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| 1 | An AIEgens and exonuclease Ⅲ aided quadratic amplification assay for detecting and cellular imaging of telomerase activity显示文摘Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis.In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens modified probe(TPE-Py-DNA) as a fluorescence reporter and exonuclease Ⅲ(Exo Ⅲ) as a signal amplifier.With the aid of telomerase, repeat units(TTAGGG)n are extended from the end of template substrate oligonucleotides(TS primer) that form duplex DNAs with TPE-Py-DNA. Then, Exo Ⅲ catalyzes the digestion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal.The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally,we obtain the target-to-signal amplification ratio of 1:N^2. This strategy exhibits good performance for detecting clinical urine samples(distinguishing 15 cancer patients' samples from 8 healthy ones) and checking intracellular telomerase activity(differentiating cell lines including He La, MDA-MB-231,MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug), which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer. | Xuehong Min Lei Xia Yuan Zhuang Xudong Wang Jie Du Xiaojin Zhang Xiaoding Lou Fan Xia | 2017 | Science Bulletin2017，62，14： | 5 |
| 2 | A highly sensitive DNA-AIEgen-based "turn-on" fluorescence chemosensor for amplification analysis of Hg^2+ ions in real samples and living cells显示文摘Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg^(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg^(2+) in real water samples and mapping of intracellular Hg^(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps. | Xiaowen OU Xiaoding Lou Fan Xia | 2017 | Science China Chemistry2017，60，5： | 4 |
| 3 | DNA hybridization chain reaction and DNA supersandwich self-assembly for ultrasensitive detection显示文摘The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and ions.DNA hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the sensors.In this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and SSA.This review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions. | Nannan Liu Fujian Huang Xiaoding Lou Fan Xia | 2017 | Science China Chemistry2017，60，3： | 4 |
| 4 | Rational Fabrication and Biomedical Application of Biomolecule-Conjugated AIEgens through Click Reaction显示文摘Recently,the issue of cancer has attracted extensive attention.Early diagnosis and timely therapy are important for cancer treatment.And lots of advanced fluorescent probes have been applied to cancer theranostics.However,the further development of these probes is limited by the disadvantages of poor targeting,weak sensitivity and photobleaching.Fortunately,the emergence of biomolecule-conjugated fluorescent probes with aggregationinduced emission properties has taken innovative impetus to the cancer theranostics.This review summarizes the rational fabrication and biomedical applications of biomolecule-conjugated A1E luminogens(AIEgens)based on'click reaction'over the past decade.In the meantime,the challenges of biomolecule-conjugated AIEgens in the field of biomedicine are also discussed. | Qiming Yuan Yong Cheng Xiaoding Lou Fan Xia | 2019 | Chinese Journal of Chemistry2019，37，10： | 3 |
| 5 | DDTC-Na-based colorimetric chemosensor for the sensing of cyanide in water显示文摘Sodium diethyldithiocarbamate (DDTC-Na) was demonstrated to be a new colorimetric cyanide chemosensor by utilizing an indirect trick. First, some copper ions were added to the colorless aque- ous solution of DDTC-Na. Then, the resultant brown solution was studied upon the addition of different anions, including Cl-, I-, IO3-, SO42-, NO-2, Br-, H2PO4-, F-, SCN-, HSO-4, ClO-4 and CN-. It was observed by naked eyes that the brown solution changed to colorless immediately after the addition of the trace cyanide, but there were no changes towards other anions, making DDTC-Na a good selective cyanide chemosensor in pure water. | LOU XiaoDing LI Zhen QIN JinGui | 2009 | Science China Chemistry2009，52，6： | 2 |
| 6 | Recent advances in optical-based and force-based single nucleic acid imaging显示文摘The capability to image, as well as control and manipulate single molecules such as nucleic acids(DNA or RNA) can greatly enrich our knowledge of the roles of individual biomolecules in cellular processes and their behavior in native environments. Here we summarize the recent advances of single nucleic acid imaging based on optical observation and force manipulation. We start by discussing the superiority of single molecule image, the central roles nucleic acids play in biosystems, and the significance of single molecule image towards nucleic acids. We then list a series of representative examples in brief to illustrate how nucleic acid of various morphologies has been imaged from different aspects, and what can be learned from such characterizations. Finally,concluding remarks on parts of which should be improved and outlook are outlined. | Shenshan Zhan Xiaoding Lou Fan Xia | 2017 | Science China Chemistry2017，60，10： | 2 |
