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| 1 | Gadolinium-hyaluronic acid nanoparticles as an efficient and safe magnetic resonance imaging contrast agent for articular cartilage injury detection显示文摘Accurate detection of cartilage injuries is critical for their proper treatment because these injuries lack the selfhealing ability and lead to joint dysfunction.However,the low longitudinal T1 relaxivity(r1)and non-specificity of contrast agents(such as gadolinium(III)-diethylenetriamine-pentaacetic acid(Gd-DTPA))significantly limit the efficiency of clinical magnetic resonance imaging(MRI)applications.To overcome these drawbacks,we integrated hyaluronic acid(HA)with Gd to synthesize a Gd-DTPA-HA composite,which was subsequently freeze-dried to produce nanoparticles(NPs).The resultant Gd-HA NPs demonstrated a greater r1 value(12.51 mM^-1 s^-1)compared with the bulk Gd-DTPA-HA(8.37 mM^-1 s^-1)and clinically used Gd-DTPA(3.88 mM^-1 s^-1).Moreover,the high affinity of HA to the cartilage allowed these NPs to penetrate deeper beyond the cartilage surface.As a result,Gd-HA NPs considerably increased the quality of cartilage and lesion MR images via their intra-articular injection in vivo.Specifically,2 h after NP administration,the signal-to-noise ratio at the injured cartilage site was 2.3 times greater than the value measured before the injection.In addition,Gd-HA NPs exhibited good biosafety properties due to the absence of adverse effects in the blood or on the main organs.It was also showed that Gd NPs were first metabolized by the kidney and liver and then excreted from the body with urine.Thus,Gd-HA NPs can potentially serve as an efficient MRI contrast agent for improved detection of cartilage injuries. | Rong Lu Yuyang Zhang Hongyue Tao Lu Zhou Huidi Li Tianwu Chen Peng Zhang Yao Lu Shuang Chen | 2020 | Bioactive Materials2020，5，4： | 3 |
| 2 | Effect of Lamb Age and in vitro Culture System of Oocytes on JIVET Technology显示文摘[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i. p. injection of pregnant mare's serum gonadotropin( PMSG). The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200 μmol/L cysteine to the basal maturation solution. Then,the oocytes were fertilized in fertilization medium I containing 2% estrus sheep serum( ESS) or fertilization medium II containing 3 mg/ml bull serum albumin( BSA). Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined. [Results]The average number of oocytes recovered per lamb was( 111. 00 ± 16. 97),( 139. 50 ± 28. 99),( 108. 50 ± 17. 68) and( 42. 00 ± 11. 31) for5-,7-,8-and 10-week-old Dorper × small-tailed Han lambs,respectively. The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs( P < 0. 05),but there was no significant difference among 5-,7-and 8-week-old lambs( P > 0. 05). The maturation rate of oocytes cultured in modified maturation solution was 3. 64% higher than that in basal maturation solution. The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II( P < 0. 01). [Conclusions] The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium. | Yangyi MAO Huidi LUO Guanwu ZHANG Zhiwu WANG Peng ZHAO Jun LI Hongyu GUO Shenghua ZHOU Huihui GUO Chunyan LI | 2019 | Agricultural Biotechnology2019，8，1： | 3 |
| 3 | Blockchain Technology Empowers Telecom Network Operation显示文摘Telecom operators are deploying the fifth generation(5G) networks around the world which promises high information transmission rate, wide network coverage, low communication delay, and easy access to a large number of devices. However, during the construction and operation of 5G, telecom operators face many challenges, such as insufficient frequency resources, low efficiency of network management, information opacity, risks of data interoperability, and network privacy vulnerabilities. As 5G is generally deployed in heterogeneous networks with massive ubiquitous devices, it is quite necessary to provide secure and decentralized solutions. Blockchain is a distributed system maintained by multiple parties with inherit features including