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| 1 | SARS-Coronavirus-2 Nsp13 Possesses NTPase and RNA Helicase Activities That Can Be Inhibited by Bismuth Salts显示文摘The ongoing outbreak of Coronavirus Disease 2019(COVID-19)has become a global public health emergency.SARScoronavirus-2(SARS-CoV-2),the causative pathogen of COVID-19,is a positive-sense single-stranded RNA virus belonging to the family Coronaviridae.For RNA viruses,virus-encoded RNA helicases have long been recognized to play pivotal roles during viral life cycles by facilitating the correct folding and replication of viral RNAs.Here,our studies show that SARS-CoV-2-encoded nonstructural protein 13(nsp13)possesses the nucleoside triphosphate hydrolase(NTPase)and RNA helicase activities that can hydrolyze all types of NTPs and unwind RNA helices dependently of the presence of NTP,and further characterize the biochemical characteristics of these two enzymatic activities associated with SARS-CoV-2 nsp13.Moreover,we found that some bismuth salts could effectively inhibit both the NTPase and RNA helicase activities of SARS-CoV-2 nsp13 in a dose-dependent manner.Thus,our findings demonstrate the NTPase and helicase activities of SARS-CoV-2 nsp13,which may play an important role in SARS-CoV-2 replication and serve as a target for antivirals. | Ting Shu Muhan Huang Di Wu Yujie Ren Xueyi Zhang Yang Han Jingfang Mu Ruibing Wang Yang Qiu Ding-Yu Zhang Xi Zhou | 2020 | Virologica Sinica2020，35，3： | 11 |
| 2 | The ORF3a protein of SARS-CoV-2 induces apoptosis in cells显示文摘Severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has caused the ongoing pandemic of Coronavirus Disease 2019.SARS-CoV-2 belongs to the genus Betacoronavirus of the Coronaviridae family,which includes SARS-CoV and Middle East respiratory syndrome coronavirus.1,2 Coronavirus-encoded accessory proteins play critical roles in virus–host interactions and the modulation of host immune responses,thereby contributing to coronaviral pathogenicity via different strategies.3 However,the functions of SARS-CoV-2-encoded accessory proteins are not well understood.Apoptosis is a predominant type of programmed cell death,and has been recognized as an important host antiviral defense mechanism that controls viral infection and regulates the inflammatory response.4,5 Previous studies have reported that the SARS-CoV-encoded accessory protein ORF3a can induce apoptosis in cells,6,7 leading to the question of whether SARS-CoV-2 ORF3a also has pro-apoptotic activity.Here,we investigated the potential apoptosis-inducing activity of SARS-CoV-2 ORF3a in different cell lines and compared the pro-apoptotic activities of SARS-CoV-2 ORF3a with those of SARS-CoV ORF3a using the same system. | Yujie Ren Ting Shu Di Wu Jingfang Mu Chong Wang Muhan Huang Yang Han Xue-Yi Zhang Wei Zhou Yang Qiu Xi Zhou | 2020 | Cellular & Molecular Immunology2020，17，8： | 11 |
| 3 | Plasma metabolomic and lipidomic alterations associated with COVID-19显示文摘The pandemic of the coronavirus disease 2019(COVID-19)has become a global public health crisis.The symptoms of COVID-19 range from mild to severe,but the physiological changes associated with COVID-19 are barely understood.In this study,we performed targeted metabolomic and lipidomic analyses of plasma from a cohort of patients with COVID-19 who had experienced different symptoms.We found that metabolite and lipid alterations exhibit apparent correlation with the course of disease in these patients,indicating that the development of COVID-19 affected their whole-body metabolism.In particular,malic acid of the TCA cycle and carbamoyl phosphate of the urea cycle result in altered energy metabolism and hepatic dysfunction,respectively.It should be noted that carbamoyl phosphate is profoundly down-regulated in patients who died compared with patients with mild symptoms.And,more importantly,guanosine monophosphate(GMP),which is mediated not only by GMP synthase but also by CD39 and CD73,is significantly changed between healthy subjects and patients with COVID-19,as well as between the mild and fatal cases.In addition,dyslipidemia was observed in patients with COVID-19.Overall,the disturbed metabolic patterns have been found to align with the progress and severity of COVID-19.This work provides valuable knowledge about plasma biomarkers associated with COVID-19 and potential therapeutic targets,as well as an important resource for further studies of the pathogenesis of COVID-19. | Di Wu Ting Shu Xiaobo Yang Jian-Xin Song Mingliang Zhang Chengye Yao Wen Liu Muhan Huang Yuan Yu Qingyu Yang Tingju Zhu Jiqian Xu Jingfang Mu Yaxin Wang Hong Wang Tang Tang Yujie Ren Yongran Wu Shu-Hai Lin Yang Qiu Ding-Yu Zhang You Shang Xi Zhou | 2020 | National Science Review2020，7，7： | 9 |
