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| 1 | Recent advances in the diagnosis and treatment of acute myocardial infarction显示文摘The Third Universal Definition of Myocardial Infarction(MI) requires cardiac myocyte necrosis with an increase and/or a decrease in a patient's plasma of cardiac troponin(cT n) with at least one cT n measurement greater than the 99 th percentile of the upper normal reference limit during:(1) symptoms of myocardialischemia;(2) new significant electrocardiogram(ECG) ST-segment/T-wave changes or left bundle branch block;(3) the development of pathological ECG Q waves;(4) new loss of viable myocardium or regional wall motion abnormality identified by an imaging procedure; or(5) identification of intracoronary thrombus by angiography or autopsy.Myocardial infarction,when diagnosed,is now classified into five types.Detection of a rise and a fall of troponin are essential to the diagnosis of acute MI.However,high sensitivity troponin assays can increase the sensitivity but decrease the specificity of MI diagnosis.The ECG remains a cornerstone in the diagnosis of MI and should be frequently repeated,especially if the initial ECG is not diagnostic of MI.There have been significant advances in adjunctive pharmacotherapy,procedural techniques and stent technology in the treatment of patients with MIs.The routine use of antiplatelet agents such as clopidogrel,prasugrel or ticagrelor,in addition to aspirin,reduces patient morbidity and mortality.Percutaneous coronary intervention(PCI) in a timely manner is the primary treatment of patients with acute ST segment elevation MI.Drug eluting coronary stents are safe and beneficial with primary coronary intervention.Treatment with direct thrombin inhibitors during PCI is non-inferior to unfractionated heparin and glycoprotein Ⅱb/Ⅲa receptor antagonists and is associated with a significant reduction in bleeding.The intra-coronary use of a glycoprotein Ⅱb/Ⅲa antagonist can reduce infarct size.Pre- and post-conditioning techniques can provide additional cardioprotection.However,the incidence and mortality due to MI continues to be high despite all these recent advances.The initial ten year experience with autologous human bone marrow mononuclear cells(BMCs) in patients with MI showed modest but significant increases in left ventricular(LV) ejection fraction,decreases in LV endsystolic volume and reductions in MI size.These studies established that the intramyocardial or intracoronary administration of stem cells is safe.However,many of these studies consisted of small numbers of patients who were not randomized to BMCs or placebo.The recent LateT ime,Time,and Swiss Multicenter Trials in patientswith MI did not demonstrate significant improvement in patient LV ejection fraction with BMCs in comparison with placebo.Possible explanations include the early use of PCI in these patients,heterogeneous BMC populations which died prematurely from patients with chronic ischemic disease,red blood cell contamination which decreases BMC renewal,and heparin which decreases BMC migration.In contrast,cardiac stem cells from the right atrial appendage and ventricular septum and apex in the SCIPIO and CADUCEUS Trials appear to reduce patient MI size and increase viable myocardium.Additional clinical studies with cardiac stem cells are in progress. | Koushik Reddy Asma Khaliq Robert J Henning | 2015 | World Journal of Cardiology2015，7，5： | 107 |
