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| 1 | Therapeutic effect of acupuncture and massage for shoulder-hand syndrome in hemiplegia patients:a clinical two-center randomized controlled trial显示文摘OBJECTIVE:To evaluate the therapeutic effects of acupuncture and massage for shoulder-hand syndrome in hemiplegia patients.METHODS:One hundred and twenty hemiplegia patients with stage I shoulder-hand syndrome were randomly divided into a group treated with standardized electric acupuncture and massage,and a group treated with rehabilitation therapy for 6 weeks.The primary indices evaluated were pain on passive movement of the shoulder using the numeric pain rating scale(NPRS),and the number of patients with shoulder-hand syndrome at Steinbrocker stage II or III after treatment.The secondary indices were Fugl-Meyer evaluation of functional movement of the upper limb and hand using the modified rankin scale(MRS).RESULTS:At post-treatment evaluation and a 12-week follow-up visit,NPRS score,number of patients with stage II or III shoulder-hand syndrome,and MRS score were all improved in the acupuncture-massage group compared with the rehabilitation group(P<0.05).On Fugl-Meyer evaluation,functional movement of the upper limb was also improved in the acupuncture-massage group compared with the rehabilitation group(P<0.05).CONCLUSION:Standardized acupuncture-massage therapy may have curative effects on shoulder-hand syndrome in hemiplegia patients. | Ning Li Fengwei Tian Chengwei Wang Pengming Yu Xi Zhou Qian Wen Xiulan Qiao Lu Huang | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 25 |
| 2 | Discriminated sgRNAs-Based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-Edited Cells显示文摘The development of CRISPR/Cas9-mediated base editing has made genomic modification more efficient. However, selection of genetically modified cells from millions of treated cells, especially plant cells, is still challenging. In this study, an efficient surrogate reporter system based on a defective hygromycin resistance gene was established in rice to enrich base-edited cells. After step-by-step optimization, the Discriminated sgRNAs-based SurroGate system (DisSUGs) was established by artificially differentiating the editing abilities of a wild-type single guide RNA (sgRNA) targeting the surrogate reporter gene and an enhanced sgRNA targeting endogenous sites. The DisSUGs enhanced the efficiency of screening base-edited cells by 3- to 5-fold for a PmCDA1-based cytosine-to-tyrosine base editor (PCBE), and 2.5- to 6.5-fold for an adenine base editor (ABE) at endogenous targets. These targets showed editing efficiencies of <25% in the conventional systems. The DisSUGs greatly enhanced the frequency of homozygous substitutions and expanded the activity window slightly for both a PCBE and an ABE. Analyses of the total number of single-nucleotide variants from whole-genome sequencing revealed that, compared with the no-enrichment PCBE strategy, the DisSUGs did not alter the frequency of genome-wide sgRNA-independent off-target mutations, but slightly increased the frequency of target-dependent off-target mutations. Collectively, the DisSUGs developed in this study greatly enhances the efficiency of screening plant base-edited cells and will be a useful system in future applications. | Wen Xu Yongxing Yang Ya Liu Guiting Kang Feipeng Wang Lu Li Xinxin Lv Si Zhao Shuang Yuan Jinling Song Ying Wu Feng Feng Xiaoqing He Chengwei Zhang Wei Song Jiuran Zhao Jinxiao Yang | 2020 | Molecular Plant2020，13，1： | 8 |
