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| 1 | Endoscopic ultrasound guided fine needle aspiration and useful ancillary methods显示文摘The role of endoscopic ultrasound(EUS) in evaluating pancreatic pathology has been well documented from the beginning of its clinical use. High spatial resolution and the close proximity to the evaluated organs within the mediastinum and abdominal cavity allow detection of small focal lesions and precise tissue acquisition from suspected lesions within the reach of this method. Fine needle aspiration(FNA) is considered of additional value to EUS and is performed to obtain tissue diagnosis. Tissue acquisition from suspected lesions for cytological or histological analysis allows, not only the differentiation between malignant and non-malignant lesions, but, in most cases, also the accurate distinction between the various types of malignant lesions. It is well documented that the best results are achieved only if an adequate sample is obtained for further analysis, if the material is processed in an appropriate way, and if adequate ancillary methods are performed. This is a multi-step process and could be quite a challenge in some cases. In this article, we discuss the technical aspects of tissue acquisition by EUS-guided-FNA(EUS-FNA), as well as the role of an on-site cytopathologist, various means of specimen processing, and the selection of the appropriate ancillary method for providing an accurate tissue diagnosis and maximizing the yield of this method. The main goal of this review is to alert endosonographers, not only to the different possibilities of tissue acquisition, namely EUS-FNA, but also to bring to their attention the importance of proper sample processing in the evaluation of various lesions in the gastrointestinal tract and other accessible organs. All aspects of tissue acquisition(needles, suction, use of stylet, complications, etc.) have been well discussed lately. Adequate tissue samples enable comprehensive diagnoses, which answer the main clinical questions, thus enabling targeted therapy. | Mario Tadic Tajana Stoos-Veic Rajko Kusec | 2014 | World Journal of Gastroenterology2014，20，39： | 6 |
| 2 | Comparison of karyotype analysis and RT-PCR for AML1/ETO in 204 unselected patients with AML显示文摘 | Mitterbauer M Kusec R Schwarzinger I | 1998 | Ann Hematol1998，76，34： | 1 |
| 3 | Loealization of estrogen receptor-α in human and rabbit skeletal tissues 显示文摘 | Kusec V Virdi AS Prince R | 1926 | JAMA1926，87，： | 1 |
| 4 | AML1/ETO fusion mRNA can be detected in remission blood samples of all patients with t(8;21) acute myeloid leukemia after chemotherapy or autologous bone marrow transplantation显示文摘 | Kusec R Laczika K Knobl P | 1994 | Leukemia1994，8，5： | 1 |
| 5 | More on prognostic significance of FLT3-ITD size in acute myeloid leukemia ( AML ) 显示文摘 | Kusec R Jaksic O Ostojic S | 2006 | Blood2006，108，1： | 1 |
| 6 | MicroRNA expression in multiple myeloma is associated with genetic subtype,isotype and survival显示文摘 | Chi J Ballabio E Chen XH Kusec R Taylor S Hay D | | 0，，： | 1 |
| 7 | More on prognostic significance of FLT3/ITD size in acute myeloid leukemia(AML) 显示文摘 | Kusec R Jaksic O Ostojic S | 2006 | Blood2006，108，1： | 1 |
| 8 | Bone morphogenetic proteinsand receptors are over-expressed in bone-marrow cells of multiplemyeloma patients and support myeloma cells by inducing ID genes显示文摘 | Grcevi觬D Kusec R Kovaci觬N | 2010 | Leu Res2010，34，6： | 1 |
| 9 | Humoral SPARC/osteonectin pro- tein in plasma cell dyscrasias显示文摘 | Turk N Kusec R Jaksic B | 2005 | Ann Hematol2005，84，5： | 1 |
| 10 | Changes ofAXIN-l and beta-catenin in neuroepithelial brain tumors显示文摘 | Nikuseva Martic T Pecina-Slaus N Kusec V | 2010 | Pathol Oncol Res2010，16，： | 1 |
| 11 | Serum levels ofVEGF and bFGF in hypoxic patients with exacerbated COPD显示文摘 | PAVLISA G PAVLISA G KUSEC V | 2010 | Eur Cytokine Netw2010，21，2： | 1 |
| 12 | Osteocalcin:biochemical consideration and clinical application显示文摘 | Kusec V | 2005 | Lijec Vjesn2005，127，56： | 1 |
| 13 | Expression of estrogen receptor-alphq in cells of the osteoclastic lineage 显示文摘 | Oreeffo RO Kusec V Virdi AS | 1999 | Histochem Cell Biol1999，111，2： | 1 |
| 14 | More on prognostic significance of FLT3/ITD size in acute myeloid leukemia (AML)显示文摘 | KUSEC R JAKSIC O OSTOJIC S | 2006 | Blood2006，108，1： | 1 |
| 15 | The localization of the functional glucocorticoid receptor alpha in human bone显示文摘 | Abu EO Homer A Kusec V | 2000 | J Clin Endocrinol Metab2000，85，2： | 1 |
| 16 | AML1/ETO fusion mRNA can be detected in remission blood samples of all patients with t (8;21) acute myeloid leukemia af- ter chemotherapy or autologous bone marrow transplantation 显示文摘 | Kusec R Laczika K Kntibl P | 1994 | Leukemia1994，8，5： | 1 |
| 17 | Bone morphogenetic proteins and receptors are over-expressed in bone-marrow cells of mutiple myeloma patients and support myeloma cells by inducing ID genes显示文摘 | Grcevic D Kusec R Kovacic N | 2010 | Leuk Res2010，34，6： | 1 |
| 18 | The localization of androgen receptors in human bone 显示文摘 | ABU EO HORNER A KUSEC V | 1997 | J Clin Endocrinol Metab1997，82，10： | 1 |
| 19 | Changes of AXIN-1 and beta-catenin in neuroepithelial brain tumors显示文摘 | Nikuseva Marti (c) T Pe (c)ina-Slaus N Kusec V | 2010 | Pathol Oncol Res2010，16，1： | 1 |
| 20 | Histopathological features of gastritis before and after treatment for Helicobacter pylori 显示文摘 | Jakie-Razumovic J Tentor D Kusec D | 2000 | Croat Med J2000，41，2： | 1 |
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