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| 1 | Two soybean bHLH factors regulate response to iron deficiency显示文摘Iron is an indispensable micronutrient for plant growth and development. Limited bioavailability of Fe in the soil leads to iron deficiency chlorosis in plants and yield loss. In this study, two soybean basic helix-loophelix transcription factors, Gmb HLH57 and Gmb HLH300,were identified in response to Fe-deficiency. Both transcription factors are expressed in roots and nodules,and are induced by Fe deficiency; these patterns were confirmed in transgenic hairy roots expressing constructs of the endogenous promoters fused to a GUS reporter gene. Bimolecular fluorescence complementation, yeast two-hybrid and coimmunoprecipitation(co-IP) assays indicated a physical interaction between Gmb HLH57 and Gmb HLH300. Studies on transgenic soybeans Reseoverexpressing Gmb HLH57 and Gmb HLH300 revealed that overexpression of each transcription factor, alone,results in no change of the responses to Fe deficiency,whereas overexpression of both transcription factors upregulated the downstream Fe uptake genes and increased the Fe content in these transgenic plants.Compared to wild type, these double overexpression transgenic plants were more tolerant to Fe deficiency.Taken together, our findings establish that Gmb HLH57 and Gmb HLH300 are important transcription factors involved in Fe homeostasis in soybean. | Lin Li Wenwen Gao Qi Peng Bin Zhou Qihui Kong Yinghui Ying Huixia Shou | 2018 | Journal of Integrative Plant Biology2018，60，7： | 10 |
| 2 | Characteristic of Torsional Vibration of Mill Main Drive Excited by Electromechanical Coupling显示文摘In the study of electromechanical coupling vibration of mill main drive system, the influence of electrical system on the mechanical transmission is considered generally, however the research for the mechanism of electromechanical interaction is lacked. In order to research the electromechanical coupling resonance of main drive system on the F3 mill in a plant, the cycloconverter and synchronous motor are modeled and simulated by the MTLAB/SIMULINK firstly, simulation result show that the current harmonic of the cycloconverter can lead to the pulsating torque of motor output. Then the natural characteristics of the mechanical drive system are calculated by ANSYS, the result show that the modal frequency contains the component which is close to the coupling vibration frequency of 42 Hz. According to the simulation result of the mechanical and electrical system, the closed loop feedback model including the two systems are built, and the mechanism analysis of electromechanical coupling presents that there is the interaction between the current harmonic of electrical system and the speed of the mechanical drive system. At last, by building and computing the equivalent nonlinear dynamics model of the mechanical drive system, the dynamic characteristics of system changing with the stiffness, damping coefficient and the electromagnetic torque are obtained. Such electromechanical interaction process is suggested to consider in research of mill vibration, which can induce strong coupling vibration behavior in the rolling mill drive system. | ZHANG Yifang YAN Xiaoqiang LIN Qihui | 2016 | Chinese Journal of Mechanical Engineering2016，29，1： | 8 |
| 3 | 会计师事务所合并与审计效率显示文摘得益于政策的鼓励,中国审计市场的集中度得以在短时间内大幅提升。以收入计算,中国本土最大的会计师事务所的市场份额从2006年的13.95%提升到2012年的36.12%,中国审计市场开始了由高度分散的审计市场向更加集中的审计市场的转变。已有关于会计师事务所合并的研究非常有限,其原因在于已有审计方面的研究均用审计费用来衡量审计投入。实际上,审计费用衡量的是审计服务的价格,而不是审计服务的投入成本。 | Qihui Gong Oliver Zhen Li Yupeng Lin Liansheng Wu | 2018 | 中国注册会计师2018，，7： | 2 |
| 4 | An experimental study on the choked flow characteristics of CO_(2) pipelines in various phases显示文摘High pressure pipeline transportation has been an established technology for economically transporting large amounts of CO_(2).However,there are still issues and associated risks that have to be effectively addressed and adequately understood.It is well known that a strong JouleThomson Cooling effect can occur when pressurized CO_(2) flows through a choke valve.Thus,to investigate the choking characteristics especially the temperature drop of high pressure CO_(2),a new laboratory scale experimental setup(total length of 14.85 m and the inner diameter of 15 mm)was constructed.Steady choked flow and transient choked flow tests were carried out respectively for pressurized CO_(2) in various initial phases.The phase transitions and temperature drop characteristics were then studied following the choked flow and the results show that the phase transitions in steady choked flow differs significantly from that in transient choked flow.For transient choked flow of various initial phases,all the flows downstream would transfer from single phase to gas-liquid twophase flow.Furthermore,the effect of water on transient choked flow of supercritical CO_(2) pipeline was investigated,and the phenomena of solid particles deposition was captured which was paramount importance of ensuring the safety operation of CO_(2)pipelines when throttling by the choke valves. | Yuxing Li Shuaiwei Gu Datong Zhang Qihui Hu Lin Teng Cailin Wang | 2021 | Chinese Journal of Chemical Engineering2021，34，4： | 1 |
