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您的检索式：作者名="Ioannis P. TROUGAKOS"
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| 1 | Regulation of clusterin/apolipoprotein J, a functional homologue to the small heat shock proteins, by oxidative stress in ageing and age-related diseases显示文摘 | Ioannis P. Trougakos Efstathios S. Gonos | 2006 | Free Radical Research2006，，12： | 2 |
| 2 | Ectopic expression of clusterin/apolipoprotein J or Bcl-2 decreases the sensitivity of HaCaT cells to toxic effects of ropivacaine显示文摘Local anesthetics inhibit cell proliferation and induce apoptosis in various cell types. Ropivacaine, a unique, novel tertiary amine-type anesthetic, was shown to inhibit the proliferation of several cell types including keratinocytes. We found that Ropivacaine could inhibit the proliferation and induce apoptosis in an immortalized human keratinocyte line, HaCaT, in a dose- and time-dependent manner and with the deprivation of serum. The dose-dependent induction of apoptosis by ropivacaine was demonstrated by DNA fragmentation analysis and the proteolytic cleavage of a caspase-3 substrate — poly (ADP-ribose) polymerase (PARP). In addition, ropivacaine downregulated the expression of clusterin/ apoliporotein J, a protein with anti-apoptotic properties, in a dose-dependent manner, which well correlated with the induction of apoptosis of HaCaT cells. To investigate the role of clusterin/apoliporotein J in ropivacaine-induced apoptosis, HaCaT cells overexpressing clusterin/apoliporotein J were generated and compared to cells expressing the well estab- lished anti-apoptotic Bcl-2 protein. Ectopic overexpression of the secreted form of clusterin/apoliporotein J or Bcl-2 decreased the sensitivity of HaCaT cells to toxic effects of ropivacaine as demonstrated by DNA fragmentation, the proteolytic cleavage of PARP and by a reduction in procaspase-3 expression. Furthermore, the downregulation of endogenous clusterin/apolipoprotein J levels by ropivacaine suggested that this might be one mechanism by which ropivacaine induced cell death in HaCaT cells. In conclusion, the ability of ropivacaine to induce antiproliferative responses and to suppress the expression of the anti-apoptotic protein clusterin/apolipoprotein J, combined with previ- ously reported anti-inflammatory activity and analgesic property of the drug, suggests that ropivacaine may have poten- tial utility in the local treatment of tumors. | Evangelos KONTARGIRIS Evangelos KOLETTAS Athina VADALOUCA Ioannis P. TROUGAKOS Efstathios S. GONOS Vasiliki KALFAKAKOU | 2004 | Cell Research2004，14，5： | 2 |
| 3 | Differential effects of clusterin/apolipoprotein J on cellular growth and survival显示文摘 | Ioannis P. Trougakos Magda Lourda Georgia Agiostratidou Dimitris Kletsas Efstathios S. Gonos | 2004 | Free Radical Biology and Medicine2004，，4： | 1 |
| 4 | Transcriptional and posttranslational regulation of clusterin by the two main cellular proteolytic pathways显示文摘 | Eirini Balantinou Ioannis P. Trougakos Niki Chondrogianni Lukas H. Margaritis Efstathios S. Gonos | 2009 | Free Radical Biology and Medicine2009，，9： | 1 |
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