decentralization,traceability and tamperproof. These features collectively contribute to a new application ecosystem where transactions are secure and trustworthy. Therefore,blockchain is expected to integrate with 5G networks to build safer and more reliable mobile network infrastructures. This paper firstly discusses the merits of blockchain technology and the benefits of integrating it with 5G networks. Then a variety of applications of blockchain technology in telecom network operation are reviewed, including spectrum sharing, international roaming settlement, network operation management, number selection management and supply chain management. In the end, we show our recently advance in integrating blockchain in 5G by introducing a multi framework based blockchain service network architecture deployed in real environment. | Huidi Li Peng Gao Yi Zhan Min Tan | 2022 | China Communications2022，19，1： | 3 |
| 4 | JIVET Research on Texel Sheep显示文摘The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was conducted on 4-8-week old Texel sheep,and in-vivo ovum collection,in-vitro oocyte maturation and fertilization,and fertilized-embryo culture and transplantation were adopted to perform JIVET research on Texel sheep.The results showed that 296 available oocytes were obtained at an average of 74 oocytes per sheep;205 fertilized eggs were obtained at the 2-4 cell stage at an average of 51.25 eggs per sheep and a cleavage rate of 69.26%;and 66 of the fertilized eggs were transplanted to 11 receptor sheep.Six sheep were conceived at a conception rate of 54.55%,and 11 lambs were born.These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep. | Yangyi MAO Huidi LUO Xi LI Peng ZHAO Jun LI Sujun WU Hongyu GUO | 2020 | Agricultural Biotechnology2020，9，5： | 2 |
| 5 | Microstructure, mechanical and tribological properties of graphite-like amorphous carbon films prepared by unbalanced magnetron sputtering显示文摘 | Yongjun Wang Hongxuan Li Li Ji Fei Zhao Qinghua Kong Yongxia Wang Xiaohong Liu Weilong Quan Huidi Zhou Jianmin Chen | 2010 | Surface & Coatings Technology2010，，8： | 2 |
| 6 | Graphdiyne-based metal atomic catalysts for synthesizing ammonia显示文摘Development of novel catalysts for nitrogen reduction at ambient pressures and temperatures with ultrahigh ammonia(NH_(3))yield and selectivity is challenging.In this work,an atomic catalyst with separated Pd atoms on graphdiyne(Pd-GDY)was synthesized,which shows fascinating electrocatalytic properties for nitrogen reduction.The catalyst has the highest average NH_(3)yield of 4.45±0.30 mgNH_(3)mg Pd^(-1)h^(-1),almost tens of orders larger than for previously reported catalysts,and 100%reaction selectivity in neutral media.Pd-GDY exhibits almost no decreases in NH_(3)yield and Faradaic efficiency.Density functional theory calculations show that the reaction pathway prefers to perform at the(Pd,C1,C2)active area because of the strongly coupled(Pd,C1,C2),which elevates the selectivity via enhanced electron transfer.By adjusting the p–d coupling accurately,reduction of self-activated nitrogen is promoted by anchoring atom selection,and side effects are minimized. | Huidi Yu Yurui Xue Lan Hui Chao Zhang Yan Fang Yuxin Liu Xi Chen Danyan Zhang Bolong Huang Yuliang Li | 2021 | National Science Review2021，8，8： | 2 |
| 7 | Influence of substrate bias voltage on structure and properties of the CrAlN films deposited by unbalanced magnetron sputtering显示文摘 | Yanhong Lv Li Ji Xiaohong Liu Hongxuan Li Huidi Zhou Jianmin Chen | 2011 | Applied Surface Science2011，，8： | 1 |
| 8 | Composition, microstructure, and properties of CrN x films deposited using medium frequency magnetron sputtering显示文摘 | Qinghua Kong Li Ji Hongxuan Li Xiaohong Liu Yongjun Wang Jianmin Chen Huidi Zhou | 2010 | Applied Surface Science2010，，6： | 1 |
| 9 | Annealing effect on the structure, mechanical and tribological properties of hydrogenated diamond-like carbon films 显示文摘 | Hongxuan Li Chengbing Wang Jianmin Chen Huidi Zhou Huiwen Liu | 2006 | Thin Solid Films2006，515，： | 1 |
