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| 1 | 利用β-环糊精键合固定相分离纯化葛根素显示文摘使用β 环糊精(β CD)与琼脂糖凝胶偶联合成的几种新型键合固定相对葛根黄酮进行液相色谱分离纯化,分离效果最好的介质为聚β CD偶联烯丙基 SepharoseHP所得的固定相,该固定相湿凝胶的样品负载量达1 21g/L。色谱分离时采用的流动相为10%(体积分数)醋酸水溶液,葛根素的收率在97%以上时,其纯度高于93%。 | 贺湘凌 谭天伟 JANSON Jan-Christer | 2003 | 色谱2003，21，6： | 22 |
| 2 | 长白山白眉蝮蛇毒类凝血酶Gussurobin基因的克隆、表达与纯化(英文)显示文摘根据同源性 ,在高度保守的上游信号肽区域设计引物 ,通过RT PCR反应 ,从长白山白眉蝮蛇 (Gloydiusussurensis)毒腺总RNA中克隆得到类凝血酶 gussurobincDNA ,双向测序得到 gussurobin基因的全序列并由此推测出相应的氨基酸序列。与其他已知的类凝血酶不同 ,gussurobin只含有一个可能的糖基化位点 ,即Asn12 4 Ser12 5 Thr12 6。将gussurobin基因克隆到表达载体 pPIC9K中 ,电极转化至毕氏酵母菌株GS115中 ,经G418抗性筛选和营养缺陷型筛选获得重组子。经摇瓶培养 ,获得表达。经过柱层析分离 ,获得SDS PAGE电泳纯的重组gussurobin。 | 杨青 胡学军 许小明 安利佳 袁晓东 苏志国 JANSON Jan-Christer | 2002 | 生物化学与生物物理学报2002，34，1： | 10 |
| 3 | Digitized Outcrop Geomodeling of Ramp Shoals and its Reservoirs: as an Example of Lower Triassic Feixianguan Formation of Eastern Sichuan Basin显示文摘Numerous hydrocarbon accumulations are found in ramp crest shoals worldwide and therefore this depositional setting has a high potential of being the hydrocarbon reservoir.In this paper,we combined digital outcrop geology and traditional geological mapping to build an outcrop-based geocellular model of the ramp-crest shoal complex of the Lower Triassic Feixianguan Formation in the Eastern Sichuan Basin.The outcrop model serves as an analogue for the subsurface reservoir of the Feixianguan Formation and illustrates the complexity of the lithofacies types,stratigraphic architecture,and reservoir heterogeneities at a scale below conventional subsurface data resolution.The studied ramp-crest shoal complex consists of thirteen types of lithofacies that can be grouped into three facies-groups corresponding to subtidal intraclastic shoal,sub-to inter-tidal oolitic shoal,and tidal flat depositional environments respectively.The stratigraphic architecture of the shoal complex shows mostly a strong progradation of the high energy facies associated with an overall decrease of accommodation space associated with relative sea level still stand.Two reservoir facies associations have been recognized.The first one consists of supratidal dolomudstone and upper intertidal partially dolomitized oolitic packstone with anhydrite or nodules.These facies were deposited above the high energy oolitic grainstones and occurs as thin-bedded and laterally continuous layers,characterized by high porosity and low permeability.The second reservoir facies association is composed of intertidal crystalline dolomite and subtidal intraclastic bindstone that occurs stratigraphically below the oolitic grainstones.These deposits consist of massive laterally discontinuously beds,and are characterized by high porosity and high permeability.Both types of reservoir facies tend to be stacked vertically and migrated laterally with the progradation of the shoal complex.The construction of