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| 1 | From Golden Rice to aSTARice:Bioengineering Astaxanthin Biosynthesis in Rice Endosperm显示文摘Carotenoids are important phytonutrients with antioxidant properties,and are widely used in foods and feedstuffs as Supplements.Astaxanthin,a red-colored ketocarotenoid,has strong antioxidant activity and thus can benefit human health.However,astaxanthin is not produced in most higher plants.Here we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes,sZmPSY1,sPaCrtl,sCrBKT,and sHpBHY,which encode the enzymes phytoene synthase,phytoene desaturase,β-carotene ketolase,and β-carotene hydroxylase,respectively.Transgneic overexpression of two (sZmPSY1 and sPaCrtl),three (sZmPSY1,sPaCrtl and sCrBKT),and all these four genes driven by rice endosperm-specific promoters established the Carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm,from the yellow-grained β-caro- tene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice,respectively. Grains Of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant activity.These results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in therice endosperm.This work provides a Successful example for synthetic biology in plants and biofortification in crops;the biofortified rice products generated by this study could be consumed as health-promoting foods and processed tO produce dietary supplements. | Qinlong Zhu Dongchang Zeng Suize Yu Chaojun Cui Jiamin Li Heying Li Junyu Chen Runzhao Zhang Xiucai Zhao Letian Chen Yao-Guang Liu | 2018 | Molecular Plant2018，11，12： | 35 |
| 2 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 3 | BASIC THEORY AND METHOD OF WELDING ARC SPECTRAL INFORMATION显示文摘Arc spectral information is a rising information source which can solve many problems that can not be done with arc electric information and other arc information.It is of important significance to develop automatic control technique of welding process.The basic theory and methods on it play an important role in expounding and applying arc spectral information.Using concerned equation in plasma physics and spectrum theory,a system of equations including 12 equations which serve as basic theory of arc spectral information is set up.Through analyzing of the 12 equations,a basic view that arc spectral information is the reflection of arc state and state variation,and is the most abundant information resource reflecting welding arc process is drawn.Furthermore,based on the basic theory,the basic methods of test and control of arc spectral information and points out some applications of it are discussesed. | Li Junyue Li Zhiyong Li Huan Xue Haitao School of Material Science and Engineering,Tianjin University,Tianjin 300072, China | 2004 | Chinese Journal of Mechanical Engineering2004，17，2： | 9 |
| 4 | Air-sea heat flux exchange over the South China Sea under different weather conditions before and after southwest monsoon onset in 2000显示文摘With the data observed from the Second SCS Air-Sea Flux Experiment on the Xisha air-sea flux research tower, the radiation budget, latent, sensible heat fluxes and net oceanic heat budgetswere caculated before and after summer monsoon onset. It is discovered that, after summer monsoononset, there are considerable changes in air-sea fluxes, especially in latent heat fluxes and net oceanicheat budget. Furthermre, the analyzed results of five synoptic stages are compared. And the character-istics of the flux transfer during different stages around onset of South China Sea monsoon are discussed.The flux change shows that there is an oceanic heat accumulating process during the pre-onset and thebreak period, as same as oceanic heat losing process during the onset period. Moreover, latent fluxes,the water vapor moving to the continent, even the rainfall appearance in Chinese Mainland also can beinfluenced by southwester. Comparing Xisha fluxes with those obtained from the Indian Ocean and thewestern Pacific Ocean, their differences may be obeerved. It is the reason why SSTs can keep stableover the South China Sea while they decrease quickly over the Arabian Sea and the Bay of Bengal aftermonsoon onset. | Junyue Yan, Huadong Yao, Jianglong Li, Zhiyi Tang, Guorong Jiang, Wenyu Sha, Xunqiang Li, Yiguo Xiao National Climate Center, Beijing 100081, China. E-mail: yanjy@cma.gov.cnPLA University of Science Technology, Nanjing 211101, China | 2003 | Acta Oceanologica Sinica2003，22，3： | 8 |
