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| 1 | Characterization and phylogenetic analysis of novel polyene type antimicrobial metabolite producing actinomycetes from marine sediments:Bay of Bengal India显示文摘Objective:To isolate and indentify the promising antimicrobial metabolite producing Streptomyces strains from marine sediment samples from Andraprudesh coast of India.Methods:Antagonistic aetinomycetes were isolated by starch casein agar medium and modified nutrient agar medium with 1%glucose used as a base for primary screening.Significant antimicrobial metabolite producing strains were selected and identified by using biochemical and 16S rDNA level.Minimum inhibitory concentrations of the organic extracts were done by using broth micro dilution method.Results:Among the 210 actinomyeetes,64.3%exhibited activity against Gram positive bacteria,48.5%showed activity towards Cram negative bacteria,38.8%exhibited both Cram positive and negative bacteria and 80.85%isolates revealed significant antifungal activity.However,five isolates AP-5,AP-18,AP-41 and AP-70 showed significant antimicrobial activity.The analysis of cell wall hydrolysates showed the presence of LL-diaminopimelic acid and glycine in all the isolates.Sequencing analysis indicated that the isolates shared 98.5%-99.8%sequence identity to the 16S rDNA gene sequences of the Streptomyces taxons.The antimicrobial substances were extracted using hexane and ethyl acetate from spent medium in which strains were cultivated at 30X for five days.The antimicrobial activity was assessed using broth micro dilution technique.Each of the culture extracts from these five strains showed a typical polyenelike property.The lowest minimum inhibitory concentrations of ethyl acetate extracts against Escherichia coli and Cumularia lunula were 67.5 and 125.0μg/mL,respectively.Conclusions:It can be concluded that hexane and ethyl acetate soluble extracellular products of novel isolates are effective against pathogenic bacteria and fungi. | Valan Arasu M Asha KRT Duraipandiyan V Ignacimuthu S Agastian P | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，10： | 5 |
| 2 | Antibacterial activity of some actinomycetes from Tamil Nadu,India显示文摘Objective:To isolate novel actinomycetes and to evaluate their antibacterial activity.Methods:Three soil samples were collected from Vengodu(village)in Kanchipuram district,Tamil Nadu,India.Actinomycetes were isolated using serial dilution and plating method on actinomycetes isolation agar.Results:Totally 35 isolates were obtained on the basis of colony characteristics on actinomycetes isolation agar.All the isolates were screened for antibacterial activity by cross streak method.Medium and optimization of day were done for the potent strains using Nathan's agar well diffusion method.Isolation of bioactive compounds from significant active isolates was done by using different media.The most active isolate VAS 10 was identified as Actinobacterium Loyola PBT VAS 10(accession No.JF501398)using 16s rRNA sequence method.The hexane,ethyl acetate,dichloromethane and butanol extracts of VAS 10 were tested against bacteria.The maximum antibacterial activity was observed in dichloromethane and ethyl acetate;maximum zones of inhibition were observed against Enterococcus durans.The rRNA secondary structure and the restriction sites of Actinobacterium Loyola VAS 10 were predicted using Genebee and NEBCutter online tools respectively.Conclusions:The present study showed that among the isolated actinomycetes,Actinobacterium Loyola PBT VAS 10(accession No.JF501398)showed good antibacterial activity against the tested bacteria. | Pachaiyappan Saravana Kumar John Poonga Preetam Raj Veeramuthu Duraipandiyan Savarimuthu Ignacimuthu | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，12： | 4 |
| 3 | Phytochemical analysis and in vitro antimicrobial activity of Illicium griffithii Hook. f. & Thoms extracts显示文摘 | A Vijayakumar V Duraipandiyan B Jeyaraj P Agastian M Karunai Raj S Ignacimuthu | 2012 | Asian Pacific Journal of Tropical Disease2012，，3： | 4 |
| 4 | A novel compound from Casearia esculenta (Roxb.) root and its effect on carbohydrate metabolism in streptozotocin-diabetic rats显示文摘 | Govindasamy Chandramohan Savarimuthu Ignacimuthu Kodukkur Viswanathan Pugalendi | 2008 | European Journal of Pharmacology2008，，1： | 3 |
| 5 | Antimicrobial activity of Streptomyces spp. ERI-26 recovered from Western Ghats of Tamil Nadu显示文摘 | M. Valan Arasu V. Duraipandiyan P. Agastian S. Ignacimuthu | 2008 | Journal de Mycologie Médicale2008，，3： | 2 |
| 6 | Antidiabetic and antilipidemic effect of eremanthin from Costus speciosus (Koen.)Sm., in STZ-induced diabetic rats显示文摘 | J. Eliza P. Daisy S. Ignacimuthu V. Duraipandiyan | 2009 | Chemico-Biological Interactions2009，，1： | 2 |
