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| 1 | Positioning accuracy assessment for the 4GEO/5IGSO/2MEO constellation of COMPASS显示文摘Determined to become a new member of the well-established GNSS family,COMPASS(or BeiDou-2) is developing its capabilities to provide high accuracy positioning services.Two positioning modes are investigated in this study to assess the positioning accuracy of COMPASS' 4GEO/5IGSO/2MEO constellation.Precise Point Positioning(PPP) for geodetic users and real-time positioning for common navigation users are utilized.To evaluate PPP accuracy,coordinate time series repeatability and discrepancies with GPS' precise positioning are computed.Experiments show that COMPASS PPP repeatability for the east,north and up components of a receiver within China's Mainland is better than 2 cm,2 cm and 5 cm,respectively.Apparent systematic offsets of several centimeters exist between COMPASS precise positioning and GPS precise positioning,indicating errors remaining in the treatments of COMPASS measurement and dynamic models and reference frame differences existing between two systems.For common positioning users,COMPASS provides both open and authorized services with rapid differential corrections and integrity information available to authorized users.Our assessment shows that in open service positioning accuracy of dual-frequency and single-frequency users is about 5 m and 6 m(RMS),respectively,which may be improved to about 3 m and 4 m(RMS) with the addition of differential corrections.Less accurate Signal In Space User Ranging Error(SIS URE) and Geometric Dilution of Precision(GDOP) contribute to the relatively inferior accuracy of COMPASS as compared to GPS.Since the deployment of the remaining 1 GEO and 2 MEO is not able to significantly improve GDOP,the performance gap could only be overcome either by the use of differential corrections or improvement of the SIS URE,or both. | ZHOU ShanShi CAO YueLing ZHOU JianHua HU XiaoGong TANG ChengPan LIU Li GUO Rui HE Feng CHEN JunPing WU Bin | 2012 | Science China(Physics,Mechanics & Astronomy)2012，55，12： | 31 |
| 2 | Mutation profile and its correlation with clinicopathology in Chinese hepatocellular carcinoma patients显示文摘Background:Hepatocellular carcinoma(HCC)is one of the most common causes of cancer worldwide.Although many studies have focused on oncogene characteristics,the genomic landscape of Chinese HCC patients has not been fully clarified.Methods:A total of 165 HCC patients,including 146 males and 19 females,were enrolled.The median age was 55 years(range,27-78 years).Corresponding clinical and pathological information was collected for further analysis.A total of 168 tumor tissues from these patients were selected for next-generation sequencing(NGS)-based 450 panel gene sequencing.Genomic alterations including single nucleotide variations(SNV),short and long insertions and deletions(InDels),copy number variations,and gene rearrangements were analyzed.Tumor mutational burden(TMB)was measured by an algorithm developed in-house.The top quartile of HCC was classified as TMB high.Results:A total of 1,004 genomic alterations were detected from 258 genes in 168 HCC tissues.TMB values were identified in 160 HCC specimens,with a median TMB of 5.4 Muts/Mb(range,0-28.4 Muts/Mb)and a 75%TMB of 7.7 Muts/Mb.The most commonly mutated genes were TP53,TERT,CTNNB1,AXIN1,RB1,TSC2,CCND1,ARID1A,and FGF19.SNV was the most common mutation type and C:G>T:A and guanine transformation were the most common SNVs.Compared to wild-type patients,the proportion of Edmondson grade III-IV and microvascular invasion was significantly higher in TP53 mutated patients(P<0.05).The proportion of tumors invading the hepatic capsule was significantly higher in TERT mutated patients(P<0.05).The proportion of Edmondson grade I-II,alpha fetoprotein(AFP)<25μmg/L,and those without a history of hepatitis B was significantly higher in CTNNB1 mutated patients(P<0.05).CTNNB1 mutations were associated with TMB high in HCC patients(P<0.05).Based on correlation analysis,the mutation of TP53 was independently correlated with microvascular invasion(P=0.002,OR=3.096)and Edmondson grade III-IV(P=0.008,OR=2.613).The mutation of TERT was independently correlated with tumor invasion of the liver capsule(P=0.001,OR=3.030),and the mutation of CTNNB1 was independently correlated with AFP(<25μmg/L)(P=0.009,OR=3.414).Conclusions:The most frequently mutated genes of HCC patients in China were TP53,TERT,and CTNNB1,which mainly lead to the occurrence and development of HCC by