| 5 | Catalytic ozonation treatment of papermaking wastewater by Ag-doped NiFe2O4 : Performance and mechanism显示文摘The catalytic ozonation treatment of secondary biochemical effluent for papermaking wastewater by Ag-doped nickel ferrite was investigated.Ag-doped catalysts prepared by sol-gel method were characterized,illustrating that Ag entirely entered the crystalline of Ni Fe2O4 and changed the surface properties.The addition of catalyst enhanced the removal efficiency of chemical oxygen demand and total organic carbon.The results of gas chromatography-mass spectrometer,ultraviolet light absorbance at 254 nm and threedimensional fluorescence excitation-emission matrix suggested that aromatic compounds were efficiently degraded and toxic substances,such as dibutyl phthalate.In addition,the radical scavenging experiments confirmed the hydroxyl radicals acted as the main reactive oxygen species and the surface properties of catalysts played an important role in the reaction.Overall,this work validated potential applications of Ag-doped Ni Fe2O4 catalyzed ozonation process of biologically recalcitrant wastewater. | Junyu Zhao Jiashun Cao Yujie Zhao Teng Zhang Di Zheng Chao Li | 2020 | Journal of Environmental Sciences2020，32，11： | 7 |
| 6 | Advanced liquid crystal devices for augmented reality and virtual reality displays:principles and applications显示文摘Liquid crystal displays(LCDs)and photonic devices play a pivotal role to augmented reality(AR)and virtual reality(VR).The recently emerging high-dynamic-range(HDR)mini-LED backlit LCDs significantly boost the image quality and brightness and reduce the power consumption for VR displays.Such a light engine is particularly attractive for compensating the optical loss of pancake structure to achieve compact and lightweight VR headsets.On the other hand,high-resolution-density,and high-brightness liquid-crystal-on-siilicon(LCoS)is a promising image source for the see-through AR displays,especially under high ambient lighting conditions.Meanwhile,the high-speed LCoS spatial light modulators open a new door for holographic displays and focal surface displays.Finally,the ultrathin planar diffractive LC optical elements,such as geometric phase LC grating and lens,have found useful applications in AR and VR for enhancing resolution,widening fiield-of-view,suppressing chromatic aberrations,creating multiplanes to overcome the vergence-accommodation conflict,and dynamic pupil steering to achieve gaze-matched Maxwellian displays,just to name a few.The operation principles,potential applications,and future challenges of these advanced LC devices will be discussed. | Kun Yin En-Lin Hsiang Junyu Zou Yannanqi Li Zhiyong Yang Qian Yang Po-Cheng Lai Chih-Lung Lin Shin-Tson Wu | 2022 | Light(Science & Applications)2022，11，7： | 6 |
| 7 | A WiFi Fingerprint Based High-Adaptability Indoor Localization via Machine Learning显示文摘For received signal strength(RSS)fingerprint based indoor localization approaches,the localization accuracy is significantly influenced by the RSS variance,device heterogeneity and environment complexity.In this work,we present a high-adaptability indoor localization(HAIL)approach,which leverages the advantages of both relative RSS values and absolute RSS values to achieve robustness and accuracy.Particularly,a backpropagation neural network(BPNN)is devised in HAIL to measure the fingerprints similarities based on absolute RSS values.With this aid,the characteristics of the applied area could be specially learned such that HAIL could be adaptive to different environments.The experiments demonstrate that HAIL achieves high localization accuracy with the average localization error of 0.87 m in the typical environments.Moreover,HAIL has the minimum amount of large errors and decreases the average localization error by about 30%~50%compared with the existing approaches. | Jianzhe Xue Junyu Liu Min Sheng Yan Shi Jiandong Li | 2020 | China Communications2020，17，7： | 5 |