| 2 | Report of Incidence and Mortality in China Cancer Registries,2008显示文摘Objective: Annual cancer incidence and mortality in 2008 were provided by National Central Cancer Registry in China, which data were collected from population‐based cancer registries in 2011. Methods: There were 56 registries submitted their data in 2008. After checking and evaluating the data quality, total 41 registries' data were accepted and pooled for analysis. Incidence and mortality rates by area (urban or rural areas) were assessed, as well as the age‐ and sex‐specific rates, age‐standardized rates, proportions and cumulative rate. Results: The coverage population of the 41 registries was 66,138,784 with 52,158,495 in urban areas and 13,980,289 in rural areas. There were 197,833 new cancer cases and 122,136 deaths in cancer with mortality to incidence ratio of 0.62. The morphological verified rate was 69.33%, and 2.23% of cases were identified by death certificate only. The crude cancer incidence rate in all areas was 299.12/100,000 (330.16/100,000 in male and 267.56/100,000 in female) and the age‐standardized incidence rates by Chinese standard population (ASIRC) and world standard population (ASIRW) were 148.75/100,000 and 194.99/100,000, respectively. The cumulative incidence rate (0-74 years old) was of 22.27%. The crude incidence rate in urban areas was higher than that in rural areas. However, after adjusted by age, the incidence rate in urban was lower than that in rural. The crude cancer mortality was 184.67/100,000 (228.14/100,000 in male and 140.48/100,000 in female), and the age‐standardized mortality rates by Chinese standard population (ASMRC) and by world population were 84.36/100,000 and 114.32/100,000, respectively. The cumulative mortality rate (0-74 years old) was of 12.89%. Age‐adjusted mortality rates in urban areas were lower than that in rural areas. The most common cancer sites were lung, stomach, colon‐rectum, liver, esophagus, pancreas, brain, lymphoma, breast and cervix which accounted for 75% of all cancer incidence. Lung cancer was the leading cause of cancer death, followed by gastric cancer, liver cancer, esophageal cancer, colorectal cancer and pancreas cancer, which accounted for 80% of all cancer deaths. The cancer spectrum varied by areas and sex in rural areas, cancers from digestive system were more common, such as esophageal cancer, gastric cancer and liver cancer, while incidence rates of lung cancer and colorectal cancer were much higher in urban areas. In addition, breast cancer was the most common cancer in urban women followed by liver cancer, gastric cancer and colorectal cancer. Conclusion: Lung cancer, gastric cancer, colorectal cancer, liver cancer, esophageal cancer and female breast cancer contributed to the increased incidence of cancer, which should be paid more attention to in further national cancer prevention and control program. Different cancer control strategies should be carried out due to the varied cancer spectrum in different groups. | Wan-qing C hen Rong-shou Zheng Si-wei Zhang Ni Li Ping Zhao Guang-lin Li Liang-you Wu Jie He | 2012 | Chinese Journal of Cancer Research2012，24，3： | 45 |
| 3 | Liver-specific gene expression in mesenchymal stem cells is induced by liver cells显示文摘AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC. | Claudia Lange Philipp Bassler Michael V. Lioznov Helge Bruns Dietrich Kluth Axel R. Zander Henning C. Fiegel | 2005 | World Journal of Gastroenterology2005，11，29： | 31 |
| 4 | MYB34, MYB51, and MYB 122 Distinctly Regulate Indolic Glucosinolate Biosynthesis in Arabidopsis thaliana显示文摘MYB34, MYB51,和 MYB122 抄写因素被知道调整 indolic glucosinolate (IG ) 在 Arabidopsis thaliana 的生合成。决定 MYB34, MYB51,和 MYB122 的不同规章的潜力,在不同部分 ofplants 并且在有植物荷尔蒙的处理之上的 IG 的累积在 A 被分析。thaliana 幼苗。MYB34, MYB51,和 MYB122 一起行动在射击和根控制 I3M 的生合成,这被显示出,与控制主要在在 IG 的生合成的根,在射击调整生合成的 MYB51,和 MYB122 havingan 附件角色的 IG 的生合成的 MYB34。glucosinolate 的分析在 myb34 的幼苗铺平, myb51, myb122, myb34 myb51 两倍,并且面对 abscisic 酸(骆驼毛的织物) 种的 myb34 myb51 myb122 三元组大美人异种,水杨酸酸(SA ) , jasmonate (JA ) ,或乙烯(et ) 揭示了那:(1 ) MYB51 是在 SA 并且 et 发信号之上的 IG 合成的中央管理者,(2 ) MYB34 是关键 regulatorupon 骆驼毛的织物并且 JA 发信号,并且(3 ) MYB122 在 JA/ET-induced glucosinolate 生合成起仅仅一个次要的作用。myb34 myb51 myb122 三元组异种缺乏 IG,显示这三个 MYB 因素为在 standardgrowth conditions.Key 词下面的 IG 生产是不可缺少的: | Henning Frerigmann Tamara Gigolashvili | 2014 | Molecular Plant2014，7，5： | 29 |
