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| 1 | Development of general practice education and training in China显示文摘General practice as a discipline has largely evolved over the past 30 years in the field of primary health care.It originated in the 18th century in Europe.It was established in the United States in the 1960s and introduced to China in the late 1980s.In earlier times,medicine was not specialized,and general physicians dominated the medical field.1 At the end of the 19th century,with the development of basic medical disciplines,such as biology and anatomy,the development of more specialized medical practices began.Medical specialists increased in number while general physicians declined.By the late 1980s,2 an aging population,increased urbanization,increased incidence of infectious diseases,and rising healthcare costs made the practice of family medicine more common.3 Preparation for the establishment of the academic discipline of family medicine began officially in 1989 with the foundation of the General Practice Training Center (GPTC) in Beijing.In 1999,family medicine achieved formal status as an academic discipline. | Ren Wen Liu Ying Qiu Yan Ren Jingjing | 2014 | Chinese Medical Journal2014，，17： | 10 |
| 2 | Defined tumor antigen-specific T cells potentiate personalized TCR-T cell therapy and prediction of immunotherapy response显示文摘Personalized immunotherapy targeting tumor-specific antigens(TSAs)could generate efficient and safe antitumor immune response without damaging normal tissues.Although neoantigen vaccines have shown therapeutic effect in clinic trials,precise prediction of neoantigens from tumor mutations is still challenging.The host antitumor immune response selects and activates T cells recognizing tumor antigens.Hence,T cells engineered with T-cell receptors(TCRs)from these naturally occurring tumor antigen-specific T(Tas)cells in a patient will target personal TSAs in his/her tumor.To establish such a personalized TCR-T cell therapy,we comprehensively characterized T cells in tumor and its adjacent tissues by single-cell mRNA sequencing(scRNA-seq),TCR sequencing(TCR-seq)and in vitro neoantigen stimulation.Compared to bystander T cells circulating among tissues,Tas cells were characterized by tumor enrichment,tumor-specific clonal expansion and neoantigen specificity.We found that CXCL13 is a unique marker for both CD4^(+)and CD8^(+)Tas cells.Importantly,TCR-T cells expressing TCRs from Tas cells showed significant therapeutic effects on autologous patient-derived xenograft(PDX)tumors.Intratumoral Tas cell levels measured by CXCL13 expression precisely predicted the response to immune checkpoint blockade,indicating a critical role of Tas cells in the antitumor immunity.We further identified CD200 and ENTPD1 as surface markers for CD4^(^(+))and CD8^(^(+))Tas cells respectively,which enabled the isolation of Tas cells from tumor by Fluorescence Activating Cell Sorter(FACS)sorting.Overall,our results suggest that TCR-T cells engineered with Tas TCRs are a promising agent for personalized immunotherapy,and intratumoral Tas cell levels determine the response to immunotherapy. | Jingjing He Xinxin Xiong Han Yang Dandan Li Xuefei Liu Shuo Li Shuangye Liao Siyu Chen Xizhi Wen Kuai Yu Lingyi Fu Xingjun Dong Kaiyu Zhu Xiaojun Xia Tiebang Kang Chaochao Bian Xiang Li Haiping Liu Peirong Ding Xiaoshi Zhang Zhenjiang Liu Wende Li Zhixiang Zuo Penghui Zhou | 2022 | Cell Research2022，32，6： | 10 |
| 3 | Recent advances in mammalian reproductive biology显示文摘Reproductive biology is a uniquely important topic since it is about germ cells, which are central for transmitting genetic information from generation to generation. In this review, we discuss recent advances in mammalian germ cell development,including preimplantation development, fetal germ cell development and postnatal development of oocytes and sperm. We also discuss the etiologies of female and male infertility and describe the emerging technologies for studying reproductive biology such as gene editing and single-cell technologies. | Lu Wen Qiang Liu Jingjing Xu Xixi Liu Chaoyi Shi Zuwei Yang Yili Zhang Hong Xu Jiang Liu Hui Yang Hefeng Huang Jie Qiao Fuchou Tang Zi-Jiang Chen | 2020 | Science China(Life Sciences)2020，63，1： | 6 |