| 8 | A novel selective autophagy receptor,CCDC50,delivers K63 polyubiquitination-activated RIG-I/MDA5 for degradation during viral infection显示文摘Autophagy is a con served process that delivers cytosolic substa nces to the lysosome for degradatio n,but its direct role in the regulation of antiviral inn ate immu nity remains poorly un derstood.Here,through high-throughput screeni ng,we discovered that CCDC50 functions as a previously unknown autophagy receptor that negatively regulates the type I interferon(IFN)signaling pathway initiated by RIG-I-like receptors(RLRs),the sensors for RNA viruses.The expression of CCDC50 is enhanced by viral infection,and CCDC50 specifically recognizes K63-polyubiquitinated RLRs,thus delivering the activated RIG-I/MDA5 for autophagic degradation.The associatio n of CCDC50 with phagophore membrane protei n LC3 is con firmed by crystal structure analysis.In contrast to other known autophagic cargo receptors that associate with either the LIR-docking site(LDS)or the UIMdocking site(UDS)of LC3,CCDC50 can bind to both LDS and UDS,representing a new type of cargo receptor.In mouse models with RNA virus infection,CCDC50 deficiency reduces the autophagic degradation of RIG-I/MDA5 and promotes type I IFN responses,resulting in enhaneed viral resistance and improved survival rates.These results reveal a new link between autophagy and antiviral innate immune resp on ses and provide additional in sights into the regulatory mecha nisms of RLR-mediated antiviral signaling. | Panpan Hou Kongxiang Yang Penghui Jia Lan Liu Yuxin Lin Zibo Li Jun Li Shuliang Chen Shuting Guo Ji'An Pan Junyu Wu Hong Peng Weijie Zeng Chunmei Li Yingfang Liu Deyin Guo | 2021 | Cell Research2021，31，1： | 5 |
| 9 | Characterization of VOC emissions from construction machinery and river ships in the Pearl River Delta of China显示文摘Speciated characterization of Volatile Organic Compounds (VOCs),including oxygenated VOCs (OVOCs),from construction machinery and river ships in China is currently lacking.In this regard,we conducted field measurement on speciated VOC (including OVOC) emissions from six construction machinery and five river ships in the Pearl River Delta (PRD) region to identify VOC emission characteristics.We noticed that OVOC emissions from construction machinery and ships accounted for more than 50%of the total VOC emissions,followed by alkenes,aromatics and alkanes.Formaldehyde and acetaldehyde were the most emission species,accounting for 61.8%-83.2%of OVOCs.For construction machinery,the fuel-based emission factors of roller,grader and pile driver were 3.12,3.12 and 7.36 g/kg,respectively.With the rigorous restraint by the national emission standards,VOC emissions of construction machinery had decreased considerably,especially during stageⅢ.Ozone formation potential was also significantly reduced due to the significant decrease in emissions of OVOCs and alkenes with higher reactivity.For river ships,the fuel-based emission factors of cargo ships and speedboat were 1.46 and 0.44 g/kg,respectively.VOC emissions from construction machinery and river ships in Guangdong Province in 2017 were 8851.0 and 4361.0 ton,respectively.This study filled the knowledge gaps of reactive gas emissions from different kinds of non-road mobile sources over the PRD,and more importantly,highlighted the necessity in adding OVOC measurement to give a complete and accurate depiction of reactive gas emissions from non-road mobile sources. | Richao Wang Zibing Yuan Junyu Zheng Cheng Li Zhijiong Huang Wenshi Li Yan Xie Yiran Wang Kaiyang Yu Lejun Duan | 2020 | Journal of Environmental Sciences2020，32，10： | 5 |
| 10 | A simple algorithm for testing the stability of periodic solutions of some nonlinear oscillators with large time delay显示文摘Periodic solutions occur commonly in linear or nonlinear dynamical systems. In some cases, the stability of a periodic solution holds if the rightmost characteristic root has negative real part. Based on the Lambert W function, this paper presents a simple algorithm for locating the rightmost characteristic root of periodic solutions of some nonlinear oscillators with large time delay. As application, the proposed algorithm is used to study the primary resonance and 1/3 subharmonic resonance of the Duffing oscillator under harmonic excitation and delayed feedback, as well as the control problem of the van der Pol oscillator under harmonic excitation by using delayed feedback, with a number of case studies. The main advantage of this algorithm is that though very simple in implementation, it works effectively with high accuracy even if the delay is large. | LI JunYu ZHANG Li WANG ZaiHua | 2011 | Science China(Technological Sciences)2011，54，8： | 5 |
