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| 1 | Octreotide ameliorates gastric lesions in chronically mild stressed rats显示文摘AIM:To evaluate the effect of chronic mild stress(CMS) on the emergence of gastric ulcers and possible modulation by octreotide,a synthetic somatostatin analogue. METHODS:Adult male Wistar rats were subjected to nine different unpredictable random stress procedures for 21 d,a multifactorial interactional animal model for CMS.Octreotide was administered daily for 21 d at two dose levels(50 and 90μg/kg)before exposure to stress procedure.Macro-and microscopical assessments were made,in addition to quantification of plasma corticosterone and gastric mucosal inflammatory,oxidative stress, and apoptotic biomarkers. RESULTS:Exposure to CMS elevated plasma corticosterone(28.3±0.6μg/dL,P=0.002),an event that was accompanied by gastric lesions(6.4±0.16 mm,P=0.01) and confirmed histopathologically.Moreover,the insult elevated gastric mucosal lipid peroxides(13±0.5 nmol/g tissue,P=0.001),tumor necrosis factor-α(3008.6±78.18 pg/g tissue,P<0.001),prostaglandin E2(117.1 ±4.31 pg/g tissue,P=0.002),and caspase-3 activity (2.4±0.14 OD/mg protein,P=0.002).Conversely,CMS mitigated interleukin-10(627.9±12.82 pg/g tissue,P= 0.001).Furthermore,in animals exposed to CMS,octreotide restored plasma corticosterone(61%and 71%from CMS,P=0.002)at both dose levels.These beneficial effects were associated with a remarkable suppression of gastric lesions(38%and 9%from CMS,P=0.01)and reversal of derangements in gastric mucosa. CONCLUSION:The current investigation provides evidence that exposure to CMS induces gastric ulceration, which was alleviated by administration of octreotide possibly possessing antioxidant,anti-inflammatory,and anti-apoptotic actions. | Noha N Nassar Mona F Schaalan Hala F Zaki Dalaal M Abdallah | 2011 | World Journal of Gastroenterology2011，17，9： | 6 |
| 2 | Molecular analysis and anticancer properties of two identified isolates,Fusarium solani and Emericella nidulans isolated from Wady El-Natron soil in Egypt against Caco-2(ATCC) cell line显示文摘Objective:To characterize,identify and investigate the anticancer properties of two new soil fungal isolates,Emericella nidulansand Fusarium solani isolated from Wady El-Natron in Egypt against colon cancer Caco-2(ATCCj cell line.Methods:Soil sample was cultured and two strains were chosen for morphological and phenotypical characterization.Partial sequences of the 18s rRNA gene and the internal transcribed spacer region ITS of the two isolates were amplified by PCR.Phylogenetic tree construction and analysis of the resulted multiple sequences from the two fugal isolates were also carried out.In vitro anticancer activity of the two strains was done against colon Caco-2 cancer cell line.Reverse transcription — PCR was carried out to detect level of expression of p53 in Caco-2 cell line.Results:HF.I displayed morphological and genotypic characteristics most similar to that of Fusarium solani while HF.2 was most similar to Emericella nidulans with high similarity of 99%and 97%respectively.The multiple sequence alignment of the two fungal isolates showed that,the maximum identical conserved domains in the 18s rRNA genes were identified with the nucleotide regions of Slst to 399th base pairs,88th to 525th base pairs respectively.While those in the ITS genes were identified with the nucleotide regions of 88th to 463rd and Slst to 274th.The two isolates showed IC<sub>50 value with(6.24±5.21) and(9.84±0.36) μ g/mL) concentrations respectively at 28h.Reverse transcription- PCR indicated that these cells showed high level of expression for p53 mRNA.Conclusions:The morphology and molecular analysis identified HF.1 and HF.2 to be Fusarium solani and Emericella nidulans;new isolates of anticancer producing fungi from Wady El-Natroon city in Egypt.Treatment with the two isolates caused P53 expression in Caco-2 cell line.These two isolates can be used as an anticancer agents. | Hala F Mohamed | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，11： | 3 |
