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| 1 | Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone显示文摘Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody(mAb) probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid(LTA). Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PET imaging in an in vivo mouse model of prosthetic joint infection(PJI). In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography(PET) with [^(18)F]FDG and [^(18)F]Na F as well as X-ray computed tomography(CT), before being evaluated with the zirconium-89-labeled antibody specific for LTA([^(89)Zr]SAC55).The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater(P < 0.05) uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^(89)Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI. | Julie E.Pickett John M.Thompson Agnieszka Sadowska Christine Tkaczyk Bret R.Sellman Andrea Minola Davide Corti Antonio Lanzavecchia Lloyd S.Miller Daniel LJ Thorek | 2018 | Bone Research2018，6，2： | 3 |
| 2 | Childhood obesity and risk of the adult metabolic syndrome:a systematic review显示文摘 | Lloyd LJ Langley-Evans SC McMullen S | | 0，，： | 1 |
| 3 | The influence of small fibre muscle mechanoreceptors on the cardiac vagus in humans显示文摘 | Gladwell VF Fletcher J Patel N Elvidge LJ Lloyd D Chowdhary S | | 0，，2： | 1 |
| 4 | Childhood obe- sity and adult cardiovascular disease risk :A systematic review 显示文摘 | LLOYD LJ LANGLEY-EVANS SC MCMULLEN S | 2010 | Int J Obes (Lond)2010，34，1： | 1 |
| 5 | Suppression of acute proinflammatory cytokine and chemokine upregulation by post-injury administration of a novel small molecule improves long-term neurologic outcome in a mouse model of traumatic brain injury显示文摘 | Lloyd E Somera-Molina K Van-Eldik LJ | 2008 | J Neuroinflammation2008，5，1： | 1 |
| 6 | Transanal en- doscopic microsurgery-lessons from a single UK centre series显示文摘 | Lloyd GM Sutton CD Marshall LJ | 2002 | Colorectal Dis2002，4，6： | 1 |
| 7 | Immunological studies before and during interferon therapy in chronic HBV infection:identification of factors predicting response显示文摘 | Scully LJ Brown D Lloyd C | 1990 | Hepatology1990，12，5： | 1 |
| 8 | Suppression of acute proinflammatory cytokine and chemokine upregulation by post-injury administration of a novel small molecule improves long-term neurologic outcome in a mouse model of traumatic brain injury显示文摘 | Lloyd E Somera-Molina K Van Eldik LJ | 2008 | J Neuroinflammation2008，5，1： | 1 |
| 9 | Childnood obesity and risk of the adult metabolic syndrome:A systematic review显示文摘 | LLOYD LJ LANGLEY-EVANS SC MCMULLEN S | 2012 | Inter J Obes2012，36，1： | 1 |
| 10 | Induction and function of the phage shock protein extracytoplasmic stress re- sponse in Escherichia coli显示文摘 | Jovahovic G Lloyd LJ Stumpf MPH | 2006 | J Biol Chem2006，281，21： | 1 |
| 11 | Childhood obe- sity and risk of the adult metabolic syndrome: A systematic review 显示文摘 | LLOYD LJ LANGLEY-EVANS SC MCMULLEN S | 2012 | Int J Obes (Lond)2012，36，1： | 1 |
| 12 | T-lymphocyte invasiveness:Control byvoltage-gated Na+ channel activity显示文摘 | Fraser SP Diss JK Lloyd LJ | 2004 | FEBS Lett2004，569，13： | 1 |
| 13 | The myeobacterial cell wall-peptidoglyean and arabinogalactan显示文摘 | Alderwiek LJ Harrison J Lloyd GS | 2015 | Cold Spring Harb Per- speet Med2015，5，02： | 1 |
| 14 | Suppression of acute proinflammatory cytokine and chemokine upregulation by post-injury administration of a novel small molecule improves long-term neurologic outcome in a mouse model of traumatic brain injury显示文摘 | Lloyd E Somera-Molina K Van Eldik LJ | 2008 | J Neuroinflammation2008，5，： | 1 |
| 15 | The indentification of delays from referral to treatment as part of the cancer collaborative initiatives(Abstract)显示文摘 | Lloyd TDR Sutton CD Marshall LJ | 2002 | Colorectal Dis2002，4，1： | 1 |
| 16 | Application of cancer collaborative initiatives (Abstract)显示文摘 | Lloyd TDR Sutton CD Marshall LJ | 2002 | Colorectal Dis2002，4，1： | 1 |
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