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| 1 | Integrin betal mediates hepatocellular carcinoma cells chemotaxis to laminin显示文摘BACKGROUND: Chemotaxis is an important step durin the invasion of carcinoma cells. And integrins are most im portant receptors mediating interaction between cells an extracellular matrix ( ECM). This study was designed t study integrin betal mediating chemotaxis of hepatocellula carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to in vestigate the effect of blockade of integrin betal on pseudo pod protrusion of HCC cells in response to LN stimulation Chemotactic pseudopod protrusion of a HCC cell was eva luated using a dual-pipette set-up, in which two pipette filled with LN solution were positioned in close contact wit the same cell, and pseudopod protrusion into each pipett was viewed dynamically and recorded with a tape recorder The lengths of pseudopods were measured and plotte against time to obtain a pseudopod growth curve. The in tegrin betal subunit on the surfaces of HCC cells were ana lyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, whe the two pipettes were filled with LN(50 μg/ml, 200 μg/ml) pseudopods extended from the HCC cell into each of th pipettes nearly symmetrically, ie, with nearly identica maximum pseudopod length and similar pseudopod growth curves. Upon addition of anti-CD29 (20 μg/ml) to one o the pipettes, pseudopod protrusion was blocked nearly completely while protrusion into the opposite pipette be came more evidently, with a larger maximum length. Ex pression of integrin betal was up to 95.78% to cells chosen in the experiment. CONCLUSION: Integrin betal subunit was an importan constituent receptor subunit for mediating chemotactic pseudopod protrusion of HCC cell to LN. | Bian-Hong Fu, Ze-Zhi Wu and Cheng Dong Chongqing, China College of Bioengineering, Key Lab for Biomechanics and Tissue Engineering under the State Ministry of Education and College of Resources & Environmental Science , Chongqing University, Chongqing 400044, China Bioengineering Pro- gram, Pennsylvania State University, 205 Hallowell Building, University Park, Pennsylvania 16802-6804, USA | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 4 |
| 2 | Intemal Service Quality, Customer and Job Satisfaction: Linkages and Implications for Management显示文摘 | Hallowell R Schlesinger L A Zornitsdy J | 1996 | Human Resource Planning1996，19，4： | 1 |
| 3 | A comparison of twodoses of omeprazole in the treatment of equine gastric ulcersyndrome: A blinded, randomised, clinical trial显示文摘 | Sykes B W Sykes KM Hallowell G D | 2014 | Equine VeterinaryJournal2014，46，4： | 1 |
| 4 | The use of technologies to support human milk &breastfeeding 显示文摘 | Froh EB Hallowell S Spatz DL | 2015 | J Pediatr Nurs2015，30，3： | 1 |
| 5 | Revisiting Thalidomide:fighting with caution against idiopathic pulmonary fibrosi显示文摘 | Druhorton M R Hallowell | 2012 | Drugs Today (Barc)2012，48，10： | 1 |
| 6 | The relationship of customer satisfaction customer loyalty,profitability:an empirical study显示文摘 | Hallowell R | 1996 | International Journal of Service Industry Management1996，7，4： | 1 |
| 7 | The relationships of customer satisfaction, customer loyalty, and profitability: An empirical study显示文摘 | Hallowell R | 1996 | International Journal of Service Industry Management1996，7，4： | 1 |
| 8 | Internal service quality, customer and job satisfaction : Linkages and implications for management显示文摘 | Hallowell R Schlesinger L A Zornitsky J | 1996 | Human Resource Planning1996，19，2： | 1 |
| 9 | Health promotion by flavonoids,tocopherols,tocotrienols and other phenols:direct or indirect effects? Antioxidants or not?显示文摘 | HALLOWELL B RAFTER J JENNER A | 2005 | Am J Clin Nutr2005，81，1： | 1 |
| 10 | Fate of Infants With Hypoplastic Left Heart Syndrome Listed for Cardiac Transplantation: A Multicenter Study显示文摘 | M.R.K. Chrisant D.C. Naftel J. Drummond-Webb R. Chinnock C.E. Canter M.M. Boucek R.J. Boucek S.C. Hallowell J.K. Kirklin W.R. Morrow | 2005 | Journal of Heart and Lung Transplantation2005，，5： | 1 |
| 11 | Internal Service Quality, Customer and Job Satisfaction:Linkage and Implication for Management 显示文摘 | Hallowell | 1996 | Human Resource Planning1996，，19： | 1 |
| 12 | Idiopathic colitis following cardiac transplantation: Three pediatric cases显示文摘 | Wahbeh G Hupertz V Hallowell S | 2003 | Pediatr Transplant2003，7，6： | 1 |
| 13 | Humberto Matumna and Francisco Varela's Contribution to Media Ecology:Autopoiesis,the Santiago School of Cognition,and Enactive Cognitive Science显示文摘 | Hallowell R | | 0，，： | 1 |
| 14 | The Relationship of Customer Satisfaction, Customer Loyalty, and Profitability : An Empirical Study显示文摘 | Hallowell R | 1996 | Interna- tional Journal of service Industry Management1996，7，4： | 1 |
| 15 | Construction-safety best practices and relationships to safety performance显示文摘 | Hinze J Hallowell M Baud K | 2013 | Journal of Construction Engineering & Management2013，139，10： | 1 |
| 16 | Risk-based framework 'for safety investment in construction organizations显示文摘 | Hallowell M | 2011 | Journal of Construction Engi- neering & Management2011，137，8： | 1 |
| 17 | Enabling and measur- ing innovation in the construction industry显示文摘 | GAMBATESE J HALLOWELL M | 2011 | Construction Management and Economics2011，29，6： | 1 |
| 18 | Diffusion of safety inno- vations in the construction industry显示文摘 | ESMAEILI B HALLOWELL M R | 2012 | Journal of Construction Engineering and Management2012，138，8： | 1 |
| 19 | Safety-Knowledge Management in A- merican Construction Organizations 显示文摘 | Hallowell M R | 2011 | Journal of Management in Engineering2011，28，2： | 1 |
| 20 | 2004 where do we go from here? -Summary of working group discussions on thyroid function and gestational outcomes 显示文摘 | Hallowell JG LaFranehi S Smallridge RC | 2005 | Thyroid2005，15，1： | 1 |
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