| 10 | Microstructure and mechanical properties of yttria-stabilized ZrO2/Al2O3 nanocomposite ceramics显示文摘 | Ye Yinping Li Jiangong Zhou Huidi | 2008 | Ceram Int2008，34，8： | 1 |
| 11 | Three new shRNA expression vectors targeting the CYP3A4 coding sequence to inhibit its expression显示文摘RNA interference(RNAi)is useful for selective gene silencing.Cytochrome P4503A4(CYP3A4),which metabolizes approximately 50% of drugs in clinical use,plays an important role in drug metabolism.In this study,we aimed to develop a short hairpin RNA(shRNA)to modulate CYP3A4 expression.Three new shRNAs(S1,S2 and S3)were designed to target the coding sequence(CDS)of CYP3A4,cloned into a shRNA expression vector,and tested in different cells.The mixture of three shRNAs produced optimal reduction(55%)in CYP3A4 CDS-luciferase activity in both CHL and HEK293 cells.Endogenous CYP3A4 expression in HepG2 cells was decreased about 50%at both mRNA and protein level after transfection of the mixture of three shRNAs.In contrast,CYP3A5 gene expression was not altered by the shRNAs,supporting the selectivity of CYP3A4 shRNAs.In addition,HepG2 cells transfected with CYP3A4 shRNAs were less sensitive to Ginkgolic acids,whose toxic metabolites are produced by CYP3A4.These results demonstrate that vector-based shRNAs could modulate CYP3A4 expression in cells through their actions on CYP3A4 CDS,and CYP3A4 shRNAs may be utilized to define the role of CYP3A4 in drug metabolism and toxicity. | Siyun Xu Yongsheng Xiao Li Li Lushan Yu Huidi Jiang Aiming Yu Su Zeng | 2014 | Acta Pharmaceutica Sinica B2014，4，5： | 0 |
| 12 | Magnetron sputtering NbSe_(2)film as lubricant for space current-carrying sliding contact显示文摘This study demonstrates that magnetron-sputtered NbSe_(2)film can be used as a lubricant for space current-carrying sliding contact,which accommodates both metal-like conductivity and MoS_(2)-like lubricity.Deposition at low pressure and low energy is performed to avoid the generation of the interference phase of NbSe_(3).The composition,microstructure,and properties of the NbSe_(2)films are further tailored by controlling the sputtering current.At an appropriate current,the film changed from amorphous to crystalline,maintained a dense structure,and exhibited excellent comprehensive properties.Compared to the currently available electrical contact lubricating materials,the NbSe_(2)film exhibits a significant advantage under the combined vacuum and current-carrying conditions.The friction coefficient decreases from 0.25 to 0.02,the wear life increases more than seven times,and the electric noise reduces approximately 50%. | Yang YANG Lulu PEI Hongzhang ZHANG Kai FENG Pengfei JU Wenshan DUAN Li JI Hongxuan LI Xiaohong LIU Huidi ZHOU Jianmin CHEN | 2023 | Friction2023，11，3： | 0 |
| 13 | Chinese herbal medicine for incomplete immune reconstruction in patients with AIDS undergoing antiretroviral treatment: A systematic review of randomized trials显示文摘Objective:To evaluate the effectiveness and safety of Chinese herbal medicines(CHMs)for incomplete immune reconstruction in patients with HIV/AIDS.Methods:Eight electronic databases were searched for randomized controlled trials(RCTs)on the use of CHM for patients with HIV/AIDS with incomplete immune reconstruction.Outcomes included CD4þcell count,quality of life,and adverse events/effects.The Cochrane Risk of Bias was employed to evaluate the methodological quality of the included RCTs.Results:We identified 13 eligible RCTs,with an overall high risk of bias,on 10 different CHMs.There was a significant increase in CD4þcell count after the use of Jianpi Yiqi medicinal paste for 3 months;tripterygium glycosides tablets(TGTs)for 3 months(mean difference[MD]52.63 cells/mL,95%confidence interval[CI,46.98,58.28]),6,9,and 12 months;Wenshen Jianpi granules for 6 months;Shenling Fuzheng capsules for 6 months(MD 49.53 cells/mL,95%CI[8.45,90.61])and 12 months;Aikeqing granules for 9 months(MD 61.51 cells/mL,95%CI[16.25,106.77])and 12 months;Guipi decoction for 12 