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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | 显示文摘 | Li Y F He G F | 1998 | Synth Met1998，94，： | 2 |
| 3 | 3-D position sensitive CdZnTe gamma ray spectremeters显示文摘 | He Z Li W Knoll G F | 1999 | Nucl Instr and Meth in Phys Res1999，422，： | 1 |
| 4 | Binding of puerarin to human serum albumin:a spectroscopic analysis and molecular docking显示文摘 | HE Y WANG Y W TANG L F LIU H CHEN W ZHENG Z L ZOU G L | 2008 | J Fluoresc2008，18，2： | 1 |
| 5 | Inverse application of charge simulation method in detecting faulty ceramic insulators and processing influence from tower 显示文摘 | HE W YANG F WANG J G | 2006 | IEEE Transactions on Magnetics2006，42，4： | 1 |
| 6 | Newly synthesized dyes and their polymer/Glass composites for one-and two-photon pumped solid-state cavity lasing显示文摘 | Zhao C F He G S Bhawalkar J D | 1995 | Chem Mater1995，，7： | 1 |
| 7 | Sea surface temperature variability in the north side of the South China Sea over the last 55 years revealed by coral δ^18O records显示文摘 | Peng Z C He J F Chen T G | 2003 | Geochimica et Cosmochimica Acta2003，67，18： | 1 |
| 8 | Expression of estrogen receptors ERalpha and ERbeta in endometrial hyperplasia and adenocarcinoma 显示文摘 | Hu K Zhong G He F | 2005 | Int J Gynecol Cancer2005，15，6： | 1 |
| 9 | No Significant Association of 14 Candidate Genes With Schizophrenia in a Large European Ancestry Sample: Implications for Psychiatric Genetics显示文摘 | Sanders Alan R Duan Jubao Levinson Douglas F Shi Jianxin He Deli Hou Cuiping Burrell Gregory J Rice John P Nertney Deborah A Olincy Ann Rozic Pablo Vinogradov Sophia Buccola Nancy G Mowry Bryan J Freedman Robert Amin Farooq Black Donald W | 2008 | The American Journal of Psychiatry2008，，4： | 1 |
| 10 | Ultraviolet degradation of methyltins: Elucidating the mechanism by identification of a detected new intermediary product and investigating the kinetics at various environmental conditions 显示文摘 | Zhai G S Liu J F He B | 2008 | Chemosphere2008，72，3： | 1 |
| 11 | Percutaneous ethanol injection therapy in the treatment ofthyroid and parathyroid diseases显示文摘 | B E N N E D B A E K F N K A R S T R U P S HE G E D U S L | 1997 | Eur J Endocrinol1997，136，3： | 1 |
| 12 | Expression of estrogen receptors ERalpha and ERbeta in endometrial hyperplasia and adenocarcinoma显示文摘 | HU K ZHONG G HE F | 2005 | Int J Gynecol Cancer2005，15，： | 1 |
| 13 | Proton conductivity of phosphoric acid doped polybenzimidazole and its composites with inorganic proton conductors显示文摘 | HE R H LI Q F XIAO G | 2003 | Journal of Membrane Science2003，226，12： | 1 |
| 14 | Increased cadmium and lead uptake of a cadmium hyperaccumulator tomato by cadmium-resistant bacteria 显示文摘 | HE L Y CHEN A J REN G D ZHANG Y F QIAN M SHENG X F | 2009 | Ecotoxicology and Environmental Safety2009，72，： | 1 |
| 15 | Metabolomic analysis of theresponse of growing pigs to dietary L -arginine supplementation 显示文摘 | HE Q H KONG X F WU G Y | 2009 | Amino acids2009，37，： | 1 |
| 16 | Large eddy simulation of flame structure and combustion mode in a hydrogen fueled supersonic combustor显示文摘 | Huang Z W He G Q Qin F | 2015 | International Journal of Hydrogen Energy2015，40，31： | 1 |
| 17 | Highly flexible polyelectrolyte nanotu bes 显示文摘 | Ai S F Lu G He Q | 2003 | J Am Chem Soc2003，125，11： | 1 |
| 18 | O ptical limiting,effect in a two-photon alsorption dye doped solid matrix显示文摘 | He G S Bhawalkar J D Zhao C F Pmsad P N | 1995 | Appl Phys Lett1995，67，： | 1 |
| 19 | Major causes of death among men and women in China显示文摘 | HE J GU D F WU X G | 2005 | N Engl J Med2005，353，11： | 1 |
| 20 | Atomic oxygen diffusion on and desorption from amorphous silicate surfaces显示文摘 | He Jiao Jing Dapeng Vidali G F | 2014 | Chem Phys2014，16，1： | 1 |
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