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1316篇 您的检索式：作者名="HOLCOMBE"

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	题名	作者	年代	出处	被引量
1	Oxidative stress-induced mitochondrial dysfunction in a normal colon epithelial cell line显示文摘AIM To determine how a normal human colon cell line reacts to microbial challenge as a way to study oxidative stress-induced responses associated with inflammatory bowel disease.METHODS Normal human colon epithelial cells(ATCC?CRL.1790?)were stimulated with either heat killed E.coli or heat killed murine cecal contents(HKC)and examined for several relevant biomarkers associated with inflammation and oxidative stress including cytokine production,mitochondrial autophagy and oxidant status.TNFα,IL-1βand IL-8 protein concentrations were measured within the supernatants.Fluorescent microscopy was performed to quantify the production of reactive oxygen species(ROS)using an oxidation responsive fluorogenic probe.Mitochondrial morphology and mitochondrial membrane potential was assessed by dual staining using COXIV antibody and a dye concentrating in active mitochondria.Mitochondrial ROS scavenger was used to determine the source of ROS in stimulated cells.Autophagy was detected by staining for the presence of autophagic vesicles.Positive controls for autophagy and ROS/RNS experiments were treated with rapamycin and chloroquine.Mitochondrial morphology,ROS production and autophagy microscopy experiments were analyzed using a custom acquisition and analysis microscopy software(Image J).RESULTS Exposing CRL.1790 cells to microbial challenge stimulated cells to produce several relevant biomarkers associated with inflammation and oxidative stress.Heat killed cecal contents treatment induced a 10-12fold increase in IL-8 production by CRL.1790 cells compared to unstimulated controls at 6 and 12 h(P<0.001).Heat killed E.coli stimulation resulted in a4-5 fold increase in IL-8 compared to the unstimulated control cells at each time point(P<0.001).Both heat killed E.coli and HKC stimulated robust ROS production at 6(P<0.001),and 12 h(P<0.01).Mitochondrial morphologic abnormalities were detected at 6 and12 h based on reduced mitochondrial circularity and decreased mitochondrial membrane potential,P<0.01.Microbial stimulation also induced significant autophagy at 6 and 12 h,P<0.01.Lastly,blocking mitochondrial ROS generation using mitochondrial specific ROS scavenger reversed microbial challenge induced mitochondrial morphologic abnormalities and autophagy.CONCLUSION The findings from this study suggest that CRL.1790cells may be a useful alternative to other colon cancer cell lines in studying the mechanisms of oxidative stress events associated with intestinal inflammatory disorders.	Nandakumar Packiriswamy Kari F Coulson Susan J Holcombe Lorraine M Sordillo	2017	World Journal of Gastroenterology2017，23，19：	3
2	Adhesive small bowel obstruction after appendectomy in children: comparison between the laparoscopic and open approach显示文摘	Kuo Jen Tsao Shawn D. St Peter Patricia A. Valusek Scott J. Keckler Susan Sharp George W. Holcomb Charles L. Snyder Daniel J. Ostlie	2007	Journal of Pediatric Surgery2007，，6：	2
3	Sarcopenia and Mortality after Liver Transplantation显示文摘	Michael J. Englesbe Shaun P. Patel Kevin He Raymond J. Lynch Douglas E. Schaubel Calista Harbaugh Sven A. Holcombe Stewart C. Wang Dorry L. Segev Christopher J. Sonnenday	2010	Journal of the American College of Surgeons2010，，2：	2
4	Damage control resuscitation:directly addressing the early coagulopathy of trauma显示文摘	Holcomb JB Jenkins D Rhee P	2007	Journal of Trauma-Injury Infection and Critical Care2007，，02：	2
5	Resuscitation and transfusion principles for traumatic hemorrhagic shock显示文摘	Philip C. Spinella John B. Holcomb	2009	Blood Reviews2009，，6：	2
6	Moisture sorption and transport in clothing during wear 显示文摘	BARNES J C HOLCOMBE B V	1996	Journal of Textile Research1996，66，12：	1
7	Relaxation and microfracturing in dilatants rock显示文摘	HOLCOMB D J	1981	Journal of Geophysical Research1981，86，7：	1
8	Toward a model of strategic outsourcing显示文摘	Holcomb T R Hitt M A	2007	Journal of Operations Management2007，25，2：	1
9	Why corals care about ocean acidification: Uncovering the mechanism显示文摘	Cohen A L Holcomb M	2009	Oceanography2009，22，4：	1
10	Introducing X-machinemodels to verify PLC ladder diagrams显示文摘	Chambers C Holcombe M Barnard J	2001	Computers in Industry2001，45，3：	1
11	Further investigation of spreader loads required to cause vertical root fracture during lateral condensation显示文摘	Holcomb JQ Pitts DL Nicholls JI	1987	Endod1987，13，6：	1
12	Prostate stem cell antigen as therapy tarot:tissue expression and in vivo efficacy of an immunoconjugate 显示文摘	Ross S Spencer SD Holcomb I	2002	Cancer Res2002，62，9：	1
13	Pediatric laparoscopic splenectomy显示文摘	Tulman S Holcomb GW Karamanoukian HL	1993	J Pediatr Surg1993，28，5：	1
14	Human error and commercial aviation accidents: An analysis using the human factors analysis and classification system显示文摘	SHAPPELL S DETWILER C HOLCOMB K	2007	Human Factors2007，49，2：	1
15	Accelerated Alzheimertype phenotype in transgenic mice carrying both mutant amyloid precursor protein and presenilin-1 transgenes显示文摘	Holcomb L Gordon MN Mc Gowan E	1998	Nat Med1998，4，1：	1
16	The virtual nose: a 3-dim- ensional virtual reality model of human nose显示文摘	Vartanion A J Holcomb J Ai Z	2004	Arch Facial P1- ast Surg2004，6，5：	1
17	Laparoscopic esophago- myotomy for aehalasia in children 显示文摘	Holcomb GW 3rd Richards WO Riede] BD	1996	J Pediatr Surg1996，31，5：	1
18	Damage control resuscitation: directly addressing the early eoagulopathy of trauma 显示文摘	Holcomb JB Jenkins D Rhee P	2007	J Trauma2007，62，2：	1
19	Angiogenesis in early-invasive and low-malignant-poptential epithelial carcinoma显示文摘	Abulafia O Ruiz JE Holcomb K	2000	Obstet Gynecol2000，95，4：	1
20	Using acoustic emissions to determine in-situ stress: problems and promise 显示文摘	Holcomb D J	1983	Geomechanics ASME1983，57，：	1

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