regulating the P53 pathway,Wnt pathway,and telomere repair pathway.There were more patients with microvascular invasion and Edmondson III-IV grade in TP53 mutated patients and more patients with hepatic capsule invasion in TERT mutated patients,while in CTNNB1 mutated patients,there were more patients with Edmondson I-II grade,AFP<25μmg/L,and a non-hepatitis B background.Also,the TMB values were significantly higher in CTNNB1 mutated patients than in wild type patients. | Shuo Wang Huasheng Shi Tao Liu Manjiang Li Sanshun Zhou Xuan Qiu Zusen Wang Weiyu Hu Weidong Guo Xiaoqian Chen Honglin Guo Xiaoliang Shi Junping Shi Yunjin Zang Jingyu Cao Liqun Wu | 2021 | Hepatobiliary Surgery and Nutrition2021，10，2： | 8 |
| 3 | RNA interference targeting CD147 inhibits metastasis and invasion of human breast cancer MCF-7 cells by downregulating MIVIP-9/VEGF expression显示文摘 | Fang Li Junping Zhang Jiqiang Guo Yuan Jia Yaping Han Zhuanhua Wang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，7： | 7 |
| 4 | Determining antimicrobial resistance profiles and identifying novel mutations of Neisseria gonorrhoeae genomes obtained by multiplexed MinION sequencing显示文摘Gonorrhea is one of the most common sexually transmitted diseases worldwide. To cure infection and prevent transmission,timely and appropriate antimicrobial therapy is necessary. Unfortunately, Neisseria gonorrhoeae, the etiological agent of gonorrhea, has acquired nearly all known mechanisms of antimicrobial resistance(AMR), thereby compromising the efficacy of antimicrobial therapy. Treatment failure resulting from AMR has become a global public health concern. Whole-genome sequencing is an effective method to determine the AMR characteristics of N. gonorrhoeae. Compared with next-generation sequencing, the MinION sequencer(Oxford Nanopore Technologies(ONT)) has the advantages of long read length and portability. Based on a pilot study using MinION to sequence the genome of N. gonorrhoeae, we optimized the workflow of sequencing and data analysis in the current study. Here we sequenced nine isolates within one flow cell using a multiplexed sequencing strategy. After hybrid assembly with Illumina reads, nine integral circular chromosomes were obtained. By using the online tool Pathogenwatch and a BLAST-based workflow, we acquired complete AMR profiles related to seven classes of antibiotics. We also evaluated the performance of ONT-only assemblies. Most AMR determinants identified by ONT-only assemblies were the same as those identified by hybrid assemblies. Moreover, one of the nine assemblies indicated a potentially novel antimicrobial-related mutation located in mtrR which results in a frame-shift, premature stop codon, and truncated peptide.In addition, this is the first study using the MinION sequencer to obtain complete genome sequences of N. gonorrhoeae strains which are epidemic in China. This study shows that complete genome sequences and antimicrobial characteristics of N.gonorrhoeae can be obtained using the MinION sequencer in a simple and cost-effective manner, with hardly any knowledge of bioinformatics required. More importantly, this strategy provides us with a potential approach to discover new AMR determinants. | Chi Zhang Feng Wang Cansheng Zhu Leshan Xiu Yamei Li Li Li Bo Liu Yizhun Li Yaling Zeng Boyang Guo Junping Peng | 2020 | Science China(Life Sciences)2020，63，7： | 2 |
| 5 | Investigation on Infection of PCV2 in Taizhou Areas of Jiangsu Province显示文摘In order to investigate infection and harm extent of swine porcine circovirus 2( PCV2) in pigs in Taizhou of Jiangsu,and to control the prevalence of PCV2 in Taizhou areas. A total of 116 PCV2 clinical samples came from six different cities( districts) from 2011 to 2012 were tested in etiologic by the method of PCR. The test results showed that the total positive rate of PCV2 clinical samples was 53. 45%,and PCV2 existed in all the six places. The positive rate of the scale pig farms was 48. 33%,the positive rate of free-range farmers was 58. 93%. Thus,PCV2 was prevalent in pigs in Taizhou areas,and which should be paid more attention to. | Cao Junping Guo Guangfu Wu Yantao Lu Guiping Zhu Aiping | 2015 | Animal Husbandry and Feed Science2015，7，4： | 2 |
| 6 | Axisymmetric-fold combination laser resonator 显示文摘 | Yude Li Jinglun Liu Mei Chen Junping Guo | 2005 | Optical Engineering v44 n62005，64，204： | 1 |
