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| 1 | Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale(Brassica oleracea var.alboglabra Bailey)显示文摘ζ-Carotene desaturase(ZDS)is an important enzyme in carotenoid biosynthesis.Here,the Brassica oleracea var.alboglabra ZDS(Boa ZDS)gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction(RT-PCR)and deposited in Gen Bank(accession number KY662297).The Boa ZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein.Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B.oleracea var.capitata and B.rapa.The promoter sequence of the Boa ZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses.Semiquantitative RT-PCR analysis showed that Boa ZDS expression varied among different developmental stages and organs.Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage.The leaves showed the highest ZDS expression levels compared to the other organs.ZDS expression decreased in all flower tissues during blooming.The fused protein of Boa ZDS was obtained by prokaryotic expression.Heterologous expression of Boa ZDS in Escherichia coli confirmed that Boa ZDS encodes a functionalζ-carotene desaturase that increases β-carotene accumulation in E.coli cells harboring a β-carotene-producing plasmid.The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. | SUN Bo ZHANG Fen XUE Shengling CHANG Jiaqi ZHENG Aihong JIANG Min MIAO Huiying WANG Qiaomei TANG Haoru | 2018 | Horticultural Plant Journal2018，4，3： | 10 |
| 2 | Comparative transcriptome analysis uncovers the regulatory functions of long noncoding RNAs in fruit development and color changes of Fragaria pentaphylla显示文摘To investigate the molecular mechanism underlying fruit development and color change,comparative transcriptome analysis was employed to generate transcriptome profiles of two typical wild varieties of Fragaria pentaphylla at three fruit developmental stages(green fruit stage,turning stage,and ripe fruit stage).We identified 25,699 long noncoding RNAs(lncRNAs)derived from 25,107 loci in the F.pentaphylla fruit transcriptome,which showed distinct stage-and genotype-specific expression patterns.Time course analysis detected a large number of differentially expressed protein-coding genes and lncRNAs associated with fruit development and ripening in both of the F.pentaphylla varieties.The target genes downregulated in the late stages were enriched in terms of photosynthesis and cell wall organization or biogenesis,suggesting that lncRNAs may act as negative regulators to suppress photosynthesis and cell wall organization or biogenesis during fruit development and ripening of F.pentaphylla.Pairwise comparisons of two varieties at three developmental stages identified 365 differentially expressed lncRNAs in total.Functional annotation of target genes suggested that lncRNAs in F.pentaphylla may play roles in fruit color formation by regulating the expression of structural genes or regulatory factors.Construction of the regulatory network further revealed that the low expression of Fra a and CHS may be the main cause of colorless fruit in F.pentaphylla. | Lijun Bai Qing Chen Leiyu Jiang Yuanxiu Lin Yuntian Ye Peng Liu Xiaorong Wang Haoru Tang | 2019 | Horticulture Research2019，6，1： | 5 |
| 3 | Current status and challenges in the application of microbial PHA particles显示文摘Polyhydroxyalkanoates(PHAs)are diversiform biopolyesters with a similar structure and different side chain groups,synthesized by a variety of microorganism.Due to their excellent biodegradability and biocompatibility,PHAs have been used for many applications,including medical implants,antibacterial agents and bioengineering.Nano-architecture is an emerging area for the use of PHAs.This review summarizes the current status and challenges of PHAs-based particles on the micro-and nano-scale,including their production,degradation,biological safety,and surface functionalization.We also focus on the applications of PHA particles in drug delivery systems,environment protection,tissue engi-neering,vaccine engineering,food science,biotechnology and cosmetics.Finally,the future development trends of PHAs-based particles are prospected. | Jiaxuan Liu Ziqi Zhou Haoru Li Xinyu Yang Zeyu Wang jian Xiao Dai-Xu Wei | 2024 | Particuology2024，87，4： | 0 |
