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| 1 | Catabolism of Branched Chain Amino Acids Supports Respiration but Not Volatile Synthesis in Tomato Fruits显示文摘分支链氨基酸 transaminases (BCAT ) 在分支链氨基酸白氨酸,异白氨酸,和缬氨酸的新陈代谢有一个关键角色。这些酶催化合成的最后步和这些氨基酸的降级的起始的步。尽管在植物的分叉的链氨基酸的 biosynthetic 小径广泛地被调查了,很多基因被描绘了,他们在植物的分解代谢完全还没被理解。我们以前在西红柿描绘了分叉的链氨基酸 transaminase 基因家庭,揭示 subcellular 本地化和六基因编码的酶的运动性质。这里,我们在水果开发期间检验了酶的可能的功能。我们进一步描绘了在分叉的链氨基酸 transaminases 的表示不同的转基因的植物 1 和 3,在线 overexpressing 任何一个 BCAT1 或 BCAT3 在在 BCAT1 和 volatiles 的层次缺乏的水果评估呼吸的率。我们测试的份量上经由喂实验的先锋和同位素,在水果 volatiles 的形成的分叉的链氨基酸和他们的相应 keto 酸的重要性。我们的结果不仅第一次在水果呼吸表明分叉的链氨基酸的重要性,而且揭示那 keto 酸,而非氨基酸,是为分叉的链风味 volatiles 的可能的先锋。 | Andrej Kochevenko Wagner L. Araujo Gregory S. Maloney Denise M. Tieman Phuc Thi Do Mark G. Taylor Harry J. Klee Alisdair R. Fernie | 2012 | Molecular Plant2012，5，2： | 4 |
| 2 | Variations in length of stay among survived very preterm infants admitted to Chinese neonatal intensive care units显示文摘Background This study aimed to describe length of stay(LOS)to discharge and site variations among very preterm infants(VPIs)admitted to 57 Chinese neonatal intensive care units(NICUs)and to investigate factors associated with LOS for VPIs.Methods This retrospective multicenter cohort study enrolled all infants<32 weeks’gestation and admitted to 57 NICUs which had participated in the Chinese Neonatal Network,within 7 days after birth in 2019.Exclusion criteria included major congenital anomalies,NICU deaths,discharge against medical advice,transfer to non-participating hospitals,and missing discharge date.Two multivariable linear models were used to estimate the association of infant characteristics and LOS.Results A total of 6580 infants were included in our study.The overall median LOS was 46 days[interquartile range(IQR):35-60],and the median corrected gestational age at discharge was 36 weeks(IQR:35-38).LOS and corrected gestational age at discharge increased with decreasing gestational age.The median corrected gestational age at discharge for infants at 24 weeks,25 weeks,26 weeks,27-28 weeks,and 29-31 weeks were 41 weeks,39 weeks,38 weeks,37 weeks and 36 weeks,respectively.Significant site variation of LOS was identified with observed median LOS from 33 to 71 days in different hospitals.Conclusions The study provided concurrent estimates of LOS for VPIs which survived in Chinese NICUs that could be used as references for medical staff and parents.Large variation of LOS independent of infant characteristics existed,indicating variation of care practices requiring further investigation and quality improvement. | Min Zhang Yan-Chen Wang Jin-Xing Feng Ai-Zhen Yu Jing-Wei Huang Si-Yuan Jiang Xin-Yue Gu Jian-Hua Sun Yun Cao Wen-Hao Zhou Shoo KLee Li-Li Wang Rong Yin | 2022 | World Journal of Pediatrics2022，18，2： | 3 |
| 3 | The patients' perspective on physical symptoms after radiotherapy for cervical cancer 显示文摘 | Klee M Thranov I Machin D | 2000 | Gynecol Oncol2000，76，1： | 2 |
| 4 | Infrared thermography of the udder surface of dairy cattle: Characteristics, methods, and correlation with rectal temperature显示文摘 | Moritz Metzner Carola Sauter-Louis Andrea Seemueller Wolfram Petzl Wolfgang Klee | 2013 | The Veterinary Journal2013，，： | 2 |