| 7 | The Temporal Relation between Cardiomyopathy and LBBB and Response to Cardiac Resynchronization Therapy:Case Series and Literature Review显示文摘Background:Left bundle branch block(LBBB)-induced cardiomyopathy has been proposed,but the association between LBBB and cardiac resynchronization therapy(CRT)response remains unclear and practical criteria for selecting CRT candidates are needed.Methods:One hundred and seventeen consecutive heart failure patients were reviewed,24 of whom received CRT.Only two patients had a clear temporal relation between cardiomyopathy and LBBB.Results:Compared with the patient with“cardiomyopathy-induced LBBB,”the patient with“LBBB-induced cardiomyopathy”had higher left ventricular(LV)wall thickness,higher LV wall thickening rate,higher peak circumferential strain,and longer peak circumferential strain delay.The LV deformation patterns in the two patients were obviously distinct on cardiovascular magnetic resonance tissue tracking.During follow-up,the patient with LBBB-induced cardiomyopathy had a good response to CRT(LV ejection fraction 23 before CRT vs.30%at 6 months vs.29 at 12 months vs.32%at 18 months;LV end-diastolic diameter 77 mm before CRT vs.66 mm at 6 months vs.62 mm at 12 months vs.63 mm at 18 months),and the other patient had no response to CRT(LV ejection fraction 29 before CRT vs.29%at 6 months vs.26 at 12 months vs.22%at 24 months;LV end-diastolic diameter 85 mm before CRT vs.88 mm at 6 months vs.85 mm at 12 months vs.84 mm at 24 months).Conclusion:The temporal relation between cardiomyopathy and LBBB could be a determinant for CRT response.Cardiovascular magnetic resonance tissue tracking may be a useful tool to identify the chronological order and a principal consideration for selecting candidates for CRT.Larger prospective clinical trials are needed to study the prevalence of,time course of,and risk factors for LBBB-induced cardiomyopathy. | Sanshuai Chang Yi He Hui Wang Fei Guo Qiang Lv Junping Kang Rong Bai Xiaohui Liu Xin Du Changsheng Ma Jianzeng Dong | 2020 | Cardiovascular Innovations and Applications2020，，1： | 1 |
| 8 | Isolation and Identification of a Porcine Pseudorabies Virus Strain in Taizhou City显示文摘In this study,the liver,kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District,Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus,which was named TAIZ130417. The growth titer of the isolated virus reached 108. 12 TCID50/ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies. | Guangfu GUO Aiping ZHU Junping CAO Cailian JIN Lihong DAI | 2018 | Agricultural Biotechnology2018，7，5： | 1 |
| 9 | Research on near field distribution of axisymmetric folded-combined CO2 laser显示文摘 | Yude Li Junping Guo | 2006 | Optical Engineering2006，45，7： | 1 |
| 10 | Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon显示文摘Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate synthase 4(Cit ACS4), expressed specifically in carpel primordia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism(SNPs) and one InDel identified in the coding region of Cit ACS4, the C364 W mutation located in the conserved box 6 was cosegregated with andromonoecy. Enzymatic analyses showed that the C364 W mutation caused a reduced activity in Cit ACS4. We believe that the reduced Cit ACS4 activity may hamper the programmed cell death in stamen primordia, leading to the formation of hermaphroditic flowers. | Gaojie Ji Jie Zhang Haiying Zhang Honghe Sun Guoyi Gong Jianting Shi Shouwei Tian Shaogui Guo Yi Ren Huolin Shen Junping Gao Yong Xu | 2016 | Journal of Integrative Plant Biology2016，58，9： | 1 |
| 11 | Self-reproducing ray-cluster and misalignment of gas laser resonator having a coaxial discharge chamber显示文摘 | Li Yude Chen Mei Guo Junping | 2009 | Optics and Laser Technology2009，41，3： | 1 |
| 12 | An adaptive Kalman filtering based state of charge combined estimator for electric vehicle battery pack 显示文摘 | Wang Junping Guo Jingang Ding Lei | 2009 | Energy Conversion and Management2009，50，12： | 1 |
| 13 | Research on near-field distributions of axisymmetric folded-combined CO2 laser显示文摘 | Liu Jinglun Li Yude Guo Junping | 2006 | Opt Eng2006，45，07： | 1 |
