| 1 | Analyzing pH value, energy and phospholipid metabolism of various cerebral tumors and normal brain tissue with ^(31)P magnetic resonance spectroscopy显示文摘BACKGROUND: 31P magnetic resonance spectroscopy (31P MRS) can be used to non-injuredly and dynamicly detect various metabolites including phosphorus in organis and reflect changes of phospholipid metabolism and energy metabolism in tissue and pH value in cells. OBJECTIVE: To observe changes of pH value, phospholipid metabolism and energy metabolism of various cerebral tumors and normal brain tissue with 31P MRS. DESIGN: Semi-quantitative contrast observation. PARTICIPANTS: A total of 44 patients with cerebral tumor diagnosed with surgery operation were selected from the Department of Magnetic Resonance, Central South Hospital, Wuhan University from September 2004 to June 2006. All the subjects had complete 31P MRS data before steroid and operation. Among them, 16 patients had glioma of grade Ⅱ-Ⅲ, 12 spongioblastoma and 16 meningioma. The mean age was (45±6) years. Another 36 subjects without focus on cerebral MRI were regarded as normal group, including 19 males and 18 females, and the mean age was (41±4) years. Included subjects were consent. METHODS: Eclipse1.5T MRS (Philips Company) was used to collect wave spectrum; jMRUI(1.3) was used to analyze experimental data and calculate pH value in voxel and ratios of phosphocreatine (PCr)/inorganic phosphate (Pi), PCr/phosphodiesterase (PDE) and phosphomonoesterase (PME)/β-adenosine triphosphate (β-ATP) of various metabolites. 31P MRS results were compared with t test between tumor patients and normal subjects. MAIN OUTCOME MEASURES: Changes of phospholipid metabolism (PME/PDE), energy metabolism (PCr/ATP) and pH value of various cerebral tumors and normal brain tissues. RESULTS: A total of 44 cases with cerebral tumor and 36 normal subjects were involved in the final analysis. pH value and semi-quantitative measurements of normal brain tissues and various cerebral tumors: ① pH value at top occipital region and temple occipital region of normal brain tissue was 7.04±0.02; PCr/β-ATP was 1.51±0.03; PCr/Pi was 2.85±0.20; PDE/β-ATP was 2.70±0.40; PME/β-ATP was 1.01±0.03. ② Glioma of grade Ⅱ-Ⅲ: Tumor tissue was alkalized. The pH value was 7.09±0.01, which was higher than that in normal group (t=1.823, P < 0.05); PCr/β-ATP, PME/β-ATP and PCr/ Pi were 1.50±0.04, 1.04±0.07 and 2.49±0.21, respectively, which was close to that in normal group (P > 0.05); PDE/β-ATP was 1.73±0.20, which was lower than that in normal group (t=2.168, P < 0.05). ③ Spongioblastoma: Tumor tissue was alkalized. The pH value was 7.12±0.02, which was higher than that in normal group (t=2.170, P < 0.05); PCr/β-ATP and PCr/ Pi were 1.48±0.05 and 2.39±0.23, respectively, which was lower than that in normal group, but the difference was not significant (P > 0.05); PDE/β-ATP was 1.61±0.25, which was lower than that in normal group (t=3.582, P < 0.01); PME/β-ATP was 1.64±0.05, which was higher than that in normal group (t=3.930, P < 0.01). ④ Meningioma: Tumor tissue was alkalized. The pH value was 7.16±0.03, which was higher than that in normal group (t=2.978, P < 0.01); PCr/β-ATP, PCr/Pi and PDE/β-ATP were 0.46±0.04, 1.08±0.16 and 0.82±0.10, respectively, which was lower than that in normal group (t=3.132, 3.983, 3.985, P > 0.01); PME/β-ATP was 2.05±0.03, which was higher than that in normal group (t=3.007, P < 0.01). CONCLUSION: 31P MRS can provide informations about pH value of cerebral tumor, phospholipid metabolism and energy metabolism. Changes of pH value, PCr, PME and PDE can reflect metabolic characteristics of various cerebral tumors. | Wei Tan Guangyao Wu Junmo Sun | 2006 | Neural Regeneration Research2006，1，3： | 0 |