| 11 | Metamorphic, deformation, fluids and geological significance of low-temperature retrograde mylonites of Diancangshan metamorphic massif along Ailaoshan-Red River strike-slip fault zone, Yunnan, China显示文摘Diancangshan metamorphic massif is one of the four metamorphic massifs developed along the Ailaoshan-Red River strike-slip fault zone, Yunnan, China. It has experienced multi-stage metamorphism and deformation, especially since the late Oligocene it widely suffered high-temperature ductile shear deformation and exhumation of the metamorphic rocks from the deep crust to the shallow surface. Based on the previous research and geological field work, this paper presents a detailed study on deformation and metamorphism, and exhumation of deep metamorphic rocks within the Diancangshan metamorphic massif,especially focusing on the low-temperature overprinted retrogression metamorphism and deformation of mylonitic rocks. With the combinated experimental techniques of optical microscope, electron backscatter diffraction attachmented on field-emission scanning electron microscopy and cathodoluminescence, our contribution reports the microstructure, lattice preferred orientations of the deformed minerals, and the changes of mineral composition phases of the superposition low-temperature retrograde mylonites. All these results indicate that:(1) Diancangshan deep metamorphic rock has experienced early high-temperature leftlateral shear deformation and late extension with rapid exhumation, the low-temperature retrogression metamorphism and deformation overprinted the high-temperature metamorphism, and the high-temperature microstructure and texture are in part or entirely altered by subsequent low-temperature shearing;(2) the superposition of low-temperature deformation-metamorphism occurs at the ductile-brittle transition; and(3) the fluid is quite active during the syn-tectonic shearing overprinted lowtemperature deformation and metamorphism. The dynamic recrystallization and/or fractures to micro-fractures result in the strongly fine-grained of the main minerals, and present strain localization in micro-domians, such as micro-shear zones in the mylonites. It is often accompanied by the decrease of rock strength and finally influences the rheology of the whole rock during further deformation and exhumation of the Diancangshan massif. | Xuemei CHENG Shuyun CAO Junyu LI Zunpu YU Yanlong DONG Meixia LV Junlai LIU | 2018 | Science China Earth Sciences2018，61，8： | 4 |
| 12 | Fermented mixed feed alters growth performance,carcass traits,meat quality and muscle fatty acid and amino acid profiles in finishing pigs显示文摘This study was conducted to investigate the effects of fermented mixed feed(FMF)on growth performance,carcass traits,meat quality,muscle amino acid and fatty acid composition and mRNA expression levels of genes related to lipid metabolism in finishing pigs.In the present study,144 finishing pigs(Duroc×Berkshire×Jiaxing Black)were randomly allocated to 3 dietary treatments with 4 replicate pens per group and 12 pigs per pen.The dietary treatments included a basal diet(CON),a basal diet+5%FMF and a basal diet+10%FMF.The experiment lasted 38 d after 4 d of acclimation.The results showed that 5%and 10%FMF significantly increased the average daily gain(ADG)of the females but not the males(P<0.05),but FMF supplementation showed no impact on carcass traits.Moreover,10%FMF supplementation increased the meat color45 min and meat color24 h values,while it decreased the shear force relative to CON(P<0.05).In addition,10%FMF significantly increased the contents of flavor amino acids(FAA),total essential AA(EAA),total non-EAA(NEAA)and total AA relative to CON(P<0.05).Furthermore,the diet supplemented with 10%FMF significantly increased the concentration of n-3 polyunsaturated fatty acids(PUFA),n-6 PUFA and total PUFA,and the PUFA to saturated fatty acids ratio(P<0.05),suggesting that FMF supplementation increased meat quality.Moreover,compared with the CON,10%FMF supplementation increased the mRNA expression of lipogenic genes,including CEBPα,PPARγ,SREBP1 and FABP4,and upregulated the expression of unsaturated fatty acid synthesis(ACAA1 and FADS2).Together,our results suggest that 10%FMF dietary supplementation improved the female pigs’growth performance,improved the meat quality and altered the profiles of muscle fatty acids and amino acids in finishing pigs.This study provides a reference for the production of high-quality pork. | Shiqi Liu Man Du Yuang Tu Wenjing You Wentao Chen Guoliang Liu Junyue Li Yizhen Wang Zeqing Lu Tenghao Wang Tizhong Shan | 2023 | Animal Nutrition2023，，1： | 4 |
