的肥大老鼠模型是腹的主动脉的确定的 bycoarctation。H9c2 老鼠 cardiomyocytes 面对被给 bya 的静态的压力是有教养的定做的压力孵卵器。结果表明 apelin/APJ 系统, PI3K, Akt 和他们的 phosphorylationwere 的表达式显著地在操作组增加了。静态的压力起来调整在 cardiomyocytes 的 APJ 表示, PI3K phosphorylation, Akt phosphorylation, LC3-II/I 和 beclin-1 表示。APJ shRNA pGPU6/Neo-rat-399, PI3K 禁止者 LY294002, Akt 禁止者 1701-1 分别地堵住了 APJ, PI3K phosphorylation, Akt phosphorylation, LC3-II/I 和 beclin-1 表示的起来规定。而且,静态的压力增加了直径,体积,房间的蛋白质内容,和这些能是 reversedwhen 房间分别地与 pGPU6/Neo-rat-399, LY294002,和 autophagy 禁止者 3-methyladenine 被对待。Theseresults 建议了那静态的压力起来调整 APJ 表情由 PI3K-autophagypathway 支持 cardiomyocyte 肥大。 | Feng Xiei Wei Liu Fen Feng Xin Li Li Yang Deguan Lv Xuping Qin Lanfang Li Linxi Chen | 2014 | Acta Biochimica et Biophysica Sinica2014，46，8： | 10 |
| 9 | Hypoxia promotes bone marrow-derived mesenchymal stem cell proliferation through apelin/APJ/autophagy pathway显示文摘骨头导出髓的间充质的干细胞(BMSC ) 是有增长和区别的能力进间充质的系房间的 multipotent 祖先的一张人口。规章的肽 apelin 是为 G 联合蛋白质的受体 APJ 的内长的 ligand。Apelin,能提高 BMSC 增长,在许多房间类型上有 mitogenic 效果。我们假设了增加的 apelin/APJ 可能涉及导致组织缺氧的 BMSC 增长的出现和发展。从到 10-week-old C57BL/6J 老鼠的 8- 的骨头髓的 BMSC 在 normoxia (21% 氧) 或组织缺氧(1% 氧) 下面是有教养的状况。房间增长被 3-(4,5-dimethylthiazol-2-yl ) 决定 -2,5-diphenyltetrazolium 溴化物试金和 5-bromo-2-deoxyuridine 试金。组织缺氧可诱导的因素(HIF ) 的表情 -1, apelin, APJ, Beclin-1,和 LC3II/LC3I 被西方的污点分析检测。结果建议组织缺氧以一种时间依赖者方式提高了 BMSC 的增长。HIF-1, apelin, APJ, Beclin-1,和 LC3II/LC3I 的表情在组织缺氧导致的 BMSC 被增加。禁止了 apelin, APJ, Beclin-1,和 LC3II/LC3I 的导致组织缺氧的表情的小介入 RNA (siRNA )-HIF-1 阻止了导致组织缺氧的 BMSC 增长。禁止了 Beclin-1 和 LC3II/LC3I 的导致组织缺氧的表情的 siRNA-APJ 颠倒了导致组织缺氧的 BMSC 增长。siRNA-Beclin-1 也废除了导致组织缺氧的房间增长。这些数据建议表明小径的 apelin/APJ/autophagy 可能涉及导致组织缺氧的 BMSC 增长。 | Lanfang Li Lifang Li Zidong Zhang Zhisheng Jiang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，5： | 10 |
| 10 | Apelin-13 promotes cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy显示文摘Apelin 高度在左室的肥大 Sprague Dawley 老鼠建模的老鼠被表示,并且它在心血管的系统起一个关键作用。这研究是澄清 apelin-13 是否在 H9c2 老鼠 cardiomyocytes 支持肥大并且调查它的内在的机制的目的。cardiomyocyte 肥大被测量 H9c2 房间的直径,体积,和蛋白质内容观察。autophagy 的激活被由传播电子显微镜学观察 autophagosomes 的形态学评估,观察由轻显微镜学的 LC3 的 subcellular 本地化,并且由西方的污点分析检测 LC3 的联系膜的形式。表明小径的 phosphatidylinositol 3-kinase (PI3K ) 被识别,蛋白质表示用西方的污点分析被检测。结果表明 apelin-13 增加了 H9c2 房间的直径,体积,和蛋白质内容并且支持了 PI3K, Akt, ERK1/2,和 p70S6K 的 phosphorylation。Apelin-13 激活 PI3K-Akt-ERK1/2-p70S6K 小径。PI3K 禁止者 LY294002, Akt 禁止者 1701-1, ERK1/2 禁止者 PD98059 稀释了房间直径的增加,体积,蛋白质内容由 apelin-13 导致了。Apelin-13 增加了 autophagosomes 并且起来调整 beclin 的表情 1 并且 LC3-II/I 短暂地并且稳定地。autophagy 禁止者 3MA 改善了被 apelin-13 导致的房间直径,体积,和蛋白质内容的增加。这些结果建议 apelin-13 经由 PI3K-Akt-ERK1/2-p70S6K 和导致 PI3K 的 autophagy 支持 H9c2 老鼠 cardiomyocyte 肥大。 | Feng Xie Wei Liu Fen Feng Xin Li Lu He Deguan Lv Xuping Qin Lifang Li Lanfang Li Linxi Chen | 2015 | Acta Biochimica et Biophysica Sinica2015，47，12： | 9 |