| 2 | Current state of stem cell research in non-human primates:an overview显示文摘The remarkable similarity between non-human primates(NHPs)and humans establishes them as essential models for understanding human biology and diseases,as well as for developing novel therapeutic strategies,thereby providing more comprehensive reference data for clinical treatment.Pluripotent stem cells such as embryonic stem cells and induced pluripotent stem cells provide unprecedented opportunities for cell therapies against intractable diseases and injuries.As continue to harness the potential of these biotechnological therapies,NHPs are increasingly being employed in preclinical trials,serving as a pivotal tool to evaluate the safety and efficacy of these interventions.Here,we review the recent advancements in the fundamental research of stem cells and the progress made in studies involving NHPs. | Junmo Wu Yuxi Shi Shanshan Yang Zengli Tang Zifan Li Zhuoyao Li Jiawei Zuo Weizhi Ji Yuyu Niu | 2023 | Medical Review2023，3，4： | 0 |
| 3 | Long-term in vivo chimeric cells tracking in non-human primate显示文摘Non-human primates(NHPs)are increasingly used in preclinical trials to test the safety and efficacy of biotech-nology therapies.Nonetheless,given the ethical issues and costs associated with this model,it would be highly advantageous to use NHP cellular models in clinical studies.However,developing and maintaining the naive state of primate pluripotent stem cells(PSCs)remains difficult as does in vivo detection of PSCs,thus limiting biotech-nology application in the cynomolgus monkey.Here,we report a chemically defined,xeno-free culture system for culturing and deriving monkey PSCs in vitro.The cells display global gene expression and genome-wide hypometh-ylation patterns distinct from monkey-primed cells.We also found expression of signaling pathways components that may increase the potential for chimera formation.Crucially for biomedical applications,we were also able to integrate bioluminescent reporter genes into monkey PsCs and track them in chimeric embryos in vivo and in vitro.The engineered cells retained embryonic and extra-embryonic developmental potential.Meanwhile,we generated a chimeric monkey carrying bioluminescent cells,which were able to track chimeric cells for more than 2 years in living animals.Our study could have broad utility in primate stem cell engineering and in utilizing chimeric monkey models forclinical studies. | Junmo Wu Yu Kang Xiang Luo Shaoxing Dai Yuxi Shi Zhuoyao Li Zengli Tang Zhenzhen Chen Ran Zhu Pengpeng Yang Zifan Li Hong Wang Xinglong Chen Ziyi Zhao Weizhi ji Yuyu Niu | 2024 | Protein & Cell2024，15，3： | 0 |
| 4 | Adsorption characteristics of Cd(Ⅱ) and Ni(Ⅱ) from aqueous solution using succinylated hay显示文摘An environmentally friendly organic biosorbent was fabricated using hay by succinylation. Metallic cation adsorption tests were performed using synthetic nickel(Ⅱ) and cadmium(Ⅱ) solutions to simulate heavy-metal recovery from aqueous solution. The adsorption efficiency was greater than 98% for both cadmium and nickel ions when the biosorbent concentration was 5.0 g/L and the initial metal concentrations were 50 mg/L. The surface of the biosorbent was characterized using Fourier transform infrared spectroscopy to investigate the changes in the surface functional groups. The functional groups changed according to the surface treatment, resulting in an effective biosorbent. The kinetics of the metals adsorption revealed that the reactions are pseudo-second order, and the adsorption isotherm well followed the Langmuir model. The maximum adsorption capacities predicted by the Langmuir model were 75.19 mg/g and 57.77 mg/g for cadmium and nickel, respectively. The fabricated biosorbent was regenerated using Na Cl multiple times, with 2.1% for Cd and 4.0% for Ni in adsorption capacity after three regeneration cycles. The proposed biosorbent can be a good alternative to resin or other chemical adsorbents for heavy-metal recovery in metallurgical processing or municipal water treatment. | Peijia Lin Jiajia Wu Junmo Ahn Jaeheon Lee | 2019 | International Journal of Minerals,Metallurgy and Materials2019，26，10： | 0 |
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