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| 1 | Biosensor-based assay of exosome biomarker for early diagnosis of cancer显示文摘Cancer imposes a severe threat to people’s health and lives,thus pressing a huge medical and economic burden on individuals and communities.Therefore,early diagnosis of cancer is indispensable in the timely prevention and effective treatment for patients.Exosome has recently become an attractive cancer biomarker in noninvasive early diagnosis because of the unique physiology and pathology functions,which reflects remarkable information regarding the cancer microenvironment,and plays an important role in the occurrence and evolution of cancer.Meanwhile,biosensors have gained great attention for the detection of exosomes due to their superior properties,such as convenient operation,real-time readout,high sensitivity,and remarkable specificity,suggesting promising biomedical applications in the early diagnosis of cancer.In this review,the latest advances of biosensors regarding the assay of exosomes were summarized,and the superiorities of exosomes as markers for the early diagnosis of cancer were evaluated.Moreover,the recent challenges and further opportunities of developing effective biosensors for the early diagnosis of cancer were discussed. | Ying Deng Zhaowei Sun Lei Wang Minghui Wang Jie Yang Genxi Li | 2022 | Frontiers of Medicine2022，16，2： | 3 |
| 2 | Aptamer-based and DNAzyme-linked colorimetric detection of cancer cells显示文摘This paper reports a novel method to detect human leukemic lymphoblasts(CCRF-CEM cells).While the aptamer of the cancer cells was employed as the recognition element to target cancer cells,peroxidaseactive DNAzyme was used as the sensing element to produce catalysis-induced colorimetric signals.The elegant architecture integrating the aptamer and DNAzyme made it feasible to detect cancer cells easily and rapidly by the color change of the substrate for DNAzyme.Experimental results showed that 500 cells can well indicate the cancer,while as control,250,000 Islet Island Beta cells only show tiny signals,suggesting that the method proposed in this paper has considerable sensitivity and selectivity.Furthermore,since it does not require expensive apparatus,or modification or label of DNA chains,the method we present here is also costeffective and conveniently operated,implying potential applications in future cancer diagnosis. | Xiaoli Zhu Ya Cao Zhiqiang Liang Genxi Li | 2010 | Protein & Cell2010，1，9： | 2 |
| 3 | A green method of staining DNA in polyacrylamide gel electrophoresis based on fluorescent copper nanoclusters synthesized in situ显示文摘染色的 nucleic 酸的安全染长被认出了是一个问题。广泛的努力被作了寻找最流行却有毒的染色的染料的选择, ethidium 溴化物(EtBr ) 。然而,到目前为止不染色能被保证足够地安全的方法被开发了。在这份报纸,我们在 polyacrylamide 胶化电气泳动报导 DNA 的一个绿染色方法,在的地方在 situ 合成模板 DNA 荧光灯在胶化的铜 nanoclusters (CuNCs ) 被完成在紫外光下面使 DNA 乐队可见。而且,这个染色的方法的表演的全面研究被进行了,试验性的结果证明它有有利敏感,稳定性,和可用性。在我们的动物实验,同时,象综合 CuNCs 一样的二试剂(铜硫酸盐和维生素酸) 被证明了在与皮肤的接触无毒。另外,在这个工作采用的所有试剂是容易地可得到、低的费用,并且过程简单执行。因此,染色方法基于的这篇小说在里面 situ 合成模板 DNA 荧光灯 CuNCs 有许多潜在的应用程序。 | Xiaoli Zhu Hai Shi Yalan Shen Bin Zhang Jing Zhao Genxi Li | 2015 | Nano Research2015，8，8： | 2 |
| 4 | Fabrication of nanozyme@DNA hydrogel and its application in biomedical analysis显示文摘Nanozymes 由于容易的准备和低费用的优点收到了大注意。不同于乐意地适应生理的环境的自然的酶,人工的 nanozymes 是容易的在复杂临床的样品使钝化(例如,浆液) ,它可以损坏催化能力并且因而在生物医学的分析限制申请。为了征服这个问题,在这研究,我们由把 nanozymes 合并到纯 DNA hydrogel 制作了新奇 nanozyme@DNA hydrogel 建筑学。金 nanoparticles (AuNPs ) 作为模型 nanozyme 被采用。当他们被 hydrogel 保护,结果显示在 DNA hydrogel 合并的 AuNPs 在浆液保留他们的催化能力,而 AuNPs 完全独自在浆液失去催化能力。在浆液的氢过氧化物和葡萄糖的察觉基于 AuNPs@DNA hydrogel 的催化作用被完成。分别地,各个的察觉限制到达 1.7 和 38 M 它等于用自然的酶获得的价值。除机制以外,一些另外的优点例如再循环能力和可获得性,也被探索了。这 nanozyme@DNA hydrogel 体系结构可以在复杂生理的样品为生物医学的分析象 nanozymes 的申请一样为 nanozymes 的利用有一个大潜力。 | Xiaoli Zhu Xiaoxia Mao Zihan Wang Chang Feng Guifang Chen Genxi Li | 2017 | Nano Research2017，10，3： | 2 |
