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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Prediction of Dynamic and Aerodynamic Characteristics of the Centrifugal Fan with Forward Curved Blades显示文摘The main aim of this paper is determine the centrifugal fan with forward curved blades aerodynamic characteristics based on numerical modeling. Three variants of geometry were investigated. The first, basic 'A' variant contains 12 blades. The geometry of second 'B' variant contains 12 blades and 12 semi-blades with optimal length[1]. The third, control variant 'C' contains 24 blades without semi-blades. Numerical calculations were performed by CFD Ansys. Another aim of this paper is to compare results of the numerical simulation with results of approximate numerical procedure. Applied approximate numerical procedure [2] is designated to determine characteristics of the turbulent flow in the bladed space of a centrifugal-flow fan impeller. This numerical method is an extension of the hydro-dynamical cascade theory for incompressible and inviscid fluid flow. Paper also partially compares results from the numerical simulation and results from the experimental investigation. Acoustic phenomena observed during experiment, during numerical simulation manifested as deterioration of the calculation stability, residuals oscillation and thus also as a flow field oscillation. Pressure pulsations are evaluated by using frequency analysis for each variant and working condition. | Jií Polansky László Kalmár Roman Gápár | 2013 | Journal of Thermal Science2013，22，6： | 2 |
| 3 | Favorable response to high-dose infliximab for refractory childhood uveitis 显示文摘 | Kalm P Weiss M Imundo LF Levy DM | 2006 | Oph- thalmology2006，113，5： | 1 |
| 4 | One-year lowdose erythromycin treatment of persistent chronic sinusitis after sinus surgery:clinical outcome and effects on mucociliary parameters and nasal nitric oxide显示文摘 | Cervin Anders Kalm O Sandkull P | | Otolaryngol Head Neck Surg0，126，5： | 1 |
| 5 | Clinical pathways:experiences,expectations,and perspectives显示文摘 | Lohfert C Kalmár P | 2006 | Intemist (Berl)2006，47，67： | 1 |
| 6 | Clinical pathways:experiences,expectations,and perspectives显示文摘 | Lohfert C Kalmár P | 2006 | Der Internist2006，47，67： | 1 |
| 7 | One-year low-dose erythromycin treatment of persistent chronic sinusitis after sinus surgery:clinical outcome and effects on mucociliary parameters and nasal nitric oxide显示文摘 | Cervin A Kalm O Sandkull P | 2002 | Otolaryngol Head Neck Surg2002，126，5： | 1 |
| 8 | Eustachian tube function and tympatic membrane findings after chronic secretory otitis raedia显示文摘 | Ryding M White P Kalm O | 2004 | Int J Pediatr Otorhinolaryngol2004，68，2： | 1 |
| 9 | Course and long-term outcome ofrefractory secretory otitis media显示文摘 | Ryding M White P Kalm O | 2005 | J laryngol Otol2005，119，2： | 1 |
| 10 | One-year low-dose erythro-mycin treatment of persistent chronic sinusitis after sinus surgery: clinical outcome and effects on mucociliary parameters and nasal nitric oxide显示文摘 | Cervin A Kalm O Sandkull P | 2002 | Otolaryngol Head Neck Surg2002，126，5： | 1 |
| 11 | Eustachian tube function and tympatic membrane findings after chronic secretory otitis media显示文摘 | White P kalm O | 2004 | Int J Pediatr Otorhinolaryngol2004，68，2： | 1 |
| 12 | Eustachian tube function and tympatic membrane findings after chronic secretory otitis media显示文摘 | Ryding M White P Kalm O | 2004 | Int J Pediatr Otorhinolaryngol2004，68，2： | 1 |
| 13 | Course and long-term outcome of refractory secretory otitis media显示文摘 | Ryding M White P Kalm O | 2005 | J laryngolotol2005，119，2： | 1 |
| 14 | Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC463显示文摘 | Jadhav J P Parshetti G K Kalme S D | 2007 | Chemosphere2007，68，2： | 1 |
| 15 | Eustachian tube function and tympanic membrane findings after chronic secretory otitis media显示文摘 | Ryding M White P Kalm O | 2004 | Int J Pediatr O- torhinolaryngol2004，68，2： | 1 |
| 16 | Oneyear low-dose erythromycin treatment of persistent chronic sinusitis after sinus surgery:clinical outcome and effects on mucociliary parameters and nasal nitric oxide显示文摘 | CERVIN A KALM O SANDKULL P | 2002 | Otolaryngol Head Neck Surg2002，126，： | 1 |
| 17 | Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463显示文摘 | Jadhav J P Parshetti G K Kalme S D | | 0，，02： | 1 |
| 18 | Biodegradation of methyl red by Galactomyces geotrichum MTCC 1360显示文摘 | Jadhav S U Kalme S D Govindwar S P | | 0，，02： | 1 |
| 19 | Course and long term outcome of'refractory'secretory otitis media显示文摘 | Ryding M White P Kalm O | 2005 | The Journal of Laryn- gology and Otology2005，119，： | 1 |
| 20 | One-year low-dose e-rythromycin treatment of persistent chronic sinusitis aftersinus surgery:clinical outcome and effects on mucociliaryparameters and nasal nitric oxide 显示文摘 | Cervin A Kalm 0 Sandkull P | 2002 | Otolaryngol HeadNeck Surg2002，126，5： | 1 |
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