| 3 | Advanced multiple response surface method of sensitivity analysis for turbine blisk reliability with multi-physics coupling显示文摘To reasonably implement the reliability analysis and describe the significance of influencing parameters for the multi-failure modes of turbine blisk, advanced multiple response surface method(AMRSM) was proposed for multi-failure mode sensitivity analysis for reliability. The mathematical model of AMRSM was established and the basic principle of multi-failure mode sensitivity analysis for reliability with AMRSM was given. The important parameters of turbine blisk failures are obtained by the multi-failure mode sensitivity analysis of turbine blisk. Through the reliability sensitivity analyses of multiple failure modes(deformation, stress and strain) with the proposed method considering fluid–thermal–solid interaction, it is shown that the comprehensive reliability of turbine blisk is 0.9931 when the allowable deformation, stress and strain are3.7*10^(-3)m, 1.0023*10~9 Pa and 1.05*10^(-2)m/m, respectively; the main impact factors of turbine blisk failure are gas velocity, gas temperature and rotational speed. As demonstrated in the comparison of methods(Monte Carlo(MC) method, traditional response surface method(RSM), multiple response surface method(MRSM) and AMRSM), the proposed AMRSM improves computational efficiency with acceptable computational accuracy. The efforts of this study provide the AMRSM with high precision and efficiency for multi-failure mode reliability analysis, and offer a useful insight for the reliability optimization design of multi-failure mode structure. | Zhang Chunyi Song Lukai Fei Chengwei Lu Cheng Xie Yongmei | 2016 | Chinese Journal of Aeronautics2016，29，4： | 4 |
| 4 | Developing high-efficiency base editors by combining optimized synergistic core components with new types of nuclear localization signal peptide显示文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals. | Feipeng Wang Chengwei Zhang Wen Xu Shuang Yuan Jinling Song Lu Li Jiuran Zhao Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 3 |
| 5 | Whole-process design and experimental validation of landing gear lower drag stay with global/local linked driven optimization strategy显示文摘Landing gear lower drag stay is a key component which connects fuselage and landing gear and directly effects the safety and performance of aircraft takeoff and landing. To effectively design the lower drag stay and reduce the weight of landing gear, Global/local Linked Driven Optimization Strategy(GLDOS) was developed to conduct the overall process design of lower drag stay in respect of optimization thought. The whole-process optimization involves two stages of structural conceptual design and detailed design. In the structural conceptual design, the landing gear lower drag stay was globally topologically optimized by adopting multiple starting points algorithm. In the detailed design, the local size and shape of landing gear lower drag stay were globally optimized by the gradient optimization strategy. The GLDOS method adopts different optimization strategies for different optimization stages to acquire the optimum design effect. Through the experimental validation, the weight of the optimized lower dray stay with the developed GLDOS is reduced by 16.79% while keeping enough strength and stiffness, which satisfies the requirements of engineering design under the typical loading conditions. The proposed GLDOS is validated to be accurate and efficient in optimization scheme and design cycles. The efforts of this paper provide a whole-process optimization approach regarding different optimization technologies in different design phases, which is significant in reducing structural weight and enhance design tp wid 1 precision for complex structures in aircrafts. | Chengwei FEI Haotian LIU Zhengzheng ZHU Liqiang AN Shaolin LI Cheng LU | 2021 | Chinese Journal of Aeronautics2021，34，2： | 2 |
| 6 | Novel Kriging-Based Decomposed-Coordinated Approach for Estimating the Clearance Reliability of Assembled Structures显示文摘Turbine blisks are assembled using blades,disks and casings.They can endure complex loads at a high temperature,high pressure and high speed.The safe operation of assembled structures depends on the reliability of each component.Monte Carlo(MC)simulation is commonly used to analyze structural reliability,but this method needs to run thousands of computations.In order to assess the clearance reliability of assembled structures in an efficient and precise manner,the novel Kriging-based decomposed-coordinated(DC)(DCNK)approach is proposed by integrating the DC strategy,the Kriging model and the importance sampling-based