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| 1 | Cardioprotection of sevoflurane postconditioning by activating extracellular signal-regulated kinase 1/2 in isolated rat hearts^1显示文摘瞄准:1/2 免于的细胞外的调整信号的 kinase (英皇家空军之阶级最低之兵) 的激活是化学家灌注损害。ERK1/2 是否调停 sevoflurane 柱子调节的 cardioprotection 是未知的。我们测试了 sevoflurane 柱子调节是否经由 ERK1/2-depen-dent 机制生产 cardioprotection。方法:在协议 1 , 酒Langendorff 的 Sprague Dawley 老鼠心( n=84 , 12 每组)与假冒的组的异常,受到 90 min 灌注跟随的 30 min 局部缺血并且被分到未经治疗(控制)组,由 4 个周期列在后面是化学家帖子调节(各个的 25 s ),3%( v/v ) sevoflurane 帖子调节(为 5 min 和冲刷的 10 min ),并且 PD98059 溶剂 DMSO (<0.2%), ERK1/2 禁止者 PD98059 ( 20 μmol/L ),和 Sevo+PD 管理。在平衡的 30 min 的左室的 hemodynamics 和冠的流动分别地在灌注的 30, 60,和 90 min 被记录。尖锐梗塞尺寸被 triphenyltetrazolium 氯化物染色测量。线粒体的配置被一台电子显微镜观察。西方的污点分析被用来在灌注的结束决定 cytosolic 和 mitochondrial 细胞色素 c 的内容。在协议 2,在灌注的 15 min 以后,总数的表示和 ERK1/2 和它的下游的目标 p70S6K 的 phosphorylated 形式由西方的弄污是坚定的。结果:在基线 hemodynamics 的差别都没在试验性的组(P>0.05 ) 之中被观察。在灌注以后,与控制组相比, sevoflurane 柱子调节并且显著地是化学家柱子调节(P<0.05 ) 改进了功能的恢复并且(P<0.05 ) 大部分减少了心肌的梗塞尺寸(22.9%± 4 .6% 和 21.2%± 3 .8% ,对 39.4%± 5 .7% ,两 P<0.05 ) 。调停 Sevoflurane 的保护被 PD98059 废除。结论:由 sevoflurane 的麻药柱子调节有效地由在 vitro 激活 ERK1/2 免于灌注损坏。 | Hong-tao CHEN Cheng-xiang YANG Heng LI Cheng-jing ZHANG Xian-jie WEN Jun ZHOU You-ling Fan Teng HUANG Yin-ming ZENG | 2008 | Acta Pharmacologica Sinica2008，29，8： | 41 |
| 2 | Sinomenine induces apoptosis in RAW 264.7 cell- derived osteoclasts in vitro via caspase-3 activation显示文摘 | Long-gang HE Xiang-lian LI Xiang-zhou ZENG Heng DUAN Song WANG Lin-sheng LEI Xiao-juan LI Shu-wen LIU | 2014 | Acta Pharmacologica Sinica2014，35，2： | 10 |
| 3 | Intrathecal administration of Ca_v3.2 and Ca_v3.3 antisense oligonucleotide reverses tactile allodynia and thermal hyperalgesia in rats following chronic compression of dorsal root of ganglion显示文摘瞄准:现在的学习试图阐明在在针的 level.METHODS 的神经病的疼痛的致病的 T 子类型钙隧道(Cav3.1, Cav3.2,和 Cav3.3 ) 的角色:背面的根中心(电荷耦合器件) 的长期的压缩老鼠模型被收养。Cav3.1, Cav3.2,和 Cav3.3 或正常的 Theantisense oligonucleotide 盐(NS ) 每从第一天的天是 intrathecallyadministered 两次到在操作以后的第四天。爪机械退却阀值和爪热退却潜伏被测量分别地评估 tactileallodynia 和热痛觉过敏。结果:电荷耦合器件老鼠在操作以后开发了可靠 tactileallodynia 和热痛觉过敏。Cav3.2 和 Cav3.3 的 antisenseoligonucleotide 的 Intrathecal 管理显著地在电荷耦合器件老鼠,然而并非 Cav3.1 减轻了触觉的 allodynia 和热痛觉过敏。结论:在针的绳索的 Cav3.2 和 Cav3.3 子类型钙隧道可以在神经病的疼痛的致病起一个重要作用,它可以贡献神经病的疼痛的管理。 | Xian-jie WEN Zhang-jun LI Zhi-xin CHEN Zhi-yuan FANG Chen-xiang YANG Heng LI Yin-ming ZENG | 2006 | Acta Pharmacologica Sinica2006，27，12： | 8 |
| 4 | Beneficial effects of a diabetes specific formula on insulin sensitivity and free fatty acid in patients with type 2 diabetes mellitus显示文摘背景这未来的、使随机化的、控制研究被设计调查糖尿病的效果特定的公式(Diason 低 energy:313.8 kJ/100 ml ) 与一个标准公式相比,在在类型 2 糖尿病患者的胰岛素敏感,浆液 C 肽,浆液类脂化合物和免费的丰满的酸(船边交货) 上。2 名糖尿病患者完成了学习的总共 71 类型的方法。Enteral 公式作为营养的唯一的来源口头上地被给这些题目 6 天。静脉的血样品(0.5,1,2,3 个小时) 在 75 g 以后在 day-7 被收集口头的葡萄糖忍耐测试(OGTT ) ,在标准测试饭(1673.6 kJ ) 以后并且在 6 天也以后的白天 1 测试糖尿病特定的公式或一个标准公式。血浆葡萄糖,浆液胰岛素, C 肽和类脂化合物是。测量。在干预时期以后结果,糖尿病特定的公式在 0.5 小时( P0.05 )和 1 个小时( P0.01 )在血葡萄糖集中导致了显著地更低的饭后的上升;血浆葡萄糖( P=0.05 )的显著地更低的山峰高度;在 0.5 小时( P0.01 )的显著地更低的血浆胰岛素集中, 1 个小时( P0.01 )和 2 个小时( P0.01 );并且与控制相比的一座显著地更低的血浆胰岛素山峰;OGTT 和标准测试饭(P0.05 ) 在在糖尿病以后的曲线下面的 .The 葡萄糖和胰岛素区域与标准公式相比的特定的公式显著地更低。C 肽水平在 6 天公式比作 75 g OGTT 的两营养以后是更低的,然而并非与标准混合的饭不同。两个公式是井 toleraled。在摘要的结论糖尿病有相对高的 monounsaturated 的特定的公式丰满的酸和高度多,纤维比例显著地改进了 glycemic 控制。在这上,胰岛素敏感(HOMA ) 显著地被改进并且可以直接因此在长期的复杂并发症上改进影响。疾病特定的公式应该因此是被糖尿病患者和 hyperglycemic 病人需要营养的支持要过去常的比较喜欢的选择。 | LI Yu-xiu ZENG Jing-bo YU Kang SUN Qi LIU Qiu-ying QIN Wei ZHANG Qian YU Jian-chun WANG Heng | 2008 | Chinese Medical Journal2008，，8： | 8 |
