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| 1 | Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming显示文摘Grapevine is a perennial crop often cultivated by grafting a scion cultivar on a suitable rootstock.Rootstocks influence scions,particularly with regard to water uptake and vigor.Therefore,one of the possibilities to adapt viticulture to the extended drought stress periods is to select rootstocks conferring increased tolerance to drought.However,the molecular mechanisms associated with the ability of rootstock/scion combination to influence grape berry metabolism under drought stress are still poorly understood.The transcriptomic changes induced by drought stress in grape berries(cv.Pinot noir)from vines grafted on either 110R(drought-tolerant)or 125AA(drought-sensitive)rootstock were compared.The experiments were conducted in the vineyard for two years and two grape berry developmental stages(50%and 100%veraison).The genome-wide microarray approach showed that water stress strongly impacts gene expression in the berries,through ontology categories that cover cell wall metabolism,primary and secondary metabolism,signaling,stress,and hormones,and that some of these effects strongly depend on the rootstock genotype.Indeed,under drought stress,berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate(JA),phenylpropanoid metabolism,and pathogenesis-related proteins.The data also suggest a link between JA and secondary metabolism in water-stressed berries.Overall,genes related to secondary metabolism and JA are more induced and/or less repressed by drought stress in the berries grafted on the drought-sensitive rootstock 125AA.These rootstock-dependent gene expression changes are relevant for berry composition and sensory properties. | Mariam Berdeja Philippe Nicolas Christian Kappel Zhan Wu Dai Ghislaine Hilbert Anthony Peccoux Magali Lafontaine Nathalie Ollat Eric Gomès Serge Delrot | 2015 | Horticulture Research2015，2，1： | 5 |
| 2 | Treatment of metastatic colorectal carcinomas by systemic inhibition of vascular endothelial growth factor signaling in mice显示文摘AIM: Tumor angiogenesis has been shown to be promoted by vascular endothelial growth factor (VEGF) via stimulating endothelial cell proliferation, migration, and survival. Blockade of VEGF signaling by different means has been demonstrated to result in reduced tumor growth and suppression of tumor angiogenesis in distinct tumor entities. Here, we tested a recombinant adenovirus, AdsFIt1-3, that encodes an antagonistically acting fragment of the VEGF receptor 1 (Flt-1), for systemic antitumor effects in pre-established subcutaneous CRC tumors in mice. METHODS: Murine colorectal carcinoma cells (CT26) were inoculated subcutaneously into Balb/c mice for in vivo studies. Tumor size and survival were determined. 293 cell line was used for propagation of the adenoviral vectors. Human lung cancer line A549 and human umbilical vein endothelial cells were transfected for in vitro experiments. RESULTS: Infection of tumor cells with AdsFlt1-3 resulted in protein secretion into cell supernatant, demonstrating correct vector function. As expected, the secreted sFlt1-3 protein had no direct effect on CT26 tumor cell proliferation in vitro, but endothelial cell function was inhibited by about 46% as compared to the AdLacZ control in a tube formation assay. When AdsFlt1-3 (5×109 PFU/animal) was applied to tumor bearing mice, we found a tumor inhibition by 72% at d 12 after treatment initiation. In spite of these antitumoral effects, the survival time was not improved. According to reduced intratumoral microvessel density in AdsFIt1-3-treated mice, the antitumor mechanism can be attributed to angiostatic