| 7 | Car-following behavior modeling driven by small data sets based on mnemonic extreme gradient boosting framework显示文摘Dear editor,In recent years,data-driven car-following models have been developed based on their ability to drill down to information in driving data and their flexibility.According to a study by Fleming et al.[1],the main limitation of existing methods is an insufficient amount of natural driving data.In addition,there are many situations during actual driving processes,such as those under extreme conditions[2]and in the early stages of car-following behavior modeling.In these cases,sparse data learning algorithms are indispensable. | Baichuan LOU Yufang LI Xiaoding LU Zhe XU | 2022 | Science China(Information Sciences)2022，65，6： | 2 |
| 8 | A selective glutathione probe based on AlE fluorogen and its application in enzymatic activity assay显示文摘 | LOU Xiaoding HONG Yuning CHEN Sijie | 2014 | Scientific reports2014，4，6175： | 1 |
| 9 | Aggregation-induced emission luminogens reveal cell cycle-dependent telomerase activity in cancer cells显示文摘Telomerase acts as an important biomarker for tumor identification, and synthesizes telomeric repeats at the end of chromosome telomeres during the replicative phase of the cell cycle;thus, the expression level of telomerase changes as the cell cycle progresses. TERT m RNA expression and telomerase activity were significantly increased in over 80% of human cancers from tissue specimens. Although many efforts have been made in detecting the activity of TERT m RNA and active telomerase, the heterogeneous behavior of the cell cycle was overlooked, which might affect the accuracy of the detection results. Herein, the AIEgen-based biosensing systems of Py TPA-DNA and Silole-R were developed to detect the cellular level of TERT m RNA and telomerase in different cell cycles. As a result, the fluorescence signal of cancer cells gradually increased from G0/G1, G1/S to S phase. In contrast, both cancer cells arrested at G2/M phase and normal cells exhibited negligible fluorescence intensities. Compared to normal tissues, malignant tumor samples demonstrated a significant turn-on fluorescence signal. Furthermore, the transcriptomics profiling revealed that tumor biomarkers changed as the cell cycle progressed and biomarkers of CA9, TK1 and EGFR were more abundantly expressed at early S stage. In this vein, our study presented advanced biosensing tools for more accurate analysis of the cell-cycle-dependent activity of TERT m RNA and active telomerase in clinical tissue samples. | Xia Wu Jun Wu Jun Dai Biao Chen Zhe Chen Shixuan Wang Feng Wu Xiaoding Lou Fan Xia | 2021 | National Science Review2021，8，6： | 1 |
| 10 | Nanopores/Nanochannels Based on Electrical and Optical Dual Signal Response for Application in Biological Detection显示文摘Cancers and chronic diseases have always been global health problems. The occurrence and development of such diseases are closely related to the abnormalities of proteins, nucleic acids, ions or small molecules in the body. Nowadays, nanopores/nanochannels have emerged as a powerful platform for detecting these biomolecules based on the electrical signal variation caused by biomolecules passing. However, detection relied on the electrical signal easily suffered from the clogging defects, low throughput, and strong background signals. Fortunately, the emergence of designing nanopores/nanochannels based on electrical and optical dual signal response has brought innovative impetus to biological detection, which can also identify the chemical compositions and conformations of the biomolecules. In this review, we summarize the reasonable preparation of nanopores/nanochannels with electrical and optical dual signal response and their application in biological detection. According to different biomolecules, we divide the targets into four types, including nucleic acids, small molecules, ions and proteins. In each section, the design of representative examples and the principle of dual signal generation are introduced and discussed. Finally, the prospects and challenges of nanopores/nanochannels based on electrical and optical dual signal response are also discussed. | Guangwen Lu Niya Lin Zhaojun Chen Wenlian Jiang Jing-Jing Hu Fan Xia Xiaoding Lou | 2023 | Chinese Journal of Chemistry2023，41，11： | 1 |
