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| 1 | In vitro differentiation of human umbilical cord Wharton's jelly mesenchymal stromal cells to insulin producing clusters显示文摘AIM: To investigate the differentiation of human Wharton's jelly derived mesenchymal stromal cells(WJMSCs) to insulin producing clusters(IPC) this study was conducted. METHODS: The umbilical cords samples were collected from full term caesarian section mothers and the WJ-MSCS were cultured from tissue explants in High glucose-Dulbecco's Modified Eagle Medium(H-DMEM); H-DMEM supplemented with 10% fetal bovine serum(FBS) and antibiotics. The expression of CD90, CD44, CD105, CD34 and CD133 as well as osteogenic and adipogenic differentiation of cells in appropriate medium were also evaluated. The cells were differentiated toward IPC with changing the culture medium and adding the small molecules such as nicotinic acid, epidermal growth factor, and exendin-4 during 3 wk period. The gene expression of PDX1, NGN3, Glut2, insulin was monitored by reveres transcription polymerase chain reaction method. The differentiated clusters were stained with Dithizone(DTZ) which confirms the presence of insulin granules. The insulin challenge test(low and high glucose concentration in Krebs-Ringer HEPES buffer) was also used to evaluate the functional properties of differentiated clusters.RESULTS: WJ-MSCS were positive for mesenchymal surface markers(CD90, CD44, CD105), and negative for CD34 and CD133. The accumulation of lipid vacuoles and deposition of calcium mineral in cells were considered as adipogenic and osteogenic potential of WJ-MSCS. The cells also expressed the transcriptional factors such as Nanog and OCT4. During this three step differentiation, the WJ-MSCS morphology was gradually changed from spindle shaped cells in to epithelioid cells and eventually to three dimensional clusters. The clusters expressed PDX1, NGN3, Glut2, and insulin. The cells became bright red color when stained with DTZ and the insulin secretion was also confirmed. In glucose challenge test a significant increase in insulin secretion from 0.91 ± 0.04 μIu/mL(2.8 mmol/L glucose) to to 8.34 ± 0.45 μIu/mL(16.7 mmol/L glucose) was recorded(P < 0.05). The insulin secretion of undifferentiated WJ-MSCS was not changed in this challenge test.CONCLUSION: WJ-MSCs have the ability to differentiate in to islet-like cells in vitro. However, this process needs further optimization in order to generate efficient and functional IPCs. | Seideh Masoomeh Nekoei Negar Azarpira Ladan Sadeghi Sulmaz Kamalifar | 2015 | World Journal of Clinical Cases2015，3，7： | 3 |
| 2 | Web Services: Technologies and Benefits显示文摘 | Mohamad Ladan | 2010 | 通讯和计算机(中英文版)2010，7，6： | 2 |
| 3 | Redox-sensitive, PEG-shielded carboxymethyl PEI nanogels silencing MicroRNA-21, sensitizes resistant ovarian cancer cells to cisplatin显示文摘A series of branched polyethylenimine(PEI) modifications including PEGylation(PEG2 k-PEI) for steric shielding, redox-sensitive crosslinking for synthesis PEG2 k-PEI-ss nanogels and subsequent carboxymethylation(PEG2 k-CMPEI-ss) for modulation of the polymer pk a have been introduced for cellular delivery of Anti-mi R-21. The synthesis was characterized using 1 H NMR, FTIR, TNBS, potentiometric titration, particle size and ζ potential. Loading of Anti-mi R-21 at various N/P ratios was investigated by gel retardation, ethidium bromide dye exclusion, heparin sulfate competition and DNase I digestion experiments. The mi R-21 silencing was measured by stem-loop RT PCR in A2780 ovarian cancer cell lines whether it is sensitive to resistant to cisplatin. It has been shown that PEG2 k-CMPEI-ss was well suited for delivery