| 4 | Fasudil hydrochloride differentiates bone marrow mesenchymal stem cells into neurons via notch signaling显示文摘BACKGROUND: Notch signaling regulates bone marrow mesenchymal stem cell (MSC) proliferation, differentiation, and apoptosis, Notch signaling and Rho kinase signaling exhibit a crosstalk phenomenon with JAK/STAT, and both participate in the neuronal dendritic spine development. Inhibition of RhoA/Rho kinase signaling may regulate MSC differentiation into neuronal-like cells. OBJECTIVE: To investigate the effect of Notch1 signaling on the differentiation of rat MSCs into neurons induced by fasudil hydrochloride (C14H17N3O2S·HCl), a Rho kinase inhibitor, through a siRNA approach. DESIGN, TIME AND SETTING: An in vitro cytological experiment was performed in the Cell Laboratory of Henan Academy of Medical and Pharmaceutical Sciences between December 2007 and May 2009. MATERIALS: MSCs were obtained from Wistar rat femoral bone, fasudil hydrochloride was provided by Tianjin Chase Sun Pharmaceutical Co., Ltd. Rn-notch1-siRNa, negative control siRNA (Cy3 label) and Rn-MAPK1 control siRNA were provided by QIAGEN, Coloqne, German. METHODS: The cultured MSCs were divided into non-transfected, transfected group (transfected with Rn-Notch1-siRNA), positive control (transfected with Rn-MAPK-1 control siRNA), and negative control (transfected with negative control siRNA) groups. Fasudil hydrochloride was applied to induce MSCs to differentiate into neurons. MAIN OUTCOME MEASURES: The fluorescence expression by the transfected MSCs was observed under an inverted fluorescence microscope; the expression of Notch1 mRNA, Hes1 mRNA, and MAPK1 mRNA in MSCs was detected by reverse transcription polymerase chain reaction; the expression of Notch1 protein, nestin, neurofilament M, and glial fibrillary acidic protein was detected by immunocytochemistry. The viability of MSCs was detected by tetrazolium bromide assay. RESULTS: MSC fluorescence increased following a 72-hour siRNA transfection, with transfection efficiencies of up to (0.91 ± 0.04); the Notch1 mRNA and Hes1 mRNA expressed by transfected MSCs was significantly decreased (P < 0.05) compared with non-transfected cells. Fasudil hydrochloride induced MSCs to differentiate into neurons with greater efficiency in the transfected group (P < 0.05). CONCLUSION: Fasudil hydrochloride induces rat MSCs to differentiate into neurons; inhibition of Notch1 signaling and Hes1 expression may jointly promote the differentiation of MSCs into neurons. | Eryi Zhao Liudong Wang Quanqing Wen Wenjuan Guan Jingjing Lu Tao Peng Boai Zhang Yanjie Jia | 2010 | Neural Regeneration Research2010，5，11： | 5 |
| 5 | Multi-scale validation strategy for satellite albedo products and its uncertainty analysis显示文摘Coarse-resolution satellite albedo products are important for climate change and energy balance research because of their capability to characterize the spatiotemporal patterns of land surface parameters at both the regional and global scales. The accuracy of coarse-resolution products is usually assessed via comparison with in situ measurements. The key issue in the comparison of remote sensing observations with in situ measurements is scaling and uncertainty. This paper presents a strategy for validating 1-km-resolution remote sensing albedo products using field measurements and high-resolution remote sensing observations. Field measurements were collected to calibrate the high-resolution(30 m) albedo products derived from HJ-1a/b images. Then, the calibrated high-resolution albedo maps were resampled(i.e., upscaled) to assess the accuracy of the coarse-resolution albedo products. The samples of field measurements and high-resolution pixels are based on an uncertainty analysis. Two types of coarse-resolution albedo datasets, from global land surface satellite(GLASS) and moderate-resolution imaging spectroradiometer(MODIS), are validated over the middle reaches of the Heihe River in China. The results indicate that the upscaled HJ(Huan Jing means environment in Chinese and this refers to a satellite constellation designed for environment and disaster monitoring by China) albedo, which was calibrated using field measurements, can provide accurate reference values for validating coarse-resolution satellite albedo products. However, the uncertainties in the upscaled HJ albedo should be estimated, and pixels with large uncertainties should be excluded from the validation process. | PENG JingJing LIU Qiang WEN JianGuang LIU QinHuo TANG Yong WANG LiZhao DOU BaoCheng YOU DongQin SUN ChangKui ZHAO XiaoJie FENG YouBin SHI Jian | 2015 | Science China Earth Sciences2015，58，4： | 5 |
