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| 1 | A single-cell transcriptome of mesenchymal stromal cells to fabricate bioactive hydroxyapatite materials for bone regeneration显示文摘The osteogenic microenvironment of bone-repairing materials plays a key role in accelerating bone regeneration but remains incompletely defined,which significantly limits the application of such bioactive materials.Here,the transcriptional landscapes of different osteogenic microenvironments,including three-dimensional(3D)hydroxyapatite(HA)scaffolds and osteogenic medium(OM),for mesenchymal stromal cells(MSCs)in vitro were mapped at single-cell resolution.Our findings suggested that an osteogenic process reminiscent of endochondral ossification occurred in HA scaffolds through sequential activation of osteogenic-related signaling pathways,along with inflammation and angiogenesis,but inhibition of adipogenesis and fibrosis.Moreover,we revealed the mechanism during OM-mediated osteogenesis involves the ZBTB16 and WNT signaling pathways.Heterogeneity of MSCs was also demonstrated.In vitro ossification of LRRC75A+MSCs was shown to have better utilization of WNT-related ossification process,and PCDH10+MSCs with superiority in hydroxyapatite-related osteogenic process.These findings provided further understanding of the cellular activity modulated by OM conditions and HA scaffolds,providing new insights for the improvement of osteogenic biomaterials.This atlas provides a blueprint for research on MSC heterogeneity and the osteogenic microenvironment of HA scaffolds and a database reference for the application of bioactive materials for bone regeneration. | Peng Guo Xizhe Liu Penghui Zhang Zhongyuan He Zhen Li Mauro Alini RGeoff Richards Sibylle Grad Martin J.Stoddart Guangqian Zhou Xuenong Zou Danny Chan Wei Tian Dafu Chen Manman Gao Zhiyu Zhou Shaoyu Liu | 2022 | Bioactive Materials2022，7，3： | 3 |
| 2 | Transcatheter closure of interatrial communications for secondary prevention of paradoxical embolism: single-center experience显示文摘 | Bruch L Parsi A Grad MO | 2002 | Circulation2002，105，24： | 2 |
| 3 | Ageing affects chondroitin sulfates and their synthetic enzymes in the intervertebral disc显示文摘The depletion of chondroitin sulfates(CSs)within the intervertebral disc(IVD)during degenerative disc disease(DDD)results in a decrease in tissue hydration,a loss of fluid movement,cell apoptosis,a loss of nerve growth inhibition and ultimately,the loss of disc function.To date,little is known with regards to the structure and content of chondroitin sulfates(CSs)during IVD ageing.The behavior of glycosaminoglycans(GAGs),specifically CSs,as well as xylosyltransferase I(XT-I)and glucuronyltransferase I(GT-I),two key enzymes involved in CS synthesis as a primer of glycosaminoglycan(GAG)chain elongation and GAG synthesis in the nucleus pulposus(NP),respectively,were evaluated in a bovine ageing IVD model.Here,we showed significant changes in the composition of GAGs during the disc ageing process(6-month-old,2-year-old and 8-year-old IVDs representing the immature to mature skeleton).The CS quantity and composition of annulus fibrosus(AF)and NP were determined.The expression of both XT-I and GT-I was detected using immunohistochemistry.A significant decrease in GAGs was observed during the ageing process.CSs are affected at both the structural and quantitative levels with important changes in sulfation observed upon maturity,which correlated with a decrease in the expression of both XT-I and GT-I.A progressive switch of the sulfation profile was noted in both NP and AF tissues from 6 months to 8 years.These changes give an appreciation of the potential impact of CSs on the disc biology and the development of therapeutic approaches for disc regeneration and repair. | Estelle C Collin Oliver Carroll Michelle Kilcoyne Marianna Peroglio Eugene See Doris Hendig Mauro Alini Sibylle Grad Abhay Pandit | 2017 | Signal Transduction and Targeted Therapy2017，2，1： | 2 |
| 4 | 3,4,5-三甲氧基溴苯的合成显示文摘比较了几种 3,4,5 三甲氧基溴苯 (BTMOB)的合成方法。 3,4,5 三甲氧基苯胺 (TMOA)经重氮化和Sandmeyer法合成 ,BTMOB的收率较高。确定了最佳反应条件 :n(ArNH2 )∶n(NaNO2 )∶n(NaBr) =1 0∶1 2∶1 5 ,重氮化反应温度低于 5℃ ,重氮化络合物分解时 ,回流 1h ,BTMOB的收率可达 87 5 %。 | 史鸿鑫 林辉 Mandvill Grad Bloch Robert 刘化章 | 2001 | 精细化工2001，18，8： | 2 |
