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| 1 | Non-invasive assessment of barrier integrity and function of the human gut显示文摘Over the past decades evidence has been accumulating that intestinal barrier integrity loss plays a key role in the development and perpetuation of a variety of disease states including inflammatory bowel disease and celiac disease,and is a key player in the onset of sepsis and multiple organ failure in situations of intestinal hypoperfusion,including trauma and major surgery.Insight into gut barrier integrity and function loss is important to improve our knowledge on disease etiology and pathophysiology and contributes to early detection and/or secondary prevention of disease.A variety of tests have been developed to assess intestinal epithelial cell damage,intestinal tight junction status and consequences of intestinal barrier integrity loss,i.e.increased intestinal permeability.This review discusses currently available methods for evaluating loss of human intestinal barrier integrity and function. | Joep Grootjans Geertje Thuijls Froukje Verdam Joep PM Derikx Kaatje Lenaerts Wim A Buurman | 2010 | World Journal of Gastrointestinal Surgery2010，2，3： | 18 |
| 2 | Life and death at the mucosal-luminal interface: New perspectives on human intestinal ischemia-reperfusion显示文摘Intestinal ischemia is a frequently observed phenomenon. Morbidity and mortality rates are extraordinarily high and did not improve over the past decades. This is in part attributable to limited knowledge on the pathophysiology of intestinal ischemia-reperfusion(IR) in man, the paucity in preventive and/or therapeutic options and the lack of early diagnostic markers for intestinal ischemia. To improve our knowledge and solve clinically important questions regarding intestinal IR, we developed a human experimental intestinal IR model. With this model, we were able to gain insight into the mechanisms that allow the human gut to withstand short periods of IR without the development of severe inflammatory responses. The purpose of this review is to overview the most relevant recent advances in our understanding of the pathophysiology of human intestinal IR, as well as the(potential) future clinical implications. | Joep Grootjans Kaatje Lenaerts Wim A Buurman Cornelis HC Dejong Joep PM Derikx | 2016 | World Journal of Gastroenterology2016，22，9： | 11 |
| 3 | Human small intestine is capable of restoring barrier function after short ischemic periods显示文摘AIM To assess intestinal barrier function during human intestinal ischemia and reperfusion(IR).METHODS In a human experimental model,6 cm of jejunum was selectively exposed to 30 min of ischemia(I) followed by 30 and 120 min of reperfusion(R). A sham procedure was also performed. Blood and tissue was sampled at all-time points. Functional barrier function was assessed using dual-sugar absorption tests with lactulose(L) and rhamnose(R). Plasma concentrations of citrulline,an amino acid described as marker for enterocyte function were measured as marker of metabolic enterocytes restoration. Damage to the epithelial lining was assessed by immunohistochemistry for tight junctions( TJs),by plasma marker for enterocytes damage(I-FABP) and analyzed by electron microscopy(EM) using lanthanum nitrate as an electrondense marker.RESULTS Plasma L/R ratio's were significantly increased after 30 min of ischemia(30 I) followed by 30 min of reperfusion(30 R) compared to control(0.75 ± 0.10 vs 0.20 ± 0.09,P < 0.05). At 120 min of reperfusion(120 R),ratio's normalized(0.17 ± 0.06) and were not significantly different from control. Plasma levels of I-FABP correlated with plasma L/R ratios measured at the same time points(correlation: 0.467,P < 0.01). TJs staining shows distortion of staining at 30 I. An intact lining of TJs was again observed at 30 I120 R. Electron microscopy analysis revealed disrupted TJs after 30 I with paracellular leakage of lanthanum