| 6 | Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs显示文摘Heat stress(HS)can be detrimental to the gut health of swine.Many negative outcomes induced by HS are increasingly recognized as including modulation of intestinal microbiota.In turn,the intestinal microbiota is a unique ecosystem playing a critical role in mediating the host stress response.Therefore,we aimed to characterize gut microbiota of pigs’exposure to short-term HS,to explore a possible link between the intestinal microbiota and HS-related changes,including serum cytokines,oxidation status,and intestinal epithelial barrier function.Our findings showed that HS led to intestinal morphological and integrity changes(villus height,serum diamine oxidase[DAO],serum D-lactate and the relative expressions of tight junction proteins),reduction of serum cytokines(interleukin[IL]-8,IL-12,interferongamma[IFN-g]),and antioxidant activity(higher glutathione[GSH]and malondialdehyde[MDA]content,and lower superoxide dismutase[SOD]).Also,16S rRNA sequencing analysis revealed that although there was no difference in microbial a-diversity,some HS-associated composition differences were revealed in the ileum and cecum,which partly led to an imbalance in the production of short-chain fatty acids including propionate acid and valerate acid.Relevance networks revealed that HS-derived changes in bacterial genera and microbial metabolites,such as Chlamydia,Lactobacillus,Succinivibrio,Bifidobacterium,Lachnoclostridium,and propionic acid,were correlated with oxidative stress,intestinal barrier dysfunction,and inflammation in pigs.Collectively,our observations suggest that intestinal damage induced by HS is probably partly related to the gut microbiota dysbiosis,though the underlying mechanism remains to be fully elucidated. | Bing Xia Weida Wu Wei Fang Xiaobin Wen Jingjing Xie Hongfu Zhang | 2022 | Animal Nutrition2022，，1： | 3 |
| 7 | Influence of Ni interlayer on interfacial microstructure and mechanical properties of Ti-6Al-4V/AZ91D bimetals fabricated by a solid–liquid compound casting process显示文摘The solid–liquid compound casting of Mg-AZ91D and Ti-TC4 alloys was developed by using pure Ni electro-deposited coating.The pouring temperatures of 660℃,690℃,720℃and 750℃were chosen to investigated the effects of casting temperatures on microstructural evolution,properties,and fracture behaviors of Ni-coated TC4/AZ91D bimetals by the solid–liquid compound casting(SLCC).The scanning electron microscopy(SEM)and the energy dispersive spectroscopy(EDS)results showed that the interfacial zone mainly composed of nickel,Mg_(2)Ni and Mg-Al-Ni in the bimetals cast at 660℃.As the pouring temperature was increased to 750℃,the width of the interface zone,which mainly composed ofδ(Mg),Mg_(2)Ni,Mg-Al-Ni,Mg_(3)TiNi_(2) and Al_(3)Ni,gradually increased.The microhardness tests showed that the micro-hardness of the interface zone was smaller than that of TC4 substrate but larger than that of the cast AZ91D matrix.At the pouring temperature of 720℃,the Ni-coated TC4/AZ91D bimetals had the most typical homogeneous interface,which had granular Mg-Al-Ni ternary phase but no ribbon-like Al3Ni binary phase,and achieved the highest shear strength of 97.35MPa.Meanwhile,further fracture behavior analysis showed that most fracture failure of Ni-coated TC4/AZ91D bimetals occurred at the Mg_(2)Ni+δ(Mg)eutectic structure and Al_(3)Ni hard intermetallic. | Fulin Wen Jianhua Zhao Miaowang Yuan Jingfeng Wang Dengzhi Zheng Jinyong Zhang Ke He Jingjing Shangguan Yu Guo | 2021 | Journal of Magnesium and Alloys2021，9，4： | 3 |