| 5 | Serum leptin and soluble leptin receptor in non-alcoholic fatty liver disease显示文摘AIM: To determine the role of leptin system in non-al- coholic fatty liver disease (NAFLD) development by deli- neating the changes in serum levels of leptin and soluble leptin receptor (sOB-R). METHODS: Blood samples were collected from 30 consecutive patients with liver-biopsy-proven NAFLD and 30 patients with cholecystolithiasis (stationary phase) as controls. Serum leptin levels were determined by radio- immunoassay and concentration of sOB-R was measured by ELISA. Body mass index (BMI) was calculated for all subjects, and serum insulin, C-peptide, and lipoprotein levels were also detected. RESULTS: Mean serum leptin level and BMI in the NAFLD group were significantly higher than in the con- trols (both P < 0.001), but mean sOB-R level was lower in the NAFLD group when compared to the controls. Both men and women in the NAFLD group had higher mean serum leptin levels and lower sOB-R levels than did the men and women in the control group (all P < 0.001). The- re was a significant negative correlation between serum leptin and sOB-R levels (r = -0.725, P < 0.001). Multiva- riate analysis showed that the percentage of hepatocyte steatosis, sex, BMI, and homeostasis model assessment of insulin resistance (HOMA IR) were independently rela- ted to serum leptin levels. CONCLUSION: Elevated serum leptin seems to be afeature of steatosis, and serum leptin seems to increase as hepatocyte steatosis develops. An enhanced release of leptin is accompanied by an decrease in sOB-R con- centration, which suggests higher resistance of periphe- ral tissues towards the action of leptin. | Xiao-Dong Huang Yan Fan Hen Zhang Ping Wang Jing Ping Yuan Ming-Jie Li Xi-Yan Zhan | 2008 | World Journal of Gastroenterology2008，14，18： | 26 |
| 6 | Risk factors and outcome of bacterial infections in cirrhosis显示文摘Viable and non-viable pathological bacterial translocation promote a self-perpetuating circle of dysfunctional immune activation and systemic inflammation facilitating infections and organ failure in advanced cirrhosis.Bacterial infections and sepsis are now recognized as a distinct stage in the natural progression of chronic liver disease as they accelerate organ failure and contribute to the high mortality observed in decompensated cirrhosis.The increasing knowledge of structural,immunological and hemodynamic pathophysiology in advanced cirrhosis has not yet translated into significantly improved outcomes of bacterial infections over the last decades.Therefore,early identification of patients at the highest risk for developing infections and infectionrelated complications is required to tailor the currently available measures of surveillance,prophylaxis and therapy to the patients in need in order to improve the detrimental outcome of bacterial infections in cirrhosis. | Tony Bruns Henning W Zimmermann Andreas Stallmach | 2014 | World Journal of Gastroenterology2014，20，10： | 24 |
| 7 | Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells显示文摘瞄准:为了与胎儿的肝细胞(FLC ) 和可能性在合作文化调查间充质的干细胞(MSC ) 的 hepatocytic 区别膨胀,区分了 hepatocytic 房间。方法:MSC 被制动火箭与绿荧光灯的蛋白质(GFP ) 标记病毒的基因转导变异。同种细胞的显著 MSC 在用与干细胞补充的fibronectin涂的培养皿和媒介刺激条件的肝下面是也有教养的因素( SCF ), hepatocyte 生长因素( HGF ),表皮的生长因素( EGF ),和成纤维细胞生长因素 4 ( FGF-4 )独自一个,或在刚孤立的 FLC 的存在。在合作文化的房间被收获,并且 GFP+ 或 GFP- 房间用荧光被分开激活的房间排序。为肝 specific 标记 cytokeratin-18 (CK-18 ) 的反向的抄写聚合酶链反应(RT-PCR )( 法新社) ,白朊,和 alpha-fetoprotein 在不同房间人口被执行。结果:在指定文化条件下面,与 FLC co 有教养的老鼠 MSC 超过二个星期表示了白朊, CK-18,和 AFP-RNA。在 wk 3, MSC 失去了 hepatocytic 基因表示,可能由于 cocultured FLC 的增生。FLC 也在合作文化和一个很高的生长潜力显示出稳定的肝 specific 基因表情。结论:从骨髓的老鼠 MSC 能面对 FLC 在试管内区分 hepatocytic 房间,在合作文化的 MSC 的存在也为 FLC 的扩大和区别提供有益的环境。 | Claudia Lange Helge Bruns Dietrich Kluth Axel R Zander Henning C Fiegel | 2006 | World Journal of Gastroenterology2006，12，15： | 23 |
