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| 1 | Auxin response factor 6A regulates photosynthesis,sugar accumulation,and fruit development in tomato显示文摘Auxin response factors(ARFs)are involved in auxin-mediated transcriptional regulation in plants.In this study,we performed functional characterization of SlARF6A in tomato.SlARF6A is located in the nucleus and exhibits transcriptional activator activity.Overexpression of SlARF6A increased chlorophyll contents in the fruits and leaves of tomato plants,whereas downregulation of SlARF6A decreased chlorophyll contents compared with those of wild-type(WT)plants.Analysis of chloroplasts using transmission electron microscopy indicated increased sizes of chloroplasts in SlARF6A-overexpressing plants and decreased numbers of chloroplasts in SlARF6A-downregulated plants.Overexpression of SlARF6A increased the photosynthesis rate and accumulation of starch and soluble sugars,whereas knockdown of SlARF6A resulted in opposite phenotypes in tomato leaves and fruits.RNA-sequence analysis showed that regulation of SlARF6A expression altered the expression of genes involved in chlorophyll metabolism,photosynthesis and sugar metabolism.SlARF6A directly bound to the promoters of SlGLK1,CAB,and RbcS genes and positively regulated the expression of these genes.Overexpression of SlARF6A also inhibited fruit ripening and ethylene production,whereas downregulation of SlARF6A increased fruit ripening and ethylene production.SlARF6A directly bound to the SAMS1 promoter and negatively regulated SAMS1 expression.Taken together,these results expand our understanding of ARFs with regard to photosynthesis,sugar accumulation and fruit development and provide a potential target for genetic engineering to improve fruit nutrition in horticulture crops. | Yujin Yuan Xin Xu Zehao Gong Yuwei Tang Mengbo Wu Fang Yan Xiaolan Zhang Qian Zhang Fengqing Yang Xiaowei Hu Qichen Yang Yingqing Luo Lihua Mei Wenfa Zhang Cai-Zhong Jiang Wangjin Lu Zhengguo Li Wei Deng | 2019 | Horticulture Research2019，6，1： | 3 |
| 2 | The design and fault ride through control of un-interrupted DC-DC Autotransformer显示文摘This paper proposed a novel type of DC-DC converter, termed as un-interrupted DC Autotransformer(un-interrupted DC AUTO), which has the capability to ride through DC fault on either side of the converter without blocking any devices. Besides that, the adequately designed converter retains the advantages of DC AUTO technology, namely reduced investment cost and transmission loss. To begin with, the topology and basic attributes of the un-interrupted DC AUTO are illustrated. Then the design goals and the corresponding design procedures are analyzed. Furthermore, an effective control system is proposed to enable the converter steady operation during both normal and faulted conditions. Finally, a 1000 MW ±320 kV/±640 kV test system is built on PSCAD/EMTDC platform to verify the technical feasibility of the proposed converter and the effectiveness of the control strategy. | LI MengBo XIANG Wang ZUO WenPing YAO LiangZhong LIN WeiXing WEN JinYu | 2018 | Science China(Technological Sciences)2018，61，12： | 2 |
| 3 | Direct laser writing breaking diffraction barrier based on two-focus parallel peripheralphotoinhibition lithography显示文摘Direct laser writing(DLW)enables arbitrary three-dimensional nanofabrication.However,the diffraction limit poses a major obstacle for realizing nanometer-scale features.Furthermore,it is challenging to improve the fabrication efficiency using the currently prevalent single-focal-spot systems,which cannot perform high-throughput lithography.To overcome these challenges,a parallel peripheral-photoinhibition lithography system with a sub-40-nm two-dimensional feature size and a sub-20-nm suspended line width was developed in our study,based on two-photon polymerization DLW.The lithography efficiency of the developed system is twice that of conventional systems for both uniform and complex structures.The proposed system facilitates the realization of portable DLW with a higher resolution and throughput. | Dazhao Zhu Liang Xu Chenliang Ding Zhenyao Yang Yiwei Qiu Chun Cao Hongyang He Jiawei Chen Mengbo Tang Lanxin Zhan Xiaoyi Zhang Qiuyuan Sun Chengpeng Ma Zhen Wei Wenjie Liu Xiang Fu Cuifang Kuang Haifeng Li Xu Liu | 2022 | Advanced Photonics2022，4，6： | 1 |
