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| 1 | Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma显示文摘AIM To explore the effect and mechanism ofgastrin and its antagonists proglumide and somatostatin on coIorectal carcinoma and their clinical significance.METHODS A model of transplanted human colonic carcinoma was established from SW480cell line in gymnomouse body. The volume andweight of transplanted carcinoma was observedunder the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content ot carcinoma cell was determined by redioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viabIe cells was determined by MTT colorimetric analysis, lP3 content was determined by radioimmunoassay, Ca2+ concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc,C-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immunocytochemistry SP method. Argyrophilianucleolar organizer regions was determined withargyrophilia stain.RESULTS The volume, weight, cAMP, DNAand protein content in carcinoma cell, cellamount and proliferation index of S and G2Mphase in PG group were all significantly higher than those of control group. When PG was at theconcentration of 25 mg/ L, the amount of viablecells, lP3 content and Ca2+ concentration in celland membrane PKC activity in PG group weresigniticantly higher than those in control group;when PGL was at a concentration ot 32 mg/L,they dropped to the lowest level in PG (25 mg/L)+ PGL group, but without significant differencefrom the control group. The positive expression rate of gastrin, c-myc, c-fos and rasp21 in carcinoma tissue was 39 .6%, 54 .2%, 47. 9% and54 .2% resPectively and significantly higher than that in mucosa 3 cm and 6 cm adjacent tocarcinoma tissue and normal colorectal mucosa.The positive expression rate of gastrin of highlydifferentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous adenocarcinoma groups. The AgNORs count of carcinoma tissue was significantly higher than that in mucosa 3 cm and 6 cm adjacent to carcinoma tissue and normal colorectal mucosa; and the positive expression of c-myc and c-fos and the AgNORs count in gastrin-positive group was significantly higher than those in gastrin-negative group.CONCLUSION Pentagastrin has a promoting effect on the growth of transplanted human colonic carcinoma from SW480 cell line. PGL hasno obvious effect on the growth of human colonic carcinoma SW480 cell line, but couldinhibit the growth-promoting effect of PG on transplanted carcinoma. Somatostatin can not only inhibit the growth of transplanted human colonic carcinoma from SW480 cell line directlybut also depress the growth-promoting effect ofgastrin on the transplanted carcinoma. Somecolorectal carcinoma cells can produce and secrete gastrin through autocrine, highly-differentiated adenocarcinoma express the highest level gastrin. Endogenous gastrin can stimulate the cell division and proliferation of carcinoma cell and promote the growth of colorectal carcinoma regulating the expression of oncogene omyc, c-fos. Our study has provided experimental basis for the adjuvant treatment using gastrin antagonist such as PGL,somatostatin of patients with colorectalcarcinoma. | He SW Shen KQ He YJ Xie B Zhao YM | 1999 | World Journal of Gastroenterology1999，5，5： | 20 |