| 11 | The relationship between internal domain sequences ofpiggyBac and its transposition efficiency in BmN ceils and Bombyx mori显示文摘piggyBac transposon,它包括终端转换了重复序列和内部领域(标志) 序列,广泛地为昆虫转变被用作一个工具。为关于 Bombyx mori 的转基因的研究优化这个系统,我们在绿色的表达式上检验了 transposase plasmid 和它的标志序列的数量的效果荧光灯蛋白质(GFP ) 。四种 transposon plasmids , pB [ A3GFP ]有标志的完整的长度的 -1 定序, pB [ A3GFP ]有 3 仅仅标志顺序的 -2 , pB [ A3GFP ]没有标志序列的 -3 ,和 pB [ A3GFP ]包含 5 标志顺序的 333 bp 的 -4 ,并且 3 标志顺序的 179 bp 作为标记与 GFP 被构造。在 transfecting 以后这四 plasmids 进 BmN 房间,我们由比较对否定房间比率积极的 GFP 分析了 transfecting 效率。我们的结果显示 plasmid pB-4 在第 22 天得到了最高的比率。而且,没有生产过剩抑制,对否定房间比率积极的 GFP 与 transposase plasmid 的更高的数量增加了。而且,我们注射了三 piggyBac transposon plasmids, pB [A3GFP ]-1, pB | Lanfang Zhuang Hao Wei Changde Lu Boxiong Zhong | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 7 |
| 12 | Elabela, a new endogenous ligand of APJ, functions in embryos and adults organisms显示文摘 | Liqun Lu Jiangang Cao Lanfang Li Linxi Chen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，4： | 7 |
| 13 | Newborn screening with targeted sequencing:a multicenter investigation and a pilot clinical study in China显示文摘Different newborn screening(NBS) programs have been practiced in many countries since the 1960 s. It is of considerable interest whether next-generation sequencing is applicable in NBS. We have developed a panel of 465 causative genes for 596 early-onset, relatively high incidence, and potentially actionable severe inherited diseases in our Newborn Screening with Targeted Sequencing(NESTS) program to screen 11,484 babies in 8 Women and Children’s hospitals nationwide in China retrospectively. The positive rate from preliminary screening of NESTS was 7.85%(902/11,484). With 45.89%(414/902) follow-up of preliminary positive cases, the overall clinically confirmative diagnosis rate of monogenic disorders was 12.07%(50/414), estimating an average of 0.95%(7.85% × 12.07%) clinical diagnosis rate, suggesting that monogenic disorders account for a considerable proportion of birth defects. The disease/gene spectrum varied in different regions of China. NESTS was implemented in a hospital by screening 3923 newborns to evaluate its clinical application. The turn-around time of a primary report, including the sequencing period of < 7 days, was within 11 days by our automatic interpretation pipeline. Our results suggest that NESTS is feasible and cost-effective as a first-tier NBS program, which will change the status of current clinical practice of NBS in China. | Chanjuan Hao Ruolan Guo Xuyun Hu Zhan Qi Qi Guo Xuanshi Liu Yuanhu Liu Yanhua Sun Xiaofen Zhang Feng Jin Xiujie Wu Ren Cai Dingyuan Zeng Xijiang Hu Xiaohua Wang Xiaoping Ji Wenjie Li Quansheng Xing Lanfang Mu Xiulian Jiang Xue Yang Weimin Yang Yan Zhang Qianli Yin Xin Ni Wei Li | 2022 | Journal of Genetics and Genomics2022，49，1： | 7 |