| 5 | Sensitively detecting antigen of SARS-CoV-2 by NIR-Ⅱ fluorescent nanoparticles显示文摘Early detection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection is an efficient way to prevent the spread of coronavirus disease 2019(COVID-19).Detecting SARS-CoV-2 antigen can be rapid and convenient,but it is still challenging to develop highly sensitive methods for effective diagnosis.Herein,a lateral flow assay(LFA)based on fluorescent nanoparticles emitting in the second near-infrared(NIR-II)window is developed for sensitive detection of SARS-CoV-2 antigen.Benefiting from the NIR-II fluorescence with high penetration and low autofluorescence,such NIR-II based LFA allows enhanced signal-to-background ratio,and the limit of detection is down to 0.01 ng·mL^(−1)of SARS-CoV-2 antigen.In the clinical swab sample tests,the NIR-II LFA outperforms the colloidal gold LFA with higher overall percent agreement with the polymerase chain reaction test.The clinical samples with low antigen concentrations(~0.015–~0.068 ng·mL^(−1))can be successfully detected by the NIR-II LFA,but fail for the colloidal gold LFA.The NIR-II LFA can provide a promising platform for highly sensitive,rapid,and cost-effective method for early diagnosis and mass screening of SARS-CoV-2 infection. | Ruibin Hu Tao Liao Yan Ren Wenming Liu Rui Ma Xinyuan Wang Qihui Lin Guoxin Wang Yongye Liang | 2022 | Nano Research2022，15，8： | 0 |
| 6 | A pan-coronavirus peptide inhibitor prevents SARS-CoV-2 infection in mice by intranasal delivery显示文摘Coronaviruses(Co Vs)have brought serious threats to humans,particularly severe acute respiratory syndrome coronavirus 2(SARS-Co V-2),which continually evolves into multiple variants.These variants,especially Omicron,reportedly escape therapeutic antibodies and vaccines,indicating an urgent need for new antivirals with pan-SARS-Co V-2 inhibitory activity.We previously reported that a peptide fusion inhibitor,P3,targeting heptad repeated-1(HR1)of SARS-Co V-2 spike(S)protein,could inhibit viral infections.Here,we further designed multiple derivatives of the P3 based on structural analysis and found that one derivative,the P315V3,showed the most efficient antiviral activity against SARS-Co V-2 variants and several other sarbecoviruses,as well as other human-Co Vs(HCo Vs).P315V3 also exhibited effective prophylactic efficacy against the SARS-Co V-2 Delta and Omicron variants in mice via intranasal administration.These results suggest that P315V3,which is in PhaseⅡclinical trial,is promising for further development as a nasal pan-SARS-Co V-2 or pan-Co Vs inhibitor to prevent or treat CoV diseases. | Lili Wu Anqi Zheng Yangming Tang Yan Chai Jiantao Chen Lin Cheng Yu Hu Jing Qu Wenwen Lei William Jun Liu Guizhen Wu Shaogui Zeng Hang Yang Qihui Wang George Fu Gao | 2023 | Science China(Life Sciences)2023，66，10： | 0 |
| 7 | Mako:A Graph-based Pattern Growth Approach to Detect Complex Structural Variants显示文摘Complex structural variants(CSVs) are genomic alterations that have more than two breakpoints and are considered as the simultaneous occurrence of simple structural variants.However,detecting the compounded mutational signals of CSVs is challenging through a commonly used model-match strategy.As a result,there has been limited progress for CSV discovery compared with simple structural variants.Here,we systematically analyzed the multi-breakpoint connection feature of CSVs,and proposed Mako,utilizing a bottom-up guided model-free strategy,to detect CSVs from paired-end short-read sequencing.Specifically,we implemented a graph-based pattern growth approach,where the graph depicts potential breakpoint connections,and pattern growth enables CSV detection without pre-defined models.Comprehensive evaluations on both simulated and real datasets revealed that Mako outperformed other algorithms.Notably,validation rates of CSVs on real data based on experimental and computational validations as well as manual inspections are around 70%,where the medians of experimental and computational breakpoint shift are 13 bp and 26 bp,respectively.Moreover,the Mako CSV subgraph effectively characterized the breakpoint connections of a CSV event and uncovered a total of 15 CSV types,including two novel types of adjacent segment swap and tandem dispersed duplication.Further analysis of these CSVs also revealed the impact of sequence homology on the formation of CSVs.Mako is publicly available at http://gffzz188fe103f8f1460aspckukop5fx5o6bob.ffgz.tsg.suse.edu.cn/xjtu-omics/Mako. | Jiadong Lin Xiaofei Yang Walter Kosters Tun Xu Yanyan Jia Songbo Wang Qihui Zhu Mallory Ryan Li Guo Chengsheng Zhang The Human Genome Structural Variation Consortium Charles Lee Scott E.Devine Evan E.Eichler Kai Ye | 2022 | Genomics, Proteomics & Bioinformatics2022，20，1： | 0 |
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