| 5 | Interaction between curcumin and mimetic biomembrane显示文摘Curcumin,a major bioactive compound in turmeric,has a broad spectrum of antioxidant,anticarcinogenic,antimutagenic and anti-inflammatory properties.At the molecular level,curcumin modulates many structurally unrelated membrane proteins through several signaling pathways.Curcumin has been suggested to change the properties of cell membranes and affect the membrane-bound proteins indirectly;however,the detailed mechanism has yet to be investigated.In this paper,self-assembled bilayer lipid membranes are artificially constructed on the surface of a gold electrode to mimic biomembranes,and interaction between the supported membranes and curcumin is studied electrochemically.Results show that curcumin interacts with the membranes strongly,in a concentration-dependent manner.At low concentrations,curcumin tends to insert into the outer monolayer only,while at high concentrations,it may also begin to penetrate the inner monolayer.The results obtained in this work may enhance our understanding of the effect of curcumin,and possibly flavonoids,on cell membranes and membrane proteins. | CHEN GuiFang CHEN YangYang YANG NaNa ZHU XueJun SUN LiZhou LI GenXi | 2012 | Science China(Life Sciences)2012，55，6： | 2 |
| 6 | Iodide modified silver flectrode and its application to the electroanalysis of hemoglobin显示文摘 | Li Genxi Zhuang Yi | 2000 | Electroanalysis2000，12，： | 1 |
| 7 | Isolation and determination of p-hydroxybenzoylcholine in traditional Chinese medicine Semen sinapis Albae显示文摘 | Lifang Liu Tao Liu Genxi Li Qiang Wang Tzibun Ng | 2003 | Analytical and Bioanalytical Chemistry2003，，6： | 1 |
| 8 | A colorimetric method for α-glucosidase activity assay and its inhibitor screening based on aggregation of gold nanoparticles induced by specific recognition between phenylenediboronic acid and 4-aminophenyl-α-D- glucopyranoside显示文摘一个比色的方法为屏蔽的 -glucosidase 活动试金和它的禁止者被建立了。方法基于在 1,4-phenylenediboronic 酸(PDBA ) 和 4-aminophenyl--d-glucopyranoside (pAPG ) 之间的特定的识别,它可以导致 pAPG-functionalized 金牌 nanoparticles (AuNPs ) 的聚集完成测试溶液的颜色变化。因为 pAPG 是 -glucosidase, 的底层,自从在 PDBA 和 4-aminobenzene 之间没有协作反应, AuNPs 的聚集将被 -glucosidase 影响, pAPG 的 hydrolyzed 产品由酶催化。因此,为 -glucosidase 活动的试金的一个简单、容易操作的比色的方法能被开发。在优化试验性的条件下面,在在 520 nm 的到那的 650 nm 的波长的吸收度的比率与 0.004 U/mL 的最低察觉限制从 0.05 ~ 1.1 U/mL 在一个范围以内与 -glucosidase 活动线性地变化。而且用建议方法,在 -glucosidase 活动的法国的酸和橡黄素的抑制效果能与 IC 被测试 < 潜水艇 class= “ a-plus-plus ” > 1.16 公里和 1.82 M 的 50 价值分别地。因此,方法不仅为 -glucosidase 活动的察觉,而且为屏蔽它的禁止者有一个大潜力。 | Juan Zhang Ying Liu Jun Lv Genxi Li | 2015 | Nano Research2015，8，3： | 1 |
| 9 | Determination and depletion of amoxicillin residues in eggs显示文摘 | Kaizhou Xie Min Zhao Huisheng Guo Xiaojie Zhang Yingying Sun Aihua Li Xing Xie Genxi Zhang Guojun Dai Jinyu Wang | 2013 | Food Additives & Contaminants: Part A2013，，4： | 1 |
| 10 | An electrochemical biosensor constructed by nanosized silver particles doped sol-gel film显示文摘 | Xu Jinzhong Zhang Yan Li Genxi | | 0，，6： | 1 |
| 11 | Electrochemical Method to Characterize Multidrug Resistance显示文摘 | LU Shan NING Limin GAO Tao WU Xiaolan YIN Yongmei LI Genxi | 2014 | Chemical Research in Chinese Universities2014，30，3： | 1 |
| 12 | Iodide Modified Silver Electrode mad its Application to the Electroanalysis of Hemoglobin 显示文摘 | ChunhaiFan Genxi Li Yi Zhuang | 2000 | Electnmnalysis2000，12，3： | 1 |
| 13 | Integration of chemoselective ligation with enzymespecific catalysis: Saccharic colorimetric analysis using aminooxy/hydrazine-functionalized gold nanoparticles显示文摘这里,我们用 aminooxy/hydrazine-functionalized 金牌 nanoparticles (AO/AuNPs 或 H/AuNPs ) 基于 chemoselective 结扎和酶特定的催化作用的联合开发了一个糖合物的比色的方法。在半乳糖(女郎)的察觉, galactohexodialdose ( GHDA ),半乳糖 oxidase ( GalOx )催化产品,有一个醛组,它允许它 chemoselectively 由 oxime/hydrazone 按化学在 AO/AuNPs 或 H/AuNPs 的外部层与 aminooxy 或肼组反应形成 oxime 或 hydrozone 。通过 cis-diols 上的 1,4-phenylenediboronic 酸( PDBA )的特定的识别,因而, GHDA ,在 cis 形式包含二个氢氧根,能不仅与 AO/AuNPs 或 H/AuNPs ,而且与 PDBA 绑形成 boronate diester ,从而触发 AuNPs 的聚集并且引起相应颜色变化。当 GalOx 催化特定的底层,女郎的数量与 GHDA 和 AuNPs 聚集的程度的生产相关,因此允许一简单并且容易 operatable 为要发展的女郎察觉的比色的方法。在优化试验性的条件下面,在在 536 nm 的到那的 617 nm 的波长的吸收度的比率在大量 500 nM 以内与女郎集中的对数的价值线性地变化到 5 公里。而且,这个比色的方法与 21 nM 的察觉限制显示出反干扰能力和高敏感。因此,为精确、特定的比色的分析的一个通用平台能与酶通过 chemoselective 结扎的集成被建立特定的催化作用。 | Juan Zhang Jun Lv Xiaonan Wang Defeng Li Zhaoxia Wang Genxi Li | 2015 | Nano Research2015，8，12： | 1 |