| 13 | Rambutan-like hollow carbon spheres decorated with vacancy-rich nickel oxide for energy conversion and storage显示文摘Transition metal oxides hold great promise for lithium-ion batteries(LIBs)and electrocatalytic water splitting because of their high abundance and high energy density.However,designing and fabrication of efficient,stable,high power density electrode materials are challenging.Herein,we report rambutan-like hollow carbon spheres formed by carbon nanosheet decorated with nickel oxide(NiO)rich inmetal vacancies(denoted as h-NiO/C)as a bifunctional electrode material for LIBs and electrocatalytic oxygen evolution reaction(OER).When being used as the anode of LIBs,the h-NiO/C electrode shows a large initial capacity of 885mAh g−1,a robust stability with a high capacity of 817mAh g−1 after 400 cycles,and great rate capability with a high reversible capacity of 523mAh g−1 at 10Ag−1 after 600 cycles.Moreover,working as an OER electrocatalyst,the h-NiO/C electrode shows a small overpotential of 260mV at 10mAcm−2,a Tafel slope of 37.6mVdec−1 along with good stability.Our work offers a cost-effective method for the fabrication of efficient electrode for LIBs and OER. | Zixu Sun Xinghui Wang Hu Zhao See W.Koh Junyu Ge Yunxing Zhao Pingqi Gao Guangjin Wang Hong Li | 2020 | Carbon Energy2020，2，1： | 4 |
| 14 | 6G Service Coverage with Mega Satellite Constellations显示文摘The rapid development and continuous updating of the mega satellite constellation(MSC)have brought new visions for the future 6G coverage extension, where the global seamless signal coverage can realize ubiquitous services for user terminals. However, global traffic demands present nonuniform characteristics. Therefore, how to ensure the on-demand service coverage for the specific traffic demand, i.e., the ratio of traffic density to service requirement per unit area, is the core issue of 6G wireless coverage extension exploiting the MSC. To this regard, this paper first discusses the open challenges to reveal the future direction of 6G wireless coverage extension from the perspective of key factors affecting service coverage performance, i.e., the network access capacity, space segment capacity and their matchingrelationship. Furthermore, we elaborate on the key factors affecting effective matchings of the aforementioned aspects, thereby improving service coverage capability. | Min Sheng Di Zhou Weigang Bai Junyu Liu Jiandong Li | 2022 | China Communications2022，19，1： | 4 |
| 15 | Femtosecond mid-IR optical vortex laser based on optical parametric chirped pulse amplification显示文摘A femtosecond mid-infrared optical vortex laser can be used for high harmonic generation to extend cutoff energy to the kilo-electron-volt range with orbital angular momentum,as well as other secondary radiations.For these,we demonstrate a high-energy femtosecond 4μm optical vortex laser based on optical parametric chirped pulse amplification(OPCPA)for the first time.The optical vortex seed is generated from a femtosecond 4μm laser by a silicon spiral phase plate with the topological charge l of 1 before the stretcher.Through using a two-stage collinear OPCPA amplifier,the chirped vortex pulse is amplified to 12.4 m J with 200 nm full width at half-maximum bandwidth.After compression,the vortex laser pulse with 9.53 m J,119 fs can be obtained.Furthermore,the vortex characteristics of the laser beam are investigated and evaluated.This demonstration can scale to generate a higher-peak-power vortex mid-IR laser and pave a new way for high field physics. | JUNYU QIAN YUJIE PENG YANYAN LI PENGFEI WANG BEIJIE SHAO ZHE LIU YUXIN LENG RUXIN LI | 2020 | Photonics Research2020，8，3： | 4 |