| 14 | Characterization of the Convoluted 3D Internetallic Phases in a Recycled Al Alloy by Synchrotron X-ray Tomography and Machine Learning显示文摘Fe-rich intermetallic phases in recycled Al alloys often exhibit complex and 3D convoluted structures and morphologies.They are the common detrimental intermetallic phases to the mechanical properties of recycled Al alloys.In this study,we used synchrotron X-ray tomography to study the true 3D morphologies of the Ferich phases,Al_(2)Cu phases and casting defects in an ascast Al-5Cu-1.5Fe-1Si alloy.Machine learning-based image processing approach was used to recognize and segment the diff erent phases in the 3D tomography image stacks.In the studied condition,theβ-Al_(9)Fe_(2)Si_(2)andω-Al_(7)Cu_(2)Fe are found to be the main Fe-rich intermetallic phases.Theβ-Al_(9)Fe_(2)Si_(2)phases exhibit a spatially connected 3D network structure and morphology which in turn control the 3D spatial distribution of the Al_(2)Cu phases and the shrinkage cavities.The Al_(3)Fe phases formed at the early stage of solidification aff ect to a large extent the structure and morphology of the subsequently formed Fe-rich intermetallic phases.The machine learning method has been demonstrated as a powerful tool for processing big datasets in multidimensional imaging-based materials characterization work. | Zhenhao Li Ling Qin Baisong Guo Junping Yuan Zhiguo Zhang Wei Li Jiawei Mi | 2022 | Acta Metallurgica Sinica(English Letters)2022，35，1： | 0 |
| 15 | MiR-30a-5p alleviates LPS-induced HPMEC injury through regulation of autophagy via Beclin-1显示文摘Background:Sepsis,a type of systemic disease,can impact nearly all organs,tissues and cells.Among them,endothelial cells are amongst thefirst to be affected and respond to the insult.In this study,we investigated the protective effects of microRNA-30a-5p(miR-30a-5p)on human pulmonary microvascular endothelial cells(HPMECs)treated with lipolysaccharide(LPS).Methods:An in vitro model of sepsis was established in HPMECs with the use of LPS.Transfecting with different tools(mimetic and inhibitor)to modify miR-30a-5p expression.Cell viability,proliferation and apoptosis were detected by the CCK-8 assay,the EdU kit andfluorescence staining,respectively.The autophagy-related protein and mRNA expression,the number of autophagosomes were separately examined through Western blot analysis,qPT-PCR and confocal microscopy.TargetScan and the luciferase reporter assays were used to probe target genes interacting with miR-30a-5p.Results:LPS caused a reduction in the viability and proliferation of HPMECs,as well as an elevation in the number of apoptotic cells.Subsequently,we observed that miR-30a-5p might play a role in preventing LPS-induced inhibition of cell damage and decreasing HPMEC apoptosis,suggesting the potential function of miR-30a-5p in this injury process.Finally,we confirmed that miR-30a-5p exerts its protective effect by regulating cell autophagy,possibly by targeting Beclin-1.Conclusion:Our study provided evidence that autophagy is a crucial aspect in the protective role of miR-30a-5p against LPS-induced HPMEC injury,identifying a promising target for sepsis-related endothelial cell injury. | RAN PAN JIAYAN MAO YUELIANG ZHENG WEI CHEN JUNPING GUO LIJUN WANG | 2024 | BIOCELL2024，48，3： | 0 |
| 16 | Isolation and Identification of Porcine Circovirus Type 2 in Taizhou显示文摘In September 2011,an infectious disease suspected to be postweaning multisystemic wasting syndrome( PMWS) broke out in some pig farm in Taizhou.The inguinal lymph node,liver and lung tissues were collected and grinded into tissue suspension. The suspension was subjected to PCR detection,and the positive product was sequenced. The suspension of positive samples was filtered with 0. 22 μm filter membrane,and the filtrate was inoculated onto PK15 cells. After five generations of blind passages,the cell viral liquid was collected and extracted for DNA,which was subjected to PCR detection and indirect immunofluorescence.The results showed that the isolate was porcine circovirus type 2( PCV2) and designated as TAIZ110926. The target sequence was committed to NCBI with a serial number: KF039888. | Guangfu GUO Aiping ZHU Junping CAO Cailian JIN Lihong DAI Hongwei TAO | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
| 17 | Tripterygium hypoglaucum extract ameliorates adjuvant-induced arthritis in mice through the gut microbiota显示文摘Traditionally,Tripterygium hypoglaucum(Levl.)Hutch(THH)are widely used in Chinese folk to treat rheumatoid arthritis(RA).This study aimed to investigate whether the anti-RA effect of THH is related with the gut microbiota.The main components of prepared THH extract were identified by HPLC-MS.C57BL/6 mice with adjuvant-induced arthritis(AIA)were treated with THH extract by gavage for one month.THH extract significantly alleviated swollen ankle,joint cavity exudation,and articular cartilage destruction in AIA mice.The mRNA and protein levels of inflammatory mediators in muscles and plasma indicated that THH extract attenuated inflammatory responses in the joint by blocking TLR4/MyD88/MAPK signaling pathways.THH extract