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| 1 | Tyrosine aminotransferase: biochemical and structural properties and molecular dynamics simulations显示文摘Tyrosine aminotransferase(TAT)catalyzes the transamination of tyrosine and other aromatic amino acids.The enzyme is thought to play a role in tyrosinemia type II,hepatitis and hepatic carcinoma recovery.The objective of this study is to investigate its biochemical and structural characteristics and substrate specificity in order to provide insight regarding its involvement in these diseases.Mouse TAT(mTAT)was cloned from a mouse cDNA library,and its recombinant protein was produced using Escherichia coli cells and purified using various chromatographic techniques.The recombinant mTAT is able to catalyze the transamination of tyrosine usingα-ketoglutaric acid as an amino group acceptor at neutral pH.The enzyme also can use glutamate and phenylalanine as amino group donors and p-hydroxyphenylpyruvate,phenylpyruvate and alpha-ketocaproic acid as amino group acceptors.Through macromolecular crystallography we have determined the mTAT crystal structure at 2.9Åresolution.The crystal structure revealed the interaction between the pyridoxal-5′-phosphate cofactor and the enzyme,as well as the formation of a disulphide bond.The detection of disulphide bond provides some rational explanation regarding previously observed TAT inactivation under oxidative conditions and reactivation of the inactive TAT in the presence of a reducing agent.Molecular dynamics simulations using the crystal structures of Trypanosoma cruzi TAT and human TAT provided further insight regarding the substrate-enzyme interactions and substrate specificity.The biochemical and structural properties of TAT and the binding of its cofactor and the substrate may help in elucidation of the mechanism of TAT inhibition and activation. | Prajwalini Mehere Qian Han Justin A.Lemkul Christopher J.Vavricka Howard Robinson David R.Bevan Jianyong Li | 2010 | Protein & Cell2010，1，11： | 4 |
| 2 | Omega‐3 fatty acids and/or fluvastatin in hepatitis C prior non‐responders to combination antiviral therapy – a pilot randomised clinical trial显示文摘 | David A. Sheridan Simon H. Bridge Mary M. E. Crossey Daniel J. Felmlee Fiona I. Fenwick Howard C. Thomas R. Dermot G. Neely Simon D. Taylor‐Robinson Margaret F. Bassendine | 2014 | Liver Int2014，，5： | 2 |
| 3 | The tissue effects of ultrasound--assisted lipoplasty 显示文摘 | Robinson JB Beran SJ Tan J Howard BK Zocchi ML Rohrich RJ | 1998 | Plast Reconstr Surg1998，102，1： | 1 |
| 4 | VERNAUZATION1 Modulates Root System Architecture in Wheat and Barley显示文摘 | Kai P. Voss-Fels Hannah Robinson Stephen R. Mudge Cecile Richard Saul Newman Benjamin Wittkop Andreas Stahl Wolfgang Friedt Matthias Frisch lulian Gabur Anika Miller-Cooper Bradley C. Campbell Alison Kelly Glen Fox Jack Christopher Mandy Christopher Karine Chenu Jerome Franckowiak Emma S. Mace Andrew K. Borrell Howard Eagles David R. Jordan Jose R. Botella Graeme Hammer lan D. Godwin Ben Trevaskis Rod J. Snowdon Lee T. Hickey | 2018 | Molecular Plant2018，11，1： | 1 |
| 5 | Price Fixing and Civil Damages:An Economic Analysis显示文摘 | HOWARD P MARVEL JEFFREY M NETTER ANTHONY M ROBINSON | 1988 | 40 Stanford Law Review1988，，： | 1 |
| 6 | Structure of antiviral assembly inhibitor CAP-1complex with HIV-1CA protein显示文摘 | Kelly B N Kyere S Kinde I Tang C Howard B R Robinson H Sundquist W I Summers M F Hill C P | 2007 | J Mol Biol2007，373，2： | 1 |
| 7 | Enhanced and tunable fluorescent quantum dots within a single crystal of protein显示文摘bio-nano 混血儿材料的设计和合成不能仅仅向新材料提供新奇性质,而且推进我们在 biomolecules 和他们的不能生活的对应物之间的相互作用的基本理解。这里,我们向由在溶解酵素蛋白质的单个晶体以内种 CdS 量点(QD ) 达到如此的一个目标汇报一条新途径。这个 bio-nano 混血儿比它没有水晶的对应物,和如此的荧光性质能是 Ag (I) 或 Hg (II ) 的增加分别地提高或压制的任何一个的射出许多更强壮的红荧光。在溶解酵素晶体以内的 CdS QD 的三维的加入被与电子断层摄影术扫描传播电子显微镜学揭示。更重要地,自从我们的途径没破坏溶解酵素晶体的水晶的性质,金属和蛋白质相互作用能被 X 光检查晶体学学习,因此在 CdS QD 形成提供卓见进 Cd (II ) 的角色。 | Hui Wei Stephen House Jiangjiexing Wu Jiong Zhang Zidong Wang Ying He Elizabeth J. Gao Yigui Gao Howard Robinson Wei Li Jianmin Zuo lan M. Robertson Yi Lu | 2013 | Nano Research2013，6，9： | 0 |
| 8 | Assessing the welfare of genetically altered mice显示文摘 | Wells D J Playle L C Enser W E j Flecknell P A Gardiner M A Holland j Howard B RT Hubrecht R Humphreys K R Jackson IJ Lane N Maconochie M Mason G Morton D B Raymond R Robinson V Smith J A Watt N | 2007 | 中国比较医学杂志2007，17，3： | 0 |
| 9 | Crystal structure of acetylcholinesterase catalytic subunits of the malaria vector Anopheles gambiae显示文摘 | Qian Han Dawn M. Wong Howard Robinson Haizhen Ding Polo C. H. Lam Maxim M. Totrov Paul R. Carlier Jianyong Li | 2018 | Insect Science2018，25，4： | 0 |
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