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| 1 | Inhibition of KIT RNAi mediated with adenovirus in gastrointestinal stromal tumor xenograft显示文摘AIM: To investigate a therapeutic method for gastrointestinal stromal tumor (GIST) based on KIT RNA interference (RNAi) with AdMax adenovirus. METHODS: KIT short hairpin RNA (shRNA), whose lateral sides were decorated with restriction endonuclease sequences, was designed. T 4 DNA ligase catalyzed the joint of the KIT shRNA and the green fluorescent protein-containing PDC316-EGFP-U6 to form PDC316EGFP-U6-KIT. Homologous recombination of AdEGFPU6-KIT was performed with the AdMax system. Heterotopically transplanted GISTs were established in nude mice. AdEGFP-U6-KIT was intratumorally injected. The volume, inhibition ratio of tumor and CD117 expression of GIST graft tumor in nude mice were compared between test and control groups. RESULTS: The length of KIT shRNA was determined to be about 50bp by agarose electrophoresis. Gene se-quencing detected the designed KIT RNAi sequence in PDC316-EGFP-U6-KIT. After transfection with AdEGFPU6-KIT, 293 cells displayed green fluorescence. The physical and infective titers of AdEGFP-U6-KIT were 5 × 10 11 viral particles/mL and 5.67 × 10 7 plaque forming units/mL, respectively. The mean volume of the grafted tumor was significantly smaller in test mice than in control mice (75.3 ± 22.9 mm 3 vs 988.6 ± 30.5 mm 3 , t = -18.132, P < 0.05). The inhibition ratio of the tumors was 59.6% in the test group. CD117 positive expression was evident in two cases (20%) in the test group and 10 cases (100%) in the control group (χ 2 = 10.2083, P < 0.005). CONCLUSION: AdEGFP-U6-KIT is successfully constructed, and KIT RNAi mediated with Admax vector system can effectively inhibit the expression of the KIT gene and the growth of GIST in nude mice. | Tian-Bao Wang Wen-Sheng Huang Wei-Hao Lin HanPing Shi Wen-Guang Dong | 2010 | World Journal of Gastroenterology2010，16，40： | 6 |
| 2 | Assessing anxiety,depression,and stress among inpatients with cancer显示文摘Objective This study aimed to assess anxiety,depression,and stress among inpatients with cancer.Methods Two hundred thirty-five hospitalized patients with cancer were surveyed with the Depression Anxiety Stress Scales(simplified Chinese Version).The software program SPSS 25.0 was used for statistical analysis of the survey data.Results The average scores of depression,anxiety,and stress of inpatients with cancer were 12.17,11.84,and 13.98 respectively,which were higher than the normal range.The scores of anxiety and stress of inpatients with different caregivers were statistically different(P=0.024/0.036).The anxiety and stress scores of inpatients with spouses as caregivers were higher than those of inpatients with children as caregivers.There was a statistically significant difference in the incidence of stress between inpatients with cancer with religious beliefs and inpatients with cancer without religious beliefs(P=0.026),and those with religious beliefs had greater incidence of stress.The score of anxiety was significantly higher for inpatients with children than for inpatients without children(P=0.040).Conclusion The anxiety,depression,and stress levels of inpatients with cancer are relatively high.It is necessary to pay special attention to the psychological status of these patients during clinical diagnosis and treatment to improve their quality of life. | Honghong Xu Ruotong Xue Yi Cheng Yujie Zhang Jie Rao Mei Liu Shiying Yu Lingxiang Liu Yiqian Liu Yongqian Shu Liuqing Yang Hanping Shi | 2020 | Oncology and Translational Medicine2020，6，3： | 2 |
| 3 | Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis显示文摘 | ZHANG YUNJIAN SHI HANPING WANG WENJIAN | 2011 | Journal of Vascular Surgery2011，53，3： | 1 |
| 4 | M-CASEngine:acollaborative environment for computer-assisted surgery显示文摘 | Hanping Lufei Weisong Shi Vipin Chaudhary | 2006 | Interna-tional Journal of Computer Assisted Radiology and Surgery2006，1，1： | 1 |
| 5 | Mental health status of cancer caregivers,assessment tools,and psychological interventions显示文摘Many studies pointed out that psychological pain is not limited to the cancer patients themselves,but their caregivers also experience different levels of psychological problems such as depression,anxiety,and stress.This article attempts to review the mental health status,assessment tools,and psychological interventions of the caregivers of cancer patients,and calls on social and medical workers to pay attention to the mental and physical health status of the caregivers of cancer patients. | Honghong Xu Ruotong Xue Yi Cheng Yujie Zhang Jie Rao Mei Liu Shiying Yu Lingxiang Liu Yiqian Liu Yongqian Shu Liuqing Yang Hanping Shi | 2020 | Oncology and Translational Medicine2020，6，4： | 1 |