Markov chain(MCIS)technique.In this method,the DC strategy is used to decompose a multi-objective problem into many single-objective problems.The relationships between these many single-objectives and the overall objective are then coordinated.The Kriging model is applied to establish the limit state functions of the single-objectives and multi-objective problems,while the MCIS method is used to assess the structural assembled clearance reliability.Moreover,a highly nonlinear complex compound function is first utilized to verify the DCNK model from a mathematical perspective.Then,the reliability of an aeroengine high-pressure turbine(HPT)blade-tip radial running clearance(BTRRC)is analyzed to validate the DCNK approach by considering thermo-structural interaction.The analytical results show that the reliability is 0.9976 when the allowance value of the BTTRC is 1.7650×10^(−3)m.Compared with different methodologies(including direct simulation,the classical Kriging model,and the weighted response surface method(WRSM)),the proposed method holds obvious advantages in computing time and precision,as well as simulation efficiency and precision.The efforts of this paper provide a useful approach to analyzing assembled clearance reliability and contribute to the development of structural reliability theory. | Da Teng Yunwen Feng Cheng Lu Chengwei Fei Jiaqi Liu Xiaofeng Xue | 2021 | Computer Modeling in Engineering & Sciences2021，，11： | 2 |
| 7 | The Pumilio RNA-binding protein APUM24 regulates seed maturation by fine-tuning the BPM-WRI1 module in Arabidopsis显示文摘Pumilio RNAbindingproteinsparticipateinmes-senger RNA(mRNA)degradation and t ranslational repression,but their roles in plant development are largely unclear.Here,we show that Arabidopsis PUMILIO PROTEIN24(APUM24),an atypical Pumiliohomology domaincontaining protein,plays an im-portant part in regulating seed maturation,a major stage of plant development.APUM24 is strongly expressed in maturing seeds.Reducing APUM24 expression resulted in abnormal seed maturation,wrinkled seeds,and lower seed oil contents,and APUM24 knockdown resulted in lower levels of WRINKLED 1(WRI1),a key transcription factor con-trolling seed oil accumulation,and lower expression of WRI1 target genes.APUM24 reduces the mRNA stability of BTB/POZMATH(BPM)family genes,thus decreasing BPM protein levels.BPM is responsible for the 26S proteasomemediated degradation of WRI1 and has important functions in plant growth and development.The 3′untranslated regions of BPM family genes contain putative Pumilio response elements(PREs),which are bound by APUM24.Re-duced BPM or increased WRI1 expression rescued the decient seed maturation of apum242 knock-down mutants,and APUM24 overexpression re-sulted in increased seed size and weight.Therefore,APUM24 is crucial to seed maturation through its action as a positive regulatornetuning the BPMWRI1 module,making APUM24 apromising target for breeding strategies to increase crop yields. | Ruihua Huang Mengling Liu Guanping Gong Pingzhi Wu Barunava Patra Ling Yuan Hongting Qin Xiaoxu Wang Guohe Wang Huimei Liao Lu Gao Chengwei Yang Hongqing Li Shengchun Zhang | 2021 | Journal of Integrative Plant Biology2021，63，7： | 2 |
| 8 | Dynamic parametric modeling-based model updating strategy of aeroengine casings显示文摘For accurate Finite Element(FE)modeling for the structural dynamics of aeroengine casings,Parametric Modeling-based Model Updating Strategy(PM-MUS)is proposed based on efficient FE parametric modeling and model updating techniques regarding uncorrelated/correlated mode shapes.Casings structure is parametrically modeled by simplifying initial structural FE model and equivalently simulating mechanical characteristics.Uncorrelated modes between FE model and experiment are reasonably handled by adopting an objective function to recognize correct correlated modes pairs.The parametrized FE model is updated to effectively describe structural dynamic characteristics in respect of testing data.The model updating technology is firstly validated by the detailed FE model updating of one fixed–fixed beam structure