vector effects. We did not detect increased systemic VEGF levels after AdsFlt1-3 treatment and liver toxicity was low as judged by serum alanine aminotransferase determination. CONCLUSION: In this study we confirmed the value of a systemic administration of AdsFIt1-3 to block VEGF signaling as antitumor therapy in an experimental metastatic colorectal carcinoma model in mice. | Volker Schmitz Miroslaw Kornek Tobias Hilbert Christian Dzienisowicz Esbher Raskopf Christian Rabe Tilman Sauerbruch Cheng Qian Wolfgang H Caselmann | 2005 | World Journal of Gastroenterology2005，11，28： | 4 |
| 3 | Estimation of linear functionals on Sobolev spaces with applications to Fourier transforms and spline interpolation显示文摘 | Bramble J H Hilbert S R | 1974 | SIAM J Numer Anal1974，7，： | 2 |
| 4 | Noninvasive pressure support venti lation in COPD patients with postextubation hypercapnic respiratory insuffic iency显示文摘 | Hilbert G Gruson D Portel L | 1998 | Eur Resp J1998，11，： | 2 |
| 5 | The w:rld's technological capacity to store, com- municate, and eaxnpute information 显示文摘 | Hilbert M : P | 2011 | Science2011，332，： | 1 |
| 6 | Rotation and restricted use of antibiotics in medical intensive care unit显示文摘 | Gruson D Hilbert G Vargas F | 2000 | Am J Respit Crit Care Med2000，162，3： | 1 |
| 7 | Hypothesis:neoplasms in myotonic dystrophy显示文摘 | Mueller CM Hilbert JE Martens W | 2009 | Cancer Causes&Control2009，20，： | 1 |
| 8 | Obesity in print: an analysis of daily newspapers显示文摘 | HILBERT A RIED J | 2009 | Obes Facts2009，2，1： | 1 |
| 9 | Estimation of Linear Functionals on Sobolev Spaces with Application to Fourier Trans- forms and Spline Interpolation显示文摘 | BRAMBLE J H HILBERT S R | 1970 | SIAM J Numer Anal1970，7，1： | 1 |
| 10 | The effects of massage on delayed onset muscle soreness显示文摘 | Hilbert JE Sforzo GA Swenson T | 2003 | Br J Sports Med2003，37，4： | 1 |
| 11 | Bronchoscopy with bronehoalveolar lavage via the laryngeal mask airway in high risk hypoxemic immuno-suppressed patients显示文摘 | Hilbert G Gruson D Cardinaud JP | | 0，，02： | 1 |
| 12 | Thrombembolieprophylaxe auf deutschen Intensivstationen显示文摘 | Dr. P. Hilbert DEAA P. Teumer PD Dr. R. Stuttmann | 2008 | Der Anaesthesist2008，，3： | 1 |
| 13 | Chromosomal de-fects and outcome in 1 015 fetuses with increased nuchal trans-lucency显示文摘 | Pandya PP Kondylios A Hilbert L | 1995 | Ultrasound Obstet Gynecol1995，5，1： | 1 |
| 14 | A new and serious septicemic disease of young ducks with a description of the causative organism, Pfeifferella anatipestifer显示文摘 | HendricksonJ M Hilbert K F | 1932 | Cornell Vet1932，22，： | 1 |
| 15 | Resilience in landscape exploitation systems显示文摘 | Fletcher C Hilbert D W | 2007 | Ecological Modelling2007，201，44： | 1 |
| 16 | Effect of CO2 enrichmentand nitrogen availability on resource acquisition and resource alloca-tion in a grass,Bromus mollis显示文摘 | Larigauderie A Hilbert D W Oechel W C | 1988 | Oecologia1988，77，： | 1 |
| 17 | Never the tube! Try the mask! 显示文摘 | Hilbert G Vargas F Gruson D | 2007 | Crit Care Med2007，35，3： | 1 |
| 18 | Reflex sympa- thetic dystrophy in hemiplegia显示文摘 | Tepperman PS Greyson ND Hilbert L | 1984 | Archives of Physical Medi- cine and Rehabilitation1984，65，8： | 1 |
| 19 | The self-fulfilling influence of mother expectations on children's underage drinking显示文摘 | Madon S Guyll M Spoth R L Cross S E Hilbert S J | 2003 | Journal of Personality and Social Psychology2003，84，6： | 1 |
| 20 | Strategy of antibiotic rotation: long-term effect on incidence and SUS-ceptibilities of gram-negative bacili responsible for ventilator-associated pneumonia 显示文摘 | Gruson D Hilbert G Vargas F | 2003 | Crit Care Meal2003，31，7： | 1 |
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