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| 1 | 环丙沙星对培养的铜绿假单孢菌释放DNA的影响显示文摘目的观察不同浓度的环丙沙星对培养的铜绿假单孢菌释放DNA的影响.方法选择体外对铜绿假单孢菌1244株(ATCC 27317)敏感的环丙沙星为实验所用的抗生素, 检测其最小最小抑菌浓度(MIC)和最小杀菌浓度(MBC);将不同稀释度(32~0.03 mg/L)的环丙沙星与该菌株菌液在37℃培养,分别取其培养4 h和24 h的上清液经DNA电泳法检测其细菌DNA释放的情况.结果无论有无环丙沙星,DNA电泳法均未见到培养4 h 的样本存在细菌释放的游离DNA.体外培养24 h,铜绿假单孢菌可自发释放一定量的游离DNA分子,呈两个明显的区带, 其分子量分别大于2000 bp和小于100 bp;远低于MIC的环丙沙星可诱导铜绿假单孢菌释放更多的游离DNA, 分布在3个明显的区带,其中两个区带大于2000 bp,另1个区带小于100 bp;大于或等于MIC的环丙沙星对铜绿假单孢菌无诱导DNA释放的作用.结论自然生长情况下铜绿假单孢菌就可自发释放一定量的游离DNA分子;低于MIC的环丙沙星存在使这一现象更加明显,而且铜绿假单孢菌释放游离DNA分子的多少和分子量大小均有明显的差别. | 彭代智 Charles H. Guymon Albert T. McManus 肖光夏 | 2005 | 中华外科杂志2005，43，3： | 3 |
| 2 | The formation of higher aliphatic alco- hols by mutant strains of Saccharomyces cerevisia显示文摘 | INGRAHAM J L GUYMON J F | 1960 | Arch Bioehem Biophys1960，88，： | 1 |
| 3 | Photopolymerizationkinetics and structure development of templated lyotropic liquidcrystalline systems显示文摘 | LESTER C L COLSON C D GUYMON C A | 2001 | Macromolecules2001，34，13： | 1 |
| 4 | Acceleration ofpolyacrylamide photopolymerization using lyotropic liquid crystaltemplates显示文摘 | LESTER C L SMITH S M GUYMON C A | 2001 | Macromolecules2001，34，： | 1 |
| 5 | Formation of higher alcohols during grape juice fermentations at various temperatures显示文摘 | OUGH C S GUYMON J F CROWELL E A | 1966 | J Food Sci1966，31，： | 1 |
| 6 | The formation of n-propyl alcohol by Saccharomyces cerevisiae显示文摘 | GUYMON J F INGRAHAM J L Crowell E A | 1961 | Arch Biochem Bio- phys1961，95，： | 1 |
| 7 | 查看详情显示文摘 | White T J Liechty W B Natarajan L V Tondiglia V P Bunning T J Guymon C A | | 0，，： | 1 |
| 8 | A detailed view of a ribosomal active site: the structure of the LII-RNA complex 显示文摘 | Wimberly BT Guymon R McCuteheon JP | 1999 | Cell1999，97，4： | 1 |
| 9 | A one-dimensional frost heave model based upon simulation of simultaneous heat and water flux显示文摘 | Guymon G Berg R Hromadka T | 1980 | Cold Regions Science and Technology1980，8，23： | 1 |
| 10 | Miniature cylindrical ion trap mass spec- trometer显示文摘 | PATTERSON G E GUYMON A J RITER L S | 2002 | Analytical Chemistry2002，74，24： | 1 |
| 11 | Miniature cylindrical ion trap mass spectrometer 显示文摘 | Patterson G E Guymon A J Riter L S | 2002 | Analytical Chemistry2002，74，24： | 1 |
| 12 | A detailed view of a ribosomal active site: the structure of the L11-RNA complex 显示文摘 | Wimberly B T Guymon R McCutcheon J P | 1999 | Cell1999，97，4： | 1 |
| 13 | Chemical compatibility and reaction-induced exfoliation in photopolymerizable clay nanocomposites显示文摘 | Owusu-Adom K Guymon C A | 2009 | Macromolecules( S1598-5032)2009，42，1： | 1 |
| 14 | Two-dimensional model of coupled heat and moisture transport in frost-heaving soils显示文摘 | GUYMON G L HROMADKA T T BERY R L | 1984 | Energy Resource Technology Trans of ASME1984，106，： | 1 |
| 15 | Miniature cylindrical ion trap mass spectrometer显示文摘 | PATTERSON G E GUYMON A J RITER L S | 2002 | Anal Chem2002，74，24： | 1 |
| 16 | One Dimensional Frost Heaven Model Based upon Simulation of Simultaneous Heat and Water Flux显示文摘 | GUYMON G BERG R HROMADKA T A | 1980 | Cold Regions Science and Technology1980，3，3： | 1 |
| 17 | The role of SPS, MPSA, and chloride in additive systems for copper electrodeposition 显示文摘 | TAN M GUYMON C WHEELER D R | 2007 | Journal of the Electrochemical Society2007，154，2： | 1 |
| 18 | The alar base cinch suture to control nasal width in maxillary osteotomies 显示文摘 | Guymon M Crosby D Wolford LM | 1988 | Int J Adult Orthod Orthogn Surg1988，3，2： | 1 |
| 19 | A one-dimensional frost heaven model based upon simulation of simultaneous heat and water flux显示文摘 | GUYMON G BERG R HROMADKA T | 1980 | Cold Regions Science and Technology1980，3，： | 1 |
| 20 | Changes in Some Volatile Constituents of Brandy During Aging 显示文摘 | Masami O Guymon J F Crowell E A | 1977 | American journal of Enology and Viticulture1977，28，3： | 1 |
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