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| 1 | 饲料中大豆油替代鱼油对大西洋鲑肝细胞脂肪酸代谢的影响显示文摘为探讨大豆油替代鱼油对大西洋鲑(Salmo salar L.)肝细胞脂肪酸代谢的影响,本试验分别以鱼油和大豆油为脂肪源配制2种试验饲料,并分别饲喂大西洋鲑幼鲑5个月。饲养结束后,分离和培养大西洋鲑肝细胞,一方面将细胞与碳14标记油酸([1-14C]OA)共孵育,然后测定细胞和细胞内各脂质成分以及脂肪酸β-氧化产物的放射活性;另一方面提取肝细胞总RNA,采用实时荧光定量聚合酶链式反应(RT-qPCR)测定脂肪酸结合蛋白3(FABP3)、脂肪酸结合蛋白10(FABP10)、Δ5去饱和酶(D5D)、Δ6去饱和酶(D6D)及肉碱棕榈酰转移酶Ⅰ(CPT-Ⅰ)基因的相对表达量。结果表明:1)鱼油组与大豆油组肝细胞内游离脂肪酸(FFA)放射活性百分率分别为(62.7±13.0)%和(55.9±9.1)%,2组间无显著差异(P>0.05);鱼油组与大豆油组肝细胞内磷脂(PLs)放射活性百分率分别为(20.9±8.7)%和(17.9±5.4)%,2组间无显著差异(P>0.05);鱼油组肝细胞内三酰甘油(TAG)放射活性百分率为(2.0±0.9)%,显著高于大豆油组的(0.8±0.4)%(P<0.05);鱼油组与大豆油组肝细胞内β-氧化产物放射活性百分率分别为(1.0±0.3)%和(1.0±0.2)%,2组间无显著差异(P>0.05)。2)大豆油组肝细胞中FABP3、D6D、D5D基因的相对表达量均显著高于鱼油组(P<0.05),而FABP10和CPT-Ⅰ基因的相对表达量在2组间无显著差异(P>0.05)。由此可见,大豆油替代饲料中的鱼油对大西洋鲑肝细胞内FFA、PLs合成及脂肪酸β-氧化等脂肪酸代谢途径无显著影响,但显著降低了肝细胞内TAG的合成,促进了肝细胞内脂肪酸的去饱和,因此大豆油替代鱼油在一定程度上影响了肝细胞内脂肪酸代谢。 | 周继术 TORSTENSEN Bente Elisabeth STUBHAUG Ingunn 吉红 | 2013 | 动物营养学报2013，25，11： | 4 |
| 2 | 饲料中植物油替代鱼油对大西洋鲑肝细胞油酸跨膜吸收的影响显示文摘以[1-14C]油酸(oleic acid;18:1n-9,OA)为指示剂,研究了不同饲料油源饲喂下大西洋鲑肝细胞膜脂肪酸组成受到改变时该细胞对OA吸收的状况,以探讨植物油(Vegetable oil,VO)替代鱼油(Fish oil,FO)对大西洋鲑肝细胞脂肪酸跨膜吸收的影响,为大西洋鲑饲料中植物油替代鱼油的可行性提供理论依据。试验先以鱼油和大豆油为油源配制两种全价配合饲料,分别饲喂大西洋鲑幼鲑5个月,使其产生不同的脂肪酸组成。在饲养结束后,分离并培养试验鱼肝细胞,将细胞与[1-14C]OA及37.5μmol/L OA(1/30,mol/mol,0.3μCi/瓶)共孵育2h,收集并测定细胞内OA放射活性,再计算细胞内OA吸收量[nmol/(h·million cells)]。同时,试验采取RT-PCR方法测定了细胞脂肪酸运送蛋白(Fatty acid transport protein,FATP)、脂肪酸移位蛋白(Fatty acid translocase,FAT/CD36)的基因表达量。结果表明,FO和VO组肝细胞对OA吸收分别为(0.924±0.258)及(0.888±0.179)nmol/(h·million cells),两组间无显著差异(P>0.05)。RT-PCR的检测结果表明,FAT/CD36和FATP基因表达量在FO与VO两组间均无显著差异(P>0.05)。结果表明,从植物油替代鱼油不影响大西洋鲑肝细胞对长链脂肪酸的跨膜吸收方面来看,大西洋鲑饲料中以植物油替代鱼油具有可行性。 | 周继术 Bente E.Torstensen Ingunn Stubhaug | 2014 | 水生生物学报2014，38，1： | 4 |
| 3 | Post-weaning and whole-of-life performance of pigs is determined by live weight at weaning and the complexity of the diet fed after weaning显示文摘The production performance and financial outcomes associated with weaner diet complexity for pigs of different weight classes at weaning were examined in this experiment. A total of 720 weaner pigs(360 entire males and 360 females) were selected at weaning(27 ± 3 d) and allocated to pens of 10 based on individual weaning weight(light weaning weight: pigs below 6.5 kg; medium weaning weight: 6.5 to8 kg; heavy weaning weight: above 8.5 kg). Pens were then allocated in a 3 x 2 x 2 factorial arrangement of treatments with the respective factors being weaning weight(heavy, medium and light; H, M and L,respectively), weaner diet complexity(high complexity/cost, HC; low complexity/cost, LC), and gender(male and female). Common diets were fed to both treatment groups during the final 4 weeks of the weaner period(a period of 39 days). In the first 6 d after weaning, pigs offered the HC diets gained weight faster and used feed more efficiently than those offered the LC diets(P = 0.031). Pigs fed a HC diet after weaning tended to be heavier at the sale live weight of 123 d of age compared with pigs fed the LC diet(P = 0.056). There were no other main effects of the feeding program on growth performance through to slaughter. Weaning weight had a profound influence on lifetime growth performance and weight at 123 d of age, with H pigs at weaning increasing their weight advantage over the M and L pigs(101.3,97.1, 89.6 kg respectively, P < 0.001). Cost-benefit analyses suggested there was a minimal benefit in terms of cost per unit live weight gain over lifetime when pigs were offered a HC feeding program to L,with a lower feed cost/kg gain. The results from this investigation confirm the impact of weaning weight on lifetime growth performance, and suggest that a HC feeding program should be focused on L weaner pigs(i.e., weaning weight less than 6.5 kg at 27 d of age) in order to maximise financial returns. | Cherie L.Collins John R.Pluske Rebecca S.Morrison Trevor N.McDonald Robert J.Smits David J.Henman Ingunn Stensland Frank R.Dunshea | 2017 | Animal Nutrition2017，，4： | 3 |