| 5 | Sensory sacrifices when we mass-produce mass produce显示文摘Plant breeders have been extremely successful at driving genetic improvements in crops.However,‘improvements’are truly a question of perspective.Over the last one-hundred years most plant genetic innovations have been driven by industry demand.Larger fruits,heavier yields,uniformity,increased resistance to disease and better shipping quality are just a few of the traits that have ensured profits on the farm and affordable food for consumers.However,these milestones have come at the expense of sensory qualities,which have been sacrificed in exchange for practical production objectives.With a base of industry-sufficient genetics,today’s breeders can now turn to the consumer for guidance in defining critical desires.New approaches to plant breeding start with the analysis of consumer preferences,and then merge them with modern genomics and analytical chemistry tools.The result is the next generation of crops that meet supply chain demands while presenting improvements in flavor,nutrition,color,aroma and texture.This review analyzes the approach of consumer-assisted selection as it has been applied to tomato and strawberry,two complementary annual crops that have been intensively bred to meet industry expectations.Current breeding efforts start with the consumer,with the objective of reclaiming lost sensory qualities. | Kevin M Folta Harry J Klee | 2016 | Horticulture Research2016，3，1： | 2 |
| 6 | Quantitative Trait Loci Analysis Identifies a Prominent Gene Involved in the Production of Fatty Acid-Derived Flavor Volatiles in Tomato显示文摘到进在西红柿的类脂化合物新陈代谢的基因规定的获得卓见,我们进行了跟随水果果皮和茄属 pennellii 基因渗入的叶织物介绍的 lipidomic 的分析衬里的新陈代谢的特点 loci (mQTL )(IL ) 。提高为选择水果特定的 mQTL 印射分辨率,我们在独立地导出的 S 的一个子集介绍了类脂化合物。pennellii 回交生来的线,以及在 near-isogenic sub-IL ? 人口。我们识别了控制班 III 脂肪分解酵素家庭, LIP1 和 LIP2 的几类脂化合物,和二个成员的层次的通常认为的 lecithin:cholesterol acyltransferase,那与 diacylglycerols (DAG ) 和 triacylglycerols (标签) 的减少的层次被联系。这个班的脂肪分解酵素从 acylglycerols 的甘油脊梁劈开丰满的酸。释放的丰满的酸用作风味 volatiles 的先锋。我们证明那 LIP1 表情与丰满的导出酸的不稳定的层次相关。我们进一步用转基因的植物在标签和 DAG 故障和不稳定的合成证实 LIP1 的功能。一起拿,我们的学习广泛地在西红柿描绘了脂性的混合物的基因建筑学并且在分子的水平证明从甘油脊梁的免费的丰满的酸的版本能在下游地不稳定的合成上有主要影响。 | Karolina Garbowicz Zhongyuan Liu Saleh Alseekh Denise Tieman Mark Taylor Anastasiya Kuhalskaya Itai Ofner Dani Zamir Harry J. Klee Alisdair R. Fernie Yariv Brotman | 2018 | Molecular Plant2018，11，9： | 2 |
| 7 | Status of the neonatal follow‑up system in China:survey and analysis显示文摘Background There is little information about neonatal follow-up programs(NFUPs)in China.This study aimed to conduct a survey of hospitals participating in the Chinese Neonatal Network(CHNN)to determine the status of NFUPs,including resources available,criteria for enrollment,neurodevelopmental assessments,and duration of follow-up.Methods We conducted a descriptive study using an online survey of all 72 hospitals participating in CHNN in 2020.The survey included 15 questions that were developed based on the current literature and investigators’knowledge about followup practices in China.Results Sixty-four(89%)of the 72 hospitals responded to the survey,with an even distribution of children’s(31%),maternity(33%)and general(36%)hospitals.All but one(98%)hospital had NFUPs,with 44(70%)being established after 2010.Eligibility criteria for follow-up were variable,but common criteria included very preterm infants<32 weeks or<2000 g birth weight(100%),small for gestational age(97%),hypoxic