| 4 | Comparative metabolomics provides novel insights into the basis of petiole color differences in celery(Apium graveolens L.)显示文摘Plant metabolites are important for plant development and human health.Plants of celery(Apium graveolens L.)with different-colored petioles have been formed in the course of long-term evolution.However,the composition,content distribution,and mechanisms of accumulation of metabolites in different-colored petioles remain elusive.Using ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS),1159 metabolites,including 100 lipids,72 organic acids and derivatives,83 phenylpropanoids and polyketides,and several alkaloids and terpenoids,were quantified in four celery cultivars,each with a different petiole color.There were significant differences in the types and contents of metabolites in celery with different-colored petioles,with the most striking difference between green celery and purple celery,followed by white celery and green celery.Annotated analysis of metabolic pathways showed that the metabolites of the different-colored petioles were significantly enriched in biosynthetic pathways such as anthocyanin,flavonoid,and chlorophyll pathways,suggesting that these metabolic pathways may play a key role in determining petiole color in celery.The content of chlorophyll in green celery was significantly higher than that in other celery cultivars,yellow celery was rich in carotenoids,and the content of anthocyanin in purple celery was significantly higher than that in the other celery cultivars.The color of the celery petioles was significantly correlated with the content of related metabolites.Among the four celery cultivars,the metabolites of the anthocyanin biosynthesis pathway were enriched in purple celery.The results of quantitative real-time polymerase chain reaction(q RT-PCR)suggested that the differential expression of the chalcone synthase(CHS)gene in the anthocyanin biosynthesis pathway might affect the biosynthesis of anthocyanin in celery.In addition,HPLC analysis revealed that cyanidin is the main pigment in purple celery.This study explored the differences in the types and contents of metabolites in celery cultivars with different-colored petioles and identified key substances for color formation.The results provide a theoretical basis and technical support for genetic improvement of celery petiole color. | Mengyao LI Jie LI Haohan TAN Ya LUO Yong ZHANG Qing CHEN Yan WANG Yuanxiu LIN Yunting ZHANG Xiaorong WANG Haoru TANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，4： | 0 |
| 5 | A mechanic insight into low-temperature catalytic combustion toward ethylene oxide over Pt-Ru/CuCeO_(x) bimetallic catalyst显示文摘The catalytic oxidation performance toward ethylene oxide(EO)and the consequent mechanism were investigated on the Pt-Ru/CuCeO_(x)bimetallic catalyst,which was prepared by a distinct method combining stepwise adsorption and subsequent impregnation.The catalytic tests show that the introduction of Ru into the Pt catalyst,so as to form Pt-Ru bimetallic active sites,can greatly increase the oxidation activity of the catalyst,as evidenced by the extremely lower full oxidation temperature(120℃)when compared with that of the Pt/CeO_(2) catalyst(160℃).The XPS spectra show that the Ru species(mainly RuO_(x))have strong interaction with the CuCeO_(x) support,which can therefore affect the electron transfer between the Pt species and the support.As a result,the oxygen activation on Pt species is obviously facilitated and catalytic activity is enhanced.Finally,in situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTs)was used to track the reaction mechanism.It is found that the catalytic oxidation process follows the MvK catalytic mechanism at low temperature and the L-H catalytic mechanism when the temperature moves to higher range. | Wenxi Zhou Kai Chen Quanli Ke Haoru Wang Xiao Chen Yufeng Liu Guokai Cui Xiaole Weng Ying Zhou Hanfeng Lu | 2023 | Journal of Rare Earths2023，41，6： | 0 |
| 6 | Divergent Synthesis of Core m1,Core m2 and Core m3 O-Mannosyi Glycopeptides via a Chemoenzymatic Approach显示文摘O-Mannosylation plays a vital role in the regulation of a variety range of biological processes,for instance,brain and muscle development.However,the precise function remains largely unknown due to its innate heterogeneity.In this regard,it is still welcome to develop efficient methods to access diverse structurally-defined glycopeptides.In this study,a diversity-oriented assembly of O-mannosylα-dystroglycan(α-DG)glycopeptides has been achieved via a chemoenzymatic strategy.This strategy features(i)gram scale divergent synthesis of core m1,core m2 and core m3 mannosylated amino acids from judiciously designed protecting group strategies and chemical glycosidation;(i)efficient glycopeptide assembly via the optimized microwave-assisted solid phase peptide synthesis(SPpS);and(ii)enzymatic elaboration of the core glycan structures to install galactosyl and sialyl-galactosyl moieties.The efficiency and flexibility of this chemoenzymatic approach was demonstrated with the construction of 12 glycopeptides with different core m1,core m2 and core m3 mannosyl glycans,including a core m2 glycopeptide bearing a heptasaccharide for the first time. | Tianlu Li Youqin Zhang Tong Li Haoru Zhuang Fengshan Wang Ning Wang Richard R.Schmidt Peng Peng | 2022 | Chinese Journal of Chemistry2022，40，13： | 0 |
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