the outcrop-based 3D geological model provide a description and quantification of the facies distribution within a robust stratigraphic framework and the style and amount of reservoir heterogeneities associated with a ramp-crest shoal complex reservoir such as the one found in Lower Triassic Feixianguan Formation and Cambrian Longwangmiao Formation in Sichuan Basin or other ramp-crest reservoir worldwide. | QIAO Zhanfeng SHEN Anjiang ZHENG Jianfeng Xavier JANSON CHANG Shaoying WANG Xiaofang CHEN Yana LIAO Yisha | 2017 | Acta Geologica Sinica(English Edition)2017，91，4： | 8 |
| 4 | Improvement of quantitative testing of liver function in patients with chronic hepatitis C after installment of antiviral therapy显示文摘AIM: To investigate if and to what extent antiviral therapy influenced a broad panel of quantitative testing of liver function (QTLF).METHODS: Fifty patients with chronic hepatitis C were either treated with interferon (n = 8), interferon/ribavirin (n = 19) or peg-interferon/ribavirin (n = 23). Quantitative testing of liver function, including aminopyrine breath test (ABT), galactose elimination capacity (GEC), sorbitol clearance (SCI) and indocyanine green clearance (ICG)was performed before and 3 mo after initiation of antiviral therapy.RESULTS: After 3 mo of antiviral treatment, 36 patients showed normal transaminases and were negative for HCV-RNA, 14 patients did not respond to therapy. ABT and GEC as parameters of microsomal and cytosolic liver function were reduced in all patients before therapy initiation and returned to normal values in the 36 therapy responders after 3 mo. Parameters of liver perfusion (SCI and ICG) were not affected by antiviral therapy. In the 14 non-responders,no changes in QTLF values were observed during the treatment period.CONCLUSION: ICG and SCI remained unaffected in patients with chronic hepatitis C, while ABT and GEC were significantly compromised. ABT and GEC normalized in responders to antiviral therapy. Early determination of ABT and GEC may differentiate responders from non-responders to antivJral treatment in hepatitis C. | Matthias Ocker Marion Ganslmayer Steffen Zopf Susanne Gahr Christopher Janson Eckhart G. Hahn Christoph Herold | 2005 | World Journal of Gastroenterology2005，11，35： | 8 |
| 5 | β-环糊精键合固定相分离纯化银杏叶提取物和银杏黄酮显示文摘目的 考察以β-环糊精(β-CD)为键合固定相的色谱柱分离纯化银杏叶提取物和银杏黄酮的影响因素。方法 以色谱图中色谱峰的个数和目标物为离度为指标,采用低压液相色谱技术对银杏叶提取物和银杏黄酮进行分离纯化。同时比较固定相、流动相、缓冲液、pH值、洗脱速度和上样体积对分离结果的影响。结果 氯代环氧丙烷偶联β-CD-Superose 12 pg键合相能够较好地分离银杏叶提取物和银杏黄酮,优于葡聚糖凝胶Sephardex LH-20。结论 β-环糊精键合固定相适用于银杏叶提取物和银杏黄酮的分离与纯化。 | 邓利 孙猛 谭天伟 王芳 Janson Jan Christer | 2004 | 中草药2004，35，10： | 7 |
| 6 | 蛋白质在层析过程中的失活与复性显示文摘在层析分离过程中 ,层析的固相介质和溶液环境会引起蛋白质的结构变化 :天然的蛋白质可能会降低活性甚至完全失去活性 ,而结构变化了的蛋白质也可能重新折叠恢复活性 .此类现象已经引起研究者特别是生化工程学家的关注 ,层析介质的种类和操作手段是影响此两个过程的关键因素 .利用不同的层析方法 ,对不同的蛋白质采取相应的措施有利于提高活性蛋白质的回收率 ,具有重要的理论和应用价值 . | 李明 Jan-Christer Janson 苏志国 | 2004 | 化工学报2004，55，7： | 5 |