| 14 | Downregulation of miR-503 Promotes ESCC Cell Proliferation,Migration,and Invasion by Targeting Cyclin D1显示文摘Esophageal squamous cell carcinoma(ESCC) is one of the most aggressive cancers in China,but the underlying molecular mechanism of ESCC is still unclear.Involvement of microRNAs has been demonstrated in cancer initiation and progression.Despite the reported function of miR-503 in several human cancers,its detailed anti-oncogenic role and clinical significance in ESCC remain undefined.In this study,we examined miR-503 expression by q PCR and found the downregulation of miR-503 expression in ESCC tissue relative to adjacent normal tissues.Further investigation in the effect of miR-503 on ESCC cell proliferation,migration,and invasion showed that enhanced expression of miR-503 inhibited ESCC aggressive phenotype and overexpression of CCND1 reversed the effect of miR-503-mediated ESCC cell aggressive phenotype.Our study further identified CCND1 as the target gene of miR-503.Thus,miR-503 functions as a tumor suppressor and has an important role in ESCC by targeting CCND1. | Lanfang Jiang Zitong Zhao Leilei Zheng Liyan Xue Qimin Zhan Yongmei Song | 2017 | Genomics, Proteomics & Bioinformatics2017，15，3： | 5 |
| 15 | Elabela-APJ axis contributes to embryonic development and prevents pre-eclampsia in pregnancy显示文摘 | Qionglin Zhou Kai Zhang Yu Guo Linxi Chen Lanfang Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，3： | 5 |
| 16 | Autophagy, a new target for disease treatment显示文摘Since Ashford and Porter first discovered that cells 'eat themselves' in 1962, the process known as autophagy has generated intense interest in both biomedical research and clinical medicine. Autophagy is a regulated cellular pathway that transports damaged, modified or aging organelles and proteins to lysosomes/vacuoles for degradation. | XIE Feng LI LanFang CHEN LinXi | 2013 | Science China(Life Sciences)2013，56，9： | 5 |
| 17 | The TBK1-OPTN Axis Mediates Crosstalk Between Mitophagy and the Innate Immune Response: A Potential Therapeutic Target for Neurodegenerative Diseases显示文摘Recently,Richter et al.[1]revealed the potential functions of the interaction between the serine/threonine kinase Tank-binding kinase 1(TBK1)and the autophagy receptor optineurin(OPTN).The TBK1-OPTN axis targets damaged mitochondria for degradation via PINK1/parkin-mediated mitophagy[2,3].Indeed,TBK1 can phosphorylate OPTN at Ser177,Ser473,or Ser513 to enhance the binding capacity of OPTN with poly-ubiquitin(poly-UB)chains.Conversely,binding of poly-UB chains to OPTN | Lu He Linxi Chen Lanfang Li | 2017 | Neuroscience Bulletin2017，33，3： | 5 |
| 18 | Polysaccharides in Sipunculus nudus: Extraction Condition Optimization and Antioxidant Activities显示文摘Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides(SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60℃, solid-liquid ratio 1:6 g mL^(-1), duration 5 h, and alkali(Na OH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant. | ZHANG Qin DONG Lanfang TONG Tong WANG Qingchao XU Mingzhu | 2017 | Journal of Ocean University of China2017，16，1： | 5 |