| 8 | DNA damage repair promotion in colonic epithelial cells by andrographolide downregulated cGAS-STING pathway activation and contributed to the relief of CPT-11-induced intestinal mucositis显示文摘Gastrointestinal mucositis is one of the most debilitating side effects of the chemotherapeutic agent irinotecan(CPT-11). Andrographolide, a natural bicyclic diterpenoid lactone, has been reported to possess anti-colitis activity. In this study, andrographolide treatment was found to significantly relieve CPT-11-induced colitis in tumor-bearing mice without decreasing the tumor suppression effect of CPT-11. CPT-11 causes DNA damage and the release of double-stranded DNA(ds DNA) from the intestine, leading to cyclic-GMP-AMP synthase(c GAS)-stimulator of interferon genes(STING)-mediated colitis, which was significantly decreased by andrographolide both in vivo and in vitro. Mechanistic studies revealed that andrographolide could promote homologous recombination(HR) repair and downregulate ds DNA-c GAS-STING signaling and contribute to the improvement of CPT-11-induced gastrointestinal mucositis. These results suggest that andrographolide may be a novel agent to relieve gastrointestinal mucositis caused by CPT-11. | Yuanyuan Wang Bin Wei Danping Wang Jingjing Wu Jianhua Gao Haiqing Zhong Yang Sun Qiang Xu Wen Liu Yanhong Gu Wenjie Guo | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 3 |
| 9 | A core-shell structured COYID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity显示文摘Although inoculation of COVID-19 vaccines has rolled out globally,there is still a critical need for safe and effective vaccines to ensure fair and equitable supply for all countries.Here,we report on the development of a highly efficacious mRNA vaccine,SW0123 that is composed of sequence-modified mRNA encoding the full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex(LPP)nanoparticles.SWOT 23 is easy to produce using a large-scale microfluidics-based apparatus.The unique core-shell structured nanoparticle facilitates vaccine uptake and demonstrates a high colloidal stability,and a desirable biodistribution pattern with low liver targeting effect upon intramuscular administration.Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123,represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2,but also a panel of variants including D614G and N501Y variants.In addition,SW0123 conferred effective protection in both mice and non-human primates upon SARS-CoV-2 challenge.Taken together,SW0123 is a promising vaccine candidate that holds prospects for further evaluation in humans. | Ren Yang Yao Deng Baoying Huang Lei Huang Ang Lin Yuhua Li Wenling Wang Jingjing Liu Shuaiyao Lu Zhenzhen Zhan Yufei Wang Ruhan A Wen Wang Peihua Niu Li Zhao Shiqiang Li Xiaopin Ma Luyao Zhang Yujian Zhang Weiguo Yao Xingjie Liang Jincun Zhao Zhongmin Liu Xiaozhong Peng Hangwen Li Wenjie Tan | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 3 |
| 10 | Production of heavy neutron-rich nuclei with radioactive beams in multinucleon transfer reactions显示文摘The production mechanism of heavy neutronrich nuclei is investigated by using the multinucleon transfer reactions of ^(136;148)Xe+^(208)Pb and ^(238)U+^(208)Pb in the framework of a dinuclear system model. The evaporation residual cross sections of target-like fragments are studied with the reaction system ^(148)Xe+^(208)Pb at near barrier energies. The results show that the final isotopic production cross sections in the neutron-deficient side are very sensitive to incident energy while it is not sensitive in the neutron-rich side. Comparing the isotopic production cross sections for the reactions of ^(208)Pb bombarded with stable and radioactive projectiles, we find that neutron-rich radioactive beams can significantly increase the production cross sections of heavy neutron-rich nuclei. | Cheng Li Peiwei Wen Jingjing Li Gen Zhang Bing Li Feng-Shou Zhang | 2017 | Nuclear Science and Techniques2017，28，8： | 2 |
