维普期刊资源整合服务平台（V6.5）

24篇 您的检索式：作者名="Gyetko"

选中清除导出查看参考文献查看引证文献引用追踪共2页首页上一页第1页下一页末页 /2 跳转

	题名	作者	年代	出处	被引量
1	Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection 显示文摘	Gyetko MR Sud S Kendall T	2000	J Immunol2000，165，3：	1
2	New insights in- to the pathogenesis and treatment of idiopathic pulmonary fibrosis : apotential role for stem ceils in the lung parenchyma and implica- tions for therapy显示文摘	Gharaee - Kermani M Gyetko MR Hu B	2013	Pharm Res2013，24，5：	1
3	Enzyme-linked immunoabs orbentassay detection of as oluble form of urokinase plasminogen activator receptor in vivo显示文摘	Mizukami IF Faulkner NE Gyetko MR	1995	Blood1995，86，：	1
4	Antigen-driven lymphocyte recruitment to the lung is diminished in the absence of urokinase-type plasminogen activator ( uP A ) receptor, but is independent of uP A显示文摘	Gyetko M R Sud S J Sonstein J	2001	Llmmunol2001，167，10：	1
5	New Insights into the Pathogenesis and Treatment of Idiopathic Pulmonary Fibrosis: A Potential Role for Stem Cells in the Lung Parenchyma and Implications for Therapy显示文摘	Mehrnaz Gharaee-Kermani Margaret R. Gyetko Biao Hu Sem H. Phan	2007	Pharmaceutical Research2007，，5：	1
6	Expression of heterogeneous profiles of plasminogen activators and plasminogen activator inhibitors by human glioma lines显示文摘	Sitrin RG Gyetko MR Kole KL	1990	Cancer Res1990，50，16：	1
7	New insights into the pathogenesis and treatment of idiopathic pulmonary fibrosis:a potential role for stem cells in the lung parenchyma and implications for therapy显示文摘	Gharaee-Kermani M Gyetko MR Hu B		0，，5：	1
8	Enzyme - linked immunoabsorbent assay detection of a soluble form of urokinase plasminogen activator receptor in vivo 显示文摘	Mizukami IF Faulkner NE Gyetko MR	1995	Blood1995，86，1：	1
9	慢性阻塞性肺疾病患者支气管肺泡灌洗液细菌宏基因组DNA的制备显示文摘【目的】优化稳定期慢性阻塞性肺疾病(chronic obstructive pulmonary diseases,COPD)患者支气管肺泡灌洗液(bronchoalveolar lavage fluids,BALF)细菌宏基因组DNA的提取方法,以便于高效提取微量的细菌DNA进行后续的PCR反应和测序。【方法】取稳定期COPD患者的BALF 5mL,离心收集细胞。为了有效提取样品中革兰氏阳性菌的基因组,对QIAGEN的DNA提取试剂盒的操作步骤进行优化:加入裂解缓冲液ATL后首先运用研磨珠和多功能生物样品匀质器破碎菌壁,再加入蛋白酶K孵育,然后加入裂解缓冲液AL振荡混匀。无水乙醇沉淀DNA后,将全部溶液过柱,用洗液AW1和AW2各洗柱一次,最后加50μL洗脱液洗脱DNA。提取的DNA定量后,运用PCR方法检测样本中的细菌16S rDNA量,并按照测序要求构建DNA文库进一步验证。【结果】试剂盒优化法提取的BALF的DNA总量为467.5(135.0-1697.5)ng,明显高于按照传统酚-氯仿法提取的DNA总量95.0(0-612.5)ng,并且所提取的DNA可以很好的扩增细菌的16S rDNA以及构建DNA文库,改良后的扩增产物明显增多(P=0.002)。【结论】使用DNA提取试剂盒结合研磨珠和多功能生物样品匀质器破菌壁的方法能够更高效的提取BALF中微量的宏基因组DNA,为进一步的测序和菌群分析打下基础。	王娟沈宁杜毅鹏 John R Erb-downward Gary B Huffnagle Margaret R Gyetko 贺蓓	2014	微生物学报2014，54，8：	1
10	Urokinasetype plasminogen activator potentiates lipopolysac charide-induced neutrophil activation 显示文摘	Abraham E Gyetko MR Kuhn K	2003	J Immunol2003，170，11：	1
11	Urokinase-type plasminogen activator potentiates lipopolysaccharide-induced neutrophil activation 显示文摘	Abraham E Gyetko MR Kuhn K	2003	J Immunol2003，170，：	1
12	Urokinase-type plasminogen activator potentiates lipopolysaccharide- induced neutrophil activation显示文摘	Abraham E Gyetko MR Kuhn K	2003	J Immunol2003，170，11：	1
13	Urokinase is required for the pulmonary inflammation response to Cryptococcus netoformans 显示文摘	Gyetko M R Chen G H McDonald R A	1996	J Clin Invest1996，98，8：	1
14	Enzyme-liked immyunoabaorbert assay detection of a soluble form of urokinase plasminogen activator receptor in vivo 显示文摘	Mizuami I F Faulkner N E Gyetko M R	1995	Blood1995，86，1：	1
15	Pericellular proteolysis by leukocytes and tumor cells on substrates: focal activation and the role of urokinase-type plasminogen activator显示文摘	Andrei L. Kindzelskii Imran Amhad Donald Keller M.-J. Zhou Richard P. Haugland B. A. Garni-Wagner Margaret R. Gyetko Robert F. Todd Howard R. Petty	2004	Histochemistry and Cell Biology2004，，4：	1
16	Urokinade is required for the pulmonary inflammatory response to Cryptococcus neofornans,A murine transgenic model显示文摘	GYETKO MR CHEN GH MCDONALD RA	1996	J Clin Invest1996，97，8：	1
17	Urokinase-type plasminogen activator in inflammatory cell recruitment and host defense against pneumocystis carinii in mice显示文摘	Beck JM Preston Am Gyetko MR	1999	Infect immun1999，67，：	1
18	New insights into the pathogenesis and treatment of idiopathic pulmonary fibrosis: a potential role for stem cells in the lung parenchyma and implications for therapy显示文摘	Gharaee-Kermani M Gyetko MR Hu B	2007	Pharm Res2007，24，5：	1
19	Urokinade is required for the pulmonary inflammatory response to Cryptocoecus neofornans, A murine transgenic model显示文摘	GYETKO MR CHEN CH MCDONALD RA	1996	J Clin Invest1996，97，8：	1
20	Antigen-driven lymphocyte recruitment to the lung is diminished in the absence of urokinase-type plasminogen activator (uPA) receptor, hut is independent of uPA显示文摘	Gyetko MR Sud S Sonstein J	2001	J Immunol2001，167，10：	1

返回顶部每页显示：

共2页首页上一页第1页下一页末页 /2 跳转

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

用户提示