|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis显示文摘 | Bin Zhang Chi Zhang Congge Liu Yanping Jing Yuan Wang Ling Jin Lei Yang Aigen Fu Jisen Shi Fugeng Zhao Wenzhi Lan Sheng Luan | 2018 | Molecular Plant2018，11，7： | 6 |
| 2 | Developing controllable hypermutable Clostridium cells through manipulating its methyl-directed mismatch repair system显示文摘Development of controllable hypermutable cells can greatly benefit understanding and harnessing microbial evolution.However,there have not been any similar sys-tems developed for Clostridium,an important bacterial genus.Here we report a novel two-step strategy for de-veloping controllable hypermutable cells of Clostridium acetobutylicum,an important and representative indus-trial strain.Firstly,the mutS/L operon essential for methyl-directed mismatch repair(MMR)activity was inactivated from the genome of C.acetobutylicum to generate hy-permutable cells with over 250-fold increased mutation rates.Secondly,a proofreading control system carrying an inducibly expressed mutS/L operon was constructed.The hypermutable cells and the proofreading control system were integrated to form a controllable hypermut-able system SMBMutC,of which the mutation rates can be regulated by the concentration of anhydrotetracycline(aTc).Duplication of the miniPthl-tetR module of the proof-reading control system further signifi cantly expanded the regulatory space of the mutation rates,demonstrating hypermutable Clostridium cells with controllable mutation rates are generated.The developed C.acetobutylicum strain SMBMutC2 showed higher survival capacities than the control strain facing butanol-stress,indicating greatly increased evolvability and adaptability of the controllable hypermutable cells under environmental challenges. | Guodong Luan Zhen Cai Fuyu Gong Hongjun Dong Zhao Lin Yanping Zhang Yin Li | 2013 | Protein & Cell2013，4，11： | 4 |
| 3 | Criteria of limiting temperature and parametric analysis of the large deflection behavior for fully restrained steel beams in fire显示文摘This paper mainly involve 3 parts:1) To apply the minimum principle of acceleration in dynamics of elastic-plastic continua at finite deformation to the statics problems,a computing model is presented for the restrained steel beams exposed to the fire.In this model,both effects of large deflection and thermal expansion deformation are taken into account,and the constitutive equations with the temperature effects are used.Then a dynamic finite difference(DFD) method is presented by using the dis-crete technique,which can be used in simulating the response of the steel beams at elevated temperature,and the large deflec-tion behavior and catenary action effects of the beams can be adequately expressed.The primary numerical results show that the method is valid and credible.Compared with other methods,this technique is very simple,and it can also be further devel-oped to simulate the behavior of steel beams subjected to the coupling loading of explosion and fire when both effects of strain rate and inertia are considered.2) By using this DFD method,detailed parametric analysis are presented so as to check the consistency of response results for several different formulas of thermal expansion deformation and retention factors of steel at elevated temperature,the influence of these parameters on the critical temperature is examined.3) Based on the analysis for the curves of temperature-generalized yield function comprised by the axial force and bending moment,both criteria to determine the limiting temperature(or failure temperature) of large deflection steel beams are presented more explicitly,that is,both lim-iting temperatures can be determined by if the catenary force begins to appear or arrives at the maximum value,respectively.It is shown by numerical results that both limiting temperatures are close to the both critical temperatures which are correspond-ing to the maximum deflections equal to span/20 and span/10,respectively.This conclusion may be helpful to make rational fire resisting design for the steel beams. | XI Feng LUAN YanPing | 2012 | Science China(Technological Sciences)2012，55，1： | 2 |
| 4 | Applications,of fluorescence lifetime imaging in clinical medicine显示文摘Fluorescence lifetime is not only associated with the molecular structure f fuorophores,but alsostrongly depends on the environment around them,which llows fuorescence lifetime imagingmicroscopy(FLIM)to be used as a tool for precise measurement of the cell or tisue microenvironment,This review introduces the basic principle of fuorescence lifetime imagingtechnology and its application in clinical medicine,including research and diagnosis of diseases inskin,brain,eyes,mouth,bone,blood vessels and cavity organs,and drug evaluation.As anoninvasive,nontoxic and nonionizing radiation technique,FLIM demonstrates excellent per-formance with high sensitivity and specificity,which allows to determine precise position of thelesion and,thus,has good potential for application in biomedical research and clinical diagnosis. | Zhanwen Wang Yanping Zheng Deqiang Zhao Ziwei Zhao Lixin Liu Artem Pliss Feiqi Zhu Jun Liu Junle Qu Ping Luan | 2018 | Journal of Innovative Optical Health Sciences2018，，1： | 1 |
| 5 | Analysis of Two-Grid Methods for Nonlinear Parabolic Equations by Expanded Mixed Finite Element Methods显示文摘In this paper,we present an efficient method of two-grid scheme for the approximation of two-dimensional nonlinear parabolic equations using an expanded mixed finite element method.We use two Newton iterations on the fine grid in our methods.Firstly,we solve an original nonlinear problem on the coarse nonlinear grid,then we use Newton iterations on the fine grid twice.The two-grid idea is from Xu's work[SIAM J.Numer.Anal.,33(1996),pp.1759–1777]on standard finite method.We also obtain the error estimates for the algorithms of the two-grid method.It is shown that the algorithm achieve asymptotically optimal approximation rate with the two-grid methods as long as the mesh sizes satisfy h=O(H^((4k+1)/(k+1))). | Yanping Chen Peng Luan Zuliang Lu | 2009 | Advances in Applied Mathematics and Mechanics2009，1，6： | 1 |
| 6 | Amplified Detection of Iron Ion Based on Plasmon Enhanced Fluorescence and Subsequently Fluorescence Quenching显示文摘A facile and rapid approach for detecting low concentration of iron ion(Fe3+) with improved sensitivity was developed on the basis of plasmon enhanced fluorescence and subsequently amplified fluorescence quenching.Au1Ag4@Si O2 nanoparticles were synthesized and dispersed into fluorescein isothiocyanate(FITC) solution. The fluorescence of the FITC solution was improved due to plasmon enhanced fluorescence. However, efficient fluorescence quenching of the FITC/Au1Ag4@Si O2 solution was subsequently achieved when Fe3+, with a concentration ranging from17 n M to 3.4 l M, was added into the FITC/Au1Ag4@Si O2 solution, whereas almost no fluorescence quenching was observed for pure FITC solution under the same condition. FITC/Au1Ag4@Si O2 solution shows a better sensitivity for detecting low concentration of Fe3+compared to pure FITC solution. The quantized limit of detection toward Fe3+was improved from 4.6 l M for pure FITC solution to 20 n M for FITC/Au1Ag4@Si O2 solution. | Lin Zhou Han Zhang Yanping Luan Si Cheng Li-Juan Fan | 2014 | Nano-Micro Letters2014，6，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员