| 11 | Oxidation of estrone by permanganate: Reaction kinetics and estrogenicity removal显示文摘Permanganate was used as an oxidant to control estrone in the present study. Kinetics was determined at pH 2.5-9.4 and temperature 15–40°C for the reaction of estrone with potassium permanganate. It was found that the reaction is second-order overall and first-order with respect to both estrone and permanganate. The second-order rate constant for the reaction at pH 5.8 and 25°C is 44.45 L mol-1 s-1. The reaction rate first decreased with the increase of pH in the range of 2.5-6.6 and then increased greatly with the increase of pH in the range of 6.6-9.4. In addition, the rate constant exponentially increased with the increase of reaction temperature. Removal of estrogenicity was also investigated during the degradation of estrone using yeast estrogen screen (YES). Results show that the estrogenicity increased in the initial 15 min of reaction and then decreased fast, with a removal rate of 73.8% within the 30 min of reaction. Results also demonstrate that the reaction rate between estrone and permanganate is faster in natural water background than in the ultra-pure water system. Permanganate oxidation is therefore a feasible option for removal of estrone in drinking water treatment processes. However, the contact time must be enough in order to remove estrone without causing the increase of estrogenicity. | XiaoLing Shao Jun Ma Gang Wen JingJing Yang | 2010 | Chinese Science Bulletin2010，55，9： | 2 |
| 12 | The mechanism of dentine hypersensitivity:Stimuli-induced directional cation transport through dentinal tubules显示文摘Dentine hypersensitivity is an annoying worldwide disease,yet its mechanism remains unclear.The long-used hydrodynamic theory,a stimuli-induced fluid-flow process,describes the pain processes.However,no experimental evidence supports the statements.Here,we demonstrate that stimuli-induced directional cation transport,rather than fluid-flow,through dentinal tubules actually leads to dentine hypersensitivity.The in vitro/in vivo electro-chemical and electro-neurophysiological approaches reveal the cation current through the nanoconfined negatively charged dentinal tubules coming from external stimuli(pressure,pH,and temperature)on dentin surface and further triggering the nerve impulses causing the dentine hypersensitivity.Furthermore,the cationic-hydrogels blocked dentinal tubules could significantly reduce the stimuli-triggered nerve action potentials and the anionhydrogels counterpart enhances those,supporting the cation-flow transducing dentine hypersensitivity.Therefore,the inspired ion-blocking desensitizing therapies have achieved remarkable pain relief in clinical applications.The proposed mechanism would enrich the basic knowledge of dentistry and further foster breakthrough initiatives in hypersensitivity mitigation and cure. | Nuo Chen Jingjing Deng Shengjie Jiang Xiang-Yu Kong Teng Zhou Kai Zhao Zuohui Xiao Huimin Zheng Weipeng Chen Congcong Zhu Xinyu Liu Liping Wen Yan Wei Xuliang Deng Lei Jiang | 2023 | Nano Research2023，16，1： | 2 |
| 13 | Chronic heat stress induces the disorder of gut transport and immune function associated with endoplasmic reticulum stress in growing pigs显示文摘Although high temperatures influence gut health,data on underlying mechanisms remains scant.Using a pig model,this study performed a global analysis on how chronic heat stress affects the transport and immune function of the gut through transcriptome,proteome,microbial diversity and flow cytometry.A total of 27 pigs with similar body weights were assigned into 3 groups,control(Con)group(23℃),chronic heat stressed(HS)group(33°C),and pair-fed(PF)group,in a controlled environment for 21 days.Our results showed that pigs in the HS group had reduced growth performance and diminished height of ileal villi(P<0.01).Transcriptome and proteome analyses demonstrated notable modification of expression of nutrients and ion transport-related transporters and gut mechanical barrier-related genes by chronic heart stress(P<0.05),suggesting damage of transport functions and the gut barrier.Chronic heat stress-induced endoplasmic reticulum stress also increased the synthesis of misfolded proteins,leading to upregulation of misfolded protein degradation and synthesis,as well as vesicle transport disorder(P<0.05).Energy supply processes were enhanced in the mitochondrion(P<0.05)to maintain biological processes with high energy demands.Furthermore,chronic heat stress activated complement cascade response-related genes and proteins in the gut mucosa(P<0.05).Our flow cytometry assays showed that the proportion of gut lymphocytes(CD4^(+)T cells,T cells,B cells in Peyer’s patch lymphocytes and CD4^(+)CD25^(+)T