| 4 | Depth recovery for unstructured farmland road image using an improved SIFT algorithm显示文摘Road visual navigation relies on accurate road models.This study was aimed at proposing an improved scale-invariant feature transform(SIFT)algorithm for recovering depth information from farmland road images,which would provide a reliable path for visual navigation.The mean image of pixel value in five channels(R,G,B,S and V)were treated as the inspected image and the feature points of the inspected image were extracted by the Canny algorithm,for achieving precise location of the feature points and ensuring the uniformity and density of the feature points.The mean value of the pixels in 5×5 neighborhood around the feature point at an interval of 45ºin eight directions was then treated as the feature vector,and the differences of the feature vectors were calculated for preliminary matching of the left and right image feature points.In order to achieve the depth information of farmland road images,the energy method of feature points was used for eliminating the mismatched points.Experiments with a binocular stereo vision system were conducted and the results showed that the matching accuracy and time consuming for depth recovery when using the improved SIFT algorithm were 96.48%and 5.6 s,respectively,with the accuracy for depth recovery of-7.17%-2.97%in a certain sight distance.The mean uniformity,time consuming and matching accuracy for all the 60 images under various climates and road conditions were 50%-70%,5.0-6.5 s,and higher than 88%,respectively,indicating that performance for achieving the feature points(e.g.,uniformity,matching accuracy,and algorithm real-time)of the improved SIFT algorithm were superior to that of conventional SIFT algorithm.This study provides an important reference for navigation technology of agricultural equipment based on machine vision. | Lijian Yao Dong Hu Zidong Yang Haibin Li Mengbo Qian | 2019 | International Journal of Agricultural and Biological Engineering2019，12，4： | 1 |
| 5 | One Novel Long Noncoding RNA lnc10 in Drosophila显示文摘With the development of computational methods and RNA sequencing technology for assembling the transcriptome,it is becoming clear that the mammal genome is pervasively transcribed,and large numbers of long noncoding RNAs(lncRNAs)composing the major part of the transcriptome have been identified(Ravasi et al.,2006;Birney et al.,2007;Kapranov et al.,2007;Guttman et al.,2009).As | Meixia Li Mengbo Xu Shengyun Wen Baoyan Bai Runsheng Chen Li Liu | 2014 | Journal of Genetics and Genomics2014，41，2： | 0 |
| 6 | SlMYB72 affects pollen development by regulating autophagy in tomato显示文摘The formation and development of pollen are among the most critical processes for reproduction and genetic diversity in the life cycle of f lowering plants.The present study found that SlMYB72 was highly expressed in the pollen and tapetum of tomato f lowers.Downregulation of SlMYB72 led to a decrease in the amounts of seeds due to abnormal pollen development compared with wild-type plants.Downregulation of SlMYB72 delayed tapetum degradation and inhibited autophagy in tomato anther.Overexpression of SlMYB72 led to abnormal pollen development and delayed tapetum degradation.Expression levels of some autophagy-related genes(ATGs)were decreased in SlMYB72 downregulated plants and increased in overexpression plants.SlMYB72 was directly bound to ACCAAC/ACCAAA motif of the SlATG7 promoter and activated its expression.Downregulation of SlATG7 inhibited the autophagy process and tapetum degradation,resulting in abnormal pollen development in tomatoes.These results indicated SlMYB72 affects the tapetum degradation and pollen development by transcriptional activation of SlATG7 and autophagy in tomato anther.The study expands the understanding of the regulation of autophagy by SlMYB72,uncovers the critical role that autophagy plays in pollen development,and provides potential candidate genes for the production of male-sterility in plants. | Mengbo Wu Qiongdan Zhang Guanle Wu Lu Zhang Xin Xu Xiaowei Hu Zehao Gong Yulin Chen Zhengguo Li Honghai Li Wei Deng | 2023 | Horticulture Research2023，10，3： | 0 |