ischemic encephalopathy(98%)and postsurgery(90%).The average follow-up rate was 70%(range:7.5%–100%).Only 12%of hospitals followed up with patients for more than 24 months.There was significant variation in neurodevelopmental assessments,follow-up schedule,composition of staff,and clinic facilities and resources.None of the staff had received formal training,and only four hospitals had sent staff to foreign hospitals as observers.Conclusions There is significant variation in eligibility criteria,duration of follow-up,types of assessments,staffing,training and facilities available.Coordination and standardization are urgently needed. | Qi Zhou Yun Cao Lan Zhang Nurya Erejep Wen‑Long Xiu Jing‑Yun Shi Rui Cheng Wen‑Hao Zhou Shoo KLee | 2023 | World Journal of Pediatrics2023，19，11： | 2 |
| 8 | Widespread dispersal of the microsporidian Nosema ceranae , an emergent pathogen of the western honey bee, Apis mellifera显示文摘 | Julia Klee Andrea M. Besana Elke Genersch Sebastian Gisder Antonio Nanetti Dinh Quyet Tam Tong Xuan Chinh Francisco Puerta José Maria Ruz Per Kryger Dejair Message Fani Hatjina Seppo Korpela Ingemar Fries Robert J. Paxton | 2007 | Journal of Invertebrate Pathology2007，，1： | 2 |
| 9 | Life after radiotherapy: the psychological and social effects experienced by women treated for advanced stages of cervical cancer 显示文摘 | Klee M Thranov I Machin D | 2000 | Gynecol Oncol2000，76，1： | 2 |
| 10 | Sol-gel and mod procuring of layered perovskite films显示文摘 | Klee M Mackens U | 1996 | Integrated Ferroelectrics1996，12，1： | 1 |
| 11 | Regulation of migrating motor complexes by motilin and pancreatic polypeptide in human infants显示文摘 | Jadcheda SR Klee G Berseth CL | 1997 | Pediatr Res1997，42，3： | 1 |
| 12 | Free,complexed and total serum prostate specific antigen:The establishment of appropriate reference ranges for their concentration and rations显示文摘 | Oesterling J E Jacobsen S J Klee G | 1999 | Urol1999，154，： | 1 |
| 13 | Bcl-X(L) specifically activates Bak to induce swelling and restructuring of the endoplasmic reticulum显示文摘 | Klee M Pimentel-Muinos FX | 2005 | J Cell Biol2005，168，5： | 1 |
| 14 | Inactivation ofauxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of Pseudomonas savastanoi显示文摘 | Romano C P Hein M B Klee H J | | 0，，03： | 1 |
| 15 | Electrospinning with dual col- lection tings显示文摘 | Dalton P D Klee D Mller M | 2005 | Polymer2005，46，3： | 1 |
| 16 | Free, complexed and total serum prostate specific antigen:the establishment ofappropriate reference ranges for their concentrations and ratios显示文摘 | Oesterling JE Jacobsen S J Klee GG | 1995 | J Urol1995，154，3： | 1 |
| 17 | Longitudinal study of sexual function and vaginal changesafter radiotherapy for cervical cancer 显示文摘 | JEUSEN P T GROENVOLD M KLEE M C | 2003 | Int J Radiat Oncol Biol Phys2003，56，4： | 1 |
| 18 | Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plant显示文摘 | KLEE H J WILKINSON J Q | 1991 | Plant Cell1991，3，： | 1 |
| 19 | Critical Reassessment and Model Development显示文摘 | HENNIG-THURAU T KLEE A A | 1997 | Psychology & Marketing1997，14，8： | 1 |
| 20 | Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase:Sequence analysis and manipulation to obtain glyphosate-tolerant plants显示文摘 | KLEE H MUSKOPF Y GASSER C | 1987 | Molecular and General Genetics1987，210，3： | 1 |
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