of Anti-mi R-21 in terms of nucleic acid loading, preservation against extracellular matrix and nucleases and sequence-specific silencing of mi RNA-21 in vitro. Moreover, it has been demonstrated that pre-treating cells with Anti-mi R-21 loaded nanogels can sensitize them to cis-Pt even at non-toxic concentraions. The results indicate that PEG2 k-CMPEIss mediated micro RNA delivery can be considered as a novel strategy for ovarian cancer therapy. | Sanaz Javanmardi Ali Mohammad Tamaddon Mahmoud Reza Aghamaali Ladan Ghahramani Samira Sadat Abolmaali | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，1： | 2 |
| 4 | 传统园林美学在现代建筑景观中的运用研究显示文摘中国的古典园林是世界园林的重要组成部分,美学在园林中有不可或缺的影响,中国园林离不开中国古典哲学、古典美学的艺术体系带给园林的独特审美和构成手法。本文从传统园林美学为主要论述方面,探究传统园林美学在现代建筑景观中的运用研究。 | 邵琛寓 Ladan Abouali | 2020 | 建材与装饰2020，，21： | 2 |
| 5 | 尼日利亚豪萨族传统建筑材料及其营造研究显示文摘以尼日利亚北部豪萨族传统建筑为研究对象,通过实地调研、文献研究及对历史学家访谈等研究方法,从建筑材料及其建造技艺着眼,总结出豪萨族传统建筑在建筑形态、材料选取、低技术营造、生态性等方面具有的显著特征,提高人们对这一正在消失的独特传统建筑类型的认知。 | 张娟 朱庆玲 ZAHRADDEEN Ladan Yusuf | 2020 | 建筑技术2020，51，2： | 2 |
| 6 | Nonlinear microwave properties of YBaCuO thin films studied with the coplanar resonator technique显示文摘 | KOKABI H LADAN F R MARTINET C | 2002 | Physica C2002，，： | 1 |
| 7 | Evaluation of in vit-ro activity of essential oils against Trypanosoma brucei brucei and Trypanosoma evansi 显示文摘 | Habila N Agbaji AS Ladan Z | 2010 | J Parasitol Res2010，53，： | 1 |
| 8 | Web services testing approaches: A sur- vey and a classification显示文摘 | Ladan Mohamad I | 2010 | Communications in Computer and Information Science2010，88，2： | 1 |
| 9 | Long term performance of MBR for biological nitrogen removal from synthetic municipal wastewater 显示文摘 | Ladan Holakoo George Nakhla | 2007 | Chemosphere2007，，66： | 1 |
| 10 | Cataract surgery for the developing world显示文摘 | Geoffrey Tabin Michael Chen Ladan Espandar | 2008 | Current Opinion in Ophthalmology2008，，1： | 1 |
| 11 | Photodynamic inactivation of gram negative bacteria : problems and possible solutions 显示文摘 | Malik Z Ladan H Nitzan Y | 1990 | Photochemistry and Photobiology1990，，： | 1 |
| 12 | Long term performance of MBR for biological nitrogen removal from synthetic municipal wastewater 显示文摘 | Ladan Holakoo George Nakhla | 2007 | Chemosphere2007，，66： | 1 |
| 13 | Sensory modalities are not separate modalities: Plasticity and interactions 显示文摘 | Shimojo Shinsuke Shams Ladan | 2001 | Current Opinion in Neurobiology2001，11，4： | 1 |
| 14 | Visual illusion induced by sound 显示文摘 | Shams Ladan Kamitani Yukiyasu Shimojo Shinsuke | 2002 | Cognitive Brain Research2002，14，1： | 1 |
| 15 | Investigation of a Novel Degradable Ureteral Stent in a Porcine Model显示文摘 | Boris A. Hadaschik Ryan F. Paterson Ladan Fazli Kenneth W. Clinkscales Shalaby W. Shalaby Ben H. Chew | 2008 | The Journal of Urology2008，，3： | 1 |
| 16 | Highly Effi- cient Compact Rectenna for Wireless Energy Harves- ting Application显示文摘 | Ladan S Ghassemi N Ghiotto A | 2013 | IEEE Microwave Magazine2013，14，1： | 1 |
| 17 | Extraction of essential oils from the seeds of pomegranate using organic solvents and supercritical CO2 显示文摘 | HA JAR A KARAMATOLLAH R LADAN R | 2008 | J Am Oil Chem Soc2008，85，： | 1 |
| 18 | The bactericidal activity of a deuteroporphyrin-hemin mixture on Gram-positive bacteria显示文摘 | Malik Z Ladan L Nitzan Y | 1990 | J Photoehem Photobiol B : Biol1990，6，： | 1 |
| 19 | Establishment of the trimester-specific reference range for free thyroxine index 显示文摘 | Fereidoun A Ladan M Atieh A | 2013 | Thyroid2013，23，： | 1 |
| 20 | Cansleepandwakefulnessbedistinguishedinchildrenbycardiorespiratory and videotaperecordings- 显示文摘 | MorielliA LadanS DucharmeFM etal | 1996 | Chest1996，109，： | 1 |
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