| 11 | Red AIE conjugated polyelectrolytes for long-term tracing and image-guided photodynamic therapy of tumors显示文摘Robust photosensitizers with strong red/NIR fluorescence, efficient reactive oxygen species(ROS) generation and high photostability are highly desired for photodynamic therapy(PDT). Herein, three novel red conjugated polyelectrolytes(CPEs) with tetraphenylethene and 2,1,3-benzothiadiazole on the main chains and triphenylphosphonium on the side chains are developed.These CPEs display apparent aggregation-induced emission feature and high fluorescence quantum yields in the aggregated state. They can target lysosome in He La cells for fluorescence bioimaging. By virtue of the good retention effect and high photostability, these CPEs show ultralong-term tracing performance of subcutaneous tumors, and the tumor site can still be visualized for 20 days after injection. Owing to their good biocompatibility and strong ROS generation ability, the image-guided PDT based on these CPEs can effectively inhibit the growth of subcutaneous tumor and significantly prolong the survival of tumor bearing mice. The H&E and IHC staining reveal that the PDT of these CPEs depress the proliferation of tumor cells, and promote apoptosis and necrosis process. These new CPEs may be employed both as fluorescent probes for in vitro and in vivo long-term tracing and as photosensitizers for image-guided PDT of tumors. | Hongming Yao Jun Dai Zeyan Zhuang Jinya Yao Zixuan Wu Shixuan Wang Fan Xia Jian Zhou Xiaoding Lou Zujin Zhao | 2020 | Science China Chemistry2020，63，12： | 1 |
| 12 | Recent Development of DNA-modified AIEgen Probes for Biomedical Application显示文摘A variety of DNA-based probes are utilized for the detections of multiple analytes and DNA nanotechnology has been thriving for recent decades and achieving numerous nanostructures,mainly focusing on DNA morphology modulation and multifunctional systems engineered into to the complicated works.Among the numerous detections,fluorescence method is a non-invasive,highly selective and sensitive means for varieties of applications,but their emissions are often compromised by the aggregation-caused quenching(ACQ)effect,which weakens their applications.The aggregation induced emission luminogens(AIEgens)are created with non emissive or weakly emissive in a low concentration but emit strong fluorescence in a high concentration with aggregated states.Herein,numerous functionalized AIEgens have been emerged and used for detection and imaging and DNA-modified AIEgen probes are introduced.In this vein,here we report the progress on DNA-modified AIEgen probes in recent years and highlight their conjugation strategies including covalent bonding,electrostatic interaction and their applications of biosensing.Moreover,multiple DNA strands are needed to introduce into the DNA-modified AIEgen probes for more purposes.At the end,some challenges are mentioned to discuss the new trend of DNA-modified AIEgen probes. | HU Qinyu WU Jun CHEN Lulu LOU Xiaoding XIA Fan | 2021 | Chemical Research in Chinese Universities2021，37，1： | 0 |
| 13 | Multifunctional aggregates for precise cellular analysis显示文摘Precisely analyzing the target materials in living cells can reveal the essence and mystery of life at a deeper level,which will provide reliable theoretical basis for the occurrence,development,treatment and prognosis of major diseases.However,because living cells are in the dynamic process of metabolism,there are several challenges existed in accurate analysis,including subcellular compartment heterogeneity,plasma membrane interface barrier,and cell cycle regulation.In this regard,our group has designed and synthesized a series of multifunctional aggregates by mainly integrating the peptide elements,nucleic acid elements and aggregation elements to overcome the barriers.This article summarizes the latest developments of multifunctional aggregates for precise cell analysis by our group,and systematically introduces them according to different design concepts and targeting dimensions,such as space,efficiency as well as time.We hope this work could contribute to analyzing the biomarkers in cells through constructing the multifunctional aggregates,understanding the operation mechanism of cells,finally inspiring technology breakthroughs in biomedical fields. | Jing-Jing Hu Xiaoqi Dong Wenlian Jiang Fan Xia Xiaoding Lou | 2021 | Science China Chemistry2021，64，11： | 0 |