| 4 | SARS-CoV-2-encoded nucleocapsid protein acts as a viral suppressor of RNA interference in cells显示文摘Dear Editor,Coronaviruses (Co Vs) are large enveloped non-segmented positive-strand RNA viruses that broadly distribute among humans and other animal species, including bats, mice and birds. SARS-Co V-2 infections can cause diseases, named the 2019 novel coronavirus disease (COVID-19). The symptoms of COVID-19 range from mild symptoms to severe respiratory syndromes, including pneumonia, and even death(Chen et al., 2020b;Jiang and Shi, 2020;Xia et al., 2020). | Jingfang Mu Jiuyue Xu Leike Zhang Ting Shu Di Wu Muhan Huang Yujie Ren Xufang Li Qing Geng Yi Xu Yang Qiu Xi Zhou | 2020 | Science China(Life Sciences)2020，63，9： | 5 |
| 5 | Volume growth for gradient shrinking solitons of Ricci-harmonic flow显示文摘In this paper,we derive an estimate on the potential functions of complete noncompact gradient shrinking solitons of Ricci-harmonic flow,and show that complete noncompact gradient shrinking Ricci-harmonic solitons have Euclidean volume growth at most. | YANG Fei SHEN JingFang | 2012 | Science China Mathematics2012，55，6： | 5 |
| 6 | Phototransformation of nitrobenzene in the Songhua River:Kinetics and photoproduct analysis显示文摘Nitrobenzene (NB) pollution of the Songhua River caused by an explosion at a petrochemical plant in Jilin City,Jilin Province, China,attracted public concern over the fate of NB in the river.As one of the efforts to predict the fate of residual NB in the river,the kinetics and mechanism of phototransformation of NB in natural water from four sections of the Songhua River were investigated.It was found that photodegradation of NB in water proceeded via pseudo-first-order reaction kinetics under simulated ... | WANG, Aimin HU, Chun QU, Jiuhui YANG, Min LIU, Huijuan RU, Jia QI, Rong SUN, Jingfang | 2008 | Journal of Environmental Sciences2008，20，7： | 4 |
| 7 | Hepatitis C Virus NS2 Protein Suppresses RNA Interference in Cells显示文摘RNAi interference(RNAi)is an evolutionarily conserved post-transcriptional gene silencing mechanism and has been well recognized as an important antiviral immunity in eukaryotes.Numerous viruses have been shown to encode viral suppressors of RNAi(VSRs)to antagonize antiviral RNAi.Hepatitis C virus(HCV)is a medically important human pathogen that causes acute and chronic hepatitis.In this study,we screened all the nonstructural proteins of HCV and found that HCV NS2 could suppress RNAi induced either by small hairpin RNAs(shRNAs)or small interfering RNAs(siRNAs)in mammalian cells.Moreover,we demonstrated that NS2 could suppress RNAi via its direct interaction with doublestranded RNAs(dsRNAs)and siRNAs,and further identified that the cysteine 184 of NS2 is required for the RNAi suppression activity through a serial of point mutation analyses.Together,our findings uncovered that HCV NS2 can act as a VSR in vitro,thereby providing novel insights into the life cycle and virus-host interactions of HCV. | Hui Zhou Qi Qian Ting Shu Jiuyue Xu Jing Kong Jingfang Mu Yang Qiu Xi Zhou | 2020 | Virologica Sinica2020，35，4： | 3 |