in light of correlated/uncorrelated mode shapes and measured mode data.The PM-MUS is applied to the FE parametrized model updating of an aeroengine stator system(casings)which is constructed by the proposed parametric modeling approach.As revealed in this study,(A)the updated models by the proposed updating strategy and dynamic test data is accurate,and(B)the uncorrelated modes like close modes can be effectively handled and precisely identify the FE model mode associated the corresponding experimental mode,and(C)parametric modeling can enhance the dynamic modeling updating of complex structure in the accuracy of mode matching.The efforts of this study provide an efficient dynamic model updating strategy(PM-MUS)for aeroengine casings by parametric modeling and experimental test data regarding uncorrelated modes. | Chengwei FEI Haotian LIU Shaolin LI Huan LI Liqiang AN Cheng LU | 2021 | Chinese Journal of Aeronautics2021，34，12： | 2 |
| 9 | Validation of the simplified criteria for diagnosis of autoimmune hepatitis in Chinese patients显示文摘 | Dekai Qiu Qixia Wang Hui Wang Qing Xie Guoqing Zang Hong Jiang Chuantao Tu Jinsheng Guo Shuncai Zhang Jianshe Wang Yi Lu Ying Han Lei Shen Xiaoyu Chen Xiqi Hu Xiaojin Wang Chengwei Chen Qingchun Fu Xiong Ma | 2010 | Journal of Hepatology2010，，： | 2 |
| 10 | Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing显示文摘Base editing, as an expanded clustered regularly interspaced short palindromic repeats(CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus(SaCas9), is important in genome editing because it can edit sites with HHHAAT protospacer adjacent motif(PAM) that the canonical Streptococcus pyogenes Cas9(SpCas9) or its variants(e.g. xCas9, Cas9-NG) cannot. However, several technical parameters of SaKKH involved base editors have not been well defined and this uncertainty limits their application. We developed an effective multiplex cytosine base editor(SaKKHn-pBE) and showed that it recognized NNARRT, NNCRRT, NNGRGT, and NNTRGT PAMs. Based on 27 targets tested, we defined technical parameters of SaKKHn-pBE including the editing window, the preferred sequence context, and the mutation type. The editing efficiency was further improved by modification of the SaKKH sgRNA. These advances can be applied in future research and molecular breeding in rice and other plants. | Chengwei Zhang Feipeng Wang Si Zhao Guiting Kang Jinling Song Lu Li Jinxiao Yang | 2020 | The Crop Journal2020，8，3： | 1 |
| 11 | Generation of enhanced stability of SnO/In(OH)_(3)/InP for photocatalytic water splitting by SnO protection layer1 Generation of enhanced stability of SnO/In(OH)_(3)/InP for photocatalytic water splitting by SnO protection layer1显示文摘InP shows a very high efficiency for solar light to electricity conversion in solar cell and may present an expectation property in photocatalytic hydrogen evolution.However,it suffers serious corrosion in water dispersion.In this paper,it is demonstrated that the stability and activity of the InP-based catalyst are effectively enhanced by applying an anti-corrosion SnO layer and In(OH)_(3)transition layer,which reduces the crystal mismatch between SnO and InP and increases charge transfer.The obtained Pt/SnO/In(OH)_(3)/InP exhibits a hydrogen production rate of 144.42μmol/g in_(3)h under visible light illumination in multi-cycle tests without remarkable decay,12_(3)times higher than that of naked In(OH)_(3)/InP without any electron donor under visible irradiation. | Jiali DONG Xuqiang ZHANG Gongxuan LU Chengwei WANG | 2021 | Frontiers in Energy2021，15，3： | 1 |
| 12 | Efficacy and safety of entecavir compared to lamivudine in nucleoside-na?ve patients with chronic hepatitis B: a randomized double-blind trial in China显示文摘 | Guangbi Yao Chengwei Chen Weilun Lu Hong Ren Deming Tan Yuming Wang Daozheng Xu Zhengrong Jiang Jessica Liu Dong Xu Laurie MacDonald | 2007 | Hepatology International2007，，3： | 1 |