| 19 | miR-10a inhibits cell proliferation and promotes cell apoptosis by targeting BCL6 in diffuse large B-cell lymphoma显示文摘BCL6 (B 房间淋巴瘤 6 ) 基因是经常在弥漫的大 B 房间淋巴瘤(DLBCL ) 被表示的 proto-oncogene。功能的 BCL6 损失能杀死 DLBCL 房间,证明 BCL6 为 DLBCL 房间的幸存是必要的并且能是一个治疗学的目标。在这研究,我们发现那 BCL6 蛋白质层次,建议那是一致地在 DLBCL 纸巾的 upregulated 而它的 mRNA 层次在纸巾随机变化了, post-transcriptional 机制涉及 BCL6 规定。我们使用了生物信息学分析寻找 miRNAs,它潜在地指向 BCL6,并且在 BCL6 的 3-untranslated 区域(3-UTR ) 为 miR-10a 识别了特定的指向的地点。我们进一步鉴别在 miR-10a 层次和 BCL6 蛋白质层次之间的反的关联,然而并非 mRNA 铺平,在 DLBCL 肿瘤织物样品。由 overexpressing 或在 DLBCL 房间将 miR-10a 击倒,我们试验性地直接验证了那 miR-10a 认出 BCL6 抄本和调整 BCL6 表示的 3-UTR。而且,我们证明否定地由 miR-10a 调整 BCL6 压制了增长并且支持了 DLBCL 房间的 apoptosis。 | Qian Fan Xiangrui Meng Hongwei Liang Huilai Zhang Xianming Liu Lanfang Li Wei Li Wu Sun Haiyang Zhang Ke Zen Chen-Yu Zhang Zhen Zhou Xi Chen Yi Ba | 2016 | Protein & Cell2016，7，12： | 5 |
| 20 | Impact of biochar amendment on soil aggregation varied with incubation duration and biochar pyrolysis temperature显示文摘Soil aggregation is one of the crucial processes that facilitate carbon sequestration and maintain soil fertility.So far,the effect of biochar amendment on soil aggregation remains inconclusive.Here,we tested the hypothesis that the response of soil aggregation to biochar addition varied with incubation duration and biochar chemistry.A one year microcosm experiment of soil with biochar was conducted that included biochar produced at three different temperatures(300,450,and 600°C),and three biochar application rates,i.e.,0,1,and 3 wt%.It was observed that after one and three months,biochar mainly(>90%)distributed in the micro-aggregates,and slightly reduced aggregate stability and increased proportion of micro-aggregates,which was demonstrated to result from the mechanical mixture of amended biochar with soil.Contrastingly,when the duration was prolonged to six months and one year,a significant increase in macro-aggregates(6.6-38.5%)and aggregate stability(7.3-29.4%)was detected,with the increasing extent being apparently higher for low-temperature biochar.This was related to the comparatively strong interaction of biochar particles with soil minerals or microbes after long-time incubation.The strong interaction was directly supported by the significant increase in H/C,O/C ratios of isolated biochar from treated soils,the detection of typical soil mineral elements on the surface of isolated biochar,and the increase in microbial biomass carbon of treated soils.The findings of this study highlighted the role of biochar type and amendment duration in mediating the effect of biochar application on soil aggregation. | Lanfang Han Biao Zhang Liying Chen Yanfang Feng Yan Yang Ke Sun | 2021 | Biochar2021，3，3： | 4 |
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