| 8 | Genetics of hepatocellular carcinoma显示文摘The completely assembled human genome has made it possible for modern medicine to step into an era rich in genetic information and high-throughput genomic analysis. These novel and readily available genetic resources and analytical tools may be the key to unravel the molecular basis of hepatocellular carcinoma (HCC). Moreover, since an efficient treatment for this disease is lacking, further understanding of the genetic background of HCC will be crucial in order to develop new therapies aimed at selected targets. We report on the current status and recent developments in HCC genetics. Special emphasis is given to the genetics and regulation of major signalling pathways involved in HCC such as p53, Wnt- signalling, TGFβ, Ras, and Rb pathways. Furthermore, we describe the influence of chromosomal aberrations as well as of DNA methylation. Finally, we report on the rapidly developing field of genomic expression profiling in HCC, mainly by microarray analysis. | Andreas Teufel Frank Staib Stephan Kanzler Arndt Weinmann Henning Schulze-Bergkamen Peter R Galle | 2007 | World Journal of Gastroenterology2007，13，16： | 21 |
| 9 | 锈革孔菌科(担子菌门,非褶菌目)中国二新记录种(英文)显示文摘1 INTRODUCTION During the investigation on wood-inhabiting fungi in China, two species of the Hymenochaetaceae were found: Fomitiporia texana (Murrill) Nuss and Inonotus ochroporus (Van der Byl) Pegler. Because they were not recorded in the Chinese fungal flora (Dai, 1999; Dai et al., 2001; Dai & Niemel?, 2006; Nú?ez & Ryvarden, 2000; Ryvarden, 2005), and their illustrated descriptions are given according to our collections. | 崔宝凯 戴玉成 Henning KNUDSEN | 2007 | 菌物学报2007，26，1： | 17 |
| 10 | Ultra-low procedural blank and the single-grain mica Rb-Sr isochron dating显示文摘Both low procedural blank and high-precision measurements of isotopic ratios are crucial for the analysis of micro samples. This study reports a chemical procedure of ultra-low blank for the high precision measurement of Sr isotopic ratios on micro samples (<1 ng level) using a new-type thermal ionization mass spectrometer IsoProbe-T, with a case study of single-grain Rb-Sr isochron dating on phlogopite from the Fuxian kimberlite in Liaoning Province. This method can be employed in studies of high resolution Rb-Sr geochronology and Sr isotopic geochemistry and thus will broaden application of the Rb-Sr isotopic system to earth sciences. | LI Qiuli HEN Fukun WANG Xiuli LI Xianghui LI Chaofeng | 2005 | Chinese Science Bulletin2005，50，24： | 15 |
| 11 | Effects of resveratrol in experimental and clinical non-alcoholic fatty liver disease显示文摘The prevalence of obesity and related conditions like non-alcoholic fatty liver disease(NAFLD) is increasing worldwide and therapeutic options are limited.Alternative treatment options are therefore intensively sought after.An interesting candidate is the natural polyphenol resveratrol(RSV) that activates adenosinmonophosphate-activated protein kinase(AMPK) and silent information regulation-2 homolog 1(SIRT1).In addition,RSV has known anti-oxidant and anti-inflammatory effects.Here,we review the current evidence for RSVmediated effects on NAFLD and address the different aspects of NAFLD and non-alcoholic steatohepatitis(NASH) pathogenesis with respect to free fatty acid(FFA) flux from adipose tissue,hepatic de novo lipogenesis,inadequate FFA β-oxidation and additional intra- and extrahepatic inflammatory and oxidant hits.We review the in vivo evidence from animal studies and clinical trials.The abundance of animal studies reports a decrease in hepatic triglyceride accumulation,liver weight and a general improvement in histological fatty liver changes,along with a reduction in circulating insulin,glucose and lipid levels.Some studies document AMPK or SIRT1 activation,and modulation of relevant markers of hepatic lipogenesis,inflammation and oxidation status.However,AMPK/SIRT1-independent actions are also likely.Clinical trials are scarce and have primarily been performed with a focus on overweight/obese participants without a focus on NAFLD/NASH and histological liver changes.Future clinical studies with appropriate design are needed to clarify the true impact of RSV treatment in NAFLD/NASH patients. | Sara Heebll Karen Louise Thomsen Steen B Pedersen Hendrik Vilstrup Jacob George Henning Grnbk | 2014 | World Journal of Hepatology2014，6，4： | 11 |
