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| 1 | Decreases of voltage-dependent K^+ currents densities in ventricular myocytes of guinea pigs by chronic oxidant stress显示文摘AIM: To determine the changes of delayed rectifier K+ currents (Ik) and inward rectifier K+ currents (Ik1) in the ventricular myocytes of guinea pigs during the gradual apoptotic process by the chronic oxidant stress treatment. METHODS: H2O250 μmol/L (24 h) was used for inducing apoptosis in the cardiomyocytes culture of neonatal rats and to treat the isolated ventricular myocytes of adult guinea pigs in vitro for 24 h. Apoptosis was evaluated by TUNEL methods and voltage-dependent K+ currents were recorded by patch-clamp techniques. RESULTS: H2O2 50 μmol/L (24 h)induced cell apoptosis in the cardiomyocytes culture of neonatal rats. This concentration was used to treat the isolated ventricular myocytes of adult guinea pigs in vitro for 24 h and the voltage-dependent K+ currents densities (Ik, Ik1) were down-regulated. The densities of the delayed rectifier K+ currents (Ik) in 50 μmol/L H2O2 group were 2.52±0.57 pA/pF vs 5.73±1.84 pA/pF in the control group at +50 mV (n=8, P<0.01). The densities of the inward rectifier K+ currents (Ik1) in 50 μmol/L H2O2 group were -13.9± 2.70 pA/pF, 2.52±0.57 pA/pF vs -59.7±11.9 pA/pF, 5.73±1.84 pA/pF in the control group at -120 mV (n=8, P< 0.01) and -40 mV (n=8, P<0.05), respectively. The extent of inward rectifier property of Ik1 was weakened by 50 μmol/L H2O2treatment. CONCLUSION: The densities of Ik, Ik1 in the cardiomyocytes of guinea pigs were down- regulated and the inward rectifier property of Ik1 was weakened during the gradual apoptotic process after 50 μmol/L H2O2 treatment for 24 h. | De-liDONG YanLIU Yu-hongZHOU Wei-huaSONG HeWANG Bao-fengYANG | 2004 | Acta Pharmacologica Sinica2004，25，6： | 8 |
| 2 | Expression and significance of Rap1A in testes ofazoospermic subjects显示文摘Aim:To evaluate the RaplA mRNA expression and its significance in the testes of normal and azoospermic subjects.Methods:A cDNA microarray that contained RaplA and some other genes such as RBM,EIF1AY was used to identify the differential gene expression profiles between the normal and azoospermic testes,cDNA probes were prepared by labeling mRNA from azoospermic and normal testicular tissues through reverse transcription with Cy5-dUTP and Cy3-dUTP,respectively.The mixed cDNA probes were then hybridized with cDNA microarray (each containing 4096 unique human cDNA sequences).The fluorescent signals were scanned and the values of CyS-dUTP and Cy3-dUTP on each spot were analyzed and calculated.In situ hybridization was employed to detect the expres-sion of RaplA in the testes of 10 fertile and 39 azoospermic subjects.Results:One hundred and twenty-eight differentially expressed genes were found to be possibly related to azoospermia,of which 56 were up-regulated and 72,down-regulated genes.The mRNA expression of RaplA in the spermatogenic cells of azoospermic was stronger than that in those of the fertile testes.Conclusion:RaplA may play certain roles in the development of azoospermia.(Asian J Androl 2004 Mar;6:35-40) | BoYang HeWang Xiao-KangGao Bao-QiChen Yuan-QiangZhang He-LiangLiu YongWang Wei-JunQin Rong-LiangQin Guo-XingShao ChenShao | 2004 | Asian Journal of Andrology2004，6，1： | 4 |
| 3 | Upregulation of Renal Sodium Transporters in D5 Dopamine Receptor–Deficient Mice显示文摘 | Xiaoyan Wang Yingjin Luo Crisanto S. Escano Zhiwei Yang Laureano Asico Hewang Li John E. Jones Ines Armando Quansheng Lu David R. Sibley Gilbert M. Eisner Pedro A. Jose | 2010 | Hypertension2010，，6： | 1 |
| 4 | Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells显示文摘 | Li Hewang Armando Ines Yu Peiying Escano Crisanto Mueller Susette C Asico Laureano Pascua Annabelle Lu Quansheng Wang Xiaoyan Villar Van Anthony M Jones John E Wang Zheng Periasamy Ammasi Lau Yuen-Sum Soares-da-Silva Patricio Creswell Kare | 2008 | Journal of Clinical Investigation2008，，6： | 1 |
| 5 | As- sessment of water ecological carrying capacity under the two poli- cies in Tieling City on the basis of the integrated system dynam- ics model 显示文摘 | Shuo Wang Ling Xua Fenglin Yang HeWang | 2014 | Science of Total Environment2014，472，： | 1 |
| 6 | Expression and localization of mesothelin in developing rat pancreas显示文摘 | Liang‐QinHou Yin‐HeWang Li‐JieLiu JingGuo Li‐PingTeng Li‐HuaCao HuiShi LiYuan WeiDe | 2008 | Development Growth & Differentiation2008，，7： | 1 |
| 7 | A novel spectrophotometric method for quantitative determination of lactulose in food industries显示文摘 | ZhongZhang HeWang RuijinYang XiaoyanJiang | 2010 | International Journal of Food Science & Technology2010，，2： | 1 |
| 8 | Effects of low dose gamma irradiation on microbial inactivation and physicochemical properties of fried shrimp (Penaeus vannamei)显示文摘 | HeWang RuijinYang YanningLiu WeibinZhang WeiZhao YanpengZhang XiaoHua | 2010 | International Journal of Food Science & Technology2010，，6： | 1 |
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