months;Mianyi No.2 granules(JT)for 12 and 18 months;and Chinese medicine granules for 18 months.The increase in the mean difference of CD4þcell count from 6 to 18 months was larger in Chinese medicine granules and Mianyi No.2 granules(JT).Guipi decoction and Jianpi Qushi decoction improved the Karnofsky score.Four RCTs reported the outcome of adverse events/effects,while four cases of minor adverse effects were reported in the TGTs group.Conclusion:Jianpi Yiqi medicinal paste,Wenshen Jianpi granules,Shenling Fuzheng capsules,Aikeqing granules,Guipi decoction,and TGTs may be effective in increasing CD4þwithin 12 months,and Mianyi No.2 granules(JT)and Chinese medicine granules may show long-term effects.High-quality large RCTs on the effectiveness and safety of CHMs are still warranted. | Chen Shen Jing Li Yajun Lian Huidi Lan Fenglan Pu Wenjing Zhang Lingyao Kong Jianping Liu | 2021 | Journal of Traditional Chinese Medical Sciences2021，8，4： | 0 |
| 14 | Study on the Tribological Properties of Graphite-like Amorphous Carbon Film under Different Testing Conditions显示文摘Graphite-like amorphous carbon film was fabricated by unbalanced magnetron sputtering technique.Raman spectroscopy,atomic force microscopy(AFM)and tribometer were subsequently used to investigate the microstructure and tribological properties of the resultant film.It is found that the deposited carbon film is dominated by sp 2 sites,and the intensity ratio of the D and G peaks is as high as 4.0,which is one order of magnitude larger than that of diamond-like carbon films with high sp 3 content,indicating a more graphite-like structure.However,the as-deposited carbon film exhibits moderately high hardness(13.7 GPa),low internal stress(0.38 GPa)and superior tribological properties with high load bearing capacity(Hertz contact stress about 3.2 GPa)and low wear rate(2.73×10-10 cm3/N.m)in ambient atmosphere.Although it displays a poor wear resistance in water lubricated condition,a superior wear resistance is achieved in oil lubricated condition.Its inherent physical property,the formation of transfer layer and the friction induced chemical reactions may be commonly responsible for its tribological properties. | Wang Yongjun Li Hongxuan Ji Li Zhao Fei Liu Xiaohong Wu Yanxia Zhou Huidi Chen Jianmin | 2012 | 稀有金属材料与工程2012，41，S1： | 0 |
| 15 | Effect of Ti Incorporation on the Microstructure and Properties of the a-C:H Films Deposited by Magnetron Sputtering Technique显示文摘Amorphous hydrogenated carbon(a-C:H)and Ti-incorporated a-C:H(Ti/a-C:H)films were deposited by magnetron sputtering with a mixture gas of CH4 and Ar.The effect of the Ti incorporation on the chemical composition,microstructure and properties of the as-deposited and the annealed films were investigated by various techniques.It has been shown that the bonding structure and the internal stress were sensitive to the incorporation of the Ti atoms.The results of the XPS revealed that the concentration of Ti atom on the surface of the film increased when the Ti/a-C:H film annealed at 300oC.The tribologcial properties of the(Ti/)a-C:H films changed greatly after annealed,due to the graphitization,oxidation of carbon,and so forth.It compared and discussed in detail the change of the microstructure and properties of the a-C:H and Ti/a-C:H films before and after | Wang Yongxia Ye Yinping Li Hongxuan Ji Li Wang Yongjun Wu Yanxia Liu Xiaohong Chen Jianmin Zhou Huidi | 2012 | 稀有金属材料与工程2012，41，S1： | 0 |