| 3 | Drainage vs. non-drainage after cholecystectomy for acute cholecystitis:a retrospective study显示文摘Many surgeons practice prophylactic drainage after cholecystectomy without reliable evidence,this study was conducted to answer the question whether to drain or not to drain after cholecystectomy for acute calculous cholecystitis.A retrospective review of all patients who had cholecystectomy for acute cholecystitis in Aseer Central Hospital,Abha,Saudi Arabia,was conducted from April 2010 to April 2012.Data were extracted from hospital case files.Preoperative data included clinical presentation,routine investigations and liver function tests.Operative data included excessive adhesions,bleeding,bile leak,and drain insertion.Complicated cases such as pericholecystic collections,mucocele and empyema were also reported.Patients who needed therapeutic drainage were excluded.Postoperative data included hospital stay,volume of drained fluid,time of drain removal,and drain site problems.The study included 103 patients allocated into two groups;group A(n = 38) for patients with operative drain insertion and group B(n = 65) for patients without drain insertion.The number of patients with preoperative diagnosis of acute non-complicated cholecystitis was significantly greater in group B(80%) than group A(36.8%)(P < 0.001).Operative time was significantly longer in group A.All patients who were converted from laparoscopic to open cholecystectomy were in group A.Multivariate analysis revealed that hospital stay was significantly(P < 0.001) longer in patients with preoperative complications.There was no added benefit for prophylactic drain insertion after cholecystectomy for acute calculous cholecystitis in non-complicated or in complicated cases. | Mohammed A Bawahab Walid M Abd El Maksoud Saeed A Alsareii Fahad S Al Amri Hala F Ali Abdul Rahman Nimeri Abdul Rahman M Al Amri Adel A Assiri Mohammed I Abdul Aziz | 2014 | The Journal of Biomedical Research2014，28，3： | 3 |
| 4 | Chronic ocular GVHD:limbal and conjunctival stem cell allografts from the same hematopoietic stem cell donor显示文摘Dear Editor,I am Dr.Elias F Jarade from the Beirut Eye and ENT Specialist Hospital, Beirut, Lebanon. I write to describe a novel surgical technique in the management of chronic ocular graft-versus-host disease(GVHD).Allogeneic hematopoietic stem cell transplantation(HSCT) has become the standard of care in many | Elias F Jarade Hala El Rami Youssef Abdelmassih Mazen Amro | 2018 | International Journal of Ophthalmology(English edition)2018，11，9： | 2 |
| 5 | A rapid-curing alginate gel system:utility in periosteum-derived cartilage tissue engineering显示文摘 | Molly M S Hala F Q Robert L | 2004 | Biomaterials2004，25，： | 1 |
| 6 | Di- etary supplementation with benzoic acid improves ap- parent ileal digestibility of total nitrogen and increases villous height and caecal microbial diversity in weaner pigs 显示文摘 | HALAS D HANSEN C F HAMPSON D J | 2010 | Animal Feed Science and Technology2010，160，34： | 1 |
| 7 | Chitosan based nanofibers, review 显示文摘 | MAHER Z E HALA F N RANIA E M | 2012 | Materials Science and Engineering: C2012，32，7： | 1 |
| 8 | Effect of dietary supplementation with inulin and/or benzoic acid on the incidence and severity of post- weaning diarrhoea in weaner pigs after experimental challenge with enterotoxigenic Escherichia coli 显示文摘 | HALAS D HANSEN C F HAMPSON D J | 2009 | Archives of Animal Nutrition2009，63，4： | 1 |
| 9 | Treatment of domestic wastewater using an anaerobic baffled reactor followed by a duckweed pond for agricultural purposes显示文摘 | Fayza A Nasr Hala S Donna Hossaam F Nassar | 2009 | Environmentalist2009，29，： | 1 |
| 10 | Giant peritoneal loose body in the pelvic ('avity : repo,l of a ease显示文摘 | Nomura H Hala F Yasoshima T e! al | 2003 | Surg Today2003，33，10： | 1 |