| 6 | Nutrition status of patients with common cancer in China: gap,mission and challenge显示文摘Malnutrition reduces tolerance and effectiveness of anti-tumor treatment(ANT),increases the risk of complications,medical costs,and death(Arends et al.,2017).About 40%of patients with cancer die because of malnutrition rather than the disease itself(Barton,2017;Roeland et al.,2020).In order to set up a panorama for association among nutrition status,nutrition support treatment(NST),ANT and outcomes in patients with neoplasms,the Chinese Society of Nutritional Oncology(CSNO)conducted a multicenter prospective cohort program called Investigation on Nutrition Status and its Clinical Outcome of Common Cancers(INSCOC)from 2013. | Chunlei Hu Qi Zhang Hanping Shi | 2021 | Science China(Life Sciences)2021，64，11： | 1 |
| 7 | Antithrombotic effect of grape seed proanthocyanidins extract in a rat model of deep vein thrombosis显示文摘 | Yunjian Zhang Hanping Shi Wenjian Wang | 2011 | Journal of Vascular Surgery2011，53，3： | 1 |
| 8 | Mental distress evaluation and intervention for cancer patients显示文摘Mental distress is prevalent among cancer patients.Many measurements have been developed to screen and evaluate such distress.About one-third of the persons with cancer will experience significant levels of distress,requiring targeted psychosocial intervention.Mental distress has been endorsed as the sixth vital sign by the International Psycho-Oncology Society(IPOS) in 2009.The need for effective screening and psychological interventions is well recognized as a necessary,integral part of oncology care.This systematic review examines the psychometric properties of the existing tools used to screen patients for emotional distress and the applicable intervention methods. | Honghong Xu Ruotong Xue Yi Cheng Yujie Zhang Jie Rao Mei Liu Shiying Yu Lingxiang Liu Yiqian Liu Yongqian Shu Liuqing Yang Hanping Shi | 2019 | Oncology and Translational Medicine2019，5，6： | 0 |
| 9 | Secure Application-Aware Service Differentiation in Public Area Wireless Networks显示文摘We are witnessing the increasing demand for pervasive Internet access from public area wireless networks (PAWNs). As their popularity grows, the inherent untrusted nature of public places and the diverse service requirements of end users are two key issues that need to be addressed. We have proposed two approaches to address these issues. First,the Home-based Authentication Protocol (HAP) that provides a framework by which to establish trust between a nomadic client and a service provider using a trusted third party (home). Second, we argue that the best-effort-based service model provided by many access points is not enough to satisfy the end user fairness and to maximize the wireless link utilization for a diverse user population. We have proposed an application-aware service differentiation (AASD) mechanism that takes both application semantics and user requirements into consideration. Our analysis of this framework shows several fruitful results. The total authentication latency increases with the number of clients but at a rate that is much less than linear increasing latency. Also, in comparison with two other bandwidth allocation approaches, the best effort and static access control, our proposed application-aware service differentiation method, outperforms them in terms of the client fairness and wireless bandwidth utilization. | Weisong Shi Sharun Santhosh Hanping Lufei | 2005 | Journal of Computer Science & Technology2005，20，5： | 0 |
| 10 | Nutrition in Alzheimer's disease:a review of an underappreciated pathophysiological mechanism显示文摘Alzheimer's disease(AD)is the leading cause of dementia in older individuals and is an escalating challenge to global public health.Pharmacy therapy of AD is one of the well-funded areas;however,little progress has been made due to the complex pathogenesis.Recent evidence has demonstrated that modifying risk factors and lifestyle may prevent or delay the incidence of AD by 40%,which suggests that the management should pivot from single pharmacotherapy toward a multipronged approach because AD is a complex and multifaceted disease.Recently,the gut-microbiota-brain axis has gained tremendous traction in the pathogenesis of AD through bidirectional communication with multiple neural,immune,and metabolic pathways,providing new insights into novel therapeutic strategies.Dietary nutrition is an important and profound environmental factor that influences the composition and function of the microbiota.The Nutrition for Dementia Prevention Working Group recently found that dietary nutrition can affect cognition in AD-related dementia directly or indirectly through complex interactions of behavioral,genetic,systemic,and brain factors.Thus,considering the multiple etiologies of AD,nutrition represents a multidimensional factor that has a profound effect on AD onset and development.However,mechanistically,the effect of nutrition on AD is uncertain;therefore,optimal strategies or the timing of nutritional intervention to prevent or treat AD has not been established.Thus,this review summarizes the current state of knowledge concerning nutritional disorders,AD patient and caregiver burden,and the roles of nutrition in the pathophysiology of AD.We aim to emphasize knowledge gaps to provide direction for future research and to establish optimal nutrition-based intervention strategies for AD. | Jiwei Jiang Hanping Shi Shirui Jiang Anxin Wang Xinying Zou Yanli Wang Wenyi Li Yuan Zhang Mengfan Sun Qiwei Ren Jun Xu | 2023 | Science China(Life Sciences)2023，66，10： | 0 |