| 5 | Proteomic analysis identifies translationally controlled tumor protein as a mediator of phosphatase of regenerating liver-3-promoted proliferation, migration and invasion in human colon cancer cells显示文摘背景可观的证据建议改革 liver-3 (PRL-3 ) 的那磷酸酶在癌症转移起多重作用;然而,分子的机制大部分留下这研究的 unknown.The 目的是识别与 PRL-3-promoted 结肠癌联系的蛋白质转移,由有 PRL-3 基因( LoVo-PRL-3 )或空向量的人的结肠癌 LoVo 房间 transfected 的比较 proteomic analysis.Methods Proteomes , PAcGFP-C3 (LoVo控制)用在集中显著地变化了的 2D 胶化 electrophoresis.Proteins 被比较被选择并且鉴别了使用集体 spectrometry.Expression | CHU Zhong-hua LIU Lu ZHENG Chao-xu LAI Wei LI Shou-feng WU Heng ZENG Yu-jie ZHAO Hai-yan GUAN Yu-feng | 2011 | Chinese Medical Journal2011，，22： | 6 |
| 6 | Solute-homogenization model and its experimental verification in Mg-Gd-based alloys显示文摘Composition homogenization in solid solution is important for industrial alloys. In the present work, a solute homogenization model is proposed based on the chemical short-range-order tendency in Mg-Gdbased alloys. After a calculation using the cluster-plus-glue-atom model, the stable Mg-Gd structural unit is derived, [Gd-Mg12]Mg6, where one solute Gd is nearest-neighbored with twelve Mg atoms to form the characteristic hcp cluster [Gd-Mg12] and this cluster is matched with six Mg glue atoms. Such a local unit is then mixed with [Mg-Mg12]Mg3, the stable unit for pure Mg. Assuming that the Gd-containing units are arranged in fcc-or bcc-like lattice points and the Mg units in their octahedral interstices, three proportions between the two units are obtained, 1:1, 2:3, and 1:3, which constitute three solute homogenization modes. The prevailing Mg-Gd-based alloys are consequently classified into three groups, respectively exemplified by GW103 K(Mg-10 Gd-3 Y-0.4 Zr, wt%), GW83 K(Mg-8 Gd-3 Y-0.4 Zr), and GW63 K(Mg-6 Gd-3 Y-0.4 Zr). Mg-Gd-Y-Zr alloys were designed following the model(where Y and Zr were also added in substitution for Gd) and prepared by permanent-mould casting. According to their mechanical properties,the 1:3 alloy(Mg-5.9 Gd-1.6 Y-0.4 Zr) shows the best comprehensive properties(ultimate tensile strength305 MPa, yield strength 186 MPa, elongation 9.0%) in solution plus ageing state. | Shengnan Qian Chuang Dong Tianyu Liu Ying Qin Qing Wang Yujuan Wu Lidong Gu Jianxin Zou Xiangwen Heng Liming Peng Xiaoqin Zeng | 2018 | Journal of Materials Science & Technology2018，34，7： | 6 |
| 7 | Age and menopausal status are important factors influencing the serum human epididymis secretory protein 4 level:a prospective cross-sectional study in healthy Chinese people显示文摘Background::Human epididymis secretory protein 4(HE4)is a new ovarian cancer biomarker.The factors influencing HE4 levels are not clear,and the reference data in China are limited.Here,we aim to evaluate the effects of menopause and age on HE4 levels and to provide a possible reference value for HE4 in healthy Chinese people.Methods::A total of 2493 healthy females aged 40 years or older were recruited from March 2013 to March 2017 with the cooperation of four medical institutions across Beijing,China.The serum levels of HE4 and cancer antigen 125(CA125)were measured by enzyme-linked immunosorbent assay.The Wilcoxon rank-sum test of variance and a stratified analysis were used to analyze the relationships among age,menopausal status,and levels of HE4 or CA125.Confidence intervals(5%-95%)were determined for reference ranges in different populations.Results::There was a statistically significant difference in median HE4 levels between the post-menopausal(n=2168)and premenopausal groups(n=325)(36.46 vs.24.04 pmol/L,Z=-14.41,P<0.001).HE4 increased significantly with age in the post-menopausal groups(H=408.18,P<0.001)but not in the pre-menopausal subjects(Z=-0.43,P=0.67).The upper 95th percentile of HE4 levels were 44.63 pmol/L for pre-menopausal