|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 2 | Intravitreal triameinolone acetonide in the treatment of macular edema clue to retinal vein occlusion 显示文摘 | Guo MX Yi CX Xiang DM | 2010 | Int Eye Sci2010，10，6： | 1 |
| 3 | Genetic basis of thoracic aortic aneurysms and dissections:focus on smooth muscle cell contractile dysfunction显示文摘 | Milewicz DM Guo DC Tran-Fadulu V | 2008 | Annu Rev Genomics Hum Genet2008，9，： | 1 |
| 4 | In vitro eytotoxieity, pharmacokinetics, tissue distribution, and metabolism of small- molecule protein kinase D inhibitors, kb-NB142-70 and kb- NB165-09, in mice bearing human cancer xenografts显示文摘 | Guo J Clansen DM Beumer JH | 2013 | Cancer Chemother Pharmacol2013，71，2： | 1 |
| 5 | Genetic basis of thoracic aortic aneurysms and dissections: focus on smooth muscle cell contractile dysfunction显示文摘 | Milewicz DM Guo DC Tran-Fadulu V | 2008 | Annu Rev Genomics Hum Genet2008，9，： | 1 |
| 6 | Iso-parametric toolpath generation from triangular meshes for free-formsurface machining 显示文摘 | Sun YW Guo DM Jia ZY Wang HX | 2006 | Int J Adv Manuf Technol2006，28，： | 1 |
| 7 | Calcium signaling in cardiac ventricular myocytes显示文摘 | Bers DM Guo T | 2005 | Ann N Y Acad Sci2005，，1047： | 1 |
| 8 | Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae 显示文摘 | Yang S Land ML Klingeman DM Pelletier DA Lu TY Martin SL Guo HB Smith JC Brown SD | 2010 | Proc Natl Acad Sci USA2010，107，10： | 1 |
| 9 | HIV epidemic among drug users in China: 1995 -2011 显示文摘 | Wang L Guo W Li DM | 2015 | Addiction2015，110，1： | 1 |
| 10 | Modulation of p53, ErbB1, ErbB2, and Raf-1 expression in lung cancer cells by depsipeptide FR901228显示文摘 | Yu X Guo ZS Marcu MG Neckers L Nguyen DM Chen GA | 2002 | J Natl Cancer Inst2002，94，7： | 1 |
| 11 | Genetic basis of thoracic aortic aneurysms and dissections 显示文摘 | Hasham SN Guo DC Milewicz DM | 2002 | Curt Opin Cardiol2002，17，6： | 1 |
| 12 | Properties of halloysite nanotube - epoxy resin hybrids and the interfacial reactions in the systems 显示文摘 | Liu MX Guo BC Du ML Cai XJ Jia DM | 2007 | Nanotechnology2007，18，45： | 1 |
| 13 | Effects of epoxy content on dynamic me- ehanical behaviour of PEI - toughened dieyanate - novolac epoxy blends显示文摘 | Guo BC Jia DM | 2004 | Polymer International2004，53，9： | 1 |
| 14 | A level set method for structural topology optimization 显示文摘 | Wang M Wang XM Guo DM | 2003 | Computer Methods in Applied Mechanics and Engineering (S0045-7825)2003，192，12： | 1 |
| 15 | The type Ⅳ pilin of Burkholderia mallei is highly immunogenic but fails to protect against lethal aerosol challenge in a murine model显示文摘 | Fernandes PJ Guo Q Waag DM | 2007 | Infect Immun2007，75，6： | 1 |
| 16 | Modulation ofp53,ErbB1,ErbB2,and Raf-1 expression in lung cancer cells by depsipeptide FR901228显示文摘 | Yu X Guo ZS Marcu MG Neckers L Nguyen DM Chen GA | 2002 | JNatl Cancer Inst2002，94，： | 1 |
| 17 | Oxidation of spin trap 5,5-dimethyl-1-pyrroline-1-oxide in an electron paramagnetic resonance study of thereaction of methemoglobin with hydrogen peroxide显示文摘 | Guo DM Panakezhum D Mark J | 1994 | Free Radio Biol Med1994，16，： | 1 |
| 18 | Preparation and Application of a new Curing Agent for Epoxy Resin 显示文摘 | Zhang JH Qiu QH Yek Yeat Wang FD Jia ZX Guo BC Jia DM | 2012 | International Journal of Poly- meric Materials2012，61，7： | 1 |
| 19 | Calcium Signaling in Cardiac Ventricular Myocytes显示文摘 | Bers DM Guo T | 2005 | Ann N Y Acad Sci2005，1047，1： | 1 |
| 20 | Generation of meat-like flavourings from enzymatic hydro|ysates of proteins from Brassica sp显示文摘 | Guo XF Tian S J Small DM | 2010 | Food Chemistry2010，119，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员