| 7 | 混合模式吸附色谱分离纯化贻贝粘蛋白的研究显示文摘[目的]研究高强度琼脂糖基质混合模式吸附介质分离纯化贻贝粘蛋白的方法并探讨分离机理。[方法]考察在不同酸碱度、不同离子强度下,贻贝粘蛋白在凝胶上的静态吸附、动态吸附及解吸附过程。[结果]氯化钠浓度从0增加到0.5mol/L,介质和蛋白之间平衡吸附量变化在6%以内;而酸碱度对吸附的影响较大,当酸碱度跨越蛋白等电点时,单位酸碱度(pH)最大吸附量变化百分比达23%。[结论]介质的复合配基与贻贝粘蛋白间表现了离子交换、疏水作用、氢键吸附的混合吸附作用,采用酸碱度高于等电点的洗脱液洗脱贻贝粘蛋白,得到纯度为90%的贻贝粘蛋白。 | 范恒瑞 高敏 Jan-Christer Janson 顾铭 | 2010 | 安徽农业科学2010，38，28： | 4 |
| 8 | 表面活性剂辅助重组蛋白质复性显示文摘以重组人溶菌酶(rhLys)与重组β甘露聚糖酶(rMan)为体系,综合采用活性测定、非还原性SDSPAGE以及荧光发射光谱分析等方法研究了十六烷基三甲基溴化铵(CTAB)、CTAB与β环糊精(βCD)组成的人工分子伴侣以及其他种类复性助剂对重组蛋白质复性过程的影响.结果表明CTAB及人工分子伴侣均可有效地辅助rhLys复性,且rhLys与鸡卵清溶菌酶(Lys)呈现出类似的复性过程特性;人工分子伴侣可显著地提高rMan在高浓度下的复性率;表面活性剂带电性质、表面活性剂与蛋白质的摩尔比以及变性蛋白质的浓度等是影响复性率及复性产品分布的主要因素.这些结果对于此类复性技术应用于重组蛋白体系时的工艺选择和优化提供了重要的依据和参考. | 王君 杨君秋 刘铮 Jan-Christer Janson | 2005 | 化工学报2005，56，7： | 3 |
| 9 | 疏水相互作用与亲水相互作用组合色谱纯化缩宫素显示文摘将疏水相互作用色谱与亲水相互作用色谱结合,建立了用于化学合成缩宫素粗品分离纯化的两步纯化工艺.先以纯水为流动相,采用Nylon6疏水相互作用色谱对粗品进行脱盐与富集,后以Superose12亲水相互作用色谱进行进一步纯化,两步纯化后,峰面积纯度达93.5%,提高了5.96倍,总回收率为50.1%,与用C18为介质的传统两步反相色谱纯化的结果相当.本方法有机溶剂用量仅为传统反相色谱的1/7,且两种介质都能耐受极端pH值清洗条件,重复利用率高. | 张晓鸥 宋茂谦 王长海 Jan-Christer Janson 顾铭 | 2010 | 过程工程学报2010，10，3： | 2 |
| 10 | 预防性植入合成补片在腹腔镜造口术中的应用显示文摘目的探究腹腔镜造口术中预防性应用补片的效果。方法将腹腔镜乙状结肠造口术中预防性地在腹膜外植入大孔径、轻量型补片的病例纳入研究。术后随访至少12个月。结果 2003-2007年共有25例病人接受了乙状结肠造口术。平均年龄为65(31~89)岁,BMI平均为26(21~32),其中15例为女性。术后1例病人发生造口坏死,2例发生轻度切口感染。在所有接受随访的20例中(平均随访时间19个月,11~31个月),有3例(15%)发生了造口旁疝。无术后瘘管形成和造口狭窄,也未发生补片感染和再次手术移除补片的情况。结论在腹腔镜造口术中预防性地在腹膜外植入大网孔、轻量型补片是简单安全的操作,并且能有效降低术后造口旁疝的发生率。 | Janson AR Janes A Israelsson LA | 2011 | 中国实用外科杂志2011，31，8： | 2 |
| 11 | Acoustic Radiation Force Impulse (ARFI) zur Messung der Leberelastizit?t: Eine alternative nicht invasive Methode zur Bestimmung des Leberfibrosegrades bei viraler Hepatitis显示文摘 | R. Goertz Y. Zopf V. Jugl R. Heide C. Janson D. Strobel T. Bernatik T. Haendl | 2010 | Ultraschall in Med2010，，02： | 2 |
| 12 | The Development of Gel Media and Columns for Large-Scale Chromatography of Proteins, a Historical Review显示文摘The first dedicated protein chromatography media were introduced during the 1950s and 1960s. There was an early awareness of the possibility of using these for production applications within the biopharmaceutical industry. However, the crucial limitation was the fact that those media that were most compatible with proteins lent themselves less favourably to scaling-up. The problems were primarily physical. Thus the fibrous cellulose media showed bed cracking tendencies and the bead shaped polyacrylamide, dextran, and agarose gel media, then available, were too soft to stand the hydrodynamic forces acting in large columns, leading to bed compaction and increased pressure drop. At the time, the best solution to the latter problem, after a number of intermediary solutions were tried, was the introduction of the stacked column concept in which several short column segments were connected by small bore tubing, thus reducing the force acting