women,78.17 pmol/L for post-menopausal women,and 73.3 pmol/L for all women.In the post-menopausal population,the HE4 reference ranges were 13.15 to 47.31,14.31 to 58.04,17.06 to 73.51,24.50 to 115.25,and 35.71 to 212.37 pmol/L for different age groups from forty divided by decade.The CA125 level was affected mainly by menopausal status and not age.Conclusions::Menopausal status and age were both important factors influencing the level of HE4,and age affected HE4 levels mainly in post-menopausal women.The HE4 level was higher in the post-menopausal population than in the pre-menopausal population and increased with age. | Hong-Yan Cheng Lin Zeng Xue Ye Rui-Qiong M a Zhi-Jian Tang Hong-Ling Chu Yi-M ing Zhao Li-Rong Zhu Yu-Nong Gao Xiao-Hong Chang Heng Cui | 2020 | Chinese Medical Journal2020，，11： | 6 |
| 8 | Spatio-temporal variation in rock exhumation linked to large-scale shear zones in the southeastern Tibetan Plateau显示文摘Crustal-scale shear zones are believed to have played an important role in the tectonic and landscape evolution of orogens. However, the variation of long-term rock exhumation between the interior of shear zones and adjacent regions has not been documented in detail. In this study, we obtained new zircon U-Pb, biotite ^(40)Ar/^(39)Ar, zircon and apatite(U-Th)/He data, and conducted inverse thermal history modeling from two age-elevation profiles(the Pianma and Tu'er profiles) in the southeastern Tibetan Plateau. Our goal is to constrain the exhumation history of the Gaoligong and Chongshan shear zones and adjacent regions, so as to explore the effect of the shear zones on exhumation and their thermal effect on cooling that should not be ignored. Our results suggest that during the interval of 18–11 Ma the exhumation rates of rocks within the Gaoligong shear zone are anomalously high compared with those outside of. The rapid cooling during 18–11 Ma appears to be restricted to the shear zone, likely due to localized thermal effects of shearing and exhumation. After 11 Ma, both the areas within and outside of the shear zones experienced a similar two-stage exhumation history: slower cooling until the early Pliocene, and then a rapid increase in cooling rate since the early Pliocene. Our results indicate a synchronized exhumation but with spatially varied exhumation rates. Our study also highlights the important role of large-scale shear zones in exposing rocks, and thus the importance of the structural context when interpreting thermochronological data in the southeastern margin of the Tibetan Plateau. | Yukui GE Jing LIU-ZENG Jinyu ZHANG Wei WANG Yuntao TIAN Matthew FOX Lingsen ZENG Xiaoming SHEN Heng WANG Ze SU Kejia XIE | 2020 | Science China Earth Sciences2020，63，4： | 5 |
| 9 | A new way to synthesize ZnS nanoparticles显示文摘ZnS nanoparticles were prepared with normal ZnO and Na2S by solid-liquid chemical reaction under ultrasonic condition andcharacterized by XRD,TEM,SEM,IR and TG-DTG.The results showed that these particles were good crystal cubic zinc blendedwith average size of 50 nm,possess good IR transmittance in the range of 400-4000 cm and good thermal stability. | Heng Zhi Zeng Ke Qiang Qiu Yuan Yuan Du Wen Zhang Li | 2007 | Chinese Chemical Letters2007，18，4： | 5 |
