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| 1 | Intracellular distribution of amyloid beta peptide and its relationship to the lysosomal system显示文摘Background:Amyloid beta peptide(Aβ)is the main component of extraneuronal senile plaques typical of Alzheimer’s disease(AD)brains.Although Aβis produced by normal neurons,it is shown to accumulate in large amounts within neuronal lysosomes in AD.We have recently shown that under normal conditions the majority of Aβis localized extralysosomally,while oxidative stress significantly increases intralysosomal Aβcontent through activation of macroautophagy.It is also suggested that impaired Aβsecretion and resulting intraneuronal increase of Aβcan contribute to AD pathology.However,it is not clear how Aβis distributed inside normal neurons,and how this distribution is effected when Aβsecretion is inhibited.Methods:Using retinoic acid differentiated neuroblastoma cells and neonatal rat cortical neurons,we studied intracellular distribution of Aβby double immunofluorescence microscopy for Aβ40 or Aβ42 and different organelle markers.In addition,we analysed the effect of tetanus toxin-induced exocytosis inhibition on the intracellular distribution of Aβ.Results:Under normal conditions,Aβwas found in the small cytoplasmic granules in both neurites and perikarya.Only minor portion of Aβwas colocalized with trans-Golgi network,Golgi-derived vesicles,early and late endosomes,lysosomes,and synaptic vesicles,while the majority of Aβgranules were not colocalized with any of these structures.Furthermore,treatment of cells with tetanus toxin significantly increased the amount of intracellular Aβin both perikarya and neurites.Finally,we found that tetanus toxin increased the levels of intralysosomal Aβalthough the majority of Aβstill remained extralysosomally.Conclusion:Our results indicate that most Aβis not localized to Golgi-related structures,endosomes,lysosomes secretory vesicles or other organelles,while the suppression of Aβsecretion increases intracellular intra-and extralysosomal Aβ. | Lin Zheng Angel Cedazo-Minguez Martin Hallbeck Fredrik Jerhammar Jan Marcusson Alexei Terman | 2012 | Translational Neurodegeneration2012，1，1： | 10 |
| 2 | Preclinical and clinical pharmacolgy of antisense oligonucleotides显示文摘 | Marcusson EG yacyshyn BR Shanahan WJ | 1999 | Mol Biotechnol1999，12，1： | 1 |
| 3 | Mutant prevention concentration of ciprofloxacin for urinary tract infection isolates of Escherichia coli 显示文摘 | Marcusson LL Olofsson SK Komp Lindgren P | 2005 | J Antimicrob Chemother2005，55，6： | 1 |
| 4 | Prooncogenlc factors miR-23b and miR-27b are regulated by Her2/Neu, EGF, and TNF-alpha in breast cancer 显示文摘 | JIN L WESSELY O MARCUSSON EG | 2013 | Cancer Res2013，73，9： | 1 |
| 5 | Caudate nucleus dopamine D2 receptors in vascular dementia显示文摘 | Allard P England E Marcusson J | 2002 | Dementia Geriatr Cogn Disord2002，14，1： | 1 |
| 6 | Oxidative stress and Alzheimer disease: the autophagy connection? 显示文摘 | Zheng L Marcusson J Terman A | 2006 | Auto- phagy2006，2，2： | 1 |
| 7 | Selection of ciprofloxacin resistance in Escherichia coli in an in vitro kinetic model: relation between drug exposure and mutant prevention concentration显示文摘 | Olofsson S K Marcusson L L Lindgren P K et aI | 2006 | J Antimic Chemother2006，57，6： | 1 |
| 8 | Prooncogenic factors miR- 23b and miR-27b are regulated by Her2/Neu, EGF, and TNF-alpha in breast cancer 显示文摘 | Jin L Wessely O Marcusson EG | 2013 | Cancer Res2013，73，9： | 1 |
| 9 | Selection of ciprofloxacin resistance in Escherichia coli in an in vitro kinetic model:relation between drug exposure and mutant prevention concentration显示文摘 | Olofsson SK Marcusson LL Komp-Lindgren P | 2006 | J Antimicrob Chemother2006，57，6： | 1 |
| 10 | MicroRNA-143 regulates adipocyte differentiation 显示文摘 | Esau C Kang X Peralta E Hanson E Marcusson EG Ravichandran LV | 2004 | J Biol Chem2004，279，52： | 1 |
| 11 | Prooneogenic factors miR- 23b and miR-27b are regulated by Her2/Neu, EGF, and TNF-alpha in breast cancer 显示文摘 | Jin L Wessely O Marcusson EG | | Cancer Res0，73，9： | 1 |
| 12 | Biological Cost of Single and Multiple Norfloxacin Resistance Mutations in Escherichia coli Implicated in Urinary Tract Infections 显示文摘 | Patricia Komp Lindgren Linda L Marcusson Dorthe Sandvang | 2005 | Antimicrob Agents Chemother2005，49，6： | 1 |
| 13 | Endosome to golgi retrieval of the vacuolar protein sorting receptor, Vps10p, requires the function of the VPS29, VPS30, and VPS35 gene products 显示文摘 | Seaman MN Marcusson EG Cereghino JL | 1997 | J Cell Biol1997，137，1： | 1 |
| 14 | White spotreduction when using glass ionomer cement for bonding in orthodontics:a longitudinal and comparative study显示文摘 | Marcusson A Norevall LI Persson M | | 0，，： | 1 |
| 15 | Prooncogenic factors miR-23b and miR-27b are regulated by Her2/Neu, EGF, and TNF-alpha in breast cancer 显示文摘 | Jin L Wessely O Marcusson EG | 2013 | Cancer Res2013，73，9： | 1 |
| 16 | Dose-relatedselection of fluoroquinolone-resistant Escherichia coli显示文摘 | Olofsson S K Marcusson L L Strmbck A | 2007 | Journalof Antimicrobial Chemotherapy2007，60，： | 1 |
| 17 | Galantamine demonstrates efficacy and safety in elderly patients with Alzheimer disease显示文摘 | Marcusson J Bullock R Gauthier S | 2003 | Alzheimer Dis Assoc Disord2003，17，： | 1 |
| 18 | MicroRNA-143 regulates adipocyte differentiation显示文摘 | Esau C Kang X Peralta E Hanson E Marcusson EG Ravichandran LV | 2004 | J Bio Chem2004，279，52： | 1 |
| 19 | Selection of ciprofloxacin resistance in Escherichia coli in an in vitro k-inetic model:rlation between drug exposure and mutant prevention concentration显示文摘 | Olofsson SK Marcusson LL Komp Lindgren P | 2006 | J Antimicrob Chemother2006，57，6： | 1 |
| 20 | Improved client participation in the rehabilitation process using a client-centred goal formulation structure显示文摘 | Wressle E Eeg-Olofsson AM Marcusson J | 2002 | J Rehabil Med2002，34，1： | 1 |
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