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| 1 | Intracellular distribution of amyloid beta peptide and its relationship to the lysosomal system显示文摘Background:Amyloid beta peptide(Aβ)is the main component of extraneuronal senile plaques typical of Alzheimer’s disease(AD)brains.Although Aβis produced by normal neurons,it is shown to accumulate in large amounts within neuronal lysosomes in AD.We have recently shown that under normal conditions the majority of Aβis localized extralysosomally,while oxidative stress significantly increases intralysosomal Aβcontent through activation of macroautophagy.It is also suggested that impaired Aβsecretion and resulting intraneuronal increase of Aβcan contribute to AD pathology.However,it is not clear how Aβis distributed inside normal neurons,and how this distribution is effected when Aβsecretion is inhibited.Methods:Using retinoic acid differentiated neuroblastoma cells and neonatal rat cortical neurons,we studied intracellular distribution of Aβby double immunofluorescence microscopy for Aβ40 or Aβ42 and different organelle markers.In addition,we analysed the effect of tetanus toxin-induced exocytosis inhibition on the intracellular distribution of Aβ.Results:Under normal conditions,Aβwas found in the small cytoplasmic granules in both neurites and perikarya.Only minor portion of Aβwas colocalized with trans-Golgi network,Golgi-derived vesicles,early and late endosomes,lysosomes,and synaptic vesicles,while the majority of Aβgranules were not colocalized with any of these structures.Furthermore,treatment of cells with tetanus toxin significantly increased the amount of intracellular Aβin both perikarya and neurites.Finally,we found that tetanus toxin increased the levels of intralysosomal Aβalthough the majority of Aβstill remained extralysosomally.Conclusion:Our results indicate that most Aβis not localized to Golgi-related structures,endosomes,lysosomes secretory vesicles or other organelles,while the suppression of Aβsecretion increases intracellular intra-and extralysosomal Aβ. | Lin Zheng Angel Cedazo-Minguez Martin Hallbeck Fredrik Jerhammar Jan Marcusson Alexei Terman | 2012 | Translational Neurodegeneration2012，1，1： | 10 |
| 2 | Preproenkephalin mRNA expression neurons in the rat thalamus 显示文摘 | HERMANSON O HALLBECK M BLOMQVIST A | 1995 | Neuroreport1995，6，6： | 1 |
| 3 | Quantification of the effects of instruc- tion type, verbal encouragement, and visual feedback on static and peak handgrip strength 显示文摘 | Jung MC Hallbeck MS | 2004 | Int J Industrial Ergonomics2004，34，： | 1 |
| 4 | Benefits associated with the stalk of GallioneUa ferruginea, evaluated by comparison of a stalkforming and non-stalk-forming strain and biofilm studies in situ 显示文摘 | Hallbeck L Pedersen K | 1995 | Microbial Ecology1995，30，： | 1 |
| 5 | Interleukin-6 Enhances Transforming Growth Factor-alpha mRNA Expression in Macrophage-Like Human Monocytoid (U-937-1) Cells显示文摘 | A. L. Hallbeck T. M. Walz A. Wasteson | 2001 | Bioscience Reports2001，，3： | 1 |
| 6 | Distribution of preprovasopressin mRNA in the rat central nervous system显示文摘 | Hermanson O Blomquist A | 1999 | J Com Neurol1999，411，2： | 1 |
| 7 | Dynorphin mRNA-expressing neurons in the rat paraventricular hypothalamic nucleus project to the spinal cord 显示文摘 | Hallbeck M | 2000 | Neurosci Lett2000，285，3： | 1 |
| 8 | Long-term treatment with antidepressants,but not environmental stimulation,induces expression of np2 mrna in hippocampus and medial habenula显示文摘 | Bjartmar L Alkhori L Ruud J Mohammed A.H Marcusson J & Hallbeck M | | 0，，： | 1 |
| 9 | Preproenkephalin mRNA expression neurons in the rat thalamus显示文摘 | Hermanson O Hallbeck M blomqvist A | 1995 | Neuroreport1995，6，： | 1 |
| 10 | Preprocnkephalin mRNA expression neurons in the rat thalamus 显示文摘 | Hallbeck M Blomqvist A | 1995 | Neuroreport1995，6，： | 1 |
| 11 | Fe(III)distribution varies substantially within and betweenatherosclerotic plaques 显示文摘 | GUSTAFSSON H HALLBECK M NORELL M | 2014 | Magn Reson Med2014，71，2： | 1 |
| 12 | Quantification of the effects of instruction type,verbal encouragement,and visual feedback on static and peak handgrip strength显示文摘 | Myung-Chul Jung M Susan Hallbeck | | 0，，： | 1 |
| 13 | Interleukin-6 enhances transforming growth factor-alpha mRNA expression in macrophage-like human monocytoid (U-937-1) cells显示文摘 | Hallbeck AL Walz TM Wasteson A | 2001 | Biosci Rep2001，21，3： | 1 |
| 14 | Quantification of the effects of instruction type, verbal encouragement, and visual feedback on static and peak handgrip strength 显示文摘 | Jung MC Hallbeck MS | 2004 | Int J Industrial Ergonomics2004，34，5： | 1 |
| 15 | Characterization of microbial processes in deep aquifers of the Fennoscandian Shield显示文摘 | Hallbeck L and Pedersen K | 2008 | Applied Geochemistry2008，23，： | 1 |
| 16 | Characterization of microbial processes in deep aquifers of the Fennoscandian Shield显示文摘 | Hallbeck L Pedersen K | 2008 | Applied Geochemistry2008，23，： | 1 |
| 17 | Evaluation of four cursor control devices during a target acquisition task for lap- aroscopic tool control 显示文摘 | Herring S R Trejo A E Hallbeck M S | 2010 | Applied Ergonomics2010，41，1： | 1 |
| 18 | Distribution of preprovasopressin mRNA in the rat central nervous system显示文摘 | Hallbeck M Hermanson O Blomquist A | 1999 | J Com Neurol1999，411，2： | 1 |
| 19 | Distribution of preprov- asopressin mRNA in the rat central nervous system 显示文摘 | Hallbeck M Hermanson O Blomqvist A | 1999 | J Comp Neurol1999，411，2： | 1 |
| 20 | Laparoendoscopic single-site (LESS) surgery versus conventional laparoscopic surgerycom- parison of surgical port performance in a surgical simulator with novices显示文摘 | Brown-Clerk B de Laveaga A E LaGrange C A Wirth L M Lowndes B R Hallbeck M S | 2011 | Surg Endosc2011，25，： | 1 |
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