|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Function of lncRNAs and approaches to lncRNA-protein interactions显示文摘Long non-coding RNAs(lncRNAs),which represent a new frontier in molecular biology,play important roles in regulating gene expression at epigenetic,transcriptional and post-transcriptional levels.More and more lncRNAs have been found to play important roles in normal cell physiological activities,and participate in the development of varieties of tumors and other diseases.Previously,we have only been able to determine the function of lncRNAs through multiple mechanisms,including genetic imprinting,chromatin remodeling,splicing regulation,mRNA decay,and translational regulation.Application of technological advances to research into the function of lncRNAs is extremely important.The major tools for exploring lncRNAs include microarrays,RNA sequencing(RNA-seq),Northern blotting,real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR),fluorescence in situ hybridization(FISH),RNA interference(RNAi),RNA-binding protein immunoprecipitation(RIP),chromatin isolation by RNA purification(ChIRP),crosslinking-immunopurification(CLIP),and bioinformatic prediction.In this review,we highlight the functions of lncRNAs,and advanced methods to research lncRNA-protein interactions. | ZHU JuanJuan FU HanJiang WU YongGe ZHENG XiaoFei | 2013 | Science China(Life Sciences)2013，56，10： | 54 |
| 2 | Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis显示文摘 | Xu Xiaojie Fan Zhongyi Kang Lei Han Juqiang Jiang Chengying Zheng Xiaofei Zhu Ziman Jiao Huabo Lin Jing Jiang Kai Ding Lihua Zhang Hao Cheng Long Fu Hanjiang Song Yi Jiang Ying Liu Jiahong Wang Rongfu Du Nan Ye Qinong | 2013 | Journal of Clinical Investigation2013，，2： | 7 |
| 3 | Clinical and pathological features of miR-10b and RHOC gene expression in hepatocellular carcinoma显示文摘Aberrant expression of microRNAs(miRNAs)was reported frequently in different human cancers.The major role of miRNA is targeting 30-UTR of coding gene and causing translational repression or mRNA degradation.miR-10b overexpression was reported to promote breast cancer metastasis by up-regulating RHOC expression.But its expression in hepatocellular carcinoma(HCC)remains unclear.Our study indicated that the expression of miR-10b was different in HCC and adjacent tissue samples,and reduced expression of miR-10b in HCC was related tovein invasion.High-level expression of RHOC was also related to vein invasion in HCC.But no correlation was found between miR-10b and RHOC expression.These results suggest that miR-10b and RHOC are independent predictors of HCC invasion and metastasis. | Jingli Du Yulan Wang Hanjiang Fu Xiaofei Zheng Minggui Lin Mingzhou Guo Lixin Wei | 2014 | Chinese Science Bulletin2014，59，19： | 4 |
| 4 | MicroRNA-193b regulates proliferation, migration and invasion in human hepatocellular carcinoma cells显示文摘 | Chengwang Xu Shanshan Liu Hanjiang Fu Shuai Li Yi Tie Jie Zhu Ruiyun Xing Yinghua Jin Zhixian Sun Xiaofei Zheng | 2010 | European Journal of Cancer2010，，15： | 1 |
| 5 | Identification of human fetal liver miRNAs by a novel method显示文摘 | Hanjiang Fu Yi Tie Chengwang Xu Zhuoyuan Zhang Jie Zhu Yingxu Shi Hong Jiang Zhixian Sun Xiaofei Zheng | 2005 | FEBS Letters2005，，17： | 1 |
| 6 | A novel method for ionizing radiation-induced RNA damage detection by poly(A)-tailing RT-PCR显示文摘Ionizing radiation (IR) causes severe cellular damage both directly and indirectly and disrupts RNA integrity. RNA strand breaks are the most frequent type of damage caused by IR. RNA damage is involved in the development of degenerative diseases, including Alzheimer’s disease and Parkinson’s disease. However, the mechanism of mRNA damage and any resulting pathophysiological outcomes are poorly understood. This is partly because there is a lack of sensitive tools to monitor damage randomly occurring in RNA, especially RNA strand break damage in a given RNA. In this work, a method using the reverse transcription polymerase chain reaction (RT-PCR) after poly(A) addition to 3′-end of RNA to determine RNA strand break damage in a specific RNA by poly(A) polymerase has been developed. The levels of damage in specific mRNAs, including ABL1, TP53, GADD45A and ATR from IR-treated HeLa cells were examined. Strand breaks were detected in all mRNAs examined. The study provides a novel and sensitive method based on 3 -end poly(A)-tailing RT-PCR to monitor RNA strand break damage. | TIE Yi HU Zheng LU GuiXiang FU HanJiang XING RuiYun ZHU Jie SUN ZhiXian HENG XiaoFei | 2011 | Chinese Science Bulletin2011，56，30： | 1 |
| 7 | Correlation of microRNAs responding to high dose γ-irradiation with predicted target mRNAs in HeLa cells using microarray analyses显示文摘An increasing data indicates that altered microRNAs(miRNAs)participate in the radiation-induced DNA damage response.However,a correlation of mRNA and miRNA profiles across the entire genome and in response to irradiation has not been thoroughly assessed.We analyzed miRNA microarray data collected from HeLa cells after ionizing radiation(IR),quantified the expression profiles of mRNAs and performed comparative analysis of the data sets using target prediction algorithms,Gene Ontology(GO)analysis,pathway analysis,and gene network construction.The results showed that the altered miRNAs were involved in regulation of various cellular functions.miRNA-gene network analyses revealed that miR-186,miR-106b,miR-15a/b,CCND1and CDK6 played vital role in the cellular radiation response.Using qRT-PCR,we confirmed that twenty-two miRNAs showed differential expression in HeLa cells treated with IR and some of these miRNAs affected cell cycle progression.This study demonstrated that miRNAs influence gene expression in the entire genome during the cellular radiation response and suggested vital pathways for further research. | HU Zheng TIE Yi Lü GuiXiang FU HanJiang XING RuiYun ZHU Jie SUN ZhiXian ZHENG XiaoFei | 2013 | Chinese Science Bulletin2013，58，36： | 1 |
| 8 | MiR-34a inhibits migration and invasion by down-regulation of c-Met expression in human hepatocellular carcinoma cells显示文摘 | Na Li Hanjiang Fu Yi Tie | 2009 | Cancer Letters2009，275，1： | 1 |
| 9 | Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis显示文摘 | Xu Xiaojie Fan Zhongyi Kang Lei Han Juqiang Jiang Chengying Zheng Xiaofei Zhu Ziman Jiao Huabo Lin Jing Jiang Kai Ding Lihua Zhang Hao Cheng Long Fu Hanjiang Song Yi Jiang Ying Liu Jiahong Wang Rongfu Du Nan Ye Qinong | 2013 | Journal of Clinical Investigation2013，，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员