| 10 | Dissecting human embryonic skeletal stem cell ontogeny by single-cell transcriptomic and functional analyses显示文摘Human skeletal stem cells(SSCs)have been discovered in fetal and adult long bones.However,the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive.Here we map the transcriptional landscape of human limb buds and embryonic long bones at single-cell resolution to address this fundamental question.We found remarkable heterogeneity within human limb bud mesenchyme and epithelium,and aligned them along the proximal-distal and anterior-posterior axes using known marker genes.Osteo-chondrogenic progenitors first appeared in the core limb bud mesenchyme,which give rise to multiple populations of stem/progenitor cells in embryonic long bones undergoing endochondral ossification.Importantly,a perichondrial embryonic skeletal stem/progenitor cell(eSSPC)subset was identified,which could self-renew and generate the osteochondral lineage cells,but not adipocytes or hematopoietic stroma.eSSPCs are marked by the adhesion molecule CADM1 and highly enriched with FOXP1/2 transcriptional network.Interestingly,neural crest-derived cells with similar phenotypic markers and transcriptional networks were also found in the sagittal suture of human embryonic calvaria.Taken together,this study revealed the cellular heterogeneity and lineage hierarchy during human embryonic skeletogenesis,and identified distinct skeletal stem/progenitor cells that orchestrate endochondral and intramembranous ossification. | Jian He Jing Yan Jianfang Wang Liangyu Zhao Qian Xin Yang Zeng Yuxi Sun Han Zhang Zhijie Bai Zongcheng Li Yanli Ni Yandong Gong Yunqiao Li Han He Zhilei Bian Yu Lan Chunyu Ma Lihong Bian Heng Zhu Bing Liu Rui Yue | 2021 | Cell Research2021，31，7： | 5 |
| 11 | Angiopoletin-1 protects myocardial endothelial cell function blunted by angiopoietin-2 and high glucose condition显示文摘 | Qin-hui TUO Guo-zuo XIONG Heng ZENG Hei-di YU Shao-wei SUN Hong-yan LING Bing-yang ZHU Duan-fang LIAO Jian-xiong CHEN | 2011 | Acta Pharmacologica Sinica2011，32，1： | 4 |
| 12 | A new approach to the facile synthesis of 2-substituted-quinazolin-4(3H)-ones显示文摘2-substituted-quinazolin-4 (3H ) 的灵巧的合成的一条新途径用代替的 2-aminobenzamide 和 orthoesters 的冷凝作用反应的和它的衍生物被报导。 | Bin Wang Zeng Li Xiao Ning Wang Jia Heng Tan Lian Quan Gu Zhi Shu Huang | 2011 | Chinese Chemical Letters2011，22，8： | 4 |
| 13 | Electrochemical properties of carbon-coated LiFePO_4 and LiFe_(0.98)Mn_(0.02)PO_4 cathode materials synthesized by solid-state reaction显示文摘Olivine structured LiFePO 4 /C (lithium iron phosphate) and Mn 2+ -doped LiFe 0.98 Mn 0.02 PO 4 /C powders were synthesized by the solid-state reaction. The effects of manganese partial substitution and different carbon content coating on the surface of LiFePO 4 were considered. The structures and electrochemical properties of the samples were measured by X-ray diffraction (XRD), cyclic voltammetry (CV), charge/discharge tests at different current densities, and electrochemical impedance spectroscopy (EIS). The electrochemical properties of LiFePO 4 cathodes with x wt.% carbon coating (x= 3, 7, 11, 15) at =0.2C, 2C (1C= 170 mAh·g 1 ) between 2.5 and 4.3 V were investigated. The measured results mean that the LiFePO 4 with 7 wt.% carbon coating shows the best rate performance. The discharge capacity of LiFe 0.98 Mn 0.02 PO 4 /C composite is found to be 165 mAh·g 1 at a discharge rate, = 0.2C, and 105 mAh·g 1 at =2C, respectively. After 10 cycles, the discharge capacity has rarely fallen, while that of the pristine LiFePO 4 /C cathode is 150 mAh·g 1 and 98 mAh·g 1 at =0.2 and 2C, respectively. Compared to the discharge capacities of both electrodes above, the evident improvement of the electrochemical performance is observed, which is ascribed to the enhancement of the electronic conductivity and diffusion kinetics by carbon coating and Mn 2+-substitution. | Lin, Ying Zeng, Baozhi Lin, Yingbin Li, Xiaowei Zhao, Guiying Zhou, Ting Lai, Heng Huang, Zhigao | 2012 | Rare Metals2012，31，2： | 3 |
