|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 褪黑素合成酶基因转化烟草及转化植株抗氧化系统变化显示文摘通过根癌农杆菌(含植物表达载体YXu55)介导的转化技术,将褪黑素生物合成酶-芳烷基胺N-乙酰转移酶(Arylalkylamine N-acetyltransferase,AANAT)与羟基吲哚O-甲基转移酶(Hydroxyindole O-methyltransferase,HIOMT)基因导入到烟草(秦烟95)中。对所获得的庆大霉素抗性烟草株系进行Southern blotting和RT-PCR分子生物学检测,结果表明,AANAT-HIOMT基因已成功地整合到烟草基因组中,并且可以在mRNA水平上进行转录。用反相高效液相色谱法(RP-HPLC)测定转化株系的褪黑素含量表明,转AANAT-HIOMT基因烟草株系的褪黑素含量均明显高于pZP122(不含AANAT和HIOMT基因的空白质粒)转基因株系和未转基因的对照植株,证明AANAT-HIOMT基因在转基因植株中的表达增强了褪黑素的合成能力。对不同株系抗氧化系统的部分指标进行了测定,并与其亲本对照植株比较,发现AANAT-HIOMT基因在转基因植物中的表达引起超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性增加,谷胱甘肽(GSH)浓度以及总抗氧化能力升高,且没有引起膜脂质过氧化物丙二醛(MDA)的变化,说明转基因烟草抗氧化潜能得到提高。 | 王英娟 贾敬芬 步怀宇 赵宇玮 许耀 Carl Hirschie Johnson Jan Kolár | 2009 | 生物工程学报2009,25,7: | 3 |
| 2 | 西地那非能改善慢性肾功能衰竭大鼠的勃起功能显示文摘勃起功能障碍(ED)和慢性肾衰竭(CRF)之间的关系已在几个研究中被报道。本研究旨在探讨长期使用西地那非是否能增强CRF诱导大鼠的勃起能力。此外,我们评估了该治疗对某些分子的影响,这些分子被认为在勃起生理学及CRF相关的ED中起关键作用。研究分三组:(1)年龄配对的对照组,(2)CRF诱导的大鼠模型组,(3)CRF诱导大鼠给予西地那非慢性给药组((5 mg /kg口服6周[CRF诱导后6周开始治疗])。 3个月时,所有的动物进行海绵体神经刺激(CNS)来评估勃起功能。在所有大鼠组进行,阴茎组织晚期糖化终产物(AGE的) / 5-羟甲基-2-糠醛,丙二醛(MDA),cGMP(ELISA),诱导型一氧化氮合酶(iNOS)和神经元型NOS(nNOS的)(Western印迹)分析。CRF诱导大鼠与对照组相比,勃起功能有显著降低(P 〈0.05)。CRF诱导大鼠西地那非治疗组(第3组),海绵体内压(ICP)和曲线下面积的
增加量,大于CRF诱导大鼠组(第2组)。此外,西地那非治疗下调了AGE,MDA和iNOS的水平,同时保持了CRF诱导阴茎组织的nNOS和cGMP含量。西地那非治疗组AGE,MDA,iNOS的下降和nNOS,cGMP水平的增加,增加了ICP和海绵体神经刺激(CNS)的总ICP,使得CRF诱导大鼠阴茎勃起功能得到改善。本研究的结果表明慢性西地那非给药对CRF诱导大鼠的勃起功能有治疗效果。 | Nilgun Gurbuz Arif Kol Tumay Ipekci Erhan Ates Asli Baykal Mustafa F Usta | 2015 | Asian Journal of Andrology2015,17,5: | 3 |
| 3 | 变摩擦-摩擦摆支座减震机理及减震效果有限元分析显示文摘将传统摩擦摆支座的球形滑面划分为n个圆环区域,研发一种新型变摩擦-摩擦摆支座,通过在不同环面上铺设不同材料从而实现摩擦系数的变化;通过理论及数值方法分析变摩擦-摩擦摆支座的减震机理,并探讨n变化时支座减震机理的变化规律;将n=6的新型变摩擦-摩擦摆支座应用到K8型单层球面网壳结构中,研究变摩擦-摩擦摆支座网壳结构的抗震性能,并分析传统及新型变摩擦-摩擦摆支座结构减震效果的差别。与传统摩擦摆支座相比,变摩擦-摩擦摆支座刚度和阻尼具有良好的自适应特性,并且随着n的增大,支座的自适应特性逐渐增强;变摩擦-摩擦摆支座单层球面网壳结构具有良好的抗震性能,与传统摩擦摆支座网壳结构相比,变摩擦-摩擦摆支座结构的减震效果更优。 | Kol Channara 张玉霞 孔德文 范峰 支旭东 | 2018 | 施工技术2018,47,14: | 3 |
| 4 | Local symmetries of finite type hypersurfaces in C^2 Dedicated to Professor Sheng GONG on the occasion of his 75th birthday显示文摘 | KOL■ Martin | 2006 | Science China Mathematics2006,49,11: | 2 |
| 5 | Clinical outcome of instrumented fusion for the treatment of failed back surgery syndrome: a case series of 100 patients显示文摘 | Mark Arts Nicola Kols Suzanne Onderwater Wilco Peul | 2012 | Acta Neurochirurgica2012,,7: | 2 |
| 6 | Hypoxia - induced mito- genic factor (HIMF/FIZZ1/RELM alpha) recruits bone marrow -derived cells to the murine pulmonary vasculature 显示文摘 | ANGELINI D J SU Q KOL I A | 2010 | PLoS One2010,5,11: | 1 |