| 13 | Influence of Heating Rate on Double Reversible Transformation in CuZnAlMnNi Shape Memory Alloy显示文摘The influence of heating rate on double reversible transformation in CuZnAlMnNi shape memory alloy was investigated by differential scanning calorimetry. It was found that rapid heating inhibits X-M transformation but is fa- vorable to the reverse medensite transformation, giving rise to the approach of the two transformation peaks. With the decrease of heating rate, the two transformation peaks separate gradually. | Yujun Bai, Chengwei Lu, Xiangang Xu, Guili Geng, Longwei Yin (Mechanical Department, Shandong University of Science and Technology, Jinan 250031, China Institute of Materials Science and Engineering, Shandong University, Jinan 250061, China) | 2001 | Rare Metals2001，20，2： | 1 |
| 14 | Virologic, serologic, and biochemical outcomes through 2?years of treatment with entecavir and lamivudine in nucleoside-na?ve Chinese patients with chronic hepatitis B: a randomized, multicenter study显示文摘 | Guangbi Yao ChengWei Chen WeiLun Lu Hong Ren DeMing Tan YuMing Wang DaoZheng Xu Jessica Liu Dong Xu Cyril Llamoso | 2008 | Hepatology International2008，，4： | 1 |
| 15 | Developing an efficient and visible prime editing system to restore tobacco 8-hydroxy-copalyl diphosphate gene for labdane diterpene Z-abienol biosynthesis显示文摘Prime editing(PE)is a versatile CRISPR-Cas based precise genome-editing platform widely used to introduce a range of possible base conversions in various organisms.However,no PE systems have been shown to induce heritable mutations in tobacco,nor in any other dicot.In this study,we generated an efficient PE system in tobacco that not only introduced heritable mutations,but also enabled anthocyanin-based reporter selection of transgene-free T_(1) plants.This system was used to confer Zabienol biosynthesis in the allotetraploid tobacco cultivar HHDJY by restoring a G>T conversion in the NtCPS2 gene.High levels of Z-abienol were detected in the leaves of homozygous T_(1) plants at two weeks after topping.This study describes an advance in PE systems and expands genome-editing toolbox in tobacco,even in dicots,for use in basic research and molecular breeding.And restoring biosynthesis of Z-abienol in tobacco might provide an efficient way to obtain Z-abienol in plants. | Jianduo Zhang Lu Zhang Chengwei Zhang Yongxing Yang Huayin Liu Lu Li Shengxue Zhang Xianggan Li Xinxiang Liu Ya Liu Jin Wang Guangyu Yang Qingyou Xia Weiguang Wang Jinxiao Yang | 2023 | Science China(Life Sciences)2023，66，12： | 1 |
| 16 | Coronary artery stenting in patients with atrial fibrillation after triple antithrombotic contrast two antithrombotic therapy efficacy and safety analysis of 显示文摘 | Lu Wenbin Chen Lijuan Ju Chengwei | 2015 | China Journal of interventional cardiology2015，15，02： | 1 |
| 17 | Lockdown-induced Urban Aerosol Change over Changchun, China During COVID-19 Outbreak with Polarization LiDAR显示文摘Depending on various government policies,COVID-19(Corona Virus Disease-19) lockdowns have had diverse impacts on global aerosol concentrations.In 2022,Changchun a provincial capital city in Northeast China,suffered a severe COVID-19 outbreak and implemented a very strict lockdown that lasted for nearly two months.Using ground-based polarization Light Detection and Ranging(LiDAR),we detected real-time aerosol profile parameters(EC,extinction coefficient;DR,depolarization ratio;AOD,aerosol optical depth),as well as air-quality and meteorological indexes from 1 March to 30 April in 2021 and 2022 to quantify the effects of lockdown on aerosol concentrations.The period in 2022 was divided into three stages:pre-lockdown(1-10 March),strict lockdown(11 March to 10 April),and partial lockdown(11-30 April).The results showed that,during the strict lockdown period,compared with the pre-lockdown period,there were substantial reductions in aerosol parameters(EC and AOD),and this was consistent with the concentrations of the atmospheric pollutants PM_(2.5)(particulate matter with an aerodynamic diameter ≤2.5 μm) and PM_(2.5)(particulate matter with an aerodynamic diameter ≤10 μm),and the Oconcentration increased by 8.3%.During the strict lockdown,the values of EC within0-1 km and AOD decreased by 16.0% and 11.2%,respectively,as compared to the corresponding period in 