| 14 | The membrane-associated E3 ubiquitin ligase MARCH3 downregulates the IL-6 receptor and suppresses colitis-associated carcinogenesis显示文摘The IL-6-STAT3 axis is critically involved in inflammation-associated carcinogenesis(IAC).How this axis is regulated to modulate IAC remains unknown.Here,we show that the plasma membrane-associated E3 ubiquitin ligase MARCH3 negatively regulates STAT3 activation triggered by IL-6,as well as another IL-6 subfamily member,Oncostatin M(OSM).MARCH3 is associated with the IL-6 receptorα-chain(IL-6Rα)and its coreceptor gp130.Biochemical experiments indicated that MARCH3 mediates the polyubiquitination of IL-6Rαat K401 and gp130 at K849 following IL-6 stimulation,leading to their translocation to and degradation in lysosomes.MARCH3 deficiency increases IL-6-and OSM-triggered activation of STAT3 and induction of downstream effector genes in various cell types.MARCH3 deficiency enhances dextran sulfate sodium(DSS)-induced STAT3 activation,increases the expression of inflammatory cytokines,and exacerbates colitis,as well as azoxymethane(AOM)/DSS-induced colitis-associated cancer in mice.In addition,MARCH3 is downregulated in human colorectal cancer tissues and associated with poor survival across different cancer types.Our findings suggest that MARCH3 is a pivotal negative regulator of IL-6-induced STAT3 activation,inflammation,and inflammation-associated carcinogenesis. | Heng Lin Lu Feng Kai-Sa Cui Lin-Wen Zeng Deng Gao Long-Xiang Zhang Wen-Hua Xu Yu-Hao Sun Hong-Bing Shu Shu Li | 2021 | Cellular & Molecular Immunology2021，18，12： | 3 |
| 15 | Association of Polygenic Risk Score with Age at Onset and Cerebrospinal Fluid Biomarkers of Alzheimer’s Disease in a Chinese Cohort显示文摘To evaluate whether the polygenic profile modifies the development of sporadic Alzheimer’s disease(sAD)and pathological biomarkers in cerebrospinal fluid(CSF),462 sAD patients and 463 age-matched cognitively normal(CN)controls were genotyped for 35 singlenucleotide polymorphisms(SNPs)that are significantly associated with sAD.Then,the alleles found to be associated with sAD were used to build polygenic risk score(PRS)models to represent the genetic risk.Receiver operating characteristic(ROC)analyses and the Cox proportional hazards model were used to evaluate the predictive value of PRS for the sAD risk and age at onset.We measured the CSF levels of Aβ42,Aβ42/Aβ40,total tau(T-tau),and phosphorylated tau(P-tau)in a subgroup(60 sAD and 200 CN participants),and analyzed their relationships with the PRSs.We found that 14 SNPs,including SNPs in the APOE,BIN1,CD33,EPHA1,SORL1,and TOMM40 genes,were associated with sAD risk in our cohort.The PRS models built with these SNPs showed potential for discriminating sAD patients from CN controls,and were able to predict the incidence rate of sAD and age at onset.Furthermore,the PRSs were correlated with the CSF levels of Aβ42,Aβ42/Aβ40,T-tau,and P-tau.Our study suggests that PRS models hold promise for assessing the genetic risk and development of AD.As genetic risk profiles vary among populations,large-scale genome-wide sequencing studies are urgently needed to identify the genetic risk loci of sAD in Chinese populations to build accurate PRS models for clinical practice. | Wei-Wei Li Zhen Wang Dong-Yu Fan Ying-Ying Shen Dong-Wan Chen Hui-Yun Li Ling Li Heng Yang Yu-Hui Liu Xian-Le Bu Wang-Sheng Jin Fan Zeng Zhi-Qiang Xu Jin-Tai Yu Li-Yong Chen Yan-Jiang Wang | 2020 | Neuroscience Bulletin2020，36，7： | 3 |
