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| 1 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 2 | Antimic robial suscepti- bility of fusarium, aspergillus, and other filamentous fungi isolated from keratitis 显示文摘 | Lalitha P Shapiro BL Srinivasan M | 2007 | Arch Ophthalmol2007，125，6： | 1 |
| 3 | Postcataract endophthalmitis in South India incidence and outcome 显示文摘 | LALITHA P RAJAGOPALAN J PRAKASH K RAMASAMY K PRAJNA NV SRINIVASAN M | 2005 | Ophthalmology2005，112，11： | 1 |
| 4 | 显示文摘 | Vishu Kumar A B VAradaraj M C Lalitha R G | 2004 | Biochimicaer Biophysica Acta2004，1670，2： | 1 |
| 5 | Wavelet analysis for classification of multi-source PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Trans on Dielectrics and Electrical Insulation2000，7，1： | 1 |
| 6 | Seasonal trends of micro- bial keratitis in South India 显示文摘 | Lin CC Lalitha P Srinivasan M | 2012 | Cornea2012，31，10： | 1 |
| 7 | Low molecular weight chitosans: Preparation with the aid of papain and characterization显示文摘 | VISHU KUMAR A B VARADARAJ M C LALITHA R G | 2004 | Bioehimiea et Biophysiea Aeta ( BBA ) : General Subjects2004，1670，2： | 1 |
| 8 | Bacteriophages for aquaculture : Are they beneficial or inimical显示文摘 | Rao B M Lalitha K V | 2015 | Aquaculture2015，437，： | 1 |
| 9 | Wavelet analysis for classification of multi-source PD patterns 显示文摘 | LALITHAE M SATISH L | 2000 | IEEE Trans- actions on Dielectrics and Electrical Insulation2000，7，1： | 1 |
| 10 | Vector variational inequalities with cone-pseudomonotone bifunctions显示文摘 | LALITHA C S MEHTA M | 2005 | Optimization2005，54，3： | 1 |
| 11 | Wavelet analysis for classification of multi-source PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Trans on D&EI2000，7，1： | 1 |
| 12 | Wavelet analysis for classification of mul- ti-source PD patterns显示文摘 | Lalitha E M Satish L | 2000 | IEEE Transactions on Dielectrics and Electrical Insulation2000，1，7： | 1 |
| 13 | A validated stability-indicatingRP-HPLC method for levofloxacin in the presence of degradationproducts,its process related impurities and identification of oxidati-ve degradant显示文摘 | Chandrasekhar KB M Lalitha Devi | 2009 | J Pharmaceut Biomed2009，50，： | 1 |
| 14 | Seasonal trends of microbial keratitis in south India显示文摘 | Lin CC Lalitha P Srinivasan M | 2012 | Cornea2012，31，10： | 1 |
| 15 | Comparison of phenotypic versus genotypic methods in the detection of methicillin resistance in staphylococcus aureus 显示文摘 | MOHANASOUNDARAM K M LALITHA M K | 2008 | Indian J Med Res2008，127，1： | 1 |
| 16 | Rapid diagnosisof acute pyogenic meningitis by a combined PCR dot-blotassay显示文摘 | Balganesh M Lalitha M K N athaniel R | 2000 | Mol Cell Probes2000，14，2： | 1 |
| 17 | Incidence of Hae- mophilus influenzae type b meningitis in India显示文摘 | MINZ S BALRAJ V LALITHA M K | 2008 | Indian J Med Res2008，128，1： | 1 |
| 18 | Acanthamoeba keratitis in South India : a longitudinal analysis of epidemics 显示文摘 | Lalitha P Lin CC Srinivasan M | 2012 | Ophthalmic Epidemiol2012，19，： | 1 |
| 19 | Comparison of phenotypic versus genotypic method in the detection of methicillin resistance in staphylococcus aureus 显示文摘 | Mohanasoundaram K M Lalitha M K | 2008 | Indian J Med Res2008，127，1： | 1 |
| 20 | Cortieosteroids for bacteria/corneal ulcers显示文摘 | Srinivasan M Lalitha P Mahalakshmi R | 2009 | Br J Ophthalmol2009，93，2： | 1 |
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