nitrate,which restored after 30 I120 R. Furthermore,citrulline concentrations closely paralleled the histological perturbations during intestinal IR.CONCLUSION This study directly correlates histological data with intestinal permeability tests,revealing that the human gut has the ability of to withstand short episodes of ischemia,with morphological and functional recovery of the intestinal barrier within 120 min of reperfusion. | Dirk HSM Schellekens Inca HR Hundscheid Claire AJI Leenarts Joep Grootjans Kaatje Lenaerts Wim A Buurman Cornelis HC Dejong Joep PM Derikx | 2017 | World Journal of Gastroenterology2017，23，48： | 4 |
| 4 | A Pilot Study on the Noninvasive Evaluation of Intestinal Damage in Celiac Disease Using I-FABP and L-FABP显示文摘 | Joep P. M. Derikx Anita C. E. Vreugdenhil Anita M. Van den Neucker Joep Grootjans Annemarie A. van Bijnen Jan G. M. C. Damoiseaux L. W. Ernest van Heurn Erik Heineman Wim A. Buurman | 2009 | Journal of Clinical Gastroenterology2009，，8： | 2 |
| 5 | Level of activation of the unfolded protein response correlates with Paneth cell apoptosis in human small intestine exposed to ischemia/reperfusion 显示文摘 | Grootjans J Hodin CM Haan J J | 2011 | Gastroen- terology2011，140，2： | 1 |
| 6 | Dynamics of seasonal bicarbonate supply in a dune slack:Effects on organic matter,nitrogen pool and vege- tation succession显示文摘 | Sival EP Grootjans AP | 1996 | Vegetatio1996，126，： | 1 |
| 7 | Determina- tion of apoptotic and necrotic cell death in vitro and in vivo显示文摘 | Vanden Berghe T Grootjans S Goossens V | 2013 | Methods2013，61，2： | 1 |
| 8 | Hydro-geochemical analysis of a degraded dune slack 显示文摘 | Grootjans AP Sival EP Stuyfzand PJ | 1996 | Vegetatio1996，126，： | 1 |
| 9 | Prospects of fen restoration in relation to changing land use--an exam- ple from central poland显示文摘 | Agata K Paulina D Grootjans A P | 2010 | Landscape and Urban Plan- ning2010，97，4： | 1 |
| 10 | Syntenin-syn-decanbinding requires syndecan-synteny and the co-operation ofboth PDZ domains of syntenin 显示文摘 | Grootjans JJ Reekmans G Ceulemans H | 2000 | J Biol Chem2000，275，19： | 1 |
| 11 | Restoration of wet dune sla- cks on the Dutch Wadden Sea islands : Recolonization after large- scale sod cutting显示文摘 | Grootjans AP Everts H Bruin K | 2001 | Restoration Ecology2001，9，2： | 1 |
| 12 | Groundwater variables and vegetation in dune slacks显示文摘 | Lammerts E J Maas C Grootjans AP | 2001 | Ecological Engineering2001，17，： | 1 |
| 13 | How to define targets for ecological restoration:introduction显示文摘 | Bakker J P Grootjans A P Hermy M | | 0，，01： | 1 |
| 14 | Plasma intestinal fatty acid-binding protein levels correlate with morphologic epithe- lial intestinal damage in a human translational ischemia-reperfu- sion model 显示文摘 | Schellekens DH Grootjans JM Dello SA | 2014 | J Clin Gastroentero12014，48，3： | 1 |
| 15 | The state of the art of aquatic and semi-aquatic ecological restoration projects in the Netherlands显示文摘 | Nienhuis P H Bakker J P Grootjans A P | 2002 | Hydrobiologia2002，478，15： | 1 |
| 16 | Starvation compromises Paneth ceils 显示文摘 | Hodin CM Lenaerts K Grootjans J | 2011 | Am J Pathol2011，179，： | 1 |
| 17 | Human intestinal ische- mia-reperfusion-induced inflammation characterized: experiences from a new translational model 显示文摘 | Grootjans J Lenaerts K Derikx JP | 2010 | AmJ Pathol2010，176，5： | 1 |
| 18 | How to define targets for ecological restoration?显示文摘 | BakkorJP Grootjans A P Hermy M | 2000 | Applied Vegetation Science2000，3，： | 1 |
| 19 | Human intesti- nal ischemia-reperfusion-induced inflammation character- ized: experiences from a new translational model显示文摘 | Grootjans J Lenaerts K Derikx JP ct al | 2010 | Am J Pathol2010，176，5： | 1 |
| 20 | Goblet cell compound exocytosis in the defense against bacterial in- vasion in the colon exposed to ischemia-reperfusion 显示文摘 | Grootjans J Hundscheid IH Buurman WA | 2013 | Gut Microbes2013，4，3： | 1 |
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