| 16 | Traffic emission and its impact on air quality in Guangzhou area显示文摘A comprehensive field measurement was set up in Guangzhou City of China and the key was placed on NO\-x and O\-3 pollution. The results indicated that the average driving speed of vehicle was only 14 km/h in downtown with high frequency of idle and acceleration. Upward fluxes of CO and NO were observed in Dongfeng Street. NO\-x annual mean concentration in urban area increased year by year, and NO\-x was identified as the most important pollutant since 1995. Photochemical smog pollution was serious in general, spatial and seasonal distribution of O\-3 was observed. O\-3 concentration was kept in a high level in autumn, and its formation was restrained in summer due to frequent thunderstorm and high humidity. The numerical simulation showed the average concentration and maximum concentration of O\-3 would increase 60%—100% if vehicular emission increased 100% in Guangzhou. | Zhang Yuan\|hang, Xie Shao\|dong, Zeng Li\|min, Wang Hui\|xiang Laboratory of Atmospheric Environmental Simulation, ESPC, Center of Environmental Sciences, Peking University, Beijing 100871, China Yu Kai\|heng, Zhu Chang\|jian, Pan Nan\|ming, Wang | 1999 | Journal of Environmental Sciences1999，11，3： | 3 |
| 17 | Multi-omics approaches identify SF3B3 and SIRT3 as candidate autophagic regulators and druggable targets in invasive breast carcinoma显示文摘Autophagy is a critical cellular homeostatic mechanism,and its dysfunction is linked to invasive breast carcinoma(BRCA).Recently,several omics methods have been applied to explore autophagic regulators in BRCA;however,more reliable and robust approaches for identifying crucial regulators and druggable targets remain to be discovered.Thus,we report here the results of multi-omics approaches to identify potential autophagic regulators in BRCA,including gene expression(EXP),DNA methylation(MET)and copy number alterations(CNAs)from The Cancer Genome Atlas(TCGA).Newly identified candidate genes,such as SF3 B3,TRAPPC10,SIRT3,MTERFD1,and FBXO5,were confirmed to be involved in the positive or negative regulation of autophagy in BRCA.SF3 B3 was identified firstly as a negative autophagic regulator,and siRNA/shRNA-SF3 B3 were shown to induce autophagyassociated cell death in in vitro and in vivo breast cancer models.Moreover,a novel small-molecule activator of SIRT3,1-methylbenzylamino amiodarone,was discovered to induce autophagy in vitro and in vivo.Together,these results provide multi-omics approaches to identify some key candidate autophagic regulators,such as the negative regulator SF3 B3 and positive regulator SIRT3 in BRCA,and highlight SF3 B3 and SIRT3 as new druggable targets that could be used to fill the gap between autophagy and cancer drug development. | Shouyue Zhang Jin Zhang Yang An Xiaoxi Zeng Ziyi Qin Yuqian Zhao Heng Xu Bo Liu | 2021 | Acta Pharmaceutica Sinica B2021，11，5： | 3 |
| 18 | Effects of NS1 variants of H5N1 influenza virus on interferon induction, TNFa response and p53 activity显示文摘Non-structural protein 1(NS1)is an important virulence factor of the highly pathogenic H5N1 avian influenza virus.A five-amino-acid(5 aa)deletion at position 80–84 and an aspartic acid to glutamic acid substitution at position 92(D92E)are two major NS1 mutations that are highly correlated with enhanced virulence.To investigate the effect of these mutations in H5N1 virulence,three H5N1-NS1 variants were constructed:NS51(lacking 5 aa at position 80–84),NS51(I)(carrying a 5-aa insertion at position 80–84)and NS51(IM)(carrying both the 5-aa insertion and the D92E mutation).We examined the effects of these mutations on interferon(IFN)induction,tumor-necrosis factor(TNF)a response,p53 activity and apoptosis.We found that the D92E mutation eliminated NS1’s repressive effect on IFN induction,while the 5-aa deletion resulted in enhanced resistance to TNFa responses.We also observed that all three variants exhibited a similar suppressive effect on p53 transcriptional activity,although none of them significantly influenced apoptosis of host cells.Our findings shed new light on the role of NS1 in the pathogenicity of H5N1 virus. | Weizhong Li Gefei Wang Heng Zhang Gang Xin Dangui Zhang Jun Zeng Xiaoxuan Chen Yanxuan Xu Youhong Cui Kangsheng Li | 2010 | Cellular & Molecular Immunology2010，7，3： | 3 |
