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| 1 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 2 | Determination of specific activity of recombinant hirudin using a thrombin titration method 显示文摘 | Lalitha L Jawed F | 1995 | Thromb Res1995，78，3： | 1 |
| 3 | Wavelet analysis for classification of multi-source PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Trans on Dielectrics and Electrical Insulation2000，7，1： | 1 |
| 4 | Effect of packaging atmosphere on the microbial attributes of pearlspot( Etroplus suratensis Bloch)stored at 0 ℃ - 2℃ 显示文摘 | Ravi Sankar C N Lalitha K V Jose L | 2008 | Food Microbiology2008，25，3： | 1 |
| 5 | Wavelet analysis for classification of multi-source PD patterns 显示文摘 | LALITHAE M SATISH L | 2000 | IEEE Trans- actions on Dielectrics and Electrical Insulation2000，7，1： | 1 |
| 6 | Effects of sodium acetate dip treatment and vacuum-packaging on chemical, microbiological, textural and sensory changes of pearlspot(Etroplus suratensis ) during chill storage 显示文摘 | Manju S Jose L Gopal T K S Ravishankar C N Lalitha K V | 2007 | Food Chemistry2007，102，7： | 1 |
| 7 | Wavelet analysis for classification of multi-source PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Trans on D&EI2000，7，1： | 1 |
| 8 | Wavelet analysis for classification of mul- ti-source PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Transactions on Dielectrics and Electrical Insulation2000，1，7： | 1 |
| 9 | Sensitivity of Filamentous Fungi Isolated From Fungal Keratitis to Amphotericin B, Natamycin, Caspofungin, Itraconazole, Voriconazole, and Posaconazole显示文摘 | B L Shapiro P Lalitha A W Fothergill | 2005 | Invest Ophthalmol Vis Sci2005，46，5： | 1 |
| 10 | Production of the subdomains of the Plasmodium falciparum apical membrane antigenl ectodomain and analysis of the immune response显示文摘 | Lalitha P V Ware L A Barbosa A | 2004 | Infect Immun2004，72，： | 1 |
| 11 | Managerial incentives and risk taking显示文摘 | Coles Jeffrey L Naveen D.Daniel Lalitha Naveen | | 0，，79： | 1 |
| 12 | Effect of packaging atmosphere on the microbial attributes of pearlspot (Elroplus sttratensis Bloch) stored at 0-2℃ 显示文摘 | Ravi Sankar C N Lalitha K V Jose L | 2009 | Food Microbiology2009，25，3： | 1 |
| 13 | Wavelet analysis for classification of multi-souce PD patterns显示文摘 | L Satish | 2000 | IEEE Transactions on Dielectrics and Electrical Insulation2000，7，1： | 1 |
| 14 | Antimicrobial susceptibility of Fusarium, Aspergillus, and other filamentous fungi isolated from keratitis显示文摘 | Lalitha P Shapiro B L Srinivasan M | 2007 | Arch Ophthalmol2007，125，6： | 1 |
| 15 | Effect ofpackaging atmosphere on the microbial attributes of pearlspot {Etroplussuratensis Bloch)stored at 0-2 °C显示文摘 | RAVI SANKAR C N LALITHA K V JOSE L | 2009 | Food Microbiology2009，25，3： | 1 |
| 16 | Fractal Image Compression for Classification of PD Sources 显示文摘 | Lalitha E M Satish L | 1998 | IEEE Trans Dielect Elect Insul1998，5，： | 1 |
| 17 | Fractal image compression for classification of PD sources显示文摘 | Lalitha E M and Satish L | 1998 | IEEE Trans on Dielectrics and Electrical Insulation1998，5，4： | 1 |
| 18 | Managerial Incentives and Risk - taking 显示文摘 | Coles Jeffrey L Naveen D Daniel Lalitha Naveen | 2006 | Journal of Financial Economics2006，，79： | 1 |
| 19 | Wavelet analysis for classification of multi-souce PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Transactions on Dielectrics and Electrical Insulation2000，7，1： | 1 |
| 20 | A nanoplex PCR assay for the rapid detection of vancomycin and bifunctional aminoglycoside resistance genes in Enterococcus species显示文摘 | Yean Yean C Su Yin L Lalitha P | | BMC Microbiol0，7，1： | 1 |
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