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41篇 您的检索式:作者名="Hongbin S"
    题名 作者 年代 出处 被引量
1Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research.LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER 2008Chinese Science Bulletin2008,53,7:8
2Note on therelationship between probabilistic and fuzzy clustering 显示文摘Shitong W Chung K E Hongbin S 2004Soft Computing2004,8,5:1
3Urban traffic flow prediction using a fuzzy- neural approach显示文摘Yin Hongbin Wong S C Jianmin Xu and Wong C K 2002Transportation Research Part C: Emerging Technologies2002,10,2:1
4Urban traffic flow prediction using a fuzzy-neural approach显示文摘 Wong S C Xu Jianmin 2002Transportation Research Part C2002,,10:1
5Cascaded centralized TSK fuzzy system: universal approximator and high interpretation 显示文摘Wang S Chung F L HongBin S 2005Applied Soft Computing2005,5,2:1
6Urban Traffic Flow Prediction Using a Fuzzy-neural Approach显示文摘Yin Hongbin Wong S C Xu Jianmin 2002Transportation Research Part C2002,,:1
7Mechanical characterization of short fiber reinforced phenolic foam显示文摘SHEN Hongbin NUTT S 2003Composites Part Az Applied Science and Manufacturing2003,34,9:1
8Smart 显示文摘Fangxing L Wei Q Hongbin S et transmission grid: vision and framework Transactions on SmartGrid 2010 1 (2) : al 2010IEEE2010,,:1
9Urban traffic flow prediction using a fuzzy-neural approach显示文摘Yin Hongbin Wang S C Xu Jianmin 2002Transportation Research Part C: Emerging Technologies2002,10,2:1
10Direct observation and mea surement of fiber architecture in short fiber-polymer composite foam through micro-CT imaging 显示文摘Shen Hongbin Nutt S Hull D 2004Composites Science and Technology2004,64,1314:1
11Experimental and analytical study of nonlinear bending response of sandwich beams显示文摘Sokolinsky V S Shen Hongbin Lev V Steven R N 2003Composite Structure2003,60,:1
12Mechanical characterization of short fiber reinforced phenolic foam 显示文摘Shen Hongbin Nutt S 2003Composites Part A2003,34,9:1
13Urban Traffic Flow Prediction Using a Fuzzy-neural Approach显示文摘Yin Hongbin Wang S C Xu Jianmin Wang C K 2002Transportation Research Part C:Emerging Technologies2002,10,2:1
14Note on the relationship between probabilistic and fuzzy clustering显示文摘Shitong W Chung K F Hongbin S 2004Soft Computing2004,8,5:1
15A new derivation of the APES filter显示文摘Petre S Hongbin L Jian L 1999IEEE Signal Processing Letters1999,6,8:1
16Urban traffic flow prediction using a fuzzy-neural approach显示文摘Yin Hongbin Wang S C Xu Jianmin Wang C K 2002Transportation Research Part C: Emerging Technologies2002,10,2:1
17Enhanced peel resistance of fiberreinforced phenolic foams显示文摘Shen Hongbin Lavoie A J Nutt S 2003Composites Part A:Applied Scienceand Manufacturing2003,,34:1
18Mechanical characterization of short fiberreinforced phenolic foam显示文摘Shen Hongbin Nutt S 2003Composites Part A:Applied Science andManufacturing2003,,34:1
19Urban traffic flow prediction using a fuzzy-neural approach显示文摘Yin Hongbin Wong S C Xu Jianmin 2002Transportation Research Part C2002,,10:1
20Sperm chromatin proteomics identifies evolutionarily conserved fertility factors显示文摘Chu Diana S Liu Hongbin Nix Paola 2006Nature2006,443,7107:1
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