| 19 | An enrichment model using regular health examination data for early detection of colorectal cancer显示文摘Objective: Challenges remain in current practices of colorectal cancer(CRC) screening, such as low compliance,low specificities and expensive cost. This study aimed to identify high-risk groups for CRC from the general population using regular health examination data.Methods: The study population consist of more than 7,000 CRC cases and more than 140,000 controls. Using regular health examination data, a model detecting CRC cases was derived by the classification and regression trees(CART) algorithm. Receiver operating characteristic(ROC) curve was applied to evaluate the performance of models. The robustness and generalization of the CART model were validated by independent datasets. In addition, the effectiveness of CART-based screening was compared with stool-based screening.Results: After data quality control, 4,647 CRC cases and 133,898 controls free of colorectal neoplasms were used for downstream analysis. The final CART model based on four biomarkers(age, albumin, hematocrit and percent lymphocytes) was constructed. In the test set, the area under ROC curve(AUC) of the CART model was 0.88 [95%confidence interval(95% CI), 0.87-0.90] for detecting CRC. At the cutoff yielding 99.0% specificity, this model’s sensitivity was 62.2%(95% CI, 58.1%-66.2%), thereby achieving a 63-fold enrichment of CRC cases. We validated the robustness of the method across subsets of test set with diverse CRC incidences, aging rates, genders ratio, distributions of tumor stages and locations, and data sources. Importantly, CART-based screening had the higher positive predictive value(1.6%) than fecal immunochemical test(0.3%).Conclusions: As an alternative approach for the early detection of CRC, this study provides a low-cost method using regular health examination data to identify high-risk individuals for CRC for further examinations. The approach can promote early detection of CRC especially in developing countries such as China, where annual health examination is popular but regular CRC-specific screening is rare. | Qiang Shi Zhaoya Gao Pengze Wu Fanxiu Heng Fuming Lei Yanzhao Wang Qingkun Gao Qingmin Zeng Pengfei Niu Cheng Li Jin Gu | 2019 | Chinese Journal of Cancer Research2019，31，4： | 3 |
| 20 | Prediction of chemotherapeutic resistance in serous ovarian cancer with low-density custom microarray显示文摘To the Editor:Ovarian cancer is the leading cause of death from gynecologic malignancies.The major limitation to the successful long-term treatment of ovarian cancer is the development of chemotherapeutic resistance,which involves thousands of genes.In this study,a single-channel low-density custom microarray was designed to compare gene expression profiles between chemotherapy-resistant and chemotherapy-sensitive ovarian cancer.Tfie 87 genes were identified to be differentially expressed in resistant and sensitive specimens that can be used to fairly accurately predict drug resistance in serous ovarian cancer. | Hong-Lan Zhu Hao-Xia Zeng Xu-Dong Liang Xiao-Hong Chang Jian-Liu Wang Heng Cui Li-Hui Wei | 2020 | Chinese Medical Journal2020，，7： | 2 |
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