| 12 | A concept for multiterawatt fibre lasers based on coherent pulse stacking in passive cavities显示文摘Since the advent of femtosecond lasers,performance improvements have constantly impacted on existing applications and enabled novel applications.However,one performance feature bearing the potential of a quantum leap for high-field applications is still not available:the simultaneous emission of extremely high peak and average powers.Emerging applications such as laser particle acceleration require exactly this performance regime and,therefore,challenge laser technology at large.On the one hand,canonical bulk systems can provide pulse peak powers in the multi-terawatt to petawatt range,while on the other hand,advanced solid-state-laser concepts such as the thin disk,slab or fibre are well known for their high efficiency and their ability to emit high average powers in the kilowatt range with excellent beam quality.In this contribution,a compact laser system capable of simultaneously providing high peak and average powers with high wall-plug efficiency is proposed and analysed.The concept is based on the temporal coherent combination(pulse stacking)of a pulse train emitted from a high-repetition-rate femtosecond laser system in a passive enhancement cavity.Thus,the pulse energy is increased at the cost of the repetition rate while almost preserving the average power.The concept relies on a fast switching element for dumping the enhanced pulse out of the cavity.The switch constitutes the key challenge of our proposal.Addressing this challenge could,for the first time,allow the highly efficient dumping of joule-class pulses at megawatt average power levels and lead to unprecedented laser parameters. | Sven Breitkopf Tino Eidam Arno Klenke Lorenz von Grafenstein Henning Carstens Simon Holzberger Ernst Fill Thomas Schreiber Ferenc Krausz Andreas Tunnermann Ioachim Pupeza Jens Limpert | 2014 | Light(Science & Applications)2014，3，1： | 11 |
| 13 | Chemically Induced and Light-Independent Cryptochrome Photoreceptor Activation显示文摘更高的植物的 cryptochrome 光敏电阻器是二聚的蛋白质。他们的 N 终端 photosensory 域调停 dimerization,和唯一的 C 终端扩展(CCT ) 调停发信号。我们使用了与与 rapamycin 或 rapamycin 类似物(rapalogs ) 的高亲密关系绑的人的 FK506 有约束力的蛋白质(FKBP ) 。FKBPrapamycin 建筑群被另一蛋白质认出, FRB,因此允许二目标蛋白质的导致 rapamycin 的 dimerization。这里,我们由生物体之发光回声精力转移(BRET ) 表明试金到植物的这个调整 dimerization 系统的适用性。而且,我们证明由 2 明确地在 Arabidopsis 房间熔化了到 FKBP 和 FRB 和 coexpressed 的 Arabidopsis cryptochrome 的 C 终端领域组成的熔化蛋白质与 rapalog 在孵化之上在黑暗导致控制 cryptochrome 的记者和内长的基因的表示。这些结果证明 cryptochrome 信号 transduction 的激活能化学上以一种剂量依赖者方式被导致并且从轻信号解开了,并且提供地基让 gain-of-function 实验明确地在黑暗或在甚至在激活所有光敏电阻器家庭的成员的轻条件下面表明串音学习光敏电阻器的角色。 | Gesa Rosenfeldt Rafael Munoz Viana Henning D. Mootz Albrecht G. von Arnim Alfred Batschauera | 2008 | Molecular Plant2008，1，1： | 11 |
| 14 | Bariatric surgery in patients with non-alcoholic fatty liver disease-from pathophysiology to clinical effects显示文摘Non-alcoholic fatty liver disease(NAFLD) is increasingly recognized as a significant liver disease,and it covers the disease spectrum from simple steatosis with a risk of development of non-alcoholic steatohepatitis(NASH) to fibrosis,subsequent cirrhosis,end-stage liver failure,and liver cancer with a potential need for liver transplantation.NAFLD and NASH are closely related to obesity,metabolic syndrome,and type 2 diabetes(T2 D).The role of gut hormones,especially glucagon-like peptide 1(GLP-1),is important in NAFLD.Bariatric surgery has the potential for inducing great weight loss and may improve the symptoms of metabolic syndrome and T2 D.Recent data demonstrated significant effects of bariatric surgery on GLP-1 and other gut hormones and important lipid metabolic and inflammatory abnormalities in the pathophysiology of NAFLD.Therefore,bariatric surgery may reverse the pathological liver changes in NAFLD and NASH patients.In the present review,we describe NAFLD and NASH pathophysiology and the primary effects of bariatric surgery on metabolic pathways.We performed a systematic review of the beneficial and harmful effects and focused on changes in liver disease severity in NAFLD and NASH patients.The specific focus was liver histopathology as assessed by the invasive liver biopsy.Additionally,we reviewed several non-invasive methods used for the assessment of liver disease severity following bariatric surgery. | Tea L Laursen Christoffer A Hagemann Chunshan Wei Konstantin Kazankov Karen L Thomsen Filip K Knop Henning Grønbæk | 2019 | World Journal of Hepatology2019，11，2： | 11 |