| 7 | SIZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit显示文摘Chlorophylls and carotenoids are essential and bene fi cial substances for both plant and human health.Identifying the regulatory network of these pigments is necessary for improving fruit quality.In a previous study,we identi fi ed an R2R3-MYB transcription factor,SlMYB72,that plays an important role in chlorophyll and carotenoid metabolism in tomato fruit.Here,we demonstrated that the SlMYB72-interacting protein SlZHD17,which belongs to the zinc-fi nger homeodomain transcription factor family,also functions in chlorophyll and carotenoid metabolism.Silencing SIZHD 17 in tomato improved multiple bene fi cial agronomic traits,including dwar fi sm,accelerated fl owering,and earlier fruit harvest.More importantly,downregulating SIZHD17 in fruits resulted in larger chloroplasts and a higher chlorophyll content.Dual-luciferase,yeast one-hybrid and electrophoretic mobility shift assays clari fi ed that SlZHD17 regulates the chlorophyll biosynthesis gene SIPOR-B and chloroplast developmental regulator SITKN2 in a direct manner.Chlorophyll degradation and plastid transformation were also retarded after suppression of SIZHD17 in fruits,which was caused by the inhibition of SISGR1,a crucial factor in chlorophyll degradation.On the other hand,the expression of the carotenoid biosynthesis genes SIPSY1 and SIZISO was also suppressed and directly regulated by SlZHD17,which induced uneven pigmentation and decreased the lycopene content in fruits with SIZHD17 suppression at the ripe stage.Furthermore,the protein-protein interactions between SlZHD17 and other pigment regulators,including SlARF4,SlBEL11,and SlTAGL1,were also presented.This study provides new insight into the complex pigment regulatory network and provides new options for breeding strategies aiming to improve fruit quality. | Yuan Shi Xiaoqin Pang Wenjing Liu Rui Wang Deding Su Yushuo Gao Mengbo Wu Wei Deng Yudong Liu Zhengguo Li | 2021 | Horticulture Research2021，8，1： | 0 |
| 8 | Analytical and numerical analysis on the torsional performance of elliptically deformed tunnels in the longitudinal direction显示文摘Segmental lining structures constructed by shields/tunnel boring machines are often subjected to uneven longitudinal cross-section torsion as a result of eccentric external loads,which is extremely adverse to tunnel safety but has not received sufficient attention for a long time.To figure out the torsional performance of segmental tunnels,it is essential to assess the longitudinal torsional stiffness and active-torsion-rejection capability of a segmented tunnel.The aim of the paper is to derive an analytical solution to the longitudinal torsional stiffness of a segmental tunnel with existing elliptical deformation.The longitudinal torsional stiffness under different internal force combinations is deduced considering the longitudinal axial force and bending moment based on the equivalent continuous model and force balance equation.The validity of the analytical solution is verified by comparing it with finite element method results.Then,a parametric analysis,using the new analytical solution,is included to investigate the effect of the key parameters on torsional behaviors,including the segment size,the bolt size and the transverse bending stiffness,etc.It is found that:(1)the longitudinal torsional stiffness efficiency(LTSE)of the segmental tunnel decreases with the rise of segment thickness to diameter ratio but increases with the ring width to diameter ratio;(2)the LTSE reduces with the increase of the effective shear length but rises with the diameter of bolts;(3)the LTSE increases rapidly with the ratio of compression-torsion or bending-torsion.Furthermore,the envelope curve of the critical load(N0,M0)for a tunnel to actively resist a certain internal torque is given.The proposed solution can be easily utilized to determine the longitudinal torsional stiffness of segmental tunnels and is an effective tool for tunnel design and maintenance. | Yingbin Liu Shaoming Liao Mengbo Liu Lisheng Chen Zhiyi Li | 2023 | Underground Space2023，，5： | 0 |
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