|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 2 | Relevance of an in vitro osteoclastogenesis system to study receptor activator of NF-kB ligand and osteoprotegerin biological activities显示文摘 | Y Wittrant S Theoleyre S Couillaud C Dunstan D Heymann F Rédini | 2003 | Experimental Cell Research2003，，2： | 2 |
| 3 | Stiffness ofgeomaterials at very small strains显示文摘 | CLAYTON C R I HEYMANN G | 2001 | Geotechnique2001，51，3： | 1 |
| 4 | Global prevalence and incidence estimates of selectedcurable STDs显示文摘 | GERBASE A C ROWLEY J T HEYMANN D H | 1998 | SexTransm Dis1998，74，： | 1 |
| 5 | Validation of a hospital-laboratiry workstation for immunohematologic methods显示文摘 | Schoenfeld H Pretzel K J yon Heymann C | 2010 | Transfusion2010，50，1： | 1 |
| 6 | Patients with oral anticoagulation Bridging anticoagulation in the perioperative phase显示文摘 | Koscielny J Ziemer S von Heymann C | 2009 | Hamostaseologie2009，29，3： | 1 |
| 7 | In vitro assessment of associating osteogenetic cells with macroporous calcium-phosphate ceramics显示文摘 | Heymann D Delecrin J Deschamps C | 2001 | Rev Chit Orthop Reparatrice Appar Mot2001，87，： | 1 |
| 8 | Viral respiratory infections and asthma : the course ahead 显示文摘 | ROSENTHAL L A AVILA P C HEYMANN P W | 2010 | Allergy Clin Immunol2010，125，6： | 1 |
| 9 | Role of bone morphogenetic protein 2 in the crosstalk between endothelial progenitor cells and mesenchymal stem cells显示文摘 | Raida M Heymann AC Günther C | | 0，，04： | 1 |
| 10 | Activity of clotting factors in fresh-frozen plasma during storage at 4 degrees C over 6 days 显示文摘 | Von Heymann C Keller MK Spies C | 2009 | Transfusion2009，49，5： | 1 |
| 11 | Intermittent hirudin versus continuous heparin for antieoagulation in continuous renal replacement therapy显示文摘 | Hein OV yon Heymann C Diehl T | 2004 | Ren Fail2004，26，： | 1 |
| 12 | Role of T cells and dendritic cells in glomerular immunopathology显示文摘 | Kurts C Heymann F Lukacs-Kornek V | 2007 | Semin Immunopathol2007，29，4： | 1 |
| 13 | Intermittent hirudin versus continuous heparin for anticoagulation in continuous renal replacement therapy 显示文摘 | Hein OV von Heymann C Diehl T | 2004 | Ren Fail2004，26，3： | 1 |
| 14 | In vitro assessment of associating osteogenetic cells with macroporous calcium-phosphate ceramics显示文摘 | Heymann D Delecrin J Deschamps C | 2001 | Rev Chir Orthop Reparatrice Appar Mot2001，87，： | 1 |
| 15 | Stiffness of geometerials at very small strains显示文摘 | CLAYTON C R l HEYMANN G | 2001 | Gentechnique2001，51，3： | 1 |
| 16 | Monocytes and macrophages as cellular targets in liver fibrosis 显示文摘 | Heymann F Trautwein C Tacke F | 2009 | Inflamm Allergy Drug Targets2009，8，4： | 1 |
| 17 | Three-dimensional analysis of maxillary protraction with inter-maxillary elastics to miniplates显示文摘 | Heymann G C Cevidanes L Cornelis M | 2010 | Am J Orthod Dentofacial Orthop2010，137，20： | 1 |
| 18 | Comparative biomechanical testing of anterior and posterior stabilization procedures显示文摘 | Flamme CH Hurschler C Heymann C | 2005 | Spine2005，30，13： | 1 |
| 19 | Comparative biomechanical testing of anterior and posterior stabilization procedures 显示文摘 | Flamme CH Hurschler C Heymann C | 2005 | Spine2005，30，13： | 1 |
| 20 | Regulation of osteoclast protease expression by RANKL显示文摘 | Y Wittrant S Theoleyre S Couillaud C Dunstan D Heymann F Rédini | 2003 | Biochemical and Biophysical Research Communications2003，，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员