| 15 | Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration显示文摘Studies have confirmed that bone marrow-derived mesenchymal stem cells(MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs(BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs(ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham(only sciatic nerve exposed), Matrigel(MG; sciatic nerve injury + intravenous transplantation of MG vehicle), ADSCs(sciatic nerve injury + intravenous MG containing ADSCs), and BMSCs(sciatic nerve injury + intravenous MG containing BMSCs) groups. Sciatic functional index was calculated to evaluate the function of injured sciatic nerve. Morphologic characteristics of nerves distal to the lesion were observed by toluidine blue staining. Spinal motor neurons labeled with Fluoro-Gold were quantitatively assessed. Compared with sham-operated rats, sciatic functional index was lower, the density of small-diameter fibers was significantly increased, and the number of motor neurons significantly decreased in rats with sciatic nerve injury. Neither ADSCs nor BMSCs significantly improved the sciatic nerve function of rats with sciatic nerve injury, increased fiber density, fiber diameters, axonal diameters, myelin sheath thickness, and G ratios(axonal diameter/fiber diameter ratios) in the sciatic nerve distal to the lesion site. There was no significant difference in the number of spinal motor neurons among ADSCs, BMSCs and MG groups. These results suggest that neither BMSCs nor ADSCs provide satisfactory results for peripheral nerve repair when using MG as the conductor for engraftment. | Marcela Fernandes Sandra Gomes Valente Rodrigo Guerra Sabongi Joao Baptista Gomes dos Santos Vilnei Mattioli Leite Henning Ulrich Arthur Andrade Nery Maria José da Silva Fernandes | 2018 | Neural Regeneration Research2018，13，1： | 10 |
| 16 | Expansive effects of aorta-gonad-mesonephros-derived stromal cells on hematopoietic stem cells from embryonic stem cells显示文摘Background Hematopoietic stem cells (HSCs) give rise to all blood and immune cells and are used in clinical transplantation protocols to treat a wide variety of refractory diseases, but the amplification of HSCs has been difficult to achieve in vitro. In the present study, the expansive effects of aorta-gonad-mesonephros (AGM) region derived stromal cells on HSCs were explored, attempting to improve the efficiency of HSC transplantation in clinical practice.Methods The murine stromal cells were isolated from the AGM region of 12 days postcoitum (dpc) murine embryos and bone marrow(BM)of 6 weeks old mice, respectively. After identification with flow cytometry and immunocytochemistry, the stromal cells were co-cultured with ESCs-derived, cytokines-induced HSCs. The maintenance and expansion of ESCs-derived HSCs were evaluated by detecting the population of CD_ 34+ and CD_ 34+Sca-1+cells with flow cytometry and the blast colony-forming cells (BL-CFCs), high proliferative potential colony-forming cells (HPP-CFCs) by using semi-solid medium colonial culture. Finally, the homing and hematopoietic reconstruction abilities of HSCs were evaluated using a murine model of HSC transplantation in vivo.Results AGM and BM-derived stromal cells were morphologically and phenotypically similar, and had the features of stromal cells. When co-cultured with AGM or BM stromal cells, more primitive progenitor cells (HPP-CFCs ) could be detected in ESCs derived hematopoietic precursor cells, but BL-CFC’s expansion could be detected only when co-cultured with AGM-derived stromal cells. The population of CD_ 34+ hematopoietic stem/progenitor