| 14 | One-dimensional and two-dimensional nanomaterials for the detection of multiple biomolecules显示文摘The complexity of biological samples determines that the detection of a single biomolecule is unable to satisfy actual needs. Moreover, the 'false positives' results caused by a single biomolecule detections easily leads to erroneous clinical diagnosis and treatment. Thus, it is important for the homogenous quantification of multiple biomolecules in not only basic research but also practical application. As a consequent, a large number of literatures have been exploited to monitor multiple biomolecules in homogenous solution, enabling facilitating the development of the disease diagnosis, treatment as well as drug discovery. One-dimensional nanomaterials and two-dimensional nanomaterials have special physical and chemical properties, such as good electrochemical properties, stable structure, large specific surface area, and biocompatibility, which are widely used in electrochemical and fluorescent detection of biomolecules. This tutorial review highlights the recent development for the detection of multiple biomolecules by using nanomaterials including one-dimensional materials(1DMs) as well as twodimensional materials(2DMs). | Quan Wang Xudong Wang Min Xu Xiaoding Lou Fan Xia | 2019 | Chinese Chemical Letters2019，30，9： | 0 |
| 15 | Peptide-Conjugated AggregationInduced Emission Fluorogen:Precise and Firm Cell Membrane Labeling by Multiple Weak Interactions显示文摘The cell membrane is a vital barrier that protects the cell from external damage and is involved in many biochemical processes.Thus,it is of great significance to label the cell membrane to explore its function.However,due to its complex and dynamic nature,precise and firm cell membrane labeling simultaneously is still a challenge.Herein,we report the fabrication of a peptide-conjugated aggregationinduced emission fluorogen(AIEgen),RTP,consisting of three main components:(1)An integrin-targeting peptide(RGD,R),which could bind specifically to integrinαvβ3 on cell membranes through ligand–receptor interaction.(2)An AIE-active tetraphenylethene derivative(T-MY,T)for fluorescent imaging.(3)Palmitic acid-modified peptide(Pal-RRRR,P),in which Pal isinserted into the lipid on the cellmembrane by hydrophobic interaction,and RRRR interacted with the negatively charged cell membrane components(proteins and lipids)through electrostatic forces.RTP could precisely label tumor cells with high integrinαvβ3 expression andfirmly trace the cellmembrane for up to 4 h;it also has a strong resistance to photobleaching.Moreover,RTP achieved in vivo tumor-specific imaging via cell membrane labeling.Thereby,utilizing multiple weak interactions between the fluorescent probe and the cell membrane provided a new strategy for precise and firm imaging of the cell membrane simultaneously. | Juliang Yang Jing-Jing Hu Jiaming Wei Jun Dai Rui Liu Fan Xia Xiaoding Lou | 2022 | CCS Chemistry2022，4，2： | 0 |
| 16 | Particle-Wave Dualism in Nanoconfined Space:Ultrafast Substance Flow显示文摘Many researchers,however,found that(1)the flow of both liquid and gas through nanoscale pores is one to even seven orders of magnitude faster than that would be predicted from the classic Newton’s mechanic theories,such as the Hagen-Poiseuille equation,the Bernoulli’s principle,the Knudsen theory;(2)the seeming contradiction that K+channels conduct K+ions at maximal throughput rates while not permeating slightly smaller Na+ions,which have perplexed scientists for decades.Herein we propose a possible explanation for the above phenomena based on the Wave-Particle Dualism.The quantum effect on ultrafast flow could possibly provide a new perspective for studying the nature of the ion and molecule channels,which are the backbones for the biology,and possibly promote the development of new methods for energy conversion,desalination of sea water and even information systems. | GAO Pengcheng MA Qun LIU Rui LOU Xiaoding HUANG Yu ZHANG Baocheng XIA Fan | 2022 | Chemical Research in Chinese Universities2022，38，4： | 0 |
| 17 | Recent advances in stimuli-responsive theranostic systems with aggregation-induced emission characteristics显示文摘Theranostic systems by integrating the tumor imaging and tumor therapeutic capabilities into one platform have attracted numerous attentions from worldwide researchers.Despite the great developments,their clinical application is still in the nascent stage,owing to the unsatisfied imaging quality and limited therapeutic efficacy.Fortunately,the emerging of aggregation-induced emission(AIE)molecules with unique fluorescence property offers an opportunity to solve the imaging problem.Besides,further utilizing the tumor microenvironments and external triggers to design the stimuli-responsive imaging-guided therapy could enhance the therapeutic efficacy and reduce the side effects.In this review,the advancements in stimuli-responsive theranostic systems with AIE characteristics are summarized.Theranostic systems are first classified according to their treatment modes,and then subdivided based on various stimuli,including pH,redox,enzyme,and light.In each section,the design strategies and application examples are introduced.At last,the current state of the art,limitations,as well as prospects are also discussed. | Jing-Jing Hu Wenlian Jiang Lizhen Yuan Chong Duan Qiming Yuan Zi Long Xiaoding Lou Fan Xia | 2021 | Aggregate2021，2，1： | 0 |
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