| 2 | 一步诊断丙型肝炎病毒血症的高特异性和敏感性的方法显示文摘【据《Hepatology》2016年6月报道】题:一步诊断丙型肝炎病毒血症的高特异性和敏感性的方法:HCV抗原酶联免疫测定法(作者Hu KQ等)目前诊断丙型肝炎标准的方法包括两个连续的步骤:首先是丙型肝炎抗体的筛查,然后是丙型肝炎的核酸定量确诊病毒血症性HCV(V-HCV)感染。丙型肝炎核心抗原检测为一步诊断丙型肝炎提供了可能性,然而, | Hu KQ 刘苗霞 王中峰 | 2016 | 临床肝胆病杂志2016，32，8： | 2 |
| 3 | Immunization of RANTES expression plasmid with a DNA vaccine enhances HIV-1-specific immunity显示文摘 | Lu Y Hamajima K | 1999 | Clin Immunol1999，92，1： | 1 |
| 4 | Effect of the cyclin-dependent kinases inhibitor p27 on resistance of ovarian cancer multicellular spheroids to anticancer chemotherapy显示文摘 | Xing H Wang SX Hu KQ | 2005 | J Cancer Res Clin Oncol2005，131，5： | 1 |
| 5 | Influence of chronic fluorosis on membrane lipids in rat brain 显示文摘 | Guan ZZ Wang YN Xiao KQ | 1998 | Neurotoxicol Teratol1998，20，5： | 1 |
| 6 | Hyperfibrinolytic activity in hospitalized cirrhotic patients in a referral liver unit 显示文摘 | Hu KQ Yu AS Tiyyagura L | 2001 | Am J Gastroenterol2001，96，5： | 1 |
| 7 | Sengstaken-Blakenmre tubes treat for the Mallory- Weiss syndrome 显示文摘 | Xu FQ Kang KQ | 1995 | Chongqing Medical Journal1995，24，2： | 1 |
| 8 | Serum cholesterol and statin use predict virological response to peginterferon and ribavirin therapy显示文摘 | Harrison SA Rossaro L Hu KQ | | 0，，3： | 1 |
| 9 | The modulatory effects of garlic oil on hepatic cytochrome P450s in mice显示文摘 | Zeng T Zhang CL Xie KQ | 2009 | Human and Experimental Toxicology2009，28，12： | 1 |
| 10 | In vitro chemosensitivity in breast cancer using ATP-tumor chemosensitivity assay显示文摘 | Qi CJ Zhu YL Qian KQ | 2009 | Arch Pharm Res2009，32，12： | 1 |
| 11 | Management of ascites显示文摘 | Yu AS Hu KQ | 2001 | Clin Liver Dis2001，5，2： | 1 |
| 12 | Review of the female Duroc/Yorkshire pig model of human fibroproliferative scarring显示文摘 | Zhu KQ Carrougher GJ Gibran NS | 2007 | Wound Repair Regen2007，，： | 1 |
| 13 | Procyanidin dimer B2 suppresses the expression of cyclooxygenase-2 in endotoxin-treated monocytic ceils 显示文摘 | Zhang WY Liu HQ Xie KQ | 2006 | Biochem Biophys Res Commun2006，345，： | 1 |
| 14 | Smae/DIABLO and cy- tochrome care released from mitochondria through a simi- lar mechanism during UV-induced apoptosis显示文摘 | Zhou LL Zhou LY Luo KQ | 2005 | Apoptosis2005，10，2： | 1 |
| 15 | Update on rescue therapies in patients with lamivudine-resistant chronic hepatitis B显示文摘 | Chao DC Hu KQ | 2013 | Drug Des Devel Ther2013，7，： | 1 |
| 16 | A novel frameshift mutation of epithelial sodium channel heta-subunit leads to Liddle syndrome in an isolated case显示文摘 | Yang KQ Lu CX Xiao Y | 2015 | Clin Endocrinol (Oxf)2015，82，4： | 1 |
| 17 | A model to predict 3-month mortality risk of acute-on-chronic hepatitis B liv-er failure using artificial neural network 显示文摘 | Zheng MH Shi KQ Lin XF | 2013 | J Viral Hep-at2013，20，4： | 1 |
| 18 | Viral,host and interferon-related factors modulating the effect of interferon therapy for hepatitis C virus infection显示文摘 | Hu KQ Vierling JM Redeker AG | 2001 | J Viral Hepat2001，8，1： | 1 |
| 19 | Influence of chronic Fluorosison membrane lipids in rat brain显示文摘 | Guan ZZ Wang YN Xiao KQ | 1998 | Neurotoxicol Teratol1998，20，3： | 1 |
| 20 | Smac/DIABLO and cyto- chrome c are released from mitochondria through a similar mechanism during UV-induced apoptosis显示文摘 | Zhou LL Zhou LY Luo KQ | 2005 | Apoptosis2005，10，2： | 1 |
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