on the particles in each bed copartment. However, the ultimate remedy, the introduction of chromatographic matrices that combine the desired features of adequate rigidity, macroporosity, biocompatibility, chemical stability (for CIP and SIP) and derivatizability, did not occur until the middle of the 1980s when adquately cross-linked agarose gel media such as Sepharose Fast Flow were made available. The paper also recognizes the many attempts made during the past 50 years to develop continous chromatography columns. Most of the designs are based on an annular bed or on an array of anularly arranged parallel columns continuously fed with samples in a cyclic manner. The introduction of media and columns for expanded bed adsorption followed a demand for fewer purification steps and shorter process times. In recent years, columns have been introduced that allow packing and repacking without needing to open the column. The review provides an historical account of the developments that have led to the present state-of-the-art both regarding large diameter columns and gel media intended for industrial applications of protein chromatography and also discusses the current trends that point to possible future applications. | Jan-Christer Janson | 2002 | Chinese Journal of Chemical Engineering2002，10，6： | 2 |
| 13 | What characterizes patients who are unable to tolerate continuous positive airway pressure (CPAP) treatment 显示文摘 | Janson C Noges E Svedberg2Randt S | 2000 | Respir Med2000，94，： | 1 |
| 14 | Fertility and pregnancy outcome in women with congenital adrenal hyperplasiadue to 21-hydroxylase deficiency 显示文摘 | Hagenfeldt K Janson P O Holmdahl G | 2008 | Human Reproduction2008，23，7： | 1 |
| 15 | Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes 显示文摘 | Butler AE Janson J Bonner-Weir S | 2003 | Diabetes2003，52，1： | 1 |
| 16 | What characterizes patients who are unable to tolerate continuous positive airway pressure(CPAP)treatment?显示文摘 | Janson C Noges E Svedberg-Randt S et a1 | 2000 | Respir Med2000，94，： | 1 |
| 17 | Three distinct modes in a cold atmospheric pressure plasma jet显示文摘 | Walsh J L Iza F Janson N B | 2010 | Journal of Physics D: Applied Physics2010，43，7： | 1 |
| 18 | Dividends policy and ownership structure in C hina显示文摘 | Gan Wei Weiguo Zhang Janson Ze Zhong Xiao | 2003 | Rochester:SSRN Working Paper Series2003，2003，： | 1 |
| 19 | Gonadotropin-and cytokine-regulated expression of the chemokine interleukin-8 in the human preovulatory follicle of the menstrual cycle 显示文摘 | Runesson E Ivarsson K Janson P O | 2000 | J Clin Endocrinol Metab2000，85，11： | 1 |
| 20 | Characterisation of rpsL,rrs and embB mutations associated with streptomycin and ethambutol resistance in Mycobacterium tuberculosis显示文摘 | Tracevska T Jansone I Nodieva A | 2004 | Res Microbiol2004，155，10： | 1 |
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