cells in intraepithelial lymphocytes)were significantly altered in the HS group pigs(P<0.05).In addition,the occurrence of gut microbial dysbiosis in the HS group pigs was characterized by increased potential pathogens(e.g.,Asteroleplasma,Shuttleworthia,Mycoplasma)and suppression of beneficial bacteria(e.g.,Coprococcus and Aeriscardovia),which are associated with gut immune function.Altogether,our data demonstrated that chronic heat stress induced gut transport and immune function disorder associated with endoplasmic reticulum stress in growing pigs. | Shanlong Tang Jingjing Xie Wei Fang Xiaobin Wen Chang Yin Qingshi Meng Ruqing Zhong Liang Chen Hongfu Zhang | 2022 | Animal Nutrition2022，，4： | 2 |
| 14 | Genetic relationship and pedigree of Chinese watermelon varieties based on diversity of perfect SNPs显示文摘Watermelon(Citrullus lanatus)is one of the world’s most important fruit crops,and China produces the most watermelons in the world.Recently,a watermelon variome consisting of 414 key resequenced accessions was reported.However,the genetic relationships and pedigree of Chinese watermelon varieties in the seed market remain unclear.In this study,241 evenly distributed perfect single nucleotide polymorphisms(SNPs)derived from the watermelon variome were selected for variety identification.The diversity of 247 Chinese watermelon varieties was identified based on their SNP genotypes.The 247 watermelon varieties were clustered into five subpopulations:the East Asian ecotype,intermediate ecotype,small fruit with red flesh ecotype,small fruit with yellow flesh ecotype,and American ecotype.We further established the pedigree of four subpopulations,of which JingXinNo.1,ZaoChunHongYu,HuangXiaoYu and XiaoLan,and Sugarlee were the main doner of the East Asian ecotype,small fruit with red flesh ecotype,small fruit with yellow flesh ecotype,and American ecotype,respectively.Thirty-two core SNPs were selected and applied in watermelon variety identification.They were also validated by the Kompetitive allele-specific PCR(KASPar)platform.The present study furthered our understanding of the genetic relationships and pedigree of watermelon varieties in China,and will help to manage the plant variety protection in watermelon. | Jingjing Yang Jian Zhang Hushan Du Hong Zhao Aijun Mao Xiaofei Zhang Luo Jiang Haiying Zhang Changlong Wen Yong Xu | 2022 | Horticultural Plant Journal2022，8，4： | 2 |
| 15 | Dysregulated adaptive immune response contributes to severe COVID-19显示文摘Dear Editor,The outbreak of the new coronavirus 5ARS-CoV-2 has resulted in a global pandemic.Due to the lack of a specific drug against this virus,the current clinical management of this disease mainly depends on supportive care to reduce inflammatory responses and to keep the lung functioning1. | Kuai Yu Jingjing He Yongjian Wu Baosong Xie Xuefei Liu Bo Wei Haibo Zhou Bingliang Lin Zhixiang Zuo Wen Wen Wenxiong Xu Bin Zou Lai Wei Xi Huang Penghui Zhou | 2020 | Cell Research2020，30，9： | 1 |
| 16 | A Cross-Layer Cooperative Jamming Scheme for Social Internet of Things显示文摘In this paper,we design a friendly jammer selection scheme for the social Internet of Things(IoT).A typical social IoT is composed of a cellular network with underlaying Device-to-Device(D2D)communications.In our scheme,we consider signal characteristics over a physical layer and social attribute information of an application layer simultaneously.Using signal characteristics,one of the D2D gadgets is selected as a friendly jammer to improve the secrecy performance of a cellular device.In return,the selected D2D gadget is allowed to reuse spectrum resources of the cellular device.Using social relationship,we analyze and quantify the social intimacy degree among the nodes in IoT to design an adaptive communication time threshold.Applying an artificial intelligence forecasting model,we further forecast and update the intimacy degree,and then screen and filter potential devices to effectively reduce the detection and calculation costs.Finally,we propose an optimal scheme to integrate the virtual social relationship with actual communication systems.To select the optimal D2D gadget as a friendly jammer,we apply Kuhn-Munkres(KM)algorithm to solve the maximization problem of social intimacy and cooperative jamming.Comprehensive numerical results are presented to validate the performance of our scheme. | Yan Huo Jingjing Fan Yingkun Wen Ruinian Li | 2021 | Tsinghua Science and Technology2021，26，4： | 1 |