| 11 | The effects of weaning age,diet composition,and categorisation of creep feed intake by piglets on diarrhoea and performance after weaning显示文摘 | Callesen J Halas D Thorup F | 2007 | Livestock Science2007，108，13： | 1 |
| 12 | Chitosan based nanofibers,review 显示文摘 | Maher Z E Hala F N Rania E M | 2012 | Materials Science and Engineering C2012，32，： | 1 |
| 13 | Phase I clinical study of anti-apoptosis protein, survivin-derived peptide vaccine thera-P for patients with advanced or recurrent colorectal cancer显示文摘 | TSURUMA T HALA F TORIGOE T | 2004 | J Transl Med2004，2，1： | 1 |
| 14 | Geometrical parameters controlling sensitivity of nanoshell plasmon resonances to changes in dielectric environment 显示文摘 | Tam F Moran C Halas N J | 2004 | Journal of Physical Chemistry B2004，108，17： | 1 |
| 15 | Geometrical parameters controlling sensitivity of nanoshell plasmon resonances to changes in dielectric environment显示文摘 | TAM F MORAN C HALAS N | 2004 | The Journal of Physical Chemistry B2004，108，17： | 1 |
| 16 | Peptide-assembled optically responsive nanoparticle complexes显示文摘 | Slocik J M Tam F Halas N J | 2007 | Nano Letters2007，7，4： | 1 |
| 17 | Sol-gel synthesis of vanadium pentoxide nanoparticles in air-and water-stable ionic liquids 显示文摘 | MOHAMMAD A Z HALA K F | 2009 | Journal of Materials Science Letters2009，44，： | 1 |
| 18 | 查看详情显示文摘 | Knight M W Grady N K Bardhan R Hao F Nordlander E Halas N J | | 0，，： | 1 |
| 19 | Characterization of Amphidinium(Amphidiniales,Dinophyceae)species from the China Sea based on morphological,molecular,and pigment data显示文摘Amphidinium species are amongst the most abundant benthic dinoflagellates in marine intertidal sandy ecosystems.Some of them are able to produce a variety of bioactive compounds that can have both harmful effects and pharmaceutical potentials.The diversity of Amphidinium in shallow waters along the Chinese coast was investigated by isolating single cells from sand,coral,and macroalgal samples collected from 2012 to 2020.Their morphologies were subjected to examination using light microscopy(LM)and scanning electron microscopy(SEM).A total of 74 Amphidinium strains were morphologically identified,belonging to 11 species:A.carterae,A.gibbosum,A.operculatum,A.massartii,A.cf.massartii,A.fijiensis,A.pseudomassartii,A.steinii,A.thermaeum,A.theodori,A.tomasii,as well as an undefined species.The last seven species have not been previously reported in Chinese waters.Amphidinium carterae subcladesⅠ,Ⅱ,andⅣwere found in the South China Sea,while subcladeⅢwas only found in the Yellow Sea.Threadlike body scales were observed on the surface of subcladesⅢ and Ⅳ,supporting the idea that A.carterae might contain several different species.Large subunit ribosomal RNA(LSU rRNA)sequencesbased phylogeny revealed two groups(GroupsⅠandⅡ)within Amphidinium,which is consistent with the relative position of sulcus(in touch with cingulum or not).In addition,large differences in morphology and molecular phylogeny between A.operculatum(the type species of Amphidinium)and other species,suggest that a subdivision of Amphidinium might be needed.The pigment profiles of all available strains were analyzed by high performance liquid chromatography(HPLC).Eleven pigments,including peridinin,diadinoxanthin,diatoxanthin,pheophorbide(and pheophorbide a),antheraxanthin,β-carotene,and four different chlorophylls were detected.The high pheophorbide/pheophorbide a ratio in Amphidinium implies that it may be a good candidate as a natural source of photosensitizers,a well-known anticancer drug. | Zhaohe LUO Hua ZHANG Qun LI Lei WANG Hala F MOHAMED Songhui LÜ Haifeng GU | 2022 | Journal of Oceanology and Limnology2022，40，3： | 1 |
| 20 | Di- etary supplementation with benzoic acid improves ap- parent ileal digestibility of total nitrogen and increases villous height and caecal microbial diversity in weaner pigs 显示文摘 | HALAS D HANSEN C F HAMPSON D J | 2010 | Animal Feed Science and Technology2010，160，： | 1 |
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