| 3 | Distribution,structure and biosynthetic gene families of(1,3;1,4)-β-glucan in Sorghum bicolor显示文摘In cereals, the presence of soluble polysaccharides including(1,3;1,4)-b-glucan has downstream implications for human health, animal feed and biofuel applications. Sorghum bicolor(L.) Moench is a versatile crop, but there are limited reports regarding the content of such soluble polysaccharides.Here, the amount of(1,3;1,4)-b-glucan present in sorghum tissues was measured using a Megazyme assay. Very low amounts were present in the grain, ranging from 0.16%–0.27%(w/w), while there was a greater quantity in vegetative tissues at 0.12–1.71%(w/w). The fine structure of(1,3;1,4)-b-glucan, as denoted by the ratio of cellotriosyl and cellotetraosyl residues,was assessed by high performance liquid chromatography(HPLC) and ranged from 2.6–3:1 in the grain, while ratios in vegetative tissues were lower at 2.1–2.6:1. The distribution of(1,3;1,4)-b-glucan was examined using a specific antibody and observed with fl uorescence and transmission electron microscopy. Micrographs showed a variable distribution of(1,3;1,4)-b-glucan in fl uenced by temporal and spatial factors. The sorghum orthologs of genes implicated in the synthesis of(1,3;1,4)-b-glucan in other cereals, such as the Cellulose synthase-like(Csl) F and H gene families were de fined.Transcript pro filing of these genes across sorghum tissues was carried out using real-time quantitative polymerase chain reaction, indicating that, as in other cereals, Csl F6 transcripts dominated. | Riksfardini A.Ermawar Helen M.Collins Caitlin S.Byrt Natalie S.Betts Marilyn Henderson Neil J.Shirley Julian Schwerdt Jelle Lahnstein Geoffrey B.Fincher Rachel A.Burton | 2015 | Journal of Integrative Plant Biology2015，57，4： | 2 |
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