| 17 | Selective separation of biobutanol from acetone–butanol–ethanol fermentation broth by means of sorption methodology based on a novel macroporous resin显示文摘 | Xiaoqing Lin Jinglan Wu Xiaohong Jin Jiansheng Fan Renjie Li Qingshi Wen Wenbin Qian Dong Liu Xiaochun Chen Yong Chen Jingjing Xie Jianxin Bai Hanjie Ying | 2012 | Biotechnol Progress2012，，4： | 1 |
| 18 | Comparison of DUS testing and SNP fingerprinting for variety identification in cucumber显示文摘Variety identification plays an important role in protecting the intellectual property of varieties,ensuring seed quality,and encouraging breeding innovation.Currently,morphological evaluation in the field,such as distinctness,uniformity,and stability(DUS)testing,and DNA fingerprinting in the laboratory using molecular markers are two dominant methods used for variety identification.Few studies have compared the results of these approaches,and the relationship between the two methods is obscure.In this study,134 dominant cucumber varieties were evaluated using 50 DUS testing traits and genotyped by 40 single nucleotide polymorphisms(SNPs).The 40 SNPs were developed in our previous study and arewell suited for variety identification.In the DUS testing,significant positive or negative correlations among 50 DUS traits were observed,and 20 core traits,including 15 fruit traits,were further selected to increase field inspection efficiency.This suggested that fruit shape plays an important role in variety identification.The ratio of fruit length/diameter was themost important trait,explaining 9.2%of the phenotypic variation.In the DNA fingerprinting test,the 40 SNPs were highly polymorphic and could distinguish all of the 134 cucumber varieties,and 14 core SNPs were selected to improve the identification rate.Interestingly,the population structure analysis of 134 cucumber varieties by phenotypic data in the DUS test was in accordance with the genotypic data from the DNA fingerprinting,indicating that all varieties could be divided into the same four subgroups:European type,North China type,South China type,and hybrids of the North China and South China types.Moreover,linear correlativity of distinguishment for each pair of varieties was observed between the DUS test and the DNA fingerprinting.These results indicated that these two methods have good application in future research,especially for the scaled-up analysis of hundreds of varieties. | Jian Zhang Jingjing Yang Shenzao Fu Jun Ren XiaoFei Zhang Changxuan Xia Hong Zhao Kun Yang Changlong Wen | 2022 | Horticultural Plant Journal2022，8，5： | 1 |
| 19 | Ultra- sound field distribution and ultrasonic oxidation desulfu- rization efficiency 显示文摘 | LIU Liyan WEN Jingjing YANG Yang | 2013 | Ultrason Sonochem2013，20，2： | 1 |
| 20 | Replication,pathogenicity,and transmission of SARS-CoV-2 in minks显示文摘Minks are raised in many countries and have transmitted severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)to humans.However,the biologic properties of SARS-CoV-2 in minks are largely unknown.Here,we investigated and found that SARS-CoV-2 replicates efficiently in both the upper and lower respiratory tracts,and transmits efficiently in minks via respiratory droplets;pulmonary lesions caused by SARS-CoV-2 in minks are similar to those seen in humans with COVID-19.We further found that a spike protein-based subunit vaccine largely prevented SARS-CoV-2 replication and lung damage caused by SARS-CoV-2 infection in minks.Our study indicates that minks are a useful animal model for evaluating the efficacy of drugs or vaccines against COVID-19 and that vaccination is a potential strategy to prevent minks from transmitting SARS-CoV-2. | Lei Shuai Gongxun Zhong Quan Yuan Zhiyuan Wen Chong Wang Xijun He Renqiang Liu Jinliang Wang Qinjian Zhao Yuxiu Liu Ningning Huo Junhua Deng Jingjing Bai Hongchao Wu Yuntao Guan Jianzhong Shi Kegong Tian Ningshao Xia Hualan Chen Zhigao Bu | 2021 | National Science Review2021，8，3： | 1 |
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