| 11 | AIM-CICs: an automatic identification method for cell-in-cell structures based on convolutional neural network显示文摘Whereas biochemical markers are available for most types of cell death, current studies on non-autonomous cell death by entosis rely strictly on the identification of cell-in-cell structures (CICs), a unique morphological readout that can only be quantified manually at present. Moreover, the manual CIC quantification is generally over-simplified as CIC counts, which represents a major hurdle against profound mechanistic investigations. In this study, we take advantage of artificial intelligence technology to develop an automatic identification method for CICs (AIM-CICs), which performs comprehensive CIC analysis in an automated and efficient way. The AIM-CICs, developed on the algorithm of convolutional neural network, can not only differentiate between CICs and non-CICs (the area under the receiver operating characteristic curve (AUC) > 0.99), but also accurately categorize CICs into five subclasses based on CIC stages and cell number involved (AUC > 0.97 for all subclasses). The application of AIM-CICs would systemically fuel research on CIC-mediated cell death, such as high-throughput screening. | Meng Tang Yan Su Wei Zhao Zubiao Niu Banzhan Ruan Qinqin Li You Zheng Chenxi Wang Bo Zhang Fuxiang Zhou Xiaoning Wang Hongyan Huang Hanping Shi Qiang Sun | 2022 | Journal of Molecular Cell Biology2022，14，6： | 0 |
| 12 | Ensemble learning system to identify nutritional risk and malnutrition in cancer patients without weight loss information显示文摘Dear Editor,Malnutrition is a prevalent disease in oncology practice(Arends et al.,2017).With its cancer-specific prevalence ranging from 21%–72%,malnutrition is responsible for 10%–20% cancer deaths(Yin et al.,2021b).However,malnutrition is often underestimated,misclassified,or left untreated in cancer care(Hébuterne et al.,2014). | Liangyu Yin Jie Liu Ming Liu Jin Zheng Aiguo Xu Quanjun Lyu Yongdong Feng Zengqing Guo Hu Ma Jipeng Li Zhikang Chen Hui Wang Zengning Li Chunling Zhou Xi Gao Min Weng Qinghua Yao Wei Li Tao Li Hanping Shi Hongxia Xu | 2023 | Science China(Life Sciences)2023，66，5： | 0 |
| 13 | Diagnosis of malnutrition in cancer patients显示文摘Malnutrition is a common complication in patients with malignant tumors and adversely affects treatment and prognosis1.It has been reported that the global prevalence of malnutrition in hospitalized patients with malignant tumors is approximately 70%2.Moreover,approximately 20%of cancer patient deaths are directly attributable to malnutrition3.According to the Investigation on Nutrition Status and its Clinical Outcome of Common Cancers(INSCOC)project4,the overall prevalence of malnutrition in Chinese inpatients with common malignant tumors is 80.4%.Of the 58. | Chunhua Song Hanping Shi | 2023 | Cancer Biology & Medicine2023，20，12： | 0 |
| 14 | Highly sensitive detection of daminozide using terahertz metamaterial sensors显示文摘In order to solve the problems of low sensitivity and complex sample pretreatment in traditional detection,a method was proposed in this study to detect daminozide using terahertz combined with a metamaterial sensor,which enables real-time and label-free molecular detection with high sensitivity.The correlation between the transmission frequency shift and absorbance of daminozide solution at different concentrations was analyzed.The simulation and experimental results showed that this metamaterial sensor could achieve highly sensitive sensing of daminozide solutions at 0.05 mg/L.The maximum quality factor(Q_(max))of the two peaks could reach 5.78 and 13.05,and the maximum figure of merit(FOM)of the two peaks can reach 0.82 and 1.72.The maximum sensitivity of two resonance peaks reached 38.148 GHz/(mg·L)and 133.516 GHz/(mg·L)when the concentration of daminozide in the solution was 2000 mg/L.There was an obvious positive correlation between the transmission and transmission,frequency shift and frequency shift,and absorbance and absorbance of the resonance peak of daminozide solutions.Therefore,this platform not only opens up new possibilities for the microanalysis of the chemical composition of substances in solutions but also provides a valuable reference for the design of other metamaterial-based sensors in the field of food safety. | Hanping Mao Xiaoxue Du Yuting Yan Xiaodong Zhang Guoxin Ma Yafei Wang Yong Liu Bin Wang Xiaoyue Yang Qiang Shi | 2022 | International Journal of Agricultural and Biological Engineering2022，15，6： | 0 |
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