| 8 | Spectrofluorimetric analysis of captopril based on its obstruction effect of the nanomaterial surface energy transfer between acridine orange and gold nanoparticles显示文摘A simple and sensitive method for detection of captopril was established based on its obstructive effect on nanomaterial surface energy transfer(NSET). It was found that the acridine orange(AO) could be adsorbed onto the surface of citrated-gold nanoparticles(Au NPs) through electrostatic interaction. Incidentally, the fluorescence of AO was quenched owing to the dipole-dipole interaction of NSET between AO fluorophore and the Au NPs. However, captopril could obstruct the occurrence of NSET between AO and Au NPs effectively with the formation of Au-S covalent bonds between it and the Au NPs. Consequently, AO molecules were moved away from the surface of Au NPs leading to a decline of the energy transfer efficiency. Moreover, the fluorescence of AO could be gradually restored with the addition of captopril. Under the optimal conditions, the recovered fluorescence intensity correlated linearly with the concentration of captopril in the range of 400 nmol/L–2.0 μmol/L with a detection limit of 71 nmol/L. Besides, the proposed method was successfully applied for the detection of captopril in troches with the recovery of 93%–102% and the RSD lower than 2.24%. The results were in good agreement with those obtained from the HPLC method. | Jingfang Guo Yamei Yang Xiaoli Hu Yuanfang Li | 2015 | Science China Chemistry2015，58，5： | 2 |
| 9 | A four-gene signature-derived risk score for glioblastoma:prospects for prognostic and response predictive analyses显示文摘Objective: Glioblastoma(GBM) is the most common primary malignant brain tumor regulated by numerous genes, with poor survival outcomes and unsatisfactory response to therapy.Therefore, a robust, multi-gene signature-derived model is required to predict the prognosis and treatment response in GBM.Methods: Gene expression data of GBM from TCGA and GEO datasets were used to identify differentially expressed genes(DEGs)through DESeq2 or LIMMA methods.The DEGs were then overlapped and used for survival analysis by univariate and multivariate COX regression.Based on the gene signature of multiple survival-associated DEGs, a risk score model was established,and its prognostic and predictive role was estimated through Kaplan–Meier analysis and log-rank test.Gene set enrichment analysis(GSEA) was conducted to explore high-risk score-associated pathways.Western blot was used for protein detection.Results: Four survival-associated DEGs of GBM were identified: OSMR, HOXC10, SCARA3, and SLC39A10.The four-gene signature-derived risk score was higher in GBM than in normal brain tissues.GBM patients with a high-risk score had poor survival outcomes.The high-risk group treated with temozolomide chemotherapy or radiotherapy survived for a shorter duration than the low-risk group.GSEA showed that the high-risk score was enriched with pathways such as vasculature development and cell adhesion.Western blot confirmed that the proteins of these four genes were differentially expressed in GBM cells.Conclusions: The four-gene signature-derived risk score functions well in predicting the prognosis and treatment response in GBM and will be useful for guiding therapeutic strategies for GBM patients. | Mianfu Cao Juan Cai Ye Yuan Yu Shi Hong Wu Qing Liu Yueliang Yao Lu Chen Weiqi Dang XiangZhang Jingfang Xiao Kaidi Yang Zhicheng He Xiaohong Yao Yonghong Cui Xia Zhang Xiuwu Bian | 2019 | Cancer Biology & Medicine2019，16，3： | 2 |
| 10 | Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5′ processing of mitochondrial nad5 messenger RNA and endosperm development显示文摘Pentatricopeptide repeat(PPR) proteins, composing one of the largest protein families in plants,are involved in RNA binding and regulation of organelle RNA metabolism at the posttranscriptional level. Although several PPR proteins have been implicated in endosperm development in rice(Oryza sativa), the molecular functions of many PPRs remain obscure. Here, we identified a rice endosperm mutant named floury endosperm 18(flo18) with pleiotropic defects in both reproductive and vegetative development.Map-based cloning and complementation tests showed that FLO18 encodes a mitochondriontargeted P-type PPR protein with 15 PPR motifs.Mitochondrial function was disrupted in the flo18 mutant, as evidenced by decreased assembly of Complex I in the mitochondrial electron transport chain and altered mitochondrial morphology. Loss of FLO18 function resulted in defective 5′-end processing of mitochondrial nad5 transcripts encoding subunit 5 of nicotinamide adenine dinucleotide hydrogenase. These results suggested that FLO18 is involved in 5′-end processing of nad5 messenger RNA and plays an important role in mitochondrial function and endosperm development. | Mingzhou Yu Mingming Wu Yulong Ren Yihua Wang Jingfang Li Cailin Lei Yinglun Sun Xiuhao Bao Hongming Wu Hang Yang Tian Pan Yongfei Wang Ruonan Jing Mengyuan Yan Houda Zhang Lei Zhao Zhichao Zhao Xin Zhang Xiuping Guo Zhijun Cheng Bing Yang Ling Jiang Jianmin Wan | 2021 | Journal of Integrative Plant Biology2021，63，5： | 2 |