| 16 | Effects of β-mercaptoethanol Concentrations on Developmental Ability of Oocytes in 6-week-old Dorper Sheep显示文摘This study was conducted to investigate the effects of different concentrations ofβ-mercaptoethanol on the development ability of oocytes in Dorper sheep at the age of 6 weeks.In this study,6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin,and A and B grade cumulus oocyte complexes were obtained(COCs)with different concentrations of(0,50,70 and 100μmol/L)ofβ-mercaptoethanol in the mature liquid.The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated.The results showed that compared with the cleavage rate(59.13%)and the blastocyst rate(12.17%)of the control group,the cleavage rates(60.87%,63.48%and 65.22%)and blastocyst rates(14.78%,17.39%and 21.74%)of the test groups I,II and III were higher(P>0.05),and the order was test group III>test group II>test group I>control group.The cleavage rate and blastocyst rate of the 100μmol/Lβ-mercaptoethanol group increased by 6.09 percentage points and 9.57 percentage points,respectively.It indicated that the addition of a certain amount ofβ-mercaptoethanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep.When theβ-mercaptoethanol concentration was within 100μmol/L,the cleavage rate and the blastocyst rate of oocytes were on the rise.The optimal concentration ofβ-mercaptoethanol in the mature liquid still needs further study. | Yangyi MAO Huidi LUO Zhiwu WANG Peng ZHAO Guanwu ZHANG Jun LI Hongyu GUO Li ZHANG Lina XUE Wenqing DANG | 2019 | Agricultural Biotechnology2019，8，4： | 0 |
| 17 | Role of nanoparticles in achieving macroscale superlubricity of graphene/nano-SiO_(2) particle composites显示文摘Recent studies have reported that adding nanoparticles to graphene enables macroscale superlubricity to be achieved.This study focuses on the role of nanoparticles in achieving superlubricity.First,because graphene nanoscrolls can be formed with nanoparticles as seeds under shear force,the applied load(or shear force)is adjusted to manipulate the formation of graphene nanoscrolls and to reveal the relationship between graphene-nanoscroll formation and superlubricating performance.Second,the load-carrying role of spherical nano-SiO_(2)particles during the friction process is verified by comparison with an elaborately designed fullerene that possesses a hollow-structured graphene nanoscroll.Results indicate that the incorporated nano-SiO_(2)particles have two roles in promoting the formation of graphene nanoscrolls and exhibiting load-carrying capacity to support macroscale forces for achieving macroscale superlubricity.Finally,macroscale superlubricity(friction coefficient:0.006–0.008)can be achieved under a properly tuned applied load(2.0 N)using a simple material system in which a graphene/nano-SiO_(2)particle composite coating slides against a steel counterpart ball without a decorated diamond-like carbon film.The approach described in this study could be of significance in engineering. | Panpan LI Li JI Hongxuan LI Lei CHEN Xiaohong LIU Huidi ZHOU Jianmin CHEN | 2022 | Friction2022，10，9： | 0 |
| 18 | Study on In-vitro Production Technology of Embryos from Young Dorper Sheep显示文摘This study was conducted to investigate the in-vitro production technology of embryos from young Dorper sheep, so as to provide technical support for the utilization of ovarian follicles in young Dorper sheep. Tests were conducted from the induction of Dorper sheep of 4 to 8 weeks old using follicle stimulating hormone(FSH) and pregnant horse serum(PMSG), collection of oocytes, in-vitro oocyte maturation-fertilization-zygote cultivation and 2-4-cell-stage fertilized ovum transfer. The results showed that 585 oocytes were collected from eight Dorper sheep at the age of 4 and 8 weeks, with an average of 73.13 oocytes/sheep. 346 of the 2-4-cell-stage fertilized eggs were obtained, whose cleavage rate was 59.15%. 77 in-vitro fertilized eggs at 2-4-cell stage were transplanted into 17 recipient sheep, seven of which were pregnant and gave birth to 13 'test-tube sheep' with a conception rate of 41.18%. It is indicated that the hormone induction technique, in-vitro oocyte maturation-fertilization-zygote cultivation technique and 2-4-cell-stage fertilized ovum transfer technique used in this study can serve as effective techniques for the in-vitro production of embryos from Dorper sheep of 4-8 weeks old. | Huidi LUO Yangyi MAO Huihui GUO Li ZHANG Zhiwu WANG Zhijun WANG Jun LI Hongyu GUO Lina XUE Wenqing DANG | 2019 | Agricultural Biotechnology2019，8，6： | 0 |
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