| 14 | Third-generation biosensors based on the direct electron transfer of proteins显示文摘 | ZHANG Wenjun LI Genxi | 2004 | Anal Sci2004，20，4： | 1 |
| 15 | Peptide-induced bio-mineralization as a bio-mimetic means of detecting proteins in a mineralizing bio-context显示文摘病理学的简历矿化作用能被象 preeclampsia 那样的疾病导致。由这些自然地发生的简历矿化作用进程启发了,我们设计了进程叫的控制蛋白质的肽汇编双人脚踏车肽模板简历矿化作用。这种技术在目标蛋白质的活动提供 bio-context-associated 数据,并且在联系生物微型环境便于蛋白质功能的评估。它能为 biosensing 应用程序与高敏感提供有效信号扩大是简历模仿的进程导致 Ag 装饰 nanoparticle 的肽 nanowires 的形成。因而,高温度的要求因素 A1 (HtrA1 ) 能是在临床的浆液样品的 assayed 份量上为 preeclampsia 的诊断和疾病的改进处理提供信息。结果建议这个过程在临床的实践为使用有可观的潜力。 | Yuanyuan Zhang Hao Li Yue Huang Lizhou Sun Genxi Li | 2016 | Nano Research2016，9，5： | 0 |
| 16 | Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage显示文摘Evaluating tumor development is of great importance for clinic treatment and therapy.It has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the cell.So,in this work,cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids,i.e.,N-glycolylneuraminic acid(Neu5Gc)and N-acetylneuraminic acid(Neu5Ac).Specifically,PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface,followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra agglutinin.Subsequently,the activated Cas12a indiscriminately cleaves DNA,resulting in the release of PbS and CdS quantum dots,both of which can be simultaneously detected by anodic stripping voltammetry.Consequently,Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL,respectively.Therefore,a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface,which can be further used as a tool to identify bladder cancer cells at different development stages.Our method to evaluate tumor development is simple and easy to be operated,so it can be potentially applied for the detection of tumor occurrence and development in the future. | Liangfen Cheng Fuhan Yang Longfei Tang Lelin Qian Xu Chen Feng Guan Juan Zhang Genxi Li | 2022 | Research2022，，3： | 0 |
| 17 | A novel strategy to inhibit the reproduction and translation of hepatitis C virus显示文摘Hepatitis C virus (HCV), a positive single-stranded RNA virus, is a major cause of liver disease in humans. Herein we report a novel strategy to inhibit the reproduction and translation of HCV using a short RNA, named an Additional RNA, to activate the endonuclease activity of Argonaute 2 (Ago2). In the presence of the Additional RNA, the HCV genome RNA has the requisite 12 nucleotides of base-pairing with microRNA-122. This activates the endonuclease activity of Ago2, resulting in cleavage and release of the HCV genome RNA from Ago2 and microRNA-122. The free HCV genome RNA would be susceptible to intracellular degradation, effectively inhibiting its reproduction and translation. This study presents a new method to inhibit HCV that may hold great potential for HCV treatment in the future. | DUAN AiPing NING LiMin LI Chao HOU YaFei YANG NaNa SUN LiZhou LI GenXi | 2013 | Science China(Life Sciences)2013，56，4： | 0 |
| 18 | Chinese protein scientists met in Hefei for the 4th Chinese Protein Society Symposium显示文摘Some 600 scholars and students in protein science from around 40 institutions across China gathered at the 4th Symposium of the Chinese Protein Society held during October 12 14,2013,in the city of Hefei,Anhui Province,China,with the theme'Protein and Human Health'.This symposium was organized by the Chinese Protein Society, | LI GenXi CHANG ZengYi | 2013 | Science China(Life Sciences)2013，56，12： | 0 |
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