cells were expanded 3 times,but no significant expansion in the population of CD_ 34+Sca-1+ cells was noted when co-cultured with BM stromal cells. While both CD_ 34+ hematopoietic stem/progenitor cells and CD_ 34+Sca-1+ cells were expanded 4 to 5 times respectively when co-cultured with AGM stromal cells. AGM region-derived stromal cells, like BM-derived stromal cells, could promote hematopoietic reconstruction and HSCs’ homing to BM in vivo. Conclusions AGM-derived stromal cells in comparison with the BM-derived stromal cells could not only support the expansion of HSCs but also maintain the self-renewal and multi-lineage differentiation more effectively. They are promising in HSC transplantation. | FU Jin-rong LIU Wen-li ZHOU Yu-feng ZHOU Jian-feng SUN Han-ying LUO Li ZHANG Hen XU Hui-zhen | 2005 | Chinese Medical Journal2005，，23： | 10 |
| 17 | Blue light is more essential than red light for maintaining the activities of photosystem Ⅱ and Ⅰ and photosynthetic electron transport capacity in cucumber leaves显示文摘Blue and red lights differently regulate leaf photosynthesis. Previous studies indicated that plants under blue light generally exhibit better photosynthetic characteristics than those under red light. However, the regulation mechanism of related photosynthesis characteristics remains largely unclear. Here, four light qualities treatments(300 μmol m–2 s–1) including white fluorescent light(FL), blue monochromatic light(B, 440 nm), red monochromatic light(R, 660 nm), and a combination of red and blue light(RB, R:B=8:1) were carried out to investigate their effects on the activity of photosystem II(PSII) and photosystem I(PSI), and photosynthetic electron transport capacity in the leaves of cucumber(Cucumis sativus L.) seedlings. The results showed that compared to the FL treatment, the R treatment significantly limited electron transport rate in PSII(ETRII) and in PSI(ETRI) by 79.4 and 66.3%, respectively, increased non-light induced non-photochemical quenching in PSII(ΦNO) and limitation of donor side in PSI(ΦND) and reduced most JIP-test parameters, suggesting that the R treatment induced suboptimal activity of photosystems and inhibited electron transport from PSII donor side up to PSI. However, these suppressions were effectively alleviated by blue light addition(RB). Compared with the R treatment, the RB treatment significantly increased ETRII and ETRI by 176.9 and 127.0%, respectively, promoted photosystems activity and enhanced linear electron transport by elevating electron transport from QA to PSI. The B treatment plants exhibited normal photosystems activity and photosynthetic electron transport capacity similar to that of the FL treatment. It was concluded that blue light is more essential than red light for normal photosynthesis by mediating photosystems activity and photosynthetic electron transport capacity. | MIAO Yan-xiu W ANG Xiao-zhuo G AO Li-hong C HEN Qing-yun QU Mei | 2016 | Journal of Integrative Agriculture2016，15，1： | 9 |
| 18 | Diagnosis and treatment of heart failure with preserved left ventricular ejection fraction显示文摘Nearly six million people in United States have heart failure.Fifty percent of these people have normal left ventricular(LV)systolic heart function but abnormal diastolic function due to increased LV myocardial stiffness.Most commonly,these patients are elderly women with hypertension,ischemic heart disease,atrial fibrillation,obesity,diabetes mellitus,renal disease,or obstructive lung disease.The annual mortality rate of these patients is 8%-12%per year.The diagnosis is based on the history,physical examination,laboratory data,echocardiography,and,when necessary,by cardiac catheterization.Patients with obesity,hypertension,atrial fibrillation,and volume overload require weight reduction,an exercise program,aggressive control of blood pressure and heart rate,and diuretics.Miniature devices inserted into patients for pulmonary artery pressure monitoring provide early warning of increased pulmonary pressure and congestion.If significant coronary heart disease is present,coronary revascularization should be considered. | Robert J Henning | 2020 | World Journal of Cardiology2020，12，1： | 9 |