| 7 | Long-term investigation of nonsurgical treatment for thoracolumbar and lumbar burst fractures:an outcome analysis in sight of spinopelvic balance显示文摘 | Kol er H Acosta F Hempfing A | | 0,,08: | 1 |
| 8 | Interleukin-17 family members and inflammation显示文摘 | Kol s JK Lindén A | | 0,,04: | 1 |
| 9 | Angiotensin 2 increases mitogen activator messenger RMA in cultured rat aortic smooth muscle cells 显示文摘 | Van Leeuwen R T J Kol A Andreotti F | 1994 | Circulation1994,90,: | 1 |
| 10 | Spatial variability of cadmium pH,organic matter in soil and its dependence on sampling scales显示文摘 | Kuzel S Nydl V Kolár L Tichy R | 1994 | Water Air and Soil Pollution1994,78,12: | 1 |
| 11 | Initial prescrotal approach for palpable cryptorchid testis:results during a 3-year period显示文摘 | Yucel S Celik O Kol A | | 0,,: | 1 |
| 12 | Effect of changes in insect hosts on the pathogenicity and spore formation in microsporidia显示文摘 | Kol chevskaya E N Issi I V | 1991 | Parazitologigya1991,25,6: | 1 |
| 13 | Addition of dexmedetomidine or lornoxieam to prilocaine in intravenous regionous anaesthesia for hand or forearm surgery: a randomize controlled study 显示文摘 | Kol IO Ozturk H Kaygusuz K | 2009 | Clin Durg Investig2009,29,2: | 1 |
| 14 | Cutting edge: Heat shock protein (HSP) 60 activates the innate immune response:CD14 is an essential receptor for HSP60 activation of mononuclear cells显示文摘 | Lichtman AH Finberg RW | 2000 | J Immunol2000,164,1: | 1 |
| 15 | Classes of analytic functions associated with the Choi-Saigo-Srivastava operator显示文摘 | Sókol J | 2006 | J Math Anal Appl2006,318,: | 1 |
| 16 | ovarian stimulation in vitro fertilization with or without the long gonadotropin-releasing homone agonist protocol effect on cycle duration and outcome显示文摘 | Bcloosesky g Kols Lightman A | 2008 | Fertii steril2008,74,: | 1 |
| 17 | Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix显示文摘 | Kolácná L Bakesová J Varga F | 2007 | Physiol Res2007,56,: | 1 |
| 18 | Themo - Mechanical Model of Spindles 显示文摘 | Holkup T Cao H Kol P | 2010 | CIRP Annals - Manufacturing Technology2010,59,1: | 1 |
| 19 | Neuroprotective effect of granulocyte colony-stimulating factor after focal cerebral ischemia显示文摘 | Sch?bitz WR Kol mar R Schwaninger M | | 0,,03: | 1 |
| 20 | Rapid and efficient homing of human CD34(+)CD38(-/low)CXCR4(+) stem and progenitor cells to the bone marrow and spleen of NOD/SCID and NOD/SCID/B2m(nul) mice显示文摘 | Kol et O Spiegel A Peled A | 2001 | {H}Blood2001,,10: | 1 |