| 11 | From mandatory icebreaker guiding to a permission regime: changes to the new Russian legislation of the Northern Sea Route显示文摘This article focuses on two issues. The first concerns definitions of the Northern Sea Route(NSR) in old and new Russian legislation, and the second relates to Russian rules on icebreaker guiding. Based on a comprehensive comparative analysis of relevant Russian legal provisions enacted in 2013 and previous laws in this area, we offer the following conclusions.(1) Our legal analysis indicates that Russia's view of the NSR as a historical national transportation route has not changed. However, the new law redefines the scope and coverage of the NSR, which now comprises the internal waters, territorial sea, adjacent zone, and the exclusive economic zone of the Russian Federation. In fact, the new law resolves previous ambiguity regarding extension of the NSR boundary to the high seas.(2) Based on an analysis of the new rules on icebreaker guiding, the article concludes that NSR is transitioning from a mandatory icebreaker guiding regime into a permit regime. This is particularly evident in its provision of a concrete, practical, and predictable clause on permissible or impermissible conditions relating to independent navigation. According to the new rules, it is possible for foreign ships to undertake independent navigation in the NSR. The Russian NSR policy, therefore, appears to have changed significantly, and has future potential for opening the NSR up to the international community. | ZHANG Xia ZOU Leilei TU Jingfang QIAN Zongqi WANG Zeming YANG Huigen | 2014 | Advances in Polar Science2014，25，3： | 2 |
| 12 | Downregulation of WIF-1 by hypermethylation in astrocytomas显示文摘当一个 Wnt 对手和肿瘤 suppressor,而是 WIF-1 基因倡导者和 WIF-1 的低表示的 hypermethylation 激活异常地发信号的 Wnt 并且导致几个人的肿瘤的开发, Wnt 禁止的 factor-1 (WIF-1 ) 行动。由使用 RT-PCR, immunohistochemistry 和 methylation 特定的 PCR,我们在 4 正常大脑纸巾分析了 WIF-1 的表示和 methylation, 35 刚 resected 星细胞瘤纸巾和 4 根导出 glioblastoma 的房间线。WIF-1 mRNA 和蛋白质表示层次的重要 downregulation 与正常大脑纸巾相比在星细胞瘤纸巾被观察。在 WIF-1 downregulation 和星细胞瘤的病理学的等级之间的重要协会被发现。WIF-1 基因异常 methylation 在 35 中的 19 个被观察(54.29%) 肿瘤样品。倡导者 methylation 肿瘤显示出低 WIF-1 蛋白质和 mRNA 表示,而倡导者 unmethylation 肿瘤显示了高蛋白质和 mRNA 表示层次。而且,, WIF-1 mRNA 表示的完全的缺席在四根房间线被观察有 demethylating 代理人的处理, 5-aza-2-deoxycytidine,恢复 WIF-1 表示。我们的结果建议 WIF-1 基因经常是在由异常倡导者 methylation 的星细胞瘤的 silenced。这可以是在星细胞瘤 carcinogenesis 的重要机制。 | Zhuanyi Yang Ying Wang Jiasheng Fang Fenghua Chen Jingfang Liu Jun Wu Yanjin Wang Tao Song Feiyue Zeng Yihua Rao | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 2 |
| 13 | OsbZIP72 Is Involved in Transcriptional Gene-Regulation Pathway of Abscisic Acid Signal Transduction by Activating Rice High-Affinity Potassium Transporter OsHKT1;1显示文摘We created CRISPR-Cas9 knock-out and overexpressing OsbZIP72 transgenic rice plants to gain a better understanding of the role and molecular mechanism of OsbZIP72 gene in stress tolerance,which has remained largely elusive.OsbZIP72 was expressed and integrated into rice transgenic plant genomes,and the OsbZIP72 transcript in overexpression lines was elicited by salinity,abscisic acid(ABA)and drought stresses.OsbZIP72 overexpressing plants showed higher tolerance to drought and salinity stresses,while knock-out transgenic lines showed higher sensitivity to these stresses.The differentially expressed genes(DEGs)from RNA-sequencing data encompassed several abiotic stress genes,and the functional classification of these DEGs demonstrated the robust transcriptome diversity in OsbZIP72.Yeast one-hybrid,along with luciferase assay,indicated that OsbZIP72 acted as a transcriptional initiator.Remarkably,electrophoresis mobility assay revealed that OsbZIP72 bound directly to the ABAresponsive element in the OsHKT1;1 promoter region and activated its transcription.Overall,our findings revealed that OsbZIP72 can act as a transcriptional modulator with the ability to induce the expression of OsHKT1;1 in response to environmental stress through an ABA-dependent regulatory pathway,indicating that OsbZIP72 can play a crucial role in the ABA-mediated salt and drought tolerance pathway in rice. | WANG Baoxiang LIU Yan WANG Yifeng LI Jingfang SUN Zhiguang CHI Ming XING Yungao XU Bo YANG Bo LI Jian LIU Jinbo CHEN Tingmu FANG Zhaowei LU Baiguan XU Dayong Babatunde Kazeem BELLO | 2021 | Rice science2021，28，3： | 2 |