| 4 | Drivers of food SMEs network success:101 tales from Norway显示文摘 | Nina Veflen Olsena Ingunn Elvekrok Etty Ragnhild Nilsen | | 0，，： | 1 |
| 5 | Sustainable SME practice:A reflection on supply-chain environmental manage-ment intervention显示文摘 | Nina Veflen Olsena Ingunn Elvekrok Etty Ragnhild Nilsen | | 0，，： | 1 |
| 6 | Application of LCA as a decision-making tool for waste management systems显示文摘 | Cecilia Askham Nyland Ingunn Saur Modahl Hanne Lerche Raadal Ole J?rgen Hanssen | 2003 | The International Journal of Life Cycle Assessment2003，，6： | 1 |
| 7 | Comparison of ELISA and LC-MS analyses for yessotoxins in blue mussels (Mytilus edulis) 显示文摘 | INGUNN A SAMDAL JOHN A B AASEN LYN R BRIGGS | 2005 | Toxicon2005，46，： | 1 |
| 8 | Thermal stability of glucose and other sugar aldoses in normal phase high performance liquid chromatography显示文摘 | Rune Slimestad Ingunn M. V?gen | 2006 | Journal of Chromatography A2006，，2： | 1 |
| 9 | Diumal temperature ahemations (DIF/drop)affect chlorophyll content and chlorophyll a/ chlorophyll b ratio in Melissa officinalis L and Ocimum basilicum L but not in Viola x wittrockiana Gains显示文摘 | Ingunn M V Roar M Eli R | 2003 | Science Horticult2003，97，： | 1 |
| 10 | The formation and permeability of drugs across free pectin and chitosan films prepared by a spraying method显示文摘 | Hiorth Marianne Tho Ingunn Sande Sverre Arne | 2003 | European Journal of Pharmaceutics and Biopharmaceutics2003，，56： | 1 |
| 11 | The Natural Environment as a Playground for Children: The Impact of Outdoor Play Activities in Pre-Primary School Children显示文摘 | Ingunn Fj?rtoft | 2001 | Early Childhood Education Journal2001，，2： | 1 |
| 12 | Genomic organization and transcript profiling of the bovine toll-like receptor gene cluster TLR6 - TLR1 - TLR10显示文摘 | Monica Aa. Opsal Dag Inge V?ge Ben Hayes Ingunn Berget Sigbj?rn Lien | 2006 | Gene2006，，： | 1 |
| 13 | Multivariate analysis of relationships between material properties, process parameters and tablet tensile strength for a-lactose m0nohydrates 显示文摘 | Rahul V H Ingunn T Annette B B | 2009 | Eur J Pharm Biopharm2009，73，： | 1 |
| 14 | Human Rights, Formalization and Women's Land Rights in Southern and Eastern Africa显示文摘 | Ingunn Ikdal Anne Helium etc | 2005 | Stud- ies in Women''s Law2005，，57： | 1 |
| 15 | Cold technologies versus warm care? On affective and social relations with and through care technologies显示文摘 | Jeannette Pols Ingunn Moser | 2009 | Alter - European Journal of Disability research, Revue européen de recherche sur le handicap2009，，2： | 1 |
| 16 | Environmental assessment of chemical products from a Norwegian biorefinery显示文摘 | INGUNN S M ANDREAS B CLARA V | 2015 | Journal of Cleaner Production2015，94，： | 1 |
| 17 | Comparison of iron isotope variations in modern and Ordovician siliceous Fe oxyhydroxide deposits显示文摘 | Kirsten Moeller Ronny Schoenberg Tor Grenne Ingunn H. Thorseth Kerstin Drost Rolf B. Pedersen | 2014 | Geochimica et Cosmochimica Acta2014，，： | 1 |
| 18 | A DNA vaccine directed against a rainbow trout rhabdovirus induces early protection against a nodavirus chanllenge in turbot 显示文摘 | Ingunn S Lorenzen E Lorenzen N | 2003 | Vaccine2003，21，： | 1 |
| 19 | Pathophysiological roles of osteoprotegerin (OPG)显示文摘 | Penny Reid Ingunn Holen | 2009 | European Journal of Cell Biology2009，88，1： | 1 |
| 20 | The natural environment as a playground for children Landscape description and analyses of a natural playground显示文摘 | Ingunn Fjortoft Jostein Sageie | 2000 | Landscape and Urban Planning2000，，48： | 1 |
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