| 5 | Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration显示文摘It has been proven that the mechanical microenvironment can impact the differentiation of mesenchymal stem cells(MSCs).However,the effect of mechanical stimuli in biofabricating hydroxyapatite scaffolds on the inflammatory response of MSCs remains unclear.This study aimed to investigate the effect of mechanical loading on the inflammatory response of MSCs seeded on scaffolds.Cyclic mechanical loading was applied to biofabricate the cell-scaffold composite for 15 min/day over 7,14,or 21 days.At the predetermined time points,culture supernatant was collected for inflammatory mediator detection,and gene expression was analyzed by qRT-PCR.The results showed that the expression of inflammatory mediators(IL1B and IL8)was downregulated(p<0.05)and the expression of ALP(p<0.01)and COL1A1(p<0.05)was upregulated under mechanical loading.The cell-scaffold composites biofabricated with or without mechanical loading were freeze-dried to prepare extracellular matrix-based scaffolds(ECM-based scaffolds).Murine macrophages were seeded on the ECM-based scaffolds to evaluate their polarization.The ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying enhanced the expression of M2 polarization-related biomarkers(Arginase 1 and Mrc1,p<0.05)of macrophages in vitro and increased bone volume/total volume ratio in vivo.Overall,these findings demonstrated that mechanical loading could dually modulate the inflammatory responses and osteogenic differentiation of MSCs.Besides,the ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying facilitated the M2 polarization of macrophages in vitro and bone regeneration in vivo.Mechanical loading may be a promising biofabrication strategy for bone biomaterials. | Penghui Zhang Xizhe Liu Peng Guo Xianlong Li Zhongyuan He Zhen Li Martin J.Stoddart Sibylle Grad Wei Tian Dafu Chen Xuenong Zou Zhiyu Zhou Shaoyu Liu | 2021 | Bioactive Materials2021，6，10： | 2 |
| 6 | Identification of many microRNAs that copurify with polyribosomes in mammalian neurons显示文摘 | KIM J KRICHEVSKY A GRAD Y | 2004 | Proc Natl Acad Sci USA2004，101，： | 1 |
| 7 | Ascorbic acid enhances arsenic trioxide induced cytotoxicity in multiple myeloma cells显示文摘 | GRAD J M BAHLIS N J REIS I | 2001 | Blood2001，98，： | 1 |
| 8 | The capsular warning syn- drome : pathogenesis and clinical features 显示文摘 | Donnan GA 0 Malley HM Grad LQ | 1993 | Neurology1993，43，： | 1 |
| 9 | C-reactive protein and atherothrembosis: cause or effect 显示文摘 | Grad E Danenbery HD | 2013 | Blood Reviews2013，27，1： | 1 |
| 10 | The Economic Burden of Dry Eye: A Conceptual Framework and Preliminary Assessment显示文摘 | Prabashni Reddy Oren Grad Krithika Rajagopalan | 2004 | Cornea2004，，8： | 1 |
| 11 | Cells and biomaterials in cartilage tissue engineering显示文摘 | Stoddart M J Grad S Eglin D | 2009 | Regen Med2009，4，1： | 1 |
| 12 | Treatment options for multidrug-resistant acinetobacter species显示文摘 | GRad J Carmeli Y | 2008 | Drugs2008，68，2： | 1 |
| 13 | Cells and biomaterials for inter-vertebral disc regeneration显示文摘 | Grad S Alini M Eg^in D | 2010 | Synth Lect Tissue Eng2010，2，1： | 1 |
| 14 | Effects of immobilization and dynamic compression on intervertebral disc cell gene expression in vivo显示文摘 | MacLean J Lee C Grad S | 2003 | Spine2003，28，： | 1 |
| 15 | C - reactive protein and atherothrombosis-a prognostic factor or a risk factor 显示文摘 | Yosef - LeviIM Grad E Danenberg HD | 2007 | Harefuah2007，146，12： | 1 |
| 16 | Genomic epidemiology of the Escherichia coli O104:H4 outbreaks in Europe, 2011 显示文摘 | Grad YH Lipsitch M Feldgarden M | 2012 | Proc Natl Acad Sci2012，109，8： | 1 |
| 17 | The capsular warning syndrome:pathogenesis and clinical features显示文摘 | Donnan GA O'Malley HM Grad LQ | 1993 | Neurology1993，43，： | 1 |
| 18 | Asymptotic theory of the boltzmann equation显示文摘 | Grad H | 1963 | Physics of Fluids1963，6，： | 1 |
| 19 | Beneficial and detrimental effects of UV on aquatic organisms:implications of spectral variation显示文摘 | Williamson C E Neale P J Grad G | 2001 | Ecol Appl2001，11，： | 1 |
| 20 | Neointimal for- mation is reduced after arterial injury in human erp transgenic mice 显示文摘 | Danenberg HD Grad E Swaminathan RV | 2008 | Atheroselerosis2008，201，1： | 1 |
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