| 7 | Cytotoxic (A549) and antimicrobial effects of Methylobacterium sp.isolate (ERI-135) from Nilgiris forest soil,India显示文摘Objective:To assess the antimicrobial and cytotoxic effects of Methylobacterium sp.isolated from soil sample of Doddabetta forest,Nilgiris,Western Ghats of Tamil Nadu.Methods:Isolation of Methylobacterium was performed from soils by serial dilution plate technique.The strain was grown in modified nutrient gulucose agar(MNGA)medium to study the morphology and biochemical characteristics.Methylobacterium sp.was screened for its antimicrobial activity against pathogenic bacteria and fungi.The strain was subjected to 16S rRNA analysis and was identified as Methylohacterium sp.The nucleotide sequence of the 16S rRNA gene of the isolate exhibited close similarity with other Methylobacterium sp.and has been submitted to Genbank.The antibacterial substances were extracted using chloroform and ethyl acetate from MNGA medium in which ERI-135 had grown for 5 d at 30℃.Cytotoxic effect was also studied.GC-MS analysis was carried out.The antimicrobial activity was assessed using broth micro dilution technique.Results:Ethyl acetate extract showed activity against bacteria such as Bacillus subtilis,Klebsiella pneumoniae(K.pneumoniae),Pseudomonas aeruginosa,Salmonella typkimurium,Shigella flexneri,Enterobacter aerogenes,Staphylococcus aureu and Staphylococcus epidermidis(S.epidermidis)and fungi such as,Candida albicans and Trichophyton rubrum.The lowest minimum inhibitory concentrations were:250μg/mL against 5.epidermidis and 250μg/mL against K.pneumonia.The isolate had the ability to produce enzymes such as protease.The exyract showed cytotoxic effect in human adenocarcinoma cancer cell line(A549).GC-MS analysis showed the presence of isovaleric acid(3.64%),2-Methylbulanoic acid(5.03%),isobutyramide(5.05%),N,Noimethylformamide-di-t-butylacetal(9.79%),benzeneacetamide(15.56%),octyl butyl phthalate(3.59%)and diisooctyl phthalate(5.79)in the extract.Conclusions:Methylobaeterium sp.(ERI-135)showed promising antibacterial and cytotoxic activity.This is the first report in the antimicrobial and cytotoxic effect of Methylobaeterium sp. | Balachandran C Duraipandiyan V Ignacimuthu S | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，9： | 2 |
| 8 | Swertiamarin attenuates inflammation mediators via modulating NF-κB/I κB and JAK2/STAT3 transcription factors in adjuvant induced arthritis显示文摘 | S. Saravanan V.I. Hairul Islam N. Prakash Babu P. Pandikumar K. Thirugnanasambantham M. Chellappandian C. Simon Durai Raj M. Gabriel Paulraj S. Ignacimuthu | 2014 | European Journal of Pharmaceutical Sciences2014，，： | 2 |
| 9 | Antidiabetic effect of Symplocos cochinchinensis (Lour.) S. Moore. in type 2 diabetic rats显示文摘 | Christudas Sunil Savarimuthu Ignacimuthu Paul Agastian | 2010 | Journal of Ethnopharmacology2010，，2： | 2 |
| 10 | MicroRNA in prostate cancer 显示文摘 | Khanmi K Ignacimuthu S Paulraj MG | 2015 | Clin Chim Acta2015，451，： | 1 |
| 11 | Hypoglycemic and antihyperglycemic effect of Begonia malabarica Lain 显示文摘 | Pandikumar P Babu N Prakash Ignacimuthu S | 2009 | Journal of Ethnopharmacology2009，24，1： | 1 |
| 12 | Antibacterial and antifungal activity of Flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam.显示文摘 | V. Duraipandiyan S. Ignacimuthu | 2009 | Journal of Ethnopharmacology2009，，3： | 1 |
| 13 | Anti-inflammatory activity of Albizia lebbeck Benth., an ethnomedicinal plant, in acute and chronic animal models of inflammation显示文摘 | N. Prakash Babu P. Pandikumar S. Ignacimuthu | 2009 | Journal of Ethnopharmacology2009，，2： | 1 |
| 14 | In vitro antimicrobial activity of Streptomyces spp. ERI-3 isolated from Western Ghats rock soil (India)显示文摘 | M. Valan Arasu V. Duraipandiyan P. Agastian S. Ignacimuthu | 2008 | Journal de Mycologie Médicale2008，，1： | 1 |
| 15 | Immunomodulatory,analgesic and antipyretic effects of violacein isolated from Chromobacterium violaceum 显示文摘 | Antonisamy P Ignacimuthu S | 2010 | Phytomedicine2010，17，6： | 1 |
| 16 | Plant regeneration through somatic embryogenesis from mature leaf explants of Eryngium foetidum,a condiment显示文摘 | IGNACIMUTHU S AROCKIASAMY S ANTONYSAMY M | 1999 | Plant Cell Tissue Organ Culture1999，56，： | 1 |
| 17 | In vitro antibacterial activity of some plant essential oils显示文摘 | Seenivasan Prabuseenivasan Manickkam Jayakumar Savarimuthu Ignacimuthu | 2006 | BMC Complementary and Alternative Medicine2006，，10： | 1 |
| 18 | Rna interference--a silent but an efficient therapeutic tool显示文摘 | Ramachandran PV Ignacimuthu S | 2013 | Appl Biochem Biotechnol2013，169，6： | 1 |
| 19 | RNAinterference as a plausible anticancer therapeutic tool 显示文摘 | RAMACHANDRAN P V IGNACIMUTHU S | 2012 | Asian Pac J Cancer Prev2012，13，6： | 1 |
| 20 | Normo-glycemic and hypolipidemic effect of costunolide isolated from Costus speciosus (Koen ex. Retz.)Sm. in streptozotocin-induced diabetic rats显示文摘 | James Eliza Pitchai Daisy Savarimuthu Ignacimuthu Veeramuthu Duraipandiyan | 2008 | Chemico-Biological Interactions2008，，2： | 1 |
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