| 19 | Differential changes in intrinsic innervation and interstitial cells of Cajal in small bowel atresia in newborns显示文摘AIM: To investigate morphological changes of the enteric nervous system (ENS) and the interstitial cells of Cajal (ICCs) in small bowel atresia.METHODS: Resected small bowel specimens from affected patients (n = 7) were divided into three parts (proximal, atretic, distal). Standard histology and enzyme immunohistochemistry anti-S100, anti-protein gene product (PGP) 9.5, anti-neurofilament (NF), antic-kit-receptor (CD117) was carried out on conventional paraffin sections of the proximal and distal part. RESULTS: The neuronal and glial markers (PGP 9.5, NF, S-100) were expressed in hypertrophied ganglia and nerve fibres within the myenteric and submucosal plexuses. Furthermore, the submucous plexus contained typical giant ganglia. The innervation pattern of the proximal bowel resembled intestinal neuronal dysplasia. The density of myenteric ICCs was clearly reduced in the proximal bowel, whereas a moderate number of muscular ICCs were found. The anti-CD117 immunore- action revealed additional numerous mast cells. The distal bowel demonstrated normal morphology and density of the ENS, the ICCs and the mast cells.CONCLUSION: The proximal and distal bowel in small bowel atresia revealed clear changes in morphology and density of the ENS and ICCs. | Stefan Gfroerer Roman Metzger Henning Fiegel Priya Ramachandran Udo Rolle | 2010 | World Journal of Gastroenterology2010，16，45： | 9 |
| 20 | TRAIL-induced apoptosis of hepatocellular carcinoma cells is augmented by targeted therapies显示文摘AIM:To analyze the effect of chemotherapeutic drugs and specific kinase inhibitors,in combination with the death receptor ligand tumor necrosis factor-related apoptosis inducing ligand(TRAIL),on overcoming TRAIL resistance in hepatocellular carcinoma(HCC)and to study the efficacy of agonistic TRAIL antibodies,as well as the commitment of antiapoptotic BCL-2 proteins, in TRAIL-induced apoptosis. METHODS:Surface expression of TRAIL receptors (TRAIL-R1-4)and expression levels of the antiapoptotic BCL-2 proteins MCL-1 and BCL-xL were analyzed by flow cytometry and Western blotting,respectively. Knock-down of MCL-1 and BCL-xL was performed by transfecting specific small interfering RNAs.HCC cellswere treated with kinase inhibitors and chemotherapeutic drugs.Apoptosis induction and cell viability were analyzed via flow cytometry and 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. RESULTS:TRAIL-R1 and-R2 were profoundly expressed on the HCC cell lines Huh7 and Hep-G2. However,treatment of Huh7 and Hep-G2 with TRAIL and agonistic antibodies only induced minor apoptosis rates.Apoptosis resistance towards TRAIL could be considerably reduced by adding the chemotherapeutic drugs 5-fluorouracil and doxorubicin as well as the kinase inhibitors LY294002[inhibition of phosphoinositol- 3-kinase(PI3K)],AG1478(epidermal growth factor receptor kinase),PD98059(MEK1),rapamycin(mam- malian target of rapamycin)and the multi-kinase inhibitor Sorafenib.Furthermore,the antiapoptotic BCL-2 proteins MCL-1 and BCL-xL play a major role in TRAIL resistance:knock-down by RNA interference increased TRAIL-induced apoptosis of HCC cells.Additionally, knock-down of MCL-1 and BCL-xL led to a significant sensitization of HCC cells towards inhibition of both c-Jun N-terminal kinase and PI3K.CONCLUSION:Our data identify the blockage of survival kinases,combination with chemotherapeutic drugs and targeting of antiapoptotic BCL-2 proteins as promising ways to overcome TRAIL resistance in HCC. | Bruno Christian Koehler Toni Urbanik Binje Vick Regina Johanna Boger Steffen Heeger Peter R Galle Marcus Schuchmann Henning Schulze-Bergkamen | 2009 | World Journal of Gastroenterology2009，15，47： | 9 |
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