remarkably restored the dysbiosis of the gut microbiota in AIA mice,featuring the increases of Bifidobacterium,Akkermansia,and Lactobacillus and the decreases of Butyricimonas,Parabacteroides,and Anaeroplasma.Furthermore,the altered bacteria were closely correlated with physiological indices and drove metabolic changes of the intestinal microbiota.In addition,antibiotic-induced pseudo germ-free mice were employed to verify the role of the intestinal flora.Strikingly,THH treatment failed to ameliorate the arthritis symptoms and signaling pathways in pseudo germ-free mice,which validates the indispensable role of the intestinal flora.For the first time,we demonstrated that THH extract protects joint inflammation by manipulating the intestinal flora and regulating the TLR4/MyD88/MAPK signaling pathway.Therefore,THH extract may serve as a microbial modulator to recover RA in clincial practice.verRAinclincial practice. | HU Jianghui NI Jimin ZHENG Junping GUO Yanlei YANG Yong YE Cheng SUN Xiongjie XIA Hui LIU Yanju LIU Hongtao | 2023 | Chinese Journal of Natural Medicines2023，21，10： | 0 |
| 18 | Study on Rural Poverty Reduction Effect of Traffic Infrastructure显示文摘To systematically comb and explore the rural poverty reduction effect of traffic infrastructure,the author analyzed the mechanism,main channels and actual effect of rural traffic infrastructure on poverty reduction.It revealed that rural traffic infrastructure exerts positive or negative effect on poverty reduction directly or indirectly.The positive channel includes increasing non-agricultural employment opportunities,reducing costs for agricultural production,transportation and labor transfer,increasing availability of social service,and promoting adjustment of agricultural industrial structure and development of rural tourism resources.On the whole,traffic infrastructure has significant positive influence on rural poverty reduction.Finally,it summarized policy recommendations for building pro-poor traffic infrastructure,and came up with vacancy of related research field and future exploration direction. | Junping GUO Bin ZHANG Xiyan ZHANG | 2014 | Asian Agricultural Research2014，6，7： | 0 |
| 19 | Longitudinal proteomic investigation of cOVID-19 vaccination显示文摘Although the development of covID-19 vaccines has been a remarkable success,the heterogeneous individual antibody generation and decline over time are unknown and still hard to predict.In this study,blood samples were collected from 163 participants who next received two doses of an inactivated COvVID-19 vaccine(CoronaVac)at a 28-day interval.Using TMT-based proteomics,we identified 1,715 serum and 7,342 peripheral blood mononuclear cells(PBMCs)proteins.We proposed two sets of potential biomarkers(seven from serum,five from PBMCs)at baseline using machine learning,and predicted the individual seropositivity 57 days after vaccination(AUC=0.87).Based on the four PBMC's potential biomarkers,we predicted the antibody persistence until 180 days after vaccination(AUC=0.79).Our data highlighted characteristic hematological host responses,including altered lymphocyte migration regulation,neutrophil degranulation,and humoral immune response.This study proposed potential blood-derived protein biomarkers before vaccination for predicting heterogeneous antibody generation and decline after coVID-19 vaccination,shedding light on immunization mechanisms and individual booster shot planning. | Yingrui Wang Qianru Zhus Rui Sun Xiao Yi Lingling Huang Yifan Hu Weigang Ge Huanhuan Gao xinfu Ye Yu Song Li Shao Yantao Li Jie Li Tiannan Guo Junping Shi | 2023 | Protein & Cell2023，14，9： | 0 |
| 20 | The concordance between the evolutionary trend and the clinical manifestation of the two SARS-CoV-2 variants显示文摘Coronavirus disease 2019(COVID-19),the disease caused by infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has developed into a global pandemic that continues to pose an enormous threat to public health and the global economy.Since the first SARS-CoV-2 genome was released[1],thousands of genetic variants have been identified in SARS-CoV-2 strains isolated from worldwide patients. | Ben Hu Ran Liu Xiaolu Tang Yuchen Pan Ming Wang Yongqing Tong Guangming Ye Gaigai Shen Ruochen Ying Aisi Fu Di Li Wanxu Zhao Jing Peng Jie Guo Dong Men Xinmin Yao Yirong Wang Hong Zhang Zhihui Feng Junping Yu Liangjun Chen Zixin Deng Xuemei Lu Ya-Ping Zhang Yirong Li Bende Liu Lilei Yu Yan Li Jian Lu Tiangang Liu | 2021 | National Science Review2021，8，8： | 0 |
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