| 14 | Study on the structure and tribological properties of surface-modified Cu nanoparticles显示文摘 | Zhou Jingfang Yang Jianjun Zhang Zhijun | 1999 | Materials Research Bulletin1999，34，9： | 1 |
| 15 | Study on the structure and tribological properties of surface-modified Cu nanoparticles显示文摘 | Zhou Jingfang Yang Jianjun Zhang Zhijun | 1999 | Mater Res Bull1999，34，9： | 1 |
| 16 | Study on the Structure and Tribological Properties of Surface - modified Cu Nanoparticles显示文摘 | Jingfang Zhou Jianjun Yang Zhijun Zhang | 1999 | Materials Research Bulletin1999，34，9： | 1 |
| 17 | , Multiple replication origins with diverse control mechanisms in Haloarcula hispanica 显示文摘 | Zhenfang Wu Jingfang Liu Haibo Yang | 2013 | Nucleic Acids Research2013，113，： | 1 |
| 18 | Study on the structure and tribological properties of urface-modified Cu nsnoparticles显示文摘 | ZHOU Jingfang YANG Jianjun ZHANG Zhijun | 1999 | Materials Research Bulletin1999，34，9： | 1 |
| 19 | 显示文摘 | Zhou Jingfang Yang Jianjun Zhang Zhijun | 1999 | Wear1999，34，9： | 1 |
| 20 | Effects of Plant Spacing,Row Spacing and Seedling Number Allocation on the Growth Dynamics of Different Populations of Two Types of Rice Varieties显示文摘[Objectives]This study was conducted to construct high-yielding,high-quality,and high-light-efficiency population structures of different types of rice varieties.[Methods]The effects of plant spacing,row spacing and seedling number allocation on stems and tillers,leaf area and dynamic traits of high-yielding rice populations(more than 9750 kg/hm^(2))and low-yielding populations(less than 9750 kg/hm^(2))were studied by two kinds of representative rice varieties with different tiller ability and panicle sizes in cold regions.[Results]Decreasing the plant and row spacing and increasing the number of planted seedlings per hole advanced the heading date and made the number of stems and tillers in the population increase.The impact on the super rice variety with fewer tillers and heavier panicles was greater than that of conventional variety with more tillers and lighter panicles.The stems and tillers of the high-yielding high-quality populations grew steadily in the early stage of development,until an appropriate number of panicles was reached at the critical leaf age for productive tillers(June 25),and the peak seedlings should appear in the jointing stage(July 9)with a small number(that value of Longjing 21,the super rice variety with fewer tillers and heavier panicles,was about 1.2 times the expected panicle number,and that of the conventional variety with more tillers and lighter panicles,Kongyu 131,was about 1.1 times the expected panicle number).The populations gradually declined since then,until reaching an appropriate number of panicles at the heading stage.The high-yielding high-quality populations had a higher leaf area index at the heading stage.The value of Longjing 21,the super rice variety with fewer tiller and heavier panicles,reached about 6.0,and that of Kongyu 131,the conventional variety with more tillers and lighter panicles,reached about 4.5.The values decreased thereafter.At the maturation stage,the leaf area index of Longjing 21 remained above 2.5,and that of Kongyu 131 remained above 2.0.[Conclusions]This study has an important guiding role and significance for their high-yielding and high-quality supporting cultivation in cold regions. | Jingfang XUE Guangshan ZHAO Shuqiang CHEN Xiaodong DU Limin YANG Haixin ZHAO Yongsheng CAI Tong ZHOU Linan WANG Mingxu ZHOU | 2021 | Agricultural Biotechnology2021，10，2： | 1 |
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