2021.Lockdown reduced the conventional and organized emissions of air pollutants,and it clearly delayed the time of seasonal emissions from agricultural burning;however,it did not decrease the number of farmland fire points.Considering meteorological factors and eliminating the influence of wind-blown dust events,the results showed that reductions from conventional organized emission sources during the strict lockdown contributed to a 30% air-quality improvement and a 22% reduction in near-surface extinction(0-2 km).Aerosols produced by urban epidemic prevention and disinfection can also be identified using the EC.Regarding seasonal sources of agricultural straw burning,the concentrated burning induced by the epidemic led to the occurrence of heavy pollution from increased amounts of atmospheric aerosols,with a contribution rate of 62%.These results indicate that there is great potential to further improve air quality in the local area,and suggest that the comprehensive use of straw accompanied by reasonable planned burning is the best way to achieve this. | CHEN Weiwei DUANMU Lingjian QIN Yang YANG Hongwu FU Jing LU Chengwei FENG Wei GUO Li | 2022 | Chinese Geographical Science2022，32，5： | 0 |
| 18 | Discovery of a potent and highly selective inhibitor of SIRT6 against pancreatic cancer metastasis in vivo显示文摘Pancreatic cancer,one of the most aggressive malignancies,has no effective treatment due to the lack of targets and drugs related to tumour metastasis.SIRT6 can promote the migration of pancreatic cancer and could be a potential target for antimetastasis of pancreatic cancer.However,highly selective and potency SIRT6 inhibitor that can be used in vivo is yet to be discovered.Here,we developed a noveSIRT6 allosteric inhibitor,compound 11e,with maximal inhibitory potency and an IC_(50) value of 0.98±0.13μmol/L.Moreover,compound 11e exhibited significant selectivity against other histone deacetylases(HADC1-11 and SIRT1-3)at concentrations up to 100μmol/L.The allosteric site and the molecular mechanism of inhibition were extensively elucidated by cocrystal complex structure and dynamic structural analyses.Importantly,we confirmed the antimetastatic function of such inhibitors in four pancreatic cancer cell lines as well as in two mouse models of pancreatic cancer liver metastasis.To our knowledge,this is the first study to reveal the in vivo effects of SIRT6 inhibitors on liver metastatic pancreatic cancer.It not only provides a promising lead compound for subsequent inhibitor developmentargeting SIRT6 but also provides a potential approach to address the challenge of metastasis in pancreatic cancer. | Xinyuan Xu Qian Zhang Xufeng Wang Jing Jin Chengwei Wu Li Feng Xiuyan Yang Mingzhu Zhao Yingyi Chen Shaoyong Lu Zhen Zheng Xiaobing Lan Yi Wang Yan Zheng Xuefeng Lu Qiufen Zhang Jian Zhang | 2024 | Acta Pharmaceutica Sinica B2024，14，3： | 0 |
| 19 | One-pot Three-component Reaction for Efficient and Facile Synthesis of γ-Nitrocarbonyl Compounds Under Solvent-free Conditions显示文摘 | LU Chengwei LIU Yanhang ZHOU Xiaoxia WANG Jiajing | 2015 | Chemical Research in Chinese Universities2015，31，2： | 0 |
| 20 | Rapid Molecular Identification of Tribolium destructor显示文摘In this study,a rapid molecular identification method of Tribolium destructor was established with PCR and PCR-RFLP( polymerase chain reaction-restriction fragment length polymorphism) technology. According to the results,( 1) with PCR method,specific primers were designed based on COI gene of T. destructor for PCR amplification,and electrophoresis detection confirmed that PCR method could be used to rapidly and accurately identify T. destructor;( 2) with PCR-RFLP method,two pairs of degenerate primers were used to amplify COI gene of Tribolium species,PCR products were digested with HindIII and detected by electrophoresis,results indicated that PCR-RFLP method could also be used for rapid identification of T. destructor in quarantine practice. | Chengwei ZHANG Liuping XU Miao LU Xiaosong LIANG Jian LI | 2014 | Agricultural Biotechnology2014，3，4： | 0 |
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