| 16 | Novel application of bergapten and quercetin with anti-bacterial,osteogenesis-potentiating,and anti-inflammation tri-effects显示文摘The bacteria-mediated inflammatory conditions adversely affect the osseointegration process of endosseous implants,which can even lead to implant malfunction or failure.Local drug delivery has been designed to exert anti-inflammatory and antibacterial activities,but whether this strategy has an effect on the compromised osseointegration under inflammation has rarely been studied.The present study focused on the osteoinductive efficacy of two known phytoestrogens[bergapten(BP)and quercetin(QE)]on implant sites under multiple bacteria-infected conditions in situ.Furthermore,the gene expression profiles of rat bone mesenchymal stem cells(rBMSCs)treated with BP and QE in the presence of Porphyromonas gingivalis-derived lipopolysaccharide were identified.The results showed that both drugs,especially QE,had significant potentiating effects on promoting osteogenic differentiation of rBMSCs,resisting multiple pathogens,and reducing inflammatory activity.Meanwhile,RNA sequencing analysis highlighted the enriched gene ontology terms and the differentially expressed genes(Vps25,Il1r2,Csf3,Efemp1,and Ccl20)that might play essential roles in regulating the above tri-effects,which provided the basis for the drug delivery system to be used as a novel therapeutic strategy for integrating peri-implant health.Overall,our study confirmed that QE appeared to outperform BP in osteogenesis and bacterial killing but not in anti-inflammation.Moreover,both drugs possess favorable tri-effects and can serve as the pivotal agents for the drug delivery system to boost osseointegration at inflammatory implant sites. | Jianxu Wei Xiaomeng Zhang Yuan Li Xinxin Ding Yi Zhang Xue Jiang Hongchang Lai Junyu Shi | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 4 |
| 17 | m6A RNA methylation-mediated HNF3γreduction renders hepatocellular carcinoma dedifferentiation and sorafenib resistance显示文摘Hepatocyte nuclear factor 3γ(HNF3γ)is a hepatocyte nuclear factor,but its role and clinical significance in hepatocellular carcinoma(HCC)remain unclear.Herein,we report that HNF3γexpression is downregulated in patient HCC and inversely correlated with HCC malignancy and patient survival.Moreover,our data suggested that the HNF3γreduction in HCC could be mediated by METTL14-dependent m6A methylation of HNF3γmRNA.HNF3γexpression was increased during hepatic differentiation and decreased in dedifferentiated HCC cells.Interestingly,HNF3γdelivery promoted differentiation of not only HCC cells but also liver CSCs,which led to suppression of HCC growth.Mechanistic analysis suggested an HNF3γ-centered regulatory network that includes essential liver differentiation-associated transcription factors and functional molecules,which could synergistically facilitate HCC cell differentiation.More importantly,enforced HNF3γexpression sensitized HCC cells to sorafenib-induced growth inhibition and cell apoptosis through transactivation of OATP1B1 and OATP1B3 expression,which are major membrane transporters for sorafenib uptake.Clinical investigation showed that patient-derived HCC xenografts with high HNF3γexpression exhibited a sorafenib response and patients with high HCC HNF3γlevels benefited from sorafenib therapy.Together,these results suggest that HNF3γplays an essential role in HCC differentiation and may serve as a therapeutic target and predictor of sorafenib benefit in patients. | Tengfei Zhou Shichao Li Daimin Xiang Junyu Liu Wen Sun Xiuliang Cui Beifang Ning Xiao Li Zhuo Cheng Weiqi Jiang Cheng Zhang Xijun Liang Liang Li Xin Cheng Liu Hui Hongyang Wang Jin Ding | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 18 | Comparative transcriptional analysis provides insights of possible molecular mechanisms of wing polyphenism induced by postnatal crowding in Aphis gossypii显示文摘Background:Aphis gossypii is a worldwide sap-sucking pest with a variety of hosts and a vector of more than 50 plant viruses.The strategy of wing polyphenism,mostly resulting from population density increasing,contributes to the evolutionary success of this pest.However,the related molecular basis remains unclear.Here,we identified the effects of postnatal crowding on wing morph determination in cotton aphid,and examined the transcriptomic differences between wingless and wing morphs.Results:Effect of postnatal crowding on wing determination in A gossypii was evaluated firstly.Under the density of 5 nymphs·cm-2,no wing aphids appeared.Proportion of wing morphs rised with the increase of density in a certain extent,and peaked to 56.1% at the density of 20 nymphs·cm-2,and reduced afterwards.Then,transcriptomes of wingless and wing morphs were assembled and annotated separately to identify potentially exclusively or differentially expressed transcripts between these two morphs,in which 3 126 and 3 392 unigenes annotated in Nr(Non-redundant protein sequence) database were found in wingless or wing morphs exclusively.Moreover,3 187 up-and 1 880 down-regulated genes were identified in wing versus wingless aphid.Pathways analysis suggested the involvement of differentially expressed genes in multiple cellular signaling pathways involved in wing morphs determination,including lipid catabolic and metabolism,insulin,ecdysone and juvenile hormone biosynthesis.The expression levels of related genes were validated by the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR) soon afterwards.Conclusions:The present study identified the effects of postnatal crowding on wing morphs induction and demonstrated that the critical population density for wing morphs formation in A gossypii was 20 nymphs·cm-2.Comparative transcriptome analysis provides transcripts potentially expressed exclusively in wingless or wing morph,respectively.Differentially expressed genes between wingless and wing morphs were identified and several signaling pathways potentially involved in cotton aphid wing differentiation were obtained. | JI Jichao ZHANG Shuai LUO Junyu WANG Li ZHU Xiangzhen ZHANG Kaixin ZHANG Lijuan CUI Jinjie | 2019 | Journal of Cotton Research2019，2，3： | 3 |
| 19 | Ternary organic solar cells based on polymer donor,polymer acceptor and PCBM components显示文摘In this work,ternary organic solar cells(OSCs)combining a fullerene derivative PC71BM with a nonfullerene acceptor N2200-F blended with a polymer donor PM6 were reported.Compared with the binary systems,the highest power conversion efficiency(PCE)of 8.11%was achieved in ternary solar cells with 30 wt%N2200-F content,mainly due to the improved short-circuit current density(Jsc)and fill factor(FF).Further studies showed that the improved Jsc could attribute to the complementary abso rption of the two acceptors and the enhanced FF was originated from the higher hole mobility and the fine-tuned morphology in the ternary system.These results demonstrate that the combination of fullere ne and nonfullerene acceptors in ternary organic solar cells is a promising approach to achieve high-performance OSCs. | Feng Liu Cheng Li Junyu Li Chao Wang Chengyi Xiao Yonggang Wu Weiwei Li | 2020 | Chinese Chemical Letters2020，31，3： | 3 |
| 20 | Task Offloading Strategy with Emergency Handling and Blockchain Security in SDN-Empowered and Fog-Assisted Healthcare IoT显示文摘With the rapid advancement of the Internet of Things(IoT),the typical application of wireless body area networks(WBANs)based smart healthcare has drawn wide attention from all sectors of society.To alleviate the pressing challenges,such as resource limitations,low-latency service provision,mass data processing,rigid security demands,and the lack of a central entity,the advanced solutions of fog computing,software-defined networking(SDN)and blockchain are leveraged in this work.On the basis of these solutions,a task offloading strategy with a centralized low-latency,secure and reliable decision-making algorithm having powerful emergency handling capacity(LSRDM-EH)is designed to facilitate the resource-constrained edge devices for task offloading.Additionally,to well ensure the security of the entire network,a comprehensive blockchain-based two-layer and multidimensional security strategy is proposed.Furthermore,to tackle the inherent time-inefficiency problem of blockchain,we propose a blockchain sharding scheme to reduce system time latency.Extensive simulation has been conducted to validate the performance of the proposed measures,and numerical results verify the superiority of our methods with lower time-latency,higher reliability and security. | Junyu Ren Jinze Li Huaxing